Difference between revisions of "Team:DTU-Denmark/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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                        <h1>Safety<p class="lead">Working in the laboratory with different organisms and reagents may cause a threat to human health and the environment by wrong disposal of waste. With this in mind, we have chosen to work with a GRAS organism and have taken precausion while working in the lab</p></h1>
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                    <div class="col-md-5 col-sm-5 hidden-xs intro"> <!-- will be hidden on phones, duplicate the text to blockquote down below first section header, to show it there, when it dissapear-->
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                        <blockquote class="blockquote-reverse"> <!-- EDIT -->
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                            <p>"Safety is something that happens between your ears, not something you hold in your hands"</p>
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                            <small>Jeff Cooper</small>
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        <div><a class="anchor" id="section-1"></a>
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        <h2 class="h2">Precautionary Work</h2>
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                <p>"Safety is something that happens between your ears, not something you hold in your hands"</p>
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                <small>Jeff Cooper</small>
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            </blockquote>
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<p>To minimize the risks while working on the iGEM project we chose the chassis organism <i>Yarrowia lipolytica</i> which is generally regarded as safe (GRAS) and non-pathogenic to humans. Aside from that we worked with the <i>Escherichia coli</i> DH5 alpha laboratory strain which does not pose a threat to healthy humans.  We also did some growth experiments with  <i>Saccharomyces cerevisiae</i>, another GRAS organism. </p>  
  
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<p>In our choice of genetic material we considered the risks the origin organism could pose. We only used genes from safe sources such as <i>Xanthophyllomyces dendrorhous</i>, <i>Renilla reinformis</i> and the human gene for pre-insulin codon optimised with our own codon-optimization tool for <i>Y. lipolityca</i>.</p>
  
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<h5>Safe Project Design</h5>
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        <h2 class="h2">Laboratory Safety Measures</h2>
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          <p>All work with genetically modified organisms (GMOs) were performed in class 1 GMO laboratories following the required procedures. This include amongst others wearing laboratory coats, gloves and goggles, disinfecting surfaces, tools etc. with ethanol and autoclaving all waste, used tools and discarded cultures. We used laf-benches whenever possible to avoid the fire hazard of gas flames when performing sterile work. The Hamilton robot used as fermentation platform was decontaminated before and after each use with UV light.</p>
  
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<p>In our work with hazardous substances we took extra safety measures and worked with utmost caution. We used laf-benches and powerful suction in the experiments performed with 2-Mercaptoethanol (BME). SYBR-Safe was always handled with gloves and caution. Containers with liquid nitrogen were only opened and cooling conducted in well ventilated rooms.
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            <li><a href="#section-1">Precautionary work</a></li>
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            <li><a href="#section-2">Laboratory safety measures</a></li>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 00:18, 20 October 2016

New HTML template for the wiki




Bootstrap Example

Safety

Working in the laboratory with different organisms and reagents may cause a threat to human health and the environment by wrong disposal of waste. With this in mind, we have chosen to work with a GRAS organism and have taken precausion while working in the lab


Precautionary Work

"Safety is something that happens between your ears, not something you hold in your hands"

Jeff Cooper

To minimize the risks while working on the iGEM project we chose the chassis organism Yarrowia lipolytica which is generally regarded as safe (GRAS) and non-pathogenic to humans. Aside from that we worked with the Escherichia coli DH5 alpha laboratory strain which does not pose a threat to healthy humans. We also did some growth experiments with Saccharomyces cerevisiae, another GRAS organism.

In our choice of genetic material we considered the risks the origin organism could pose. We only used genes from safe sources such as Xanthophyllomyces dendrorhous, Renilla reinformis and the human gene for pre-insulin codon optimised with our own codon-optimization tool for Y. lipolityca.

Laboratory Safety Measures

All work with genetically modified organisms (GMOs) were performed in class 1 GMO laboratories following the required procedures. This include amongst others wearing laboratory coats, gloves and goggles, disinfecting surfaces, tools etc. with ethanol and autoclaving all waste, used tools and discarded cultures. We used laf-benches whenever possible to avoid the fire hazard of gas flames when performing sterile work. The Hamilton robot used as fermentation platform was decontaminated before and after each use with UV light.

In our work with hazardous substances we took extra safety measures and worked with utmost caution. We used laf-benches and powerful suction in the experiments performed with 2-Mercaptoethanol (BME). SYBR-Safe was always handled with gloves and caution. Containers with liquid nitrogen were only opened and cooling conducted in well ventilated rooms.

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