Difference between revisions of "Team:Paris Bettencourt/Parts"

 
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<h2>Medal Requirements</h2>
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<h2 class="red">Our Library of Standard Biological Parts</h2>
  
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     <th Colspan="4" style="background-color:#8B1813; color:#FFFFFF; font-size:20px;">Enzymes for Stain Digestion</th>
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     <div class=text>
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  </tr>
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    <h3 style="text-align:center">Bronze Medal</h3>
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  <tr style="background-color:#138B3F">
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        <ul class="checkmark" style="">
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    <th style="background-color:#DDDDDD">BioBrick ID</th>
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          <li>We registered our team, and had a great summer! :)</li>
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    <th style="background-color:#DDDDDD">Enzyme name</th>
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          <li>We have met all <a href="https://2016.igem.org/Requirements">deliverables</a></li>
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    <th style="background-color:#DDDDDD">Description</th>
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          <li>The attributions of every team member are available <a href="https://2016.igem.org/Team:Paris_Bettencourt/Attributions">here</a></li>
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    <th style="background-color:#DDDDDD">Reference</th>
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  <li>We have created, submitted and documented a dozen BioBricks. One of them is <a href="http://parts.igem.org/Part:BBa_K2043002">BBa_K2043002</a> which represents our contribution for the bronze medal.</li>
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   </tr>
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        </ul>
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    </div>
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     <td><a href="http://parts.igem.org/Part:BBa_K2043001">BBa_K2043001</a></td>
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     <div class=text>
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    <td>catA</td>
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    <h3 style="text-align:center">Silver Medal</h3>
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    <td style="text-align: justify"><i>catA</i>, Catechol-1,2-dihydrogenase from <i>Acinetobacter pittii</i> codon optimised for <i>E. coli</i>. </td>
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        <ul class="checkmark" style="">
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    <td style="text-align: justify">DOI 10.1007/s10930-015-9637-7</td>
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          <li><a href="http://parts.igem.org/Part:BBa_K2043004">BBa_K2043004</a> is a xylE gene from <i>Pseudomonas putida</i> codon optimized for expression in <i>E. coli</i>; a new and well documented standard biological part. Our contribution for the silver medal requirements.</li>
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  </tr>
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          <li>Paris Bettencourt iGEM team has made many many friends over the summer. Our colaborations are listed <a href="https://2016.igem.org/Team:Paris_Bettencourt/Collaborations">here</a>.</li>
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          <li>Our project tackled a serious issue of perchlorethylene (PERC) poisoning in the dry-cleaning facilities. On this <a href="https://2016.igem.org/Team:Paris_Bettencourt/HP/Silver">link</a> you can read more about this problem: how we identified, investigated and addressed it.</li>
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  <tr>
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        </ul>
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    <td><a href="http://parts.igem.org/Part:BBa_K2043002">BBa_K2043002</a></td>
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     </div>
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    <td>catA-FBD1</td>
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  </div>
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    <td style="text-align: justify"><i>catA</i>, Catechol-1,2-dihydrogenase from <i>Acinetobacter pittii</i> codon optimised for <i>E. coli</i> and fused on the N-terminal with FBD1 </td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2043003">BBa_K2043003</a></td>
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     <div class=text>
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    <td>catA-FBD10</td>
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     <h3 style="text-align:center">Gold Medal</h3>
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    <td style="text-align: justify"><i>catA</i>, Catechol-1,2-dihydrogenase from <i>Acinetobacter pittii</i> codon optimised for <i>E. coli</i> and fused on the N-terminal with FBD10 </td>
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      <p>
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    <td style="text-align: justify"></td>
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        <ul class="checkmark" style="">
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   </tr>
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          <li>On this <a href="https://2016.igem.org/Team:Paris_Bettencourt/HP/Gold">link</a> we present how the PERC poisoning problem was integrated into our project. How we designed and addressed the issue through our project.</li>
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          <li>We have also improved the characterization of two previously existing BioBricks (BBa_K1321357 and BBa_K863001). For the gold medal requirements we are providing the <a href="https://2016.igem.org/Team:Paris_Bettencourt/Description">description</a> of the improvements made for <a href="http://parts.igem.org/Part:BBa_K1321357">BBa_K1321357</a>, a BioBrick designed by iGEM team of Imperial College London in 2014.</li>
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    <tr>
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          <li>The <a href="https://2016.igem.org/Team:Paris_Bettencourt/Proof">proof of concept</a> shows that our project can be implemented ???? I don't know what to write here :(</li>
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    <td><a href="http://parts.igem.org/Part:BBa_K2043004">BBa_K2043004</a></td>
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        </ul>
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    <td>xylE</td>
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      </p>
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    <td style="text-align: justify"><i>xylE</i>, Catechol-2,3-dihydrogenase from <i>Pseudomonas putida</i> codon optimised for <i>E. coli</i> </td>
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     </div>
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    <td style="text-align: justify">Eur. J. Biochem. 229, 113-118 (1995)</td>
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   </div>
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   </tr>
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</div>
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</div>
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    <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2043005">BBa_K2043005</a></td>
 +
     <td>xylE-FBD1</td>
 +
    <td style="text-align: justify"><i>xylE</i>, Catechol-2,3-dihydrogenase from <i>Pseudomonas putida</i> codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD1 </td>
 +
     <td style="text-align: justify"></td>
 +
  </tr>
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 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043006">BBa_K2043006</a></td>
 +
    <td>xylE-FBD10</td>
 +
    <td style="text-align: justify"><i>xylE</i>, Catechol-2,3-dihydrogenase from <i>Pseudomonas putida</i> codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD10 </td>
 +
    <td style="text-align: justify"></td>
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  </tr>
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 +
    <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2043007">BBa_K2043007</a></td>
 +
    <td>bpuI</td>
 +
    <td style="text-align: justify"><i>bpuI</i>, laccase from <i>Bacillus pumillus</i> codon optimised for <i>E. coli</i> </td>
 +
    <td style="text-align: justify">DOI 10.1016/j.biotechadv.2006.04.003</td>
 +
  </tr>
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 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043008">BBa_K2043008</a></td>
 +
    <td>bpuI-FBD1</td>
 +
    <td style="text-align: justify"><i>bpuI</i>, laccase from <i>Bacillus pumillus</i> codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD1</td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043009">BBa_K2043009</a></td>
 +
    <td>bpuI-FBD10</td>
 +
    <td style="text-align: justify"><i>bpuI</i>, laccase from <i>Bacillus pumillus</i> codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD10</td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043010">BBa_K2043010</a></td>
 +
    <td>GFP-FBD1</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD1 </td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043011">BBa_K2043011</a></td>
 +
    <td>GFP-FBD2</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD2 </td>
 +
    <td style="text-align: justify"</td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043012">BBa_K2043012</a></td>
 +
    <td>GFP-FBD5</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD5 </td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
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 +
    <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2043013">BBa_K2043013</a></td>
 +
    <td>GFP-FBD6</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD6</td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043014">BBa_K2043014</a></td>
 +
    <td>GFP-FBD7</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD7 </td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043015">BBa_K2043015</a></td>
 +
    <td>GFP-FBD8</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD8 </td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
 +
 
 +
     <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043016">BBa_K2043016</a></td>
 +
    <td>GFP-FBD9</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD9 </td>
 +
    <td style="text-align: justify"></td>
 +
   </tr>
 +
 
 +
    <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2043017">BBa_K2043017</a></td>
 +
    <td>GFP-FBD10</td>
 +
    <td style="text-align: justify">GFP gene, codon optimised for <i>E. coli</i> and fused in the N-terminal with FBD10 </td>
 +
    <td style="text-align: justify"></td>
 +
  </tr>
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Latest revision as of 00:36, 20 October 2016


Our Library of Standard Biological Parts

Enzymes for Stain Digestion
BioBrick ID Enzyme name Description Reference
BBa_K2043001 catA catA, Catechol-1,2-dihydrogenase from Acinetobacter pittii codon optimised for E. coli. DOI 10.1007/s10930-015-9637-7
BBa_K2043002 catA-FBD1 catA, Catechol-1,2-dihydrogenase from Acinetobacter pittii codon optimised for E. coli and fused on the N-terminal with FBD1
BBa_K2043003 catA-FBD10 catA, Catechol-1,2-dihydrogenase from Acinetobacter pittii codon optimised for E. coli and fused on the N-terminal with FBD10
BBa_K2043004 xylE xylE, Catechol-2,3-dihydrogenase from Pseudomonas putida codon optimised for E. coli Eur. J. Biochem. 229, 113-118 (1995)
BBa_K2043005 xylE-FBD1 xylE, Catechol-2,3-dihydrogenase from Pseudomonas putida codon optimised for E. coli and fused in the N-terminal with FBD1
BBa_K2043006 xylE-FBD10 xylE, Catechol-2,3-dihydrogenase from Pseudomonas putida codon optimised for E. coli and fused in the N-terminal with FBD10
BBa_K2043007 bpuI bpuI, laccase from Bacillus pumillus codon optimised for E. coli DOI 10.1016/j.biotechadv.2006.04.003
BBa_K2043008 bpuI-FBD1 bpuI, laccase from Bacillus pumillus codon optimised for E. coli and fused in the N-terminal with FBD1
BBa_K2043009 bpuI-FBD10 bpuI, laccase from Bacillus pumillus codon optimised for E. coli and fused in the N-terminal with FBD10
BBa_K2043010 GFP-FBD1 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD1
BBa_K2043011 GFP-FBD2 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD2
BBa_K2043012 GFP-FBD5 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD5
BBa_K2043013 GFP-FBD6 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD6
BBa_K2043014 GFP-FBD7 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD7
BBa_K2043015 GFP-FBD8 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD8
BBa_K2043016 GFP-FBD9 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD9
BBa_K2043017 GFP-FBD10 GFP gene, codon optimised for E. coli and fused in the N-terminal with FBD10
Results
Medal Requirements
Parts
Interlab Study
Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
igem2016parisbettencourt@gmail.com
2016.igem.org