Difference between revisions of "Team:Aix-Marseille"
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| + | <h1><a href="#about">Highway to Platinum <i class="fa fa-chevron-right" aria-hidden="true"></i></a></h1> | ||
| + | <h2>Converting pollution into ressources</h2> | ||
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Indeed, platinum is present in the catalytic converters of cars and trucks and it is released progressively in the exhausts. Therefore the platinum accumulates around the traffic routes, on asphalt, in soil, even in plants. | Indeed, platinum is present in the catalytic converters of cars and trucks and it is released progressively in the exhausts. Therefore the platinum accumulates around the traffic routes, on asphalt, in soil, even in plants. | ||
| − | To safely exploit this resource, we imagined a concentrating system that could be integrated into existing water processing and phytoremediation systems. | + | To safely exploit this resource, we imagined a [https://2016.igem.org/Team:Aix-Marseille/Description concentrating system] that could be integrated into existing [https://2016.igem.org/Team:Aix-Marseille/Integrated_Practices/Environment water processing and phytoremediation systems.] |
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The first step relies on the affinity of siderophores to bind solubilized Platinum atoms and, thus, favor the further solubilisation of more platinum compounds. We accomplish this by inserting a plasmid containing the four enzymes (Des A, Des B, Des C, Des D) necessary to synthesise our siderophore — Desferrioxamine B, into E. coli. | The first step relies on the affinity of siderophores to bind solubilized Platinum atoms and, thus, favor the further solubilisation of more platinum compounds. We accomplish this by inserting a plasmid containing the four enzymes (Des A, Des B, Des C, Des D) necessary to synthesise our siderophore — Desferrioxamine B, into E. coli. | ||
| − | At a second level, we plan to use the principle of biosorption. A modified FliC protein complex will be cloned into E. coli and enable the flagella of the bacterium to bind platinum atoms. This specificity will be possible thanks to a peptide that will be inserted into the sequence of the FliC protein. | + | At a second level, we plan to use the [https://2016.igem.org/Team:Aix-Marseille/Design principle of biosorption]. A modified FliC protein complex will be cloned into E. coli and enable the flagella of the bacterium to bind platinum atoms. This specificity will be possible thanks to a peptide that will be inserted into the sequence of the [https://2016.igem.org/Team:Aix-Marseille/Composite_Part#BBa_K1951008_:_FliC_E._coli_producer FliC protein]. |
The benefit of using the biosorption is to obtain nanoparticles of platinum, a highly valuable form of the metal. | The benefit of using the biosorption is to obtain nanoparticles of platinum, a highly valuable form of the metal. | ||
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