Difference between revisions of "Team:Exeter/Safety"

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{{Exeter}}
 
 
<html>
 
<html>
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</div></div></div></div></div>
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  <meta charset="utf-8">
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  <meta name="viewport" content="width=device-width, initial-scale=1">
 +
  <link rel="stylesheet" href="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.6/css/bootstrap.min.css">
 +
  <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.12.4/jquery.min.js"></script>
 +
  <script src="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.6/js/bootstrap.min.js"></script>
 +
 
 +
 +
</head>
 +
 +
<body>
 +
<style type="text/css">
 +
  @media (max-width: 920px) {
 +
  .navbar-header {
 +
      float: none;
 +
  }
 +
  .navbar-left,.navbar-right {
 +
      float: none !important;
 +
  }
 +
  .navbar-toggle {
 +
      display: block;
 +
  }
 +
  .navbar-collapse {
 +
      box-shadow: inset 0 0 0 rgba(255,255,255,0.1);
 +
  }
 +
  .navbar-fixed-top {
 +
      top: 0;
 +
      border-width: 0 0 1px;
 +
  }
 +
  .navbar-collapse.collapse {
 +
      display: none!important;
 +
  }
 +
 
 +
  .navbar-nav {
 +
      float: none!important;
 +
      margin-top: 7.5px;
 +
  }
 +
  .navbar-nav>li {
 +
      float: none;
 +
  }
 +
  .navbar-nav>li>a {
 +
      padding-top: 10px;
 +
      padding-bottom: 10px;
 +
  }
 +
  .collapse.in{
 +
      display:block !important;
 +
  }
 +
  #soc_1, #soc_2, #soc_3{
 +
display:none;
 +
  }
 +
}
 +
#links{
 +
color:#47BCC2;
 +
/*font-size:20px;*/
 +
font-size:1.2vw;
 +
margin:0 0.9vw 0.5vh 0.9vw;
 +
padding:0;
 +
line-height:0px;
 +
padding-top:28px;
 +
}
 +
/*Fixes font size when nav-bar is collapsed*/
 +
@media (max-width: 920px) {
 +
#links{
 +
font-size:20px;
 +
}
 +
#links_drop{
 +
margin:0.9vw -0.4vw 0 -0.2vw;
 +
}
 +
}
 +
#links:hover{
 +
color:#339499;
 +
}
 +
#links:active{
 +
color:#339499;
 +
}
 +
.navbar{
 +
background-color:#e8e8e8;
 +
border-bottom:none;
 +
min-height:54px;
 +
    margin:4px 0 0 0;
 +
}
 +
.navbar-inverse .navbar-toggle .icon-bar {/*teal toggle */
 +
    background-color:#47BCC2;
 +
}
 +
.navbar-inverse .navbar-toggle { /*Removes toggle bobrder*/
 +
border:none;
 +
background-color:#e8e8e8;
 +
}
 +
.navbar-inverse .navbar-toggle{ /*Removes toggle bobrder*/
 +
border:none;
 +
background-color:#e8e8e8;
 +
}
 +
.navbar-inverse .navbar-toggle collapse{ /*Removes toggle bobrder*/
 +
border:none;
 +
background-color:#e8e8e8;
 +
}
 +
.navbar-inverse .navbar-toggle:hover {
 +
background-color:#47BCC2;
 +
}
 +
.navbar-inverse .navbar-toggle:focus, .navbar-inverse .navbar-toggle:hover {
 +
    background-color:#47BCC2;
 +
}
 +
.navbar-inverse .navbar-toggle:hover .icon-bar, .navbar-inverse .navbar-toggle:focus .icon-bar{
 +
background-color:#eeeeee;
 +
}
 +
/*soc classes controll css for media icons*/
 +
#soc{
 +
margin-top:-12px;
 +
 +
}
 +
/****Social Media navbar icons****/
 +
/*youtube*/
 +
#soc_1{
 +
margin-right:3px;
 +
height:50px;
 +
width:48px;
 +
}
 +
/*facebook*/
 +
#soc_2{
 +
margin-right:3px;
 +
height:50px;
 +
width:48px;
 +
}
 +
/*Twitter*/
 +
#soc_3{
 +
margin-right:3px;
 +
margin-right:18px;
 +
height:50px;
 +
width:48px;
 +
}
  
 +
/*Navbar exgem logo styling*/
 +
#logo, #logo_inverse{
 +
margin-top:-12px;
 +
vertical-align:middle;
 +
width:48px;
 +
height:48px;
 +
/*Alligns logo with toggled list on thin screens*/
 +
margin-left:13px;
 +
margin-right:-12px;
 +
}
 +
/*Logo is visable on page load by default*/
 +
#logo{
 +
display:block;
 +
}
 +
/*The inverse logo is not visable by default, but becomes visable*/
 +
/*when #logo_button is hovered over (found in jquery)*/
 +
#logo_inverse{
 +
display:none;
 +
}
  
−
<div class="column full_size">
+
/*Displays brand inline with ExGem*/
 +
.navbar-brand > img {
 +
    display: inline;
 +
}
 +
.navbar-right a{
 +
height:50px;
 +
width:48px;
 +
}
 +
#Top_link.navbar-brand{/*Top link styling*/
 +
color:#47BCC2;
 +
font-size:25px;
 +
margin-bottom:5px;
 +
opacity:0.6
 +
}
 +
#topnav{
 +
padding-bottom:0px;
 +
position:fixed;
 +
}
 +
#topnav (max-width: 767px) {
 +
display:none;
 +
}
 +
#text{/*Top link text*/
 +
color:#47BCC2;
 +
}
 +
#myNavbar{
 +
border: none;
 +
}
 +
/*sticky footer*/
 +
.wrap {
 +
  min-height: 100%;
 +
  margin-bottom: -65px;  /* equal to footer height */
 +
}
 +
.wrap:after {
 +
  content: "";
 +
  display: block;
 +
}
 +
.footer, .wrap:after {
 +
  height: 60px;
 +
  padding-top:5px;
 +
}
 +
.flare .wrap:after{
 +
height:5px;
 +
}
 +
.flare{
 +
background-color:#8cd5d9; 
 +
padding-top:3px;
 +
position:relative;
 +
bottom:0px;
 +
width:100%;
 +
}
 +
.footer{
 +
background-color:#e8e8e8;
 +
}
 +
/*Hides igm logo and team name*/
 +
#sideMenu, #top_title {display:none;}
 +
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px;}
 +
#top_title{visibility: hidden; background:#eeeeee;}
 +
#bodyContent{background:#eeeeee;}
 +
#mw-content-text{background:#eeeeee;}
 +
#HQ_page{background:none; height:0px; width:0px;}
 +
#content{background:none;}
 +
#globalWrapper{height:0px;background:#eeeeee;}
 +
/*end of hacky css :) */
 +
/*There is a <p></p> at bottom of iGEM page. In order to keep footer*/
 +
/*On bottom of page we have styled <p> tags to have no margin or border*/
 +
/*Therefore on all of our paragraphs need to id "pp" to work properly*/
 +
p{
 +
        margin:0;
 +
        padding:0;
 +
        border-style:none;
 +
}
 +
#pp{
 +
padding-top:20px;
 +
padding-right:40px;
 +
padding-left:40px;
 +
font-size:150%;
 +
}
 +
.container ul, .container ol{
 +
font-size:140%;
 +
padding-right:40px;
 +
padding-left:60px;
 +
padding-top:10px;
 +
}
 +
/*Heading styles*/
 +
h1, h2, h3, h4, h5{
 +
text-align:center;
 +
color:#339499;
 +
font-weight:500;
 +
    padding-left: 40px;
 +
    padding-right: 40px;
 +
padding-top: 40px;
 +
padding-bottom:0;
 +
}
 +
h1{font-size:340%;}
 +
h2{font-size:320%;}
 +
h3{font-size:290%;}
 +
h4{font-size:260%;}
 +
h5{font-size:220%;}
 +
h6{
 +
color:#339499;
 +
font-weight:500;
 +
font-size:200%;
 +
padding-left:40px;
 +
padding-top: 20px;
 +
padding-bottom:-10px;
 +
}
 +
/*Italics for Quotes using i tag*/
 +
q{
 +
text-align:center;
 +
color:#339499;
 +
padding-left:10%;
 +
padding-right:10%;
 +
font-size:120%;
 +
font-style: italic;
 +
}
 +
/*Styling for links*/
 +
a{
 +
color:#339499;
 +
font-weight:550;
 +
}
 +
/**page content styling starts**/
 +
body{
 +
background-color:#eeeeee;
 +
}
 +
h1{
 +
text-align:center;
 +
        border-style:none;
 +
}
 +
#Section_Link, #Section_Link:visited{
 +
display:block;
 +
margin:0 auto 10px auto;
 +
width:14px;
 +
}
 +
#Section_Link:hover{
  
 +
}
 +
#sectionGap, #sectionGap:focus, #contentTitle{
 +
min-width:100%;
 +
min-height:10vh;
 +
background:#e8e8e8;
 +
display:block;
 +
font-size:400%;
 +
text-align:center;
 +
color:#47BCC2;
 +
text-decoration:none;
 +
border-style:none none solid none;
 +
border-width:3px;
 +
border-color:#8cd5d9;
 +
}
 +
#sectionGap:hover, #sectionGap:active{
 +
color:#339499;
 +
}
 +
.container{
 +
min-width:100%;
 +
padding:0;
 +
}
 +
.div_vl{
 +
padding-top:2%;
 +
height:35vw;
 +
width:95vw;
 +
display:block;
 +
margin-left:auto;
 +
margin-right:auto;
 +
}
 +
.div_vl.backgroundimage{
 +
background-image: url('https://static.igem.org/mediawiki/2016/9/93/T--Exeter--Home_Background1.png');
 +
background-repeat:no-repeat;
 +
background-size: 100% auto;
 +
width:100%;
 +
height:88vh;
 +
}
 +
#title{
 +
style="font-family: 'Arial';
 +
color:#4e4e4e;
 +
text-align:center;
 +
margin-bottom:20px;
 +
font-size:400%;
 +
}
 +
.div_content{
 +
padding:0;
 +
min-height:100vh;
 +
width:100%;
 +
background:#eeeeee;
 +
}
  
−
<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
/*div_banner contains to links at the top of each page to link to*/
 +
/*each section of the page*/
 +
.div_banner{
 +
max-width:90vw;
 +
margin:12vh auto 5vh auto;
 +
display:block;
 +
height:8vh;
 +
}
  
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
/*Contains styling for the left and right pictures of the banner*/
 +
.subdiv_banner{
 +
height:8vh;
 +
padding:0;
 +
margin:auto;
 +
position:relative;
 +
bottom:0;
 +
right:0;
 +
}
 +
.subdiv_banner.left{
 +
float:left;
 +
display:block;
 +
}
 +
.subdiv_banner.right{
 +
float:right;
 +
display:block;
 +
}
 +
.subdiv_banner.middle{
 +
height:100%;
 +
}
 +
/*Positions the images in correct place in the divs*/
 +
.subdiv_banner.left img, .subdiv_banner.right img{
 +
width:100%;
 +
position: relative;
 +
top: 38%;
 +
-ms-transform: translateY(-50%) /* IE 9 */
 +
    -webkit-transform: translateY(-50%) /* Chrome, Safari, Opera */
 +
    transform: translateY(-50%);
 +
}
 +
.banner_link,.banner_link:visited{
 +
position: relative;
 +
height:100%;
 +
padding:0, 2px!important;
 +
color:#47BCC2;
 +
text-align:center;
 +
font-size:1.5vw;
 +
/*Max font size where "section" and "1" don't appear on*/
 +
}
 +
.banner_link span.oneline{
 +
position: relative;
 +
top: 34.5%;
 +
-ms-transform: translateY(-50%) /* IE 9 */
 +
    -webkit-transform: translateY(-50%) /* Chrome, Safari, Opera */
 +
    transform: translateY(-50%);
 +
}
 +
.banner_link span.twoline{
 +
position: relative;
 +
top: 7.5%;
 +
-ms-transform: translateY(-50%) /* IE 9 */
 +
    -webkit-transform: translateY(-50%) /* Chrome, Safari, Opera */
 +
    transform: translateY(-50%);
 +
}
 +
.banner_link:hover{
 +
text-decoration:none;
 +
color:#339499;
 +
}
 +
.link_fix{
 +
display:block;
 +
position:relative;
 +
height:1px;
 +
top:-15px;
 +
}
 +
/*Mobile and small screen css*/
 +
@media (max-width: 767px){
 +
.link_fix{
 +
display:none;
 +
}
 +
.div_vl.backgroundimage{
 +
background-image: url('#');
 +
background:#ddd;
 +
}
 +
#title{
 +
font-size:150%;
 +
}
 +
/*Makes side pictures on banner invisible on small screens*/
 +
/*as it clutters screen*/
 +
.subdiv_banner.left, .subdiv_banner.right{
 +
display:none;
 +
}
 +
}
 +
#dropdownMenu1{
 +
margin-top:-0.15vw;
 +
background:#e8e8e8;
 +
border-style:none;
 +
}
 +
@media (min-width: 767px){
 +
.dropdown-menu{
 +
margin-top:24px;
 +
left:-19px;
 +
border-radius:0px;
 +
padding:4px;
 +
}
 +
}
 +
/*Mobile and small screen css*/
 +
@media (max-width: 767px){
 +
.div_vl.backgroundimage{
 +
height:37vh;
 +
background-size: auto 200%;
 +
}
 +
.div_l{
 +
display:none;
 +
}
 +
#logo_Banner_Desktop{
 +
display:none;
 +
}
 +
#logo_Banner_Mobile{
 +
display:block;
 +
width:100%;
 +
max-width:200px;
 +
margin:auto;
 +
}
 +
#dropdownMenu1{
 +
padding:2.5% 0 2% 0;
 +
margin:-1.6% auto -1.4% 0.9%;
 +
}
 +
#links{
 +
padding:10px 0;
 +
margin-left:0.85vw;
 +
}
 +
}
 +
</style>
 +
<div id="top_page" class="link_fix"></div>
 +
<nav id="Top" class="navbar navbar-inverse navbar-static-top">
 +
  <div class="container-fluid">
 +
    <div class="navbar-header">
 +
        <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#myNavbar">
 +
          <span class="icon-bar"></span>
 +
          <span class="icon-bar"></span>
 +
          <span class="icon-bar"></span>                       
 +
</button>
 +
      <a id="logo_button" class="navbar-brand" href="https://2016.igem.org/Team:Exeter">
 +
<img id="logo" src="https://static.igem.org/mediawiki/2016/7/75/T--Exeter--Template--Logo2.png"/>
 +
<img id="logo_inverse" src="https://static.igem.org/mediawiki/2016/2/23/T--Exeter--Template_Logo2_inverse.png"/>
 +
  </a>
 +
 
 +
    </div>
 +
    <div>
 +
        <div class="collapse navbar-collapse" id="myNavbar">
 +
<ul class="nav navbar-nav">
 +
<li><div id="links" style="margin:0;" class="dropdown">
 +
  <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 +
    <span style="margin-bottom:4px;">Lab</span>
 +
    <span class="caret"></span>
 +
  </button>
 +
  <ul class="dropdown-menu" style="background:#e8e8e8;margin-left:25px;" aria-labelledby="dropdownMenu1">
 +
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Project">Lab Project</a></li>
 +
    <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Labbook">Lab Book</a></li>
  
−
</div>
+
  </ul>
 +
</div></li>
 +
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Parts">Parts</a></li>
 +
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Team">Team</a></li>
 +
<li ><a id="links" href="https://2016.igem.org/Team:Exeter/Interlab">InterLab</a></li>
 +
<li><div id="links" style="margin:0;" class="dropdown">
 +
  <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 +
    <span style="margin-bottom:4px;">Human Practices</span>
 +
    <span class="caret"></span>
 +
  </button>
 +
  <ul class="dropdown-menu" style="background:#e8e8e8;margin-left:25px;" aria-labelledby="dropdownMenu1"> 
 +
    <li><a id="links" style="margin:10px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Integrated_Practices">Integrated</a></li>
 +
<li><a id="links" style="background:none;line-height:0.7vh;margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Engagement">Public Engagement<br /><br /><br /> & Education</a></li>
 +
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Log">Log</a></li>
  
 +
  </ul>
 +
</div></li>
  
−
<div class="column full_size">
+
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Attributions">Attributions</a></li>
−
<h5>Safe Project Design</h5>
+
  
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<li><div id="links" style="margin:0;" class="dropdown">
 +
  <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 +
    <span style="margin-bottom:4px;">Awards</span>
 +
    <span class="caret"></span>
 +
  </button>
 +
  <ul class="dropdown-menu" style="background:#e8e8e8;margin-left:25px;" aria-labelledby="dropdownMenu1">
 +
 
 +
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Awards">Medals</a></li>
 +
<li><span style="margin:10px 0 30px 2px;padding:0;">Special pages</span></li>
 +
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Silver">HP Silver</a></li>
 +
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Gold">HP Gold</a></li>
 +
 
 +
  </ul>
 +
</div></li>
 +
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Model">Models</a></li>
 +
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Collaborations">Collaborations</a></li>
 +
 
 +
 
 +
 
 +
</ul>
 +
<ul class="nav navbar-nav navbar-right" >
 +
<li class="media_icon">
 +
<a  id="soc_1" href="https://www.youtube.com/channel/UC31qfG4hnm8gRHDCkrBtAiQ">
 +
<img id = "soc" src="https://static.igem.org/mediawiki/2016/2/23/You.png"/>
 +
</a>
 +
</li>
 +
    <li class="media_icon" >
 +
<a id="soc_2"href="https://www.facebook.com/ExeteriGEM2016/?ref=aymt_homepage_panel">
 +
<img id = "soc" src="https://static.igem.org/mediawiki/2016/5/51/Fb.png"/>
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<h1>Safety</h1>
 +
<h5>General Safety</h5>
 +
 
 +
<p id ="pp">As our project focuses on biosafety, investigating whether kill switches can be reliably used as biosafety devices, general safety was something we regarded as very important. Our project poses minimal threat to individuals or to the environment upon accidental release, due to us only using Risk Group 1 microorganisms. However, we will still be taking all precautions to ensure no release of biomaterials into the environment occur.</p>
 +
 
 +
<p id ="pp">Prior to beginning the laboratory work for our project, we all received a laboratory induction with Dagmara Kolak, the Mezzanine laboratory manager. As in standard with Biosafety Level 2 (BSL-2) laboratories, we signed the relevant lab safety paperwork and were guided through standard safety procedures. We were shown around the laboratory and introduced to the academics working in the laboratory, shown the locations of equipment and how to safely use it, and informed of the standard safety procedures for the Mezzanine laboratory.</p>
 +
 
 +
<p id = "pp"> All the appropriate COSHH forms were read and signed according to best laboratory practises and university policies. </p>
 +
 
 +
<h5>Laboratory Safety</h5>
 +
 
 +
<p id ="pp">The Mezzanine laboratory is, as mentioned earlier, a Category 2 laboratory. By definition, a BSL-2 laboratory “is suitable for work involving agents that pose moderate hazards to personnel and the environment” (Gla.ac.uk). A BSL-2 laboratory builds upon the pre-existing safety procedures for a BSL-1 laboratory, but has some key differences: </p>
  
 
<ul>
 
<ul>
−
<li>Choosing a non-pathogenic chassis</li>
+
<li>Laboratory personnel have training in handling in pathogenic agents, and are supervised by scientists competent in handling infectious agents.</li>
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
<li>Access to the laboratory is restricted when work is being conducted.</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
<li>All procedures involving infectious aerosols or splashes must be conducted in a biosafety cabinet (BSC) or other physical containment.</li>
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
 
</ul>
 
</ul>
  
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</div>
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<p id ="pp">In the Mezzanine laboratory, we worked alongside Biosciences researchers from different backgrounds, and were given our own bench, storage areas in the freezers and cold room. We were also given full access to the equipment in the laboratory. As the Mezzanine laboratory is a BSL-2 laboratory, we had to follow strict safety procedures to prevent release of biomaterials and/or microorganisms into the environment.</p>
  
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<div class="column half_size">
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<p id ="pp">During our project, we used <i>E. coli</i> strains BL21 DE3 and DH5α - They are classified as Risk Group 1 microorganisms (eur-lex.europa.eu, 2000). Risk Group 1 microorganisms do not cause disease in healthy adult humans, and can be used in a BSL-1 laboratory (2016.igem.uk, 2016). </p>
−
<h5>Safe Lab Work</h5>
+
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
<p id ="pp">To ensure we worked safely, and to prevent the possible release of biomaterials into the environment, we followed the procedures below:</p>
  
−
</div>
+
<ul>
 +
<li>When in the laboratory, lab coats were worn at all times. In the event of a spillage, normal clothing therefore isn’t contaminated and doesn’t risk biomaterial release.</li>
 +
<li>When in the laboratory, nitrile gloves were worn at all times.</li>
 +
<li>When leaving the laboratory, hands were always thoroughly washed.</li>
 +
<li>For a sterile environment, we always used the flow hood to reduce risk of contamination. Before items or hands were placed inside the hood, they were sanitised and wiped down with ethanol spray.</li>
 +
<li>For waste biomaterials and chemicals, all were disposed of accordingly to Mezzanine laboratory procedures to prevent contamination.</li>
 +
<li>Certain items of equipment, such as the Autoclave, were to only be used by experienced members of staff who had been trained on how to use it.</li>
 +
</ul>
  
−
<div class="column half_size">
+
<h5>References</h5>
−
<h5>Safe Shipment</h5>
+
<ol style="font-size:100%;">
 +
 
 +
<li> Gla.ac.uk. Laboratory Biosafety Level Criteria. [online] Available at: http://www.gla.ac.uk/media/media_219400_en.pdf [Accessed 9 Oct. 2016].</li>
 +
<li> ncbi.nlm.nih.gov. (1995). Rapid and Accurate Identification of <i>Escherichia coli</i> K-12 Strains. [online] Available at:  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC167724/pdf/614135.pdf [Accessed 9 Oct. 2016]. </li>
 +
<li> eur-lex.europa.eu. (2000). European Union. 2000. Directive 2000/54/EC of the European Parliament and of the Council of 18 September 2000 on the protection of workers from risks related to exposure to biological agents at work.(seventh individual directive within the meaning of Article 16(1) of Directive 89/391/EC Official Journal of the European Communities L262/21. [online] Available at: http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32000L0054&from=EN [Accessed 10 Oct. 2016].</li>
 +
<li> 2016.igem.org. (2016). Safety/Risk Groups. [online] Available at: https://2016.igem.org/Safety/Risk_Groups [Accessed 10 Oct. 2016].</li>
 +
 
 +
</ol>
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
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Latest revision as of 01:00, 20 October 2016