Difference between revisions of "Team:SCUT-China A/Achievement part"

Latest revision as of 00:41, 20 October 2016

index

Type

Function

Length

dszA (a monooxygenase gene )

coding

convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway

1383 bp

BBa_K1972000

dszB (a desulfinase gene

coding

convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway

1117 bp

BBa_K1972001

dszC (a monooxygenase gene )

coding

not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway

1274 bp

BBa_K1972002

dszD (a flavinreductase)

coding

three successive oxidation steps require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) during the 4S pathway

599 bp

BBa_K1972003

dszA (a monooxygenase gene ), E.coli-optimized

coding

convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway

1383 bp

BBa_K1972004

dszB (a desulfinase gene), E.coli-optimized

coding

convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway

1170 bp

BBa_K1972005

dszC (a monooxygenase gene ), E.coli-optimized

coding

not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway

1274 bp

BBa_K1972006

dszBCAD with T7 promoter

coding

dszBCAD genes with T7 promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

4537 bp

BBa_K1972007

dszB with tac promoter induced by IPTG

Composite

dszB gene with tac promoter and terminator which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway.

1307 bp

BBa_K1972008

dszBCAD with Lac promoter

coding

dszBCAD genes with Lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

5859 bp

BBa_K1972009

dszBAC with tac promoter and dszD with lac promoter

coding

dszBAC genes with tac promoter and dszD gene with lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

6052 bp

BBa_K1972013

dszBBAC with tac promoter and dszD with lac promotor induced by IPTG

composite

dszBBAC with tac promoter and dszD with lac promotor induced by IPTG which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

7367 bp

BBa_K1972014

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Sulfur Killer

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South China University of tecnology

GuangZhou,China

Achievement parts

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 return _creator();
 })());
@@ Line 4,833: / Line 4,833: @@
            <!-- Unnamed () -->
            <div id="u2478" class="text" style="visibility: visible;">
-             <p><span>T7 RNA polymerase induced by IPTG</span></p>
+             <p><span>dszB with tac promoter induced by IPTG </span></p>
            </div>
          </div>
@@ Line 4,842: / Line 4,842: @@
            <!-- Unnamed () -->
            <div id="u2480" class="text" style="visibility: visible;">
-             <p><span>coding</span></p>
+             <p><span>Composite</span></p>
            </div>
          </div>
@@ Line 4,851: / Line 4,851: @@
            <!-- Unnamed () -->
            <div id="u2482" class="text" style="visibility: visible;">
-             <p><span>T7 RNA polymerase will be expressed with IPTG added.</span></p>
+             <p><span>dszB gene with tac promoter and terminator which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway.
+</span></p>
            </div>
          </div>
@@ Line 4,860: / Line 4,861: @@
            <!-- Unnamed () -->
            <div id="u2484" class="text" style="visibility: visible;">
-             <p><span>4552 bp</span></p>
+             <p><span>1307 bp</span></p>
            </div>
          </div>
@@ Line 4,968: / Line 4,969: @@
            <!-- Unnamed () -->
            <div id="u2511" class="text" style="visibility: visible;">
-             <p><span>dszBCAD with T7 promoter induced by IPTG</span></p>
+             <p><span>dszBBAC with tac promoter and dszD with lac promotor induced by IPTG </span></p>
            </div>
          </div>
@@ Line 4,986: / Line 4,987: @@
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            <div id="u2515" class="text" style="visibility: visible;">
-             <p><span>When IPTG added, dszBCAD genes with T7 promoter will be expressed greatly&nbsp; which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)</span></p>
+             <p><span>dszBBAC with tac promoter and dszD with lac promotor induced by IPTG which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)</span></p>
            </div>
          </div>
@@ Line 4,995: / Line 4,996: @@
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            <div id="u2517" class="text" style="visibility: visible;">
-             <p><span>9097 bp</span></p>
+             <p><span>7367 bp</span></p>
            </div>
          </div>
@@ Line 5,045: / Line 5,046: @@
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                    <div id="u2530" class="text" style="visibility: visible;">
-                     <p><span>Design&amp;Result</span></p>
+                     <p><span>Design&amp;Results</span></p>
                    </div>
                  </div>
@@ Line 5,202: / Line 5,203: @@
              <!-- Unnamed () -->
              <div id="u2563" class="text" style="visibility: visible;">
-               <p><span>Achievement</span></p>
+               <p><span>Achievements</span></p>
              </div>
            </div>
@@ Line 5,409: / Line 5,410: @@
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            <div id="u2608" class="text" style="visibility: visible;">
-             <p style="font-size:36px;"><span style="font-family:'Comic Sans MS Normal', 'Comic Sans MS';font-weight:400;">Sulffur Killer</span><span style="font-family:'Comic Sans MS Negreta', 'Comic Sans MS Normal', 'Comic Sans MS';font-weight:700;font-size:28px;"></span></p><p style="font-size:28px;"><span style="font-family:'Comic Sans MS Negreta', 'Comic Sans MS Normal', 'Comic Sans MS';font-weight:700;">wiki group suport</span><span style="font-family:'Arial Normal', 'Arial';font-weight:400;"> </span></p>
+             <p style="font-size:36px;"><span style="font-family:'Comic Sans MS Normal', 'Comic Sans MS';font-weight:400;">Sulfur Killer</span><span style="font-family:'Comic Sans MS Negreta', 'Comic Sans MS Normal', 'Comic Sans MS';font-weight:700;font-size:28px;"></span></p><p style="font-size:28px;"><span style="font-family:'Comic Sans MS Negreta', 'Comic Sans MS Normal', 'Comic Sans MS';font-weight:700;">wiki group suport</span><span style="font-family:'Arial Normal', 'Arial';font-weight:400;"> </span></p>
            </div>
          </div>