Difference between revisions of "Team:CSU Fort Collins/Safety"

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<h1>Safety</h1>
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<h2> Safety in Design</h2>
  
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<p> For cloning ease, we utilized the laboratory <i>E. coli</i> strain DH5𝛼 as our chassis. The parts we built would then be tested primarily in our cyanobacteria <i>Synechocystis sp.</i> PCC 6803. Both organisms are non-pathogenic and do not survive well outside of laboratory or highly maintained conditions. If we are able to upscale production, we plan to build a photobioreactor to grow our CyanoLogic cyanobacteria. Compared to traditional open ponds systems, this method provides a more contained system.</p>
  
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<h2> Safety in the lab </h2>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<p>Prior to gaining entrance to our laboratory spaces, all members were required to become certified in the following online trainings through CSU’s Environmental and Health Services Department and CSU’s Research and Scholarly Ethics Department: Hazardous Waste Generator Training, BSL1 and BSL2 Training, and Institutional Responsible Conduct of Research Training.</p>
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<p>While performing experiments, we all wore standard personal protective equipment, including gloves.</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<p>We followed standard safety procedures required for Biosafety Level 1 facilities including wearing proper PPE equipment (gloves, safety glasses, and lab coats) for Biosafety Level 1 facilities. As for generated waste, we autoclave all biohazardous waste before it is placed in biohazardous waste bins to be disposed. Our lab work also adhered to <a href="https://drive.google.com/file/d/0BzRT2XYL-3jhS0NpUE5yOV9EREk/view
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" target="_blank">CSU’s biosafety guidelines.</a></p>
  
 
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Revision as of 00:15, 20 October 2016

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CSU iGEM



Safety



Safety in Design

For cloning ease, we utilized the laboratory E. coli strain DH5𝛼 as our chassis. The parts we built would then be tested primarily in our cyanobacteria Synechocystis sp. PCC 6803. Both organisms are non-pathogenic and do not survive well outside of laboratory or highly maintained conditions. If we are able to upscale production, we plan to build a photobioreactor to grow our CyanoLogic cyanobacteria. Compared to traditional open ponds systems, this method provides a more contained system.


Safety in the lab

Prior to gaining entrance to our laboratory spaces, all members were required to become certified in the following online trainings through CSU’s Environmental and Health Services Department and CSU’s Research and Scholarly Ethics Department: Hazardous Waste Generator Training, BSL1 and BSL2 Training, and Institutional Responsible Conduct of Research Training.

We followed standard safety procedures required for Biosafety Level 1 facilities including wearing proper PPE equipment (gloves, safety glasses, and lab coats) for Biosafety Level 1 facilities. As for generated waste, we autoclave all biohazardous waste before it is placed in biohazardous waste bins to be disposed. Our lab work also adhered to CSU’s biosafety guidelines.