Difference between revisions of "Team:Pasteur Paris/Protocol"

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<!-- CONTENU DE LA PAGE DE GARDE CSS -->
 
<!-- CONTENU DE LA PAGE DE GARDE CSS -->
 
<div id="accueilScience">  
 
<div id="accueilScience">  
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<h1><B>Protocols</B></h1>
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<div class="text2">
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<p>During our lab work we used the following protocols. You can also download all the protocols in one file here:</p></br>
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</div>
 +
<div class="text1">
 +
<table>
 +
  <thead>
 +
    <tr>
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      <th>Experiments</th>
 +
      <th>Aim:</th>
 +
      <th>Download</th>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 +
      <td><strong>Microbiology equipment</strong></td>
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      <td>Materials always used in the lab </td>
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      <td> <center><a href="https://static.igem.org/mediawiki/2016/4/40/T--Pasteur_Paris--equipment.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong><p>PCR</p></strong></td>
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      <td>To reproduce (amplify) selected sections of DNA (inserts A1, A2, B1, B2, C1, C2, E1,E2).</td>
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      <td> <center><a href="https://static.igem.org/mediawiki/2016/b/be/T--Pasteur_Paris--PCR_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Digestion</strong></td>
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      <td>To linearize the different plasmids with appropriate enzymes. Perform restriction enzyme digestion in order to recover linear backbones of the plasmids. And chose the appropriate restriction sites based on the host plasmids. </td>
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      <td> <center><a href="https://static.igem.org/mediawiki/2016/a/ab/T--Pasteur_Paris--Restriction_digestion_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
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      <td><strong>Dephosphorylation</strong></td>
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      <td>To reduce vector self-ligation and favor that of vector to insert instead. </td>
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      <td> <center><a href="https://static.igem.org/mediawiki/2016/c/c0/T--Pasteur_Paris--dephosphorylation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Ligation</strong></td>
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      <td>To link an insert to its host plasmid before the transformation. This step happens after the digestion of the insert and the plasmid with the same restriction sites enzymes.
 +
</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/6/6f/T--Pasteur_Paris--Ligation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Electrophoresis</strong></td>
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      <td>To know the size of DNA fragments to check the efficiency of a digestion for instance.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/6/60/T--Pasteur_Paris--Gel_electrophoresis_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Gel extraction kit</strong></td>
 +
      <td>To get back the DNA purified thanks to the electrophoresis on agarose gel.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4e/T--Pasteur_Paris--Gel_extraction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Transformation</strong></td>
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      <td>To increase the amount of plasmid by transformation in competent cells. The amount of plasmid supplied is insufficient to perform all our future experiments.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/c/ca/T--Pasteur_Paris--Transformation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Miniprep QIAGEN Kit</strong></td>
 +
      <td> To extract DNA from a culture of bacteria</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/d/d5/T--Pasteur_Paris--Miniprep_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Harvest and culture</strong></td>
 +
      <td>In order to obtain a large amount of plasmid, we need to grow the bacteria overnight.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4b/T--Pasteur_Paris--Bacterial_culture_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
<tr>
 +
      <td><strong>Stab culture</strong></td>
 +
      <td>To store a clone of bacteria to be used later.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/b/b1/Stab_culture_Pasteur_Paris_2016.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>IPTG induction</strong></td>
 +
      <td>To increase the production of our protein which depends on this molecule.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/a/a4/T--Pasteur_Paris--Protein_induction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong>Protein Purification Fast Protein Liquid Chromatography</strong></td>
 +
      <td>To check if the His-tag works and if our protein has really been produced </td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/0/07/T--Pasteur_Paris--FPLC_Protein_purification_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong><p>Cellulose-binding Protocol</p></strong></td>
 +
      <td>Verify that the C2 protein binds to cellulose.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/e/e1/Protocol-cellulose-binding_Pasteur_Paris2016.pdf"><img src="https://static.igem.org/mediawiki/2016/1/15/T--Pasteur_Paris--Chemistry_T--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
 +
    </tr>
 +
    <tr>
 +
      <td><strong><p>Silification & assay Protocol</p></strong></td>
 +
      <td> Prepare a one pot mixture that will be compressed directly after incubation.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/2/2a/Protocol-silification-assay_Pasteur_Paris2016.pdf"><img src="https://static.igem.org/mediawiki/2016/1/15/T--Pasteur_Paris--Chemistry_T--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
    <td><strong><p>Model of the mechanical properties of the patch</p></strong></td>
 +
      <td>Predict the influence of silification on the mechanical properties of the patch</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/d/da/T--Pasteur_Paris--Mechanical_patch_properties_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/7/76/T--Pasteur_Paris--Patch_T--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
    <td><strong><p>Patch compression Protocol</p></strong></td>
 +
      <td>Make a patch by compression with cellulose and the silicated C2 protein.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/a/ac/Protocol-patch-compression_Pasteur_Paris.pdf"><img src="https://static.igem.org/mediawiki/2016/7/76/T--Pasteur_Paris--Patch_T--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong><p>One pot mixture-Patch protocol</p></strong></td>
 +
      <td> Prepare a one pot mixture that will be compressed directly after incubation.</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/8/89/Protocol-one-pot-mixture_Pasteur_Paris2016.pdf"><img src="https://static.igem.org/mediawiki/2016/7/76/T--Pasteur_Paris--Patch_T--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td>
 +
    </tr>
 +
    <tr>
 +
      <td><strong><p>Antibody-binding Protocol</p></strong></td>
 +
      <td> The goal is to determine whether our immunoassay is effective. We want to ensure that our antibodies are well able to detect the presence or absence of viral proteins from Chikungunya and Yellow Fever viruses.
 +
</td>
 +
      <td> <center><a href="https://static.igem.org/mediawiki/2016/2/21/T--Pasteur_Paris--Immunoassay_Protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/f/f1/T--Pasteur_Paris--Antibody_Pasteur_PARIS_2016.png" width="40%" alt=""></a></center></td>
 +
    </tr>
  
−
<article>
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  </tbody>
−
        <section><center><a href="#Science"><img src="https://static.igem.org/mediawiki/2016/b/b8/SCIENCE_PASTEUR_2016.png"class="image science"  alt=""></center></a></section>
+
</table>
  
 +
</br></br></br>
 +
</div>
 +
<div class="text2">
 +
<p></a>
 +
You can also download all the protocols in one file here:</br></br>
 +
<center><a href="https://static.igem.org/mediawiki/2016/6/66/T--Pasteur_Paris--Protocols.pdf"><img src="https://static.igem.org/mediawiki/2016/3/3a/T--Pasteur_Paris--Protocols_Pasteur_Paris_2016.png" width="10%" alt=""></a></center></br></br></br></br></br></br></p>
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</div>
  
−
</article>
+
<div class="text1">
 +
        <section><center><a href="#Science"><img src="https://static.igem.org/mediawiki/2016/c/c7/T--Pasteur_Paris--SCIENCE_PASTEUR_2016.png"class="image science"  alt=""></center></a></section>
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</div>
  
 
</div>
 
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Latest revision as of 23:52, 19 October 2016