Difference between revisions of "Team:Exeter/Safety"

 
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<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Human_Practices">Human Practices Homepage</a></li>
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    <li><a id="links" style="margin:10px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Integrated_Practices">Integrated</a></li>
    <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Integrated_Practices">Integrated</a></li>
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<li><a id="links" style="background:none;line-height:0.7vh;margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Engagement">Public Engagement<br /><br /><br /> & Education</a></li>
 
<li><a id="links" style="background:none;line-height:0.7vh;margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Engagement">Public Engagement<br /><br /><br /> & Education</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Log">Log</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Log">Log</a></li>
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<p id ="pp">Prior to beginning the laboratory work for our project, we all received a laboratory induction with Dagmara Kolak, the Mezzanine laboratory manager. As in standard with Biosafety Level 2 (BSL-2) laboratories, we signed the relevant lab safety paperwork and were guided through standard safety procedures. We were shown around the laboratory and introduced to the academics working in the laboratory, shown the locations of equipment and how to safely use it, and informed of the standard safety procedures for the Mezzanine laboratory.</p>
 
<p id ="pp">Prior to beginning the laboratory work for our project, we all received a laboratory induction with Dagmara Kolak, the Mezzanine laboratory manager. As in standard with Biosafety Level 2 (BSL-2) laboratories, we signed the relevant lab safety paperwork and were guided through standard safety procedures. We were shown around the laboratory and introduced to the academics working in the laboratory, shown the locations of equipment and how to safely use it, and informed of the standard safety procedures for the Mezzanine laboratory.</p>
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<p id = "pp"> All the appropriate COSHH forms were read and signed according to best laboratory practises and university policies. </p>
  
 
<h5>Laboratory Safety</h5>
 
<h5>Laboratory Safety</h5>
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<p id ="pp">In the Mezzanine laboratory, we worked alongside Biosciences researchers from different backgrounds, and were given our own bench, storage areas in the freezers are cold room, and full access to the equipment in the laboratory. As the Mezzanine laboratory is a BSL-2 laboratory, Risk Group 2 microorganisms were in use. Risk Group 2 microorganisms pose moderate risk to personnel and environment, so we had to follow strict safety procedures to prevent release of biomaterials and/or microorganisms into the environment.</p>
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<p id ="pp">In the Mezzanine laboratory, we worked alongside Biosciences researchers from different backgrounds, and were given our own bench, storage areas in the freezers and cold room. We were also given full access to the equipment in the laboratory. As the Mezzanine laboratory is a BSL-2 laboratory, we had to follow strict safety procedures to prevent release of biomaterials and/or microorganisms into the environment.</p>
  
<p id ="pp">During our project, we used <i>E. coli</i> strains BL21 DE3 and DH5α - both of these strains are non-pathogenic K-12-derived strains (), and are classified as Risk Group 1 microorganisms (eur-lex.europa.eu, 2000). Risk Group 1 microorganisms do not cause disease in healthy adult humans, and can be used in a BSL-1 laboratory (2016.igem.uk, 2016). </p>
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<p id ="pp">During our project, we used <i>E. coli</i> strains BL21 DE3 and DH5α - They are classified as Risk Group 1 microorganisms (eur-lex.europa.eu, 2000). Risk Group 1 microorganisms do not cause disease in healthy adult humans, and can be used in a BSL-1 laboratory (2016.igem.uk, 2016). </p>
  
 
<p id ="pp">To ensure we worked safely, and to prevent the possible release of biomaterials into the environment, we followed the procedures below:</p>
 
<p id ="pp">To ensure we worked safely, and to prevent the possible release of biomaterials into the environment, we followed the procedures below:</p>

Latest revision as of 01:00, 20 October 2016