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− | <p class="text-muted">With our exams and assignments from semester 2 complete we set about outlining the details of the team's project and identifying a dissertation subject for each team member.</p> | + | <p class="text-muted"> |
+ | <br>With our exams and assignments from semester 2 complete we set about outlining the details of the team's project and identifying a dissertation subject for each team member. | ||
+ | <br>The team met with Jane Calvert to discuss the stakeholders in the project, including industry, research teams, other iGEM team and the public. The consideration of safety issues surrounding the use of heterologous parts in uncharacterised hosts and potentially unseen hazardous consequences sprang from this meeting and was expanded on throughout our project.<br> | ||
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<br> Mutagenesis was conducted to remove illegal sites from the Rhodococcus vectors. Mut2 and Mut3 showed a good result.<br> | <br> Mutagenesis was conducted to remove illegal sites from the Rhodococcus vectors. Mut2 and Mut3 showed a good result.<br> | ||
<br> Our fungi team worked on their MoClo protocols in order to start assembling devices with our the designed PhytoBricks and achieved great efficiency at LV0. We also worked on optimisation of transformation protocol in P. roqueforti.<br> | <br> Our fungi team worked on their MoClo protocols in order to start assembling devices with our the designed PhytoBricks and achieved great efficiency at LV0. We also worked on optimisation of transformation protocol in P. roqueforti.<br> | ||
+ | <br> Jane Calvert and Deborah Scott helped the group to delve deeper in to the social dimensions of the project and human practices, with discussion focused on new questions of patents, GMOs and ethics that powerful and enabling genetic technologies such as CRISPR/Cas have brought up.<br> | ||
+ | <br> The first microorganisms for the CARE tool were chosen and its secondary metabolites retrieved. <br> | ||
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<br> All of our designed parts were shipped to the lab and were then cloned into the PhytoBrick Universal Acceptor Vector. | <br> All of our designed parts were shipped to the lab and were then cloned into the PhytoBrick Universal Acceptor Vector. | ||
<br> The fungi team designed multiple gBlocks fragments for synthesis of the AMA1 sequence that was to be assembled using a Gibson strategy. Problems were encountered both with the transformation of P. roqueforti and the assembly of LV1 parts.<br> | <br> The fungi team designed multiple gBlocks fragments for synthesis of the AMA1 sequence that was to be assembled using a Gibson strategy. Problems were encountered both with the transformation of P. roqueforti and the assembly of LV1 parts.<br> | ||
+ | <br> The data mining for the CARE tool database began. <br> | ||
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<br> After handing in our dissertations (what a relief to be done!) we’re straight back to work on the characterisation of our parts in our new home in Chris French’s lab. | <br> After handing in our dissertations (what a relief to be done!) we’re straight back to work on the characterisation of our parts in our new home in Chris French’s lab. | ||
<br> We also said goodbye to the team members who had to return home or start new jobs. The remaining members worked hard on characterising the Sac-6 promoter.<br> | <br> We also said goodbye to the team members who had to return home or start new jobs. The remaining members worked hard on characterising the Sac-6 promoter.<br> | ||
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− | <br> During this time we focused on expanding our collaborations with the University of Newcastle and Dundee High School iGEM teams. | + | <br> During this time we focused on expanding our collaborations with the University of Newcastle and Dundee High School iGEM teams. We also spread news of our CARE tool and engaged 20+ teams in our chassis safety assessment. |
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Latest revision as of 00:29, 20 October 2016
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