StefaniPjaca (Talk | contribs) |
StefaniPjaca (Talk | contribs) |
||
(12 intermediate revisions by 4 users not shown) | |||
Line 18: | Line 18: | ||
<div class="container-fluid"> | <div class="container-fluid"> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col-md-12 thumbnail" style="background-image:url(https:// | + | <div class="col-md-12 thumbnail" style="background-image:url(https://static.igem.org/mediawiki/2016/e/e1/T--DTU-Denmark--colab-header.jpg)"> <!-- EDIT style url (this should lead to an image) --> |
<div class="caption"> | <div class="caption"> | ||
<div class="col-md-5 col-sm-5 col-xs-12 title"> <!-- the approximate max number of characters ~ 400 --> <!-- EDIT --> | <div class="col-md-5 col-sm-5 col-xs-12 title"> <!-- the approximate max number of characters ~ 400 --> <!-- EDIT --> | ||
− | <h1>Collaborations<p class="lead">A great iGEM project is so much more than getting an idea and conducting a project. During our time in the iGEM competition we have reached out in order to share our expertise, help other teams in their projects | + | <h1>Collaborations<p class="lead">A great iGEM project is so much more than getting an idea and conducting a project. During our time in the iGEM competition we have reached out in order to share our expertise, help other teams in their projects and have also received a helping hand from other iGEM teams and our great sponsors!</p></h1> |
</div> | </div> | ||
<div class="col-md-2 col-sm-2 hidden-xs space"></div> | <div class="col-md-2 col-sm-2 hidden-xs space"></div> | ||
Line 50: | Line 50: | ||
<small>Helen Keller<cite title="Source Title"></cite></small> | <small>Helen Keller<cite title="Source Title"></cite></small> | ||
</blockquote> | </blockquote> | ||
− | <h3 class="h3"> | + | <h3 class="h3">BioBrick Tutorial with SDU-Denmark and UNIK-Copenhagen</h3> |
<p> | <p> | ||
The DTU BioBuilders 2016 hosted a BioBrick workshop during the Spring (May 6th - May 8th) for the other Danish iGEM teams; SDU-Denmark & UNIK-Copenhagen. | The DTU BioBuilders 2016 hosted a BioBrick workshop during the Spring (May 6th - May 8th) for the other Danish iGEM teams; SDU-Denmark & UNIK-Copenhagen. | ||
Line 56: | Line 56: | ||
<p> | <p> | ||
− | The scope of the workshop was to introduce all three teams to the BioBrick standard, get a feel of working with it in the laboratory and get to know the other Danish iGEM teams. During the weekend we had planned different lectures that all contributed to a better understanding of | + | The scope of the workshop was to introduce all three teams to the BioBrick standard, get a feel of working with it in the laboratory and get to know the other Danish iGEM teams. During the weekend we had planned different lectures that all contributed to a better understanding of BioBricks and iGEM in general. |
</p> | </p> | ||
<p> | <p> | ||
− | + | Our supervisor, Christopher Workman, gave a judging perspective on the iGEM competition and we discussed the gold medal requirements. iGEM alumni Pernille Neve Myers and Viktor Hesselberg-Thomsen from DTU BioBuilders 2015 held a lecture about BioBricks and 3A Assembly along with exciting exercises in the laboratory. The tutorial also had another aspect, namely the design and development of the wiki. Here, we had this years SDU-Denmark team give us a presentation of general advices for a good wiki and alumnus Mads Anderson introduced the “Wiki-Wizard”. | |
</p> | </p> | ||
Line 66: | Line 66: | ||
</p> | </p> | ||
<p> | <p> | ||
− | The weekend was a great success and gave the Danish teams a great foundation for collaboration and fun during other meetups and during our projects. | + | The weekend was a great success and gave the Danish teams a great foundation for collaboration and fun during other meetups and during our projects. In order to assure that next year's Biobuilders could make it even better, we asked everyone for feedback and had a follow up presentation: |
+ | <ul> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2016/b/b9/T--DTU-Denmark--Tutorial-followup.pdf">Summary of the feedback</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2016/b/b5/T--DTU-Denmark--tutorial-externalreplies.pdf">Feedback from SDU-Denmark and UNIK-Copenhagen</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2016/0/04/T--DTU-Denmark--tutorial-internalreplies.pdf">Feedback from DTU BioBuilders</a></li> | ||
+ | </ul> | ||
</p> | </p> | ||
+ | |||
+ | <div class = "col-md-6"> | ||
+ | <figure class="figure"> | ||
+ | <img id="BBlab" class="enlarge img-responsive figure-img" src="https://static.igem.org/mediawiki/2016/8/8e/T--DTU-Denmark--BBlab.jpg" alt="DESCRIPTION"> | ||
+ | <figcaption class="figure-caption">Laboratory shot at the BioBrick Tutorial</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | |||
+ | <!-- The Modal with same picture--> | ||
+ | <div id="BBlabModal" class="modal"> | ||
+ | <span class="close BBlab">×</span> | ||
+ | <img class="modal-content" id="BBlabImg"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class = "col-md-6"> | ||
+ | <figure class="figure"> | ||
+ | <img id="BBout" class="enlarge img-responsive figure-img" src="https://static.igem.org/mediawiki/2016/f/f9/T--DTU-Denmark--BBout.jpg" alt="DESCRIPTION"> | ||
+ | <figcaption class="figure-caption">Group shot at the BioBrick Tutorial with SDU-Denmark and UNIK-Copenhagen</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | |||
+ | <!-- The Modal with same picture--> | ||
+ | <div id="BBoutModal" class="modal"> | ||
+ | <span class="close BBout">×</span> | ||
+ | <img class="modal-content" id="BBoutImg"> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
<h3 class="h3">Uppsala University</h3> | <h3 class="h3">Uppsala University</h3> | ||
<p> | <p> | ||
− | + | In July, we received a request for help from team Uppsala. Team Uppsala wanted to test their microfluidic devices in both prokaryotic and eukaryotic organisms. However, the team did not have a yeast lab at their university and lacked some necessary components in order to work with yeast thus we sent two <i>Saccharomyces cerevisiae</i> strains, W1588-4A and W1588-4C, in order to help the Uppsala team with the testing of their method in a eukaryotic organism. | |
</p> | </p> | ||
Line 93: | Line 127: | ||
<h3 class="h3">Uppsala</h3> | <h3 class="h3">Uppsala</h3> | ||
<p> | <p> | ||
− | We tried to insert chromoproteins into the chlorampenicol backbone pSB1C3, but without success.Therefore, we contacted team Uppsala in order to get some help. Since the team had chromoproteins in stock from prior iGEM Uppsala teams, they helped us moving one of the chromoproteins from the | + | We tried to insert chromoproteins into the chlorampenicol backbone pSB1C3, but without success. Therefore, we contacted team Uppsala in order to get some help. Since the team had chromoproteins in stock from prior iGEM Uppsala teams, they helped us moving one of the chromoproteins from the pSB1A3 backbone into the pSB1C3 backbone. |
</p> | </p> | ||
<h3 class="h3">SDU-Denmark</h3> | <h3 class="h3">SDU-Denmark</h3> | ||
<p> | <p> | ||
− | We also contacted SDU-Denmark about expression of chromoproteins in | + | We also contacted SDU-Denmark about expression of chromoproteins in pSB1C3. SDU-Denmark gave us some advice and told us to use another promoter in order to express the chromoproteins. |
Line 107: | Line 141: | ||
<h3 class="h3">Nordic iGEM Conference</h3> | <h3 class="h3">Nordic iGEM Conference</h3> | ||
<p> | <p> | ||
− | In June, DTU BioBuilders went to Nordic iGEM Conference (NiC) in Stockholm hosted by team Stockholm. During the weekend, DTU BioBuilders connected and shared their projects with the other Nordic teams. DTU BioBuilders presented their project at a mini-Jamboree, where the requirements set by iGEM Headquarters were in focus. The mini-Jamboree gave DTU BioBuilders the opportunity to get a feel of the big iGEM competition in Boston and refine their project. | + | In June, DTU BioBuilders went to the Nordic iGEM Conference (NiC) in Stockholm hosted by team Stockholm. During the weekend, DTU BioBuilders connected and shared their projects with the other Nordic teams. DTU BioBuilders presented their project at a mini-Jamboree, where the requirements set by iGEM Headquarters were in focus. The mini-Jamboree gave DTU BioBuilders the opportunity to get a feel of the big iGEM competition in Boston and refine their project. |
</p> | </p> | ||
<p> | <p> | ||
− | Besides the mini-Jamboree and two workshops, Ethics and Speculative Design of Synthetic Biology, there was also time for | + | Besides the mini-Jamboree and two workshops, Ethics and Speculative Design of Synthetic Biology, there was also time for barbeques and other social activities to get to know the other teams. |
</p> | </p> | ||
Line 119: | Line 153: | ||
<h3 class="h3">The European iGEM Experience</h3> | <h3 class="h3">The European iGEM Experience</h3> | ||
− | <p> | + | <div class = "col-md-6"><p> |
In July, iGEM EVRY and iGEM IONIS hosted a big gathering in Paris for many of the European iGEM teams. | In July, iGEM EVRY and iGEM IONIS hosted a big gathering in Paris for many of the European iGEM teams. | ||
</p> | </p> | ||
Line 125: | Line 159: | ||
<p> | <p> | ||
− | DTU BioBuilders attended this big gathering and presented and opposed their project to the other teams. It was a great occasion to share ideas and explore new possibilities about our project. Furthermore, the team had two interesting talks about synthetic biology, where they learned about challenges, risks and the | + | DTU BioBuilders attended this big gathering and presented and opposed their project to the other teams. It was a great occasion to share ideas and explore new possibilities about our project. Furthermore, the team had two interesting talks about synthetic biology, where they learned about challenges, risks and the economical aspect of synthetic biology. |
− | </p> | + | </p></div> |
+ | |||
+ | <div class="col-md-6"> | ||
+ | <figure class="figure"> | ||
+ | <img id="Parigrou" class="enlarge img-responsive figure-img" src="https://static.igem.org/mediawiki/2016/e/eb/T--DTU-Denmark--Paris-group.jpg" alt="DESCRIPTION"> | ||
+ | <figcaption class="figure-caption"> Four of the DTU BioBuilders at the European iGEM Experience 2016 | ||
+ | together with Randy Rettberg, the president of the iGEM Foundation.</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | |||
+ | <!-- The Modal with same picture--> | ||
+ | <div id="ParigrouModal" class="modal"> | ||
+ | <span class="close Parigrou">×</span> | ||
+ | <img class="modal-content" id="ParigrouImg"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
</div> | </div> | ||
Latest revision as of 01:18, 20 October 2016
Helping other teams
“Alone we can do so little; together we can do so much”
Helen Keller
BioBrick Tutorial with SDU-Denmark and UNIK-Copenhagen
The DTU BioBuilders 2016 hosted a BioBrick workshop during the Spring (May 6th - May 8th) for the other Danish iGEM teams; SDU-Denmark & UNIK-Copenhagen.
The scope of the workshop was to introduce all three teams to the BioBrick standard, get a feel of working with it in the laboratory and get to know the other Danish iGEM teams. During the weekend we had planned different lectures that all contributed to a better understanding of BioBricks and iGEM in general.
Our supervisor, Christopher Workman, gave a judging perspective on the iGEM competition and we discussed the gold medal requirements. iGEM alumni Pernille Neve Myers and Viktor Hesselberg-Thomsen from DTU BioBuilders 2015 held a lecture about BioBricks and 3A Assembly along with exciting exercises in the laboratory. The tutorial also had another aspect, namely the design and development of the wiki. Here, we had this years SDU-Denmark team give us a presentation of general advices for a good wiki and alumnus Mads Anderson introduced the “Wiki-Wizard”.
Beside having an educational weekend, we had a lot of fun and got to know the other iGEM teams in Denmark!
The weekend was a great success and gave the Danish teams a great foundation for collaboration and fun during other meetups and during our projects. In order to assure that next year's Biobuilders could make it even better, we asked everyone for feedback and had a follow up presentation:
Uppsala University
In July, we received a request for help from team Uppsala. Team Uppsala wanted to test their microfluidic devices in both prokaryotic and eukaryotic organisms. However, the team did not have a yeast lab at their university and lacked some necessary components in order to work with yeast thus we sent two Saccharomyces cerevisiae strains, W1588-4A and W1588-4C, in order to help the Uppsala team with the testing of their method in a eukaryotic organism.
Furthermore, we sent the team two shuttle vectors, pRS415 and pRS423, a high copy-number and a low copy-number vector, respectively, which the team has used to perform their tests in yeast.
SDU-Denmark and UNIK-Copenhagen
In August, the bioinformaticians from DTU BioBuilders, SDU-Denmark and UNIK-Copenhagen arranged a meeting for discussing ideas on how to apply mathematical modelling to each other’s projects.
SDU-Denmark and UNIK-Copenhagen explained their issues, and the DTU BioBuilders suggested suitable models based on systems of differential equations, that were well-received by both teams.
Furthermore, the DTU BioBuilders explained the genome-scale model (GSM) concept and received reasonable questions and suggestions from the other teams.
Getting help ourselves
Uppsala
We tried to insert chromoproteins into the chlorampenicol backbone pSB1C3, but without success. Therefore, we contacted team Uppsala in order to get some help. Since the team had chromoproteins in stock from prior iGEM Uppsala teams, they helped us moving one of the chromoproteins from the pSB1A3 backbone into the pSB1C3 backbone.
SDU-Denmark
We also contacted SDU-Denmark about expression of chromoproteins in pSB1C3. SDU-Denmark gave us some advice and told us to use another promoter in order to express the chromoproteins.
Meetups
Nordic iGEM Conference
In June, DTU BioBuilders went to the Nordic iGEM Conference (NiC) in Stockholm hosted by team Stockholm. During the weekend, DTU BioBuilders connected and shared their projects with the other Nordic teams. DTU BioBuilders presented their project at a mini-Jamboree, where the requirements set by iGEM Headquarters were in focus. The mini-Jamboree gave DTU BioBuilders the opportunity to get a feel of the big iGEM competition in Boston and refine their project.
Besides the mini-Jamboree and two workshops, Ethics and Speculative Design of Synthetic Biology, there was also time for barbeques and other social activities to get to know the other teams.
Human Practice Workshop
UNIK-Copenhagen hosted a Human Practice workshop in August where the DTU BioBuilders attended together with SDU-Denmark and Chalmers-Gothenburg. The workshop was very useful and a great success, and DTU BioBuilders were involved in patent reflections, public speaking, ethics, philosophy, and human practices in general.
The European iGEM Experience
In July, iGEM EVRY and iGEM IONIS hosted a big gathering in Paris for many of the European iGEM teams.
DTU BioBuilders attended this big gathering and presented and opposed their project to the other teams. It was a great occasion to share ideas and explore new possibilities about our project. Furthermore, the team had two interesting talks about synthetic biology, where they learned about challenges, risks and the economical aspect of synthetic biology.