Difference between revisions of "Team:IIT-Madras/Design"
Venkat1994 (Talk | contribs) |
|||
| (6 intermediate revisions by 4 users not shown) | |||
| Line 3: | Line 3: | ||
= RIBOS = | = RIBOS = | ||
== Introduction == | == Introduction == | ||
| − | + | In order to make precise and predictable biological devices, which can be controlled at will, we have designed a ribo-regulatory switch and named it RIBOS (RNA Inducible Boolean Output like Switch). RIBOS works on the principle of Watson-Crick base pairing between '''trigger RNA''' and '''switch mRNA'''. There are two sub-types of RIBOS, <span class="riboson">RIBOSON</span> and <span class="ribosoff">RIBOSOFF</span>, which activates and represses the translation process of downstream protein coding mRNA respectively in the presence of corresponding '''trigger RNA'''. The validation and functional optimization of RIBOS switches have been done with the help of 'Nupack' software by taking the thermal stability of RNA secondary structures and various other parameters into account. We have designed RIBOS such that the difference between it's ON and OFF states for both sub-types of switches are large enough to make them reliable in cell environment. Owing to the special designs of RIBOS, the base pairing between trigger and switch can range from 10 nucleotide to around 25 nucleotide, which makes it very orthogonal in principle. RIBOS can be used at the place of RBS parts in our expression systems, which can be controlled by supplying '''trigger RNA''' molecules. It's applications range from detection and quantification of mRNA molecules to the design of independent and modular genetic circuits in limitless number using forward engineering. As a deliverable, the algorithm can be used by future iGEM teams to design <span class="riboson">RIBOSON</span> and <span class="ribosoff">RIBOSOFF</span> for any trigger sequence. | |
| − | + | ||
| + | == RIBOSON == | ||
| + | [[Image:SingleMolDet_iGEM16.png|900px|center]] | ||
<gallery widths=275px heights=275px 'frameless'> | <gallery widths=275px heights=275px 'frameless'> | ||
| Line 13: | Line 14: | ||
</gallery> | </gallery> | ||
== RIBOSOFF == | == RIBOSOFF == | ||
| + | [[Image:SingleMolDet_iGEMRIBOSoff.png|900px|center]] | ||
<gallery widths=275px heights=275px 'frameless'> | <gallery widths=275px heights=275px 'frameless'> | ||
File:RIBOSOFF_s1.svg|Initial State | File:RIBOSOFF_s1.svg|Initial State | ||
| Line 19: | Line 21: | ||
</gallery> | </gallery> | ||
| + | Given below are animations which describe the working of <span class="riboson">RIBOSON</span> and <span class="ribosoff">RIBOSOFF</span> respectively. | ||
<html> | <html> | ||
<div style="display:inline-block;"> | <div style="display:inline-block;"> | ||
Latest revision as of 23:59, 19 October 2016
RIBOS
Introduction
In order to make precise and predictable biological devices, which can be controlled at will, we have designed a ribo-regulatory switch and named it RIBOS (RNA Inducible Boolean Output like Switch). RIBOS works on the principle of Watson-Crick base pairing between trigger RNA and switch mRNA. There are two sub-types of RIBOS, RIBOSON and RIBOSOFF, which activates and represses the translation process of downstream protein coding mRNA respectively in the presence of corresponding trigger RNA. The validation and functional optimization of RIBOS switches have been done with the help of 'Nupack' software by taking the thermal stability of RNA secondary structures and various other parameters into account. We have designed RIBOS such that the difference between it's ON and OFF states for both sub-types of switches are large enough to make them reliable in cell environment. Owing to the special designs of RIBOS, the base pairing between trigger and switch can range from 10 nucleotide to around 25 nucleotide, which makes it very orthogonal in principle. RIBOS can be used at the place of RBS parts in our expression systems, which can be controlled by supplying trigger RNA molecules. It's applications range from detection and quantification of mRNA molecules to the design of independent and modular genetic circuits in limitless number using forward engineering. As a deliverable, the algorithm can be used by future iGEM teams to design RIBOSON and RIBOSOFF for any trigger sequence.
RIBOSON
- Error creating thumbnail: File missing
Initial State
- Error creating thumbnail: File missing
Intermdiate State
- Error creating thumbnail: File missing
Final State
RIBOSOFF
- Error creating thumbnail: File missing
Initial State
- Error creating thumbnail: File missing
Intermediate State
- Error creating thumbnail: File missing
Final State
Given below are animations which describe the working of RIBOSON and RIBOSOFF respectively.
