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| background-color: #ffffff; | | background-color: #ffffff; |
| } | | } |
− | #main-slider-wrapper{ | + | |
| + | #main-slider-wrapper { |
| margin: 0 auto; | | margin: 0 auto; |
| + | } |
| + | #main-slider{ |
| + | margin-bottom: 100px; |
| } | | } |
| </style> | | </style> |
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| <body> | | <body> |
| <header class="header"> | | <header class="header"> |
| + | <div class="navbar-logo"> |
| + | <a class="navbar-brand" href="https://igem.org/Main_Page"> |
| + | <img alt="Brand" src="https://2016.igem.org/wiki/skins/Igem/images/IGEM_white_letters.png"> |
| + | </a> |
| + | </div> |
| + | <div class="navbar-logo"> |
| + | <a class="navbar-brand" href="https://2016.igem.org/Team:HUST-China"> |
| + | <img alt="Brand" src="https://static.igem.org/mediawiki/2016/0/01/T--HUST-China--logo.png" style="left:100px;width:40px;"> |
| + | </a> |
| + | </div> |
| <nav class="navbar navbar-default"> | | <nav class="navbar navbar-default"> |
| <div class="container"> | | <div class="container"> |
− | <span class="menu"> Menu</span> | + | <span class="menu">Menu</span> |
| <div class="banner-top"> | | <div class="banner-top"> |
| <ul class="nav banner-nav"> | | <ul class="nav banner-nav"> |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown1"><a class="down-scroll" href="https://2016.igem.org/Team:HUST-China/Model">MODELING</a> | + | <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a> |
| <ul class="dropdown2"> | | <ul class="dropdown2"> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li> |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown1"><a class="down-scroll" href="">PARTS</a> | + | <li class="dropdown1"><a class="down-scroll" href="#">PARTS</a> |
| <ul class="dropdown2"> | | <ul class="dropdown2"> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li> |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown1"><a class="down-scroll" href="">TEAM</a> | + | <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a> |
| <ul class="dropdown2"> | | <ul class="dropdown2"> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Team">Team Roster</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Team">Team Roster</a></li> |
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| <div class="container-fluid"> | | <div class="container-fluid"> |
| <div class="text-content" id="text-content"> | | <div class="text-content" id="text-content"> |
− | <div class="ref"></div> | + | <div class="ref"> |
| <section class="liquid-slider" id="main-slider"> | | <section class="liquid-slider" id="main-slider"> |
| <div> | | <div> |
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| <tr> | | <tr> |
| <td>1</td> | | <td>1</td> |
− | <td>CII 1</td> | + | <td>CII</td> |
| </tr> | | </tr> |
− | <tr>
| |
− | <td>2</td>
| |
− | <td>CII 2</td>
| |
− | </tr>
| |
− |
| |
| </table> | | </table> |
| </p> | | </p> |
| <img src="https://static.igem.org/mediawiki/2016/f/f8/T--HUST-China--notebook-CII-fig01.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/f/f8/T--HUST-China--notebook-CII-fig01.png" alt=""> |
| </div> | | </div> |
| + | |
| <div> | | <div> |
| <h2 class="title">CIII</h2> | | <h2 class="title">CIII</h2> |
Line 342: |
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| <h6>7.14</h6> | | <h6>7.14</h6> |
| <h6>PCR</h6> | | <h6>PCR</h6> |
| + | <h6>Yufeng Mo</h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Total</td> |
| + | </tr> |
| + | <tr> |
| + | <td>CIII 1 |
| + | <td>50 μl</td> |
| + | </tr> |
| + | </table> |
| <h6>7.15</h6> | | <h6>7.15</h6> |
| <h6>Restriction Enzyme Digestion </h6> | | <h6>Restriction Enzyme Digestion </h6> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>plasmid psb1c3 2 | + | <td>CIII |
| <td>XbaI and SpeI</td> | | <td>XbaI and SpeI</td> |
| </tr> | | </tr> |
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| <img src="https://static.igem.org/mediawiki/2016/8/8c/T--HUST-China--notebook-CIII-fig01.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/8/8c/T--HUST-China--notebook-CIII-fig01.png" alt=""> |
| <br> | | <br> |
| + | <h6>7.15</h6> |
| + | <h6>Dephosphorylation</h6> |
| + | <h6>Yufeng Mo</h6> |
| <h6>7.17</h6> | | <h6>7.17</h6> |
| <h6>DNA ligation&transformation</h6> | | <h6>DNA ligation&transformation</h6> |
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| <br> | | <br> |
| <h6>7.18</h6> | | <h6>7.18</h6> |
− | <h6>PCR</h6> | + | <h6>Colony PCR</h6> |
| <h6>Yufeng Mo</h6> | | <h6>Yufeng Mo</h6> |
| <table border="1"> | | <table border="1"> |
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| <td>Name | | <td>Name |
| <td>Total</td> | | <td>Total</td> |
| + | </tr> |
| + | <tr> |
| + | <td>RBS-CIII |
| + | <td>50 μl</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
Line 534: |
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| <h6>8.10</h6> | | <h6>8.10</h6> |
| <h6>DNA ligation&transformation</h6> | | <h6>DNA ligation&transformation</h6> |
− | <h6>Zhenyi Guo</h6> | + | <h6>Ziyi Jiang</h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Backbone |
| + | <td>Strain |
| + | <td>Medium</td> |
| + | </tr> |
| + | <tr> |
| + | <td>RBS-CIII |
| + | <td>psb1c3(XbaI and PstI cut) |
| + | <td> DH5α |
| + | <td>LB(chl added) </td> |
| + | </tr> |
| + | </table> |
| + | <br> |
| + | <h6>8.11</h6> |
| + | <h6>Colony PCR</h6> |
| + | <h6>Ziyi Jiang</h6> |
| <table border="1"> | | <table border="1"> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td>2 | | <td>2 |
− | <td>CIII-RBS</td> | + | <td>RBS-CIII</td> |
| </tr> | | </tr> |
| </table> | | </table> |
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| <h6>8.19</h6> | | <h6>8.19</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6>
| |
| <h6>Ziyi Jiang</h6> | | <h6>Ziyi Jiang</h6> |
| <table border="1"> | | <table border="1"> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>RBS-CII | + | <td>RBS-CII-psb1c3 |
| <td>EcoRI and XbaI</td> | | <td>EcoRI and XbaI</td> |
| </tr> | | </tr> |
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| <tr> | | <tr> |
| <td>RBS-CII | | <td>RBS-CII |
− | <td>RBS-CII-psb1c3(EcoRI and SpeI cut) | + | <td>RBS-CII-psb1c3(EcoRI and XbaI cut) |
| <td> DH5α | | <td> DH5α |
| <td>LB(chl added) </td> | | <td>LB(chl added) </td> |
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| <h6>9.15</h6> | | <h6>9.15</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6>
| |
| <h6>Zhi Zeng</h6> | | <h6>Zhi Zeng</h6> |
| <table border="1"> | | <table border="1"> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>GFP-F 1 | + | <td>GFP-First |
| <td>50 μl</td> | | <td>50 μl</td> |
| </tr> | | </tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>GFP-X-F | + | <td>pRM-RBS-GFP-LAVtag |
| <td>30 μl</td> | | <td>30 μl</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>GFP-X-F | + | <td>pRM-RBS-GFP-LAVtag |
| <td>30 μl</td> | | <td>30 μl</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>GFP-X-F | + | <td>pRM-RBS-GFP-LAVtag |
| <td>30 μl</td> | | <td>30 μl</td> |
| </tr> | | </tr> |
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| <h6>7.19</h6> | | <h6>7.19</h6> |
| <h6>Restriction Enzyme Digestion</h6> | | <h6>Restriction Enzyme Digestion</h6> |
| + | <h6>Xiaoyang Li</h6> |
| <table border="1"> | | <table border="1"> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>pRM-RBS-GFP | + | <td>pRM-RBS-GFP-LAVtag |
| <td> psb1c3(EcoRI and SpeI cut) | | <td> psb1c3(EcoRI and SpeI cut) |
| <td> DH5α | | <td> DH5α |
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| <p> | | <p> |
| <h6>8.21</h6> | | <h6>8.21</h6> |
| + | <h6>Restriction Enzyme Digestion</h6> |
| <h6>Yufeng Mo</h6> | | <h6>Yufeng Mo</h6> |
| <table border="1"> | | <table border="1"> |
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| <tr> | | <tr> |
| <td>pRM-RBS-GFP-LVAtag | | <td>pRM-RBS-GFP-LVAtag |
− | <td>XbaI and PstI</td> | + | <td>EcoRI and PstI</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td> Duet-pet | + | <td> pETDuet-1 |
− | <td> XbaI and PstI </td> | + | <td> EcoRI and PstI </td> |
| </tr> | | </tr> |
| </table> | | </table> |
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| <tr> | | <tr> |
| <td>pRM-RBS-GFP-LVAtag | | <td>pRM-RBS-GFP-LVAtag |
− | <td> pet-Duet-1(XbaI and PstI cut) | + | <td> pETDuet-1(EcoRI and PstI cut) |
| <td> DH5α | | <td> DH5α |
| <td>LB(Amp added) </td> | | <td>LB(Amp added) </td> |
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| <tr> | | <tr> |
| <td>1 | | <td>1 |
− | <td>pRM-RBS-GFP-LVAtag-Duet-pet 1</td> | + | <td>pRM-RBS-GFP-LVAtag-pETDuet 1</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>3 | | <td>3 |
− | <td> pRM-RBS-GFP-LVAtag-Duet-pet 3</td> | + | <td> pRM-RBS-GFP-LVAtag-pETDuet 3</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>4 | | <td>4 |
− | <td> pRM-RBS-GFP-LVAtag-Duet-pet 4</td> | + | <td> pRM-RBS-GFP-LVAtag-pETDuet 4</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>5 | | <td>5 |
− | <td> pRM-RBS-GFP-LVAtag-Duet-pet 5</td> | + | <td> pRM-RBS-GFP-LVAtag-pETDuet 5</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>6 | | <td>6 |
− | <td> pRM-RBS-GFP-LVAtag-Duet-pet 6</td> | + | <td> pRM-RBS-GFP-LVAtag-pETDuet 6</td> |
| </tr> | | </tr> |
| </table> | | </table> |
| <br> | | <br> |
| <img src="https://static.igem.org/mediawiki/2016/6/66/T--HUST-China--notebook-pRM-RBS-GFP-LVAtag-fig03.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/6/66/T--HUST-China--notebook-pRM-RBS-GFP-LVAtag-fig03.png" alt=""> |
− | </p>
| |
− | <p>
| |
− | <h6>7.24</h6>
| |
− | <h6>Colony PCR</h6>
| |
− | <h6>Xiaoyang Li</h6>
| |
− | <table border="1">
| |
− | <tr>
| |
− | <td>Number
| |
− | <td>Name</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>1
| |
− | <td> pRM-RBS-GFP-LVAtag</td>
| |
− | </tr>
| |
− | </table>
| |
− | <br>
| |
− | <img src="https://static.igem.org/mediawiki/2016/e/ec/T--HUST-China--notebook-pRM-RBS-GFP-LVAtag-fig02.png" alt="">
| |
| </p> | | </p> |
| </div> | | </div> |
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| <td>Name | | <td>Name |
| <td>Total</td> | | <td>Total</td> |
| + | </tr> |
| + | <tr> |
| + | <td>pR-RBS-GFP-tag |
| + | <td>50 μl</td> |
| + | </tr> |
| + | <tr> |
| + | <td>pR-RBS-GFP-tag |
| + | <td>50 μl</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </p> | | </p> |
| <p> | | <p> |
| + | <h6>8.2</h6> |
| <h6>Restriction Enzyme Digestion</h6> | | <h6>Restriction Enzyme Digestion</h6> |
| <h6>Zhenyi Guo</h6> | | <h6>Zhenyi Guo</h6> |
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| </p> | | </p> |
| <p> | | <p> |
| + | <h6>8.2</h6> |
| <h6>DNA ligation&Transformation</h6> | | <h6>DNA ligation&Transformation</h6> |
| <h6>Zhenyi Guo</h6> | | <h6>Zhenyi Guo</h6> |
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| <h6>8.4</h6> | | <h6>8.4</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6> | + | <h6>Zhenyi Guo</h6> |
| <table border="1"> | | <table border="1"> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td>pR-RBS-GFP-tag | | <td>pR-RBS-GFP-tag |
− | <td> petDuet-1(XbaI and PstI cut) | + | <td> pETDuet-1(EcoRI and PstI cut) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Amp added) </td> | | <td>LB(Amp added) </td> |
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| <tr> | | <tr> |
| <td>4 | | <td>4 |
− | <td> pR-RBS-GFP-tag-petDuet-1 4</td> | + | <td> pR-RBS-GFP-tag-pETDuet-1 4</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>6 | | <td>6 |
− | <td> pR-RBS-GFP-tag-petDuet-1 6</td> | + | <td> pR-RBS-GFP-tag-pETDuet-1 6</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>7 | | <td>7 |
− | <td> pR-RBS-GFP-tag-petDuet-1 7</td> | + | <td> pR-RBS-GFP-tag-pETDuet-1 7</td> |
| </tr> | | </tr> |
| </table> | | </table> |
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| <div> | | <div> |
| <h2 class="title">SnRK2.2</h2> | | <h2 class="title">SnRK2.2</h2> |
− | <p>
| |
− | <h6>8.25</h6>
| |
− | <h6>Site-mutated PCR</h6>
| |
− | <h6>Fengyi Li</h6>
| |
− | <table border="1">
| |
− | <tr>
| |
− | <td>Name
| |
− | <td>Total</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>SnRK2.2
| |
− | <td>40 μl</td>
| |
− | </tr>
| |
− | </table>
| |
− | <img src="https://static.igem.org/mediawiki/2016/4/4b/T--HUST-China--notebook-SnRK2.2-fig01.png" alt="">
| |
− | </p>
| |
| <p> | | <p> |
| <h6>9.10</h6> | | <h6>9.10</h6> |
| <h6>PCR</h6> | | <h6>PCR</h6> |
− | <h6>more details, protocol</h6>
| |
| <h6>Yufeng Mo</h6> | | <h6>Yufeng Mo</h6> |
| <table border="1"> | | <table border="1"> |
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| <h6>9.12</h6> | | <h6>9.12</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6>
| |
| <h6>Yufeng Mo</h6> | | <h6>Yufeng Mo</h6> |
| <table border="1"> | | <table border="1"> |
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Line 1,233: |
| <h6>9.10</h6> | | <h6>9.10</h6> |
| <h6>PCR</h6> | | <h6>PCR</h6> |
− | <h6>more details,protocol</h6>
| |
| <h6>Yufeng Mo</h6> | | <h6>Yufeng Mo</h6> |
| <table border="1"> | | <table border="1"> |
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Line 1,400: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>prd29a | + | <td>prd29a 1 |
| + | <td>50 μl</td> |
| + | </tr> |
| + | <tr> |
| + | <td>prd29a 2 |
| + | <td>50 μl</td> |
| + | </tr> |
| + | <tr> |
| + | <td>prd29a 3 |
| + | <td>50 μl</td> |
| + | </tr> |
| + | <tr> |
| + | <td>prd29a 4 |
| <td>50 μl</td> | | <td>50 μl</td> |
| </tr> | | </tr> |
Line 1,405: |
Line 1,437: |
| <h6>9.28</h6> | | <h6>9.28</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6>
| |
− | <h6>Zhenyi Guo</h6>
| |
− | <table border="1">
| |
− | <tr>
| |
− | <td>Name
| |
− | <td>Enzyme</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>prd29a
| |
− | <td>XbaI and PstI</td>
| |
− | </tr>
| |
− | </table>
| |
− | <img src="https://static.igem.org/mediawiki/2016/0/05/T--HUST-China--notebook-prd29a-fig02.png" alt="">
| |
− | </p>
| |
− | <p>
| |
− | <h6>9.27</h6>
| |
− | <h6>Restriction Enzyme Digestion</h6>
| |
| <h6>Zhenyi Guo</h6> | | <h6>Zhenyi Guo</h6> |
| <table border="1"> | | <table border="1"> |
Line 1,479: |
Line 1,494: |
| <tr> | | <tr> |
| <td> CI-TTADH1-pR-RBS-GFP-LVAtag | | <td> CI-TTADH1-pR-RBS-GFP-LVAtag |
− | <td>Duet-pet(T7) | + | <td>Duet-pet(EcoRI and PstI cut) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Amp added) </td> | | <td>LB(Amp added) </td> |
Line 1,485: |
Line 1,500: |
| <tr> | | <tr> |
| <td> CII-TTADH1-pRE-RBS-GFP-LVAtag | | <td> CII-TTADH1-pRE-RBS-GFP-LVAtag |
− | <td>Duet-pet(T7) | + | <td>Duet-pet(EcoRI and PstI cut) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Amp added) </td> | | <td>LB(Amp added) </td> |
Line 1,494: |
Line 1,509: |
| <h6>8.18</h6> | | <h6>8.18</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6>
| |
| <h6>Lin Di</h6> | | <h6>Lin Di</h6> |
| <table border="1"> | | <table border="1"> |
Line 1,519: |
Line 1,533: |
| </div> | | </div> |
| <div> | | <div> |
− | <h2 class="title">Cro-TT-pRM-RBS-GFP-LVAtag-PSB1C3</h2> | + | <h2 class="title">cro-TT-pRM-RBS-GFP-LVAtag-PSB1C3</h2> |
| <p> | | <p> |
| <h6>8.14</h6> | | <h6>8.14</h6> |
Line 1,530: |
Line 1,544: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>Cro | + | <td>cro |
| <td>80 μl</td> | | <td>80 μl</td> |
| </tr> | | </tr> |
Line 1,547: |
Line 1,561: |
| <td> pRM-RBS-GFP-LVAtag-psb1c3 | | <td> pRM-RBS-GFP-LVAtag-psb1c3 |
| <td>EcoRI and XbaI </td> | | <td>EcoRI and XbaI </td> |
− | </tr>
| |
− | <tr>
| |
− | <td>psb1c3
| |
− | <td>XbaI and PstI </td>
| |
| </tr> | | </tr> |
| </table><img src="https://static.igem.org/mediawiki/2016/b/b5/T--HUST-China--notebook-Cro-T-P-R-G-tag-fig02.png" alt=""> | | </table><img src="https://static.igem.org/mediawiki/2016/b/b5/T--HUST-China--notebook-Cro-T-P-R-G-tag-fig02.png" alt=""> |
Line 1,582: |
Line 1,592: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>Cro-TT-pRM-RBS-GFP-LVAtag-PSB1C3 (ligate by Infusion) | + | <td>cro-TT-pRM-RBS-GFP-LVAtag-PSB1C3 (ligated by In-fusion) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Chl added)</td> | | <td>LB(Chl added)</td> |
Line 1,598: |
Line 1,608: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td> Cro-TT-pRM-RBS-GFP-LVAtag-psb1c3 | + | <td> cro-TT-pRM-RBS-GFP-LVAtag-psb1c3 |
| <td>20uL</td> | | <td>20uL</td> |
| </tr> | | </tr> |
Line 1,614: |
Line 1,624: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td> Duet-pet | + | <td> PETDuet-1 |
| <td>EcoRI and PstI </td> | | <td>EcoRI and PstI </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>Cro-TT-pRM-RBS-GFP-LVAtag | + | <td>cro-TT-pRM-RBS-GFP-LVAtag |
| <td>EcoRI and PstI</td> | | <td>EcoRI and PstI</td> |
| </tr> | | </tr> |
Line 1,635: |
Line 1,645: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td> Cro-TT-pRM-RBS-GFP-LVAtag | + | <td> cro-TT-pRM-RBS-GFP-LVAtag |
− | <td> Duet-pet(T7) | + | <td> pETDuet-1(EcoRI and PstI cut) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Amp added) </td> | | <td>LB(Amp added) </td> |
Line 1,664: |
Line 1,674: |
| </table> | | </table> |
| <img src="https://static.igem.org/mediawiki/2016/2/2b/T--HUST-China--notebook-PP-TT-fig01.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/2/2b/T--HUST-China--notebook-PP-TT-fig01.png" alt=""> |
| + | <h6>DNA ligation&Transformation</h6> |
| + | <h6>Ziyi Jiang</h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Backbone |
| + | <td>Strain |
| + | <td>Medium</td> |
| + | </tr> |
| + | <tr> |
| + | <td> PP2CA |
| + | <td>TTADH1-psb1c3(EcoRI and XbaI cut) |
| + | <td>DH5α |
| + | <td>LB(Chl added) </td> |
| + | </tr> |
| </p> | | </p> |
| <p> | | <p> |
| <h6>8.16</h6> | | <h6>8.16</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6>
| |
| <h6>Ziyi Jiang</h6> | | <h6>Ziyi Jiang</h6> |
| <table border="1"> | | <table border="1"> |
Line 1,755: |
Line 1,779: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td> PP2CA-TTADH1-pCyc-psb1c3 (ligate by In-Fusion) | + | <td> PP2CA-TTADH1-pCyc-psb1c3 (ligated by In-Fusion) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Chl added) </td> | | <td>LB(Chl added) </td> |
Line 1,810: |
Line 1,834: |
| </tr> | | </tr> |
| </table> | | </table> |
− | <br><img src="https://static.igem.org/mediawiki/2016/4/41/T--HUST-China--notebook-K-S-T-P-fig02.png" alt=""> | + | <br><img src="https://static.igem.org/mediawiki/2016/5/50/T--HUST-China--notebook-K-S-T-P-fig03.png" alt=""> |
| </p> | | </p> |
| <p> | | <p> |
Line 1,828: |
Line 1,852: |
| </tr> | | </tr> |
| </table> | | </table> |
− | <br><img src="https://static.igem.org/mediawiki/2016/3/3b/T--HUST-China--notebook-K-S-T-P-fig01.png" alt="">
| |
| </p> | | </p> |
| <p> | | <p> |
Line 1,841: |
Line 1,864: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td> Kozak-Snrk2.2-TTADH1-pCyc-psb1c3(ligate by In-Fusion) | + | <td> Kozak-Snrk2.2-TTADH1-pCyc-psb1c3(ligated by In-Fusion) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Chl added) </td> | | <td>LB(Chl added) </td> |
Line 1,909: |
Line 1,932: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td> Placm-pRE-RBS-Cro-RBS-CII-TT-psb1c3(ligate by In-Fusion) | + | <td> Placm-pRE-RBS-Cro-RBS-CII-TT-psb1c3(ligated by In-Fusion) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Chl added) </td> | | <td>LB(Chl added) </td> |
Line 1,945: |
Line 1,968: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>Patp2-RBS-CI-TT | + | <td>Patp2-RBS-CI-TT 1 |
| + | <td>50uL</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Patp2-RBS-CI-TT 2 |
| <td>50uL</td> | | <td>50uL</td> |
| </tr> | | </tr> |
Line 1,967: |
Line 1,994: |
| </tr> | | </tr> |
| </table> | | </table> |
− | <br><img src="https://static.igem.org/mediawiki/2016/e/e2/T--HUST-China--notebook-patp2-R-CI-TT-fig01.png" alt="">
| |
| </p> | | </p> |
| <p> | | <p> |
Line 2,069: |
Line 2,095: |
| </tr> | | </tr> |
| </table> | | </table> |
− | <img src="https://static.igem.org/mediawiki/2016/d/d1/T--HUST-China--notebook-Whole_circuit_of_app-fig01.png" alt=""> | + | <img src="https://static.igem.org/mediawiki/2016/9/96/T--HUST-China--notebook-R-iLDH-T-p-R-β-fig02.png" alt=""> |
| </p> | | </p> |
| </div> | | </div> |
Line 2,134: |
Line 2,160: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td>Duet-pet | + | <td>pETDuet-1 |
| <td>EcoRI and PstI</td> | | <td>EcoRI and PstI</td> |
| </tr> | | </tr> |
Line 2,140: |
Line 2,166: |
| <td>plac-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase -psb1c3 | | <td>plac-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase -psb1c3 |
| <td>EcoRI and PstI</td> | | <td>EcoRI and PstI</td> |
− | </tr>
| |
− | </table>
| |
− | </p>
| |
− | <p>
| |
− | <h6>9.7</h6>
| |
− | <h6>Transformation</h6>
| |
− | <h6>Zhenyi Guo</h6>
| |
− | <table border="1">
| |
− | <tr>
| |
− | <td>Name
| |
− | <td>Strain
| |
− | <td>Medium</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>plac-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
| |
− | <td>DH5α
| |
− | <td>LB(Chl added)</td>
| |
| </tr> | | </tr> |
| </table> | | </table> |
Line 2,173: |
Line 2,182: |
| <tr> | | <tr> |
| <td>plac-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase | | <td>plac-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase |
− | <td>Duet-pet(T7)(cut by EcoRI and PstI) | + | <td>pETDuet-1(cut by EcoRI and PstI) |
| <td>DH5α | | <td>DH5α |
| <td>LB(Amp added) </td> | | <td>LB(Amp added) </td> |
Line 2,182: |
Line 2,191: |
| <h6>9.18</h6> | | <h6>9.18</h6> |
| <h6>Colony PCR</h6> | | <h6>Colony PCR</h6> |
− | <h6>Successfully align the sequencing results with the objective sequences:</h6>
| |
| <h6>Zhenyi Guo</h6> | | <h6>Zhenyi Guo</h6> |
| <table border="1"> | | <table border="1"> |
Line 2,198: |
Line 2,206: |
| </p> | | </p> |
| </div> | | </div> |
| + | <div> |
| + | <h2 class="title"> PRE-RBS-Cro-RBS-CII-TT-ptrp </h2> |
| + | <p> |
| + | <h6>10.1</h6> |
| + | <h6>PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Total</td> |
| + | </tr> |
| + | <tr> |
| + | <td> PRE-RBS-Cro-RBS-CII-TT-ptrp |
| + | <td>50 μl</td> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.2</h6> |
| + | <h6>Restriction Enzyme Digestion</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Enzyme</td> |
| + | </tr> |
| + | <tr> |
| + | <td> Psb1c3 |
| + | <td> XbalI and PstI </td> |
| + | </tr> |
| + | </table> |
| + | <img src="https://static.igem.org/mediawiki/2016/3/3d/T--HUST-China--GZY-2.jpeg"width="85%" |
| + | alt=""> |
| + | </p> |
| + | |
| + | <h6>10.3</h6> |
| + | <h6>DNA ligation&transformation</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name</td> |
| + | <td>Backbone</td> |
| + | <td>Strain</td> |
| + | <td>Medium</td> |
| + | </tr> |
| + | <tr> |
| + | <td> PRE-RBS-Cro-RBS-CII-TT-ptrp </td> |
| + | <td>psb1c3</td> |
| + | <td>DH5α</td> |
| + | <td>LB(chl added) </td> |
| + | </tr> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | |
| + | |
| + | <h6>10.4</h6> |
| + | <h6>Colony PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Number</td> |
| + | <td>Name</td> |
| + | </tr> |
| + | <tr> |
| + | <td>1</td> |
| + | <td> PRE-RBS-Cro-RBS-CII-TT-ptrp- PSB1C3</td> |
| + | </tr> |
| + | </table> |
| + | <img src="https://static.igem.org/mediawiki/2016/6/65/T--HUST-China--GZY-4.jpeg"width="85%" |
| + | alt=""> |
| + | </p> |
| + | |
| + | </div> |
| + | |
| + | <div> |
| + | <h2 class="title"> RBS-CI-TT-pR-RBS-CIII-RBS-RFPtag-TT-pRM-RBS-GFPtag </h2> |
| + | <p> |
| + | <h6>10.1</h6> |
| + | <h6>PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Total</td> |
| + | </tr> |
| + | <tr> |
| + | <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFPtag-TT-pRM-RBS-GFPtag <td>50 μl</td> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.2</h6> |
| + | <h6>Restriction Enzyme Digestion</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Enzyme</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Psb1c3</td> |
| + | <td>XbalI and PstI</td> |
| + | </tr> |
| + | <tr> |
| + | <td>RBS-CI-TT-pR-RBS-CIII-RBS-RFPtag-TT-pRM-RBS-GFPtag</td> |
| + | <td>XbalI and PstI</td> |
| + | </tr> |
| + | </table> |
| + | <img src=" https://static.igem.org/mediawiki/2016/8/89/T--HUST-China--GZY-3.jpeg"width="85%" |
| + | alt=""> |
| + | </p> |
| + | |
| + | |
| + | <h6>10.3</h6> |
| + | <h6>DNA ligation&transformation</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name</td> |
| + | <td>Backbone</td> |
| + | <td>Strain</td> |
| + | <td>Medium</td> |
| + | </tr> |
| + | <tr> |
| + | <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFPtag-TT-pRM-RBS-GFPtag </td> |
| + | <td>psb1c3</td> |
| + | <td>DH5α</td> |
| + | <td>LB(chl added) </td> |
| + | </tr> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.4</h6> |
| + | <h6>Colony PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Number</td> |
| + | <td>Name</td> |
| + | </tr> |
| + | <tr> |
| + | <td>1</td> |
| + | <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFPtag-TT-pRM-RBS-GFPtag - Ppsb1c3</td> |
| + | </tr> |
| + | </table> |
| + | <img src=" https://static.igem.org/mediawiki/2016/7/7f/T--HUST-China--GZY-5.jpeg"width="85%" |
| + | alt=""> |
| + | </p> |
| + | |
| + | </div> |
| + | |
| + | <div> |
| + | <h2 class="title"> pCyc-Kozak-ABF2-TTADH1-Prd29A- Kozak-GFP-LVAtag </h2> |
| + | <p> |
| + | <h6>10.1</h6> |
| + | <h6>PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Total</td> |
| + | </tr> |
| + | <tr> |
| + | <td> Kozak-ABF2-TTADH1-pRD29A-Kozak-GFP-LVAtag |
| + | <td>50 μl</td> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.2</h6> |
| + | <h6>Restriction Enzyme Digestion</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Enzyme</td> |
| + | </tr> |
| + | <td>pCyc-psb1c3</td> |
| + | <td>EcoRI and XbalI</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Kozak-ABF2-TTADH1-pRD29A-Kozak-GFP-LVAtag</td> |
| + | <td>XbalI and PstI</td> |
| + | </tr> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.3</h6> |
| + | <h6>DNA ligation&transformation</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name</td> |
| + | <td>Backbone</td> |
| + | <td>Strain</td> |
| + | <td>Medium</td> |
| + | </tr> |
| + | <tr> |
| + | <td> Kozak-ABF2-TTADH1-pRD29A-Kozak-GFP-LVAtag </td> |
| + | <td>psb1c3</td> |
| + | <td>DH5α</td> |
| + | <td>LB(chl added) </td> |
| + | </tr> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.4</h6> |
| + | <h6>Colony PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Number</td> |
| + | <td>Name</td> |
| + | </tr> |
| + | <tr> |
| + | <td>1</td> |
| + | <td> pCyc-Kozak-ABF2-TTADH1-pRD29A-Kozak-GFP-LVAtag -psb1c3</td> |
| + | </tr> |
| + | </table> |
| + | </p> |
| + | </div> |
| + | |
| + | <div> |
| + | <h2 class="title"> RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase-Placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII </h2> |
| + | <p> |
| + | <h6>10.6</h6> |
| + | <h6>PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Total</td> |
| + | </tr> |
| + | <tr> |
| + | <td> RBS-ompA-iLDH <td>50 μl</td> |
| + | </tr> |
| + | <tr> |
| + | <td>TT-pRM-RBS-beta-galactosidase</td> |
| + | <td>50μl</td> |
| + | |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.7</h6> |
| + | <h6>Restriction Enzyme Digestion</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name |
| + | <td>Enzyme</td> |
| + | </tr> |
| + | <tr> |
| + | <td> Placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-psb1c3 <td> EcoRI and XbalI </td> |
| + | </tr> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.8</h6> |
| + | <h6>DNA ligation&transformation</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Name</td> |
| + | <td>Backbone</td> |
| + | <td>Strain</td> |
| + | <td>Medium</td> |
| + | </tr> |
| + | <tr> |
| + | <td> RBS-ompA-iLDH、TT-pRM-RBS-beta-galactosidase </td> |
| + | <td> Placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-psb1c3</td> |
| + | <td>DH5α</td> |
| + | <td>LB(chl added) </td> |
| + | </tr> |
| + | </table> |
| + | </p> |
| + | <p> |
| + | <h6>10.9</h6> |
| + | <h6>Colony PCR</h6> |
| + | <h6> Zhenyi Guo </h6> |
| + | <table border="1"> |
| + | <tr> |
| + | <td>Number</td> |
| + | <td>Name</td> |
| + | </tr> |
| + | <tr> |
| + | <td>1</td> |
| + | <td> RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase-Placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-psb1c3</td> |
| + | </tr> |
| + | </table> |
| + | <img src="https://static.igem.org/mediawiki/2016/d/d8/T--HUST-China--GZY-1.jpeg" width="85%" |
| + | alt=""> |
| + | </p> |
| + | |
| + | |
| + | </div> |
| + | |
| </section> | | </section> |
| </div> | | </div> |
| </div> | | </div> |
| + | </div> |
| <div class="returnTop"> | | <div class="returnTop"> |
| <img src="https://static.igem.org/mediawiki/2016/f/f0/T--HUST-China--returnTop.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/f/f0/T--HUST-China--returnTop.png" alt=""> |
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| }); | | }); |
| }); | | }); |
− |
| |
| </script> | | </script> |
| </body> | | </body> |
| | | |
| </html> | | </html> |