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<br> Our fungi team worked on their MoClo protocols in order to start assembling devices with our the designed PhytoBricks and achieved great efficiency at LV0. We also worked on optimisation of transformation protocol in P. roqueforti.<br> | <br> Our fungi team worked on their MoClo protocols in order to start assembling devices with our the designed PhytoBricks and achieved great efficiency at LV0. We also worked on optimisation of transformation protocol in P. roqueforti.<br> | ||
<br> Jane Calvert and Deborah Scott helped the group to delve deeper in to the social dimensions of the project and human practices, with discussion focused on new questions of patents, GMOs and ethics that powerful and enabling genetic technologies such as CRISPR/Cas have brought up.<br> | <br> Jane Calvert and Deborah Scott helped the group to delve deeper in to the social dimensions of the project and human practices, with discussion focused on new questions of patents, GMOs and ethics that powerful and enabling genetic technologies such as CRISPR/Cas have brought up.<br> | ||
+ | <br> The first microorganisms for the CARE tool were chosen and its secondary metabolites retrieved. <br> | ||
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<br> All of our designed parts were shipped to the lab and were then cloned into the PhytoBrick Universal Acceptor Vector. | <br> All of our designed parts were shipped to the lab and were then cloned into the PhytoBrick Universal Acceptor Vector. | ||
<br> The fungi team designed multiple gBlocks fragments for synthesis of the AMA1 sequence that was to be assembled using a Gibson strategy. Problems were encountered both with the transformation of P. roqueforti and the assembly of LV1 parts.<br> | <br> The fungi team designed multiple gBlocks fragments for synthesis of the AMA1 sequence that was to be assembled using a Gibson strategy. Problems were encountered both with the transformation of P. roqueforti and the assembly of LV1 parts.<br> | ||
+ | <br> The data mining for the CARE tool database began. <br> | ||
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− | <br> During this time we focused on expanding our collaborations with the University of Newcastle and Dundee High School iGEM teams. | + | <br> During this time we focused on expanding our collaborations with the University of Newcastle and Dundee High School iGEM teams. We also spread news of our CARE tool and engaged 20+ teams in our chassis safety assessment. |
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Latest revision as of 00:29, 20 October 2016
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