Difference between revisions of "Team:Exeter/Log"

 
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You can see below the team photo taken the other day.
 
You can see below the team photo taken the other day.
 
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Today we were given a talk by Markus Gershater, the cofounder of Synthace
 
Today we were given a talk by Markus Gershater, the cofounder of Synthace
 
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We discussed the kill switch idea more in depth, looking at the possibility of using a lab grown amino acid to trigger the kill switch.
 
We discussed the kill switch idea more in depth, looking at the possibility of using a lab grown amino acid to trigger the kill switch.
 
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In addition to this, we started developing an instruction manual to accompany the game.
 
In addition to this, we started developing an instruction manual to accompany the game.
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Some of the team spoke to Dr Mark Ramsdale, a fungal cell biologist. He gave feedback on the PAF dea, raising the question as to why we wanted to express PAF in E. coli as opposed to a fungus. Dr Ramsdale pointed out that due to how small PAF was as a protein, using GFP as a tag could affect how it is transported around the cell, and therefore skew the results. He told us about fungi specific promoters in Neurospora crassa which acts as a Circadian Clock using light and heat as inducers.
 
Some of the team spoke to Dr Mark Ramsdale, a fungal cell biologist. He gave feedback on the PAF dea, raising the question as to why we wanted to express PAF in E. coli as opposed to a fungus. Dr Ramsdale pointed out that due to how small PAF was as a protein, using GFP as a tag could affect how it is transported around the cell, and therefore skew the results. He told us about fungi specific promoters in Neurospora crassa which acts as a Circadian Clock using light and heat as inducers.
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We also met with Professor Rob Beardmore to discuss the genome integration idea. He said it sounds interesting but raised concerns about if it would even be possible to mathematically model it. He also liked the killswitch idea and said testing KillerRed and KillerOrange under a gradient of slowly increasing light intensity could be the strongest selector for resistance.
 
We also met with Professor Rob Beardmore to discuss the genome integration idea. He said it sounds interesting but raised concerns about if it would even be possible to mathematically model it. He also liked the killswitch idea and said testing KillerRed and KillerOrange under a gradient of slowly increasing light intensity could be the strongest selector for resistance.
 
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We also experimented with the vlog format, testing out the dynamics of using three people instead of two which we felt it worked a lot better.
 
We also experimented with the vlog format, testing out the dynamics of using three people instead of two which we felt it worked a lot better.
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Also, we completed two presentations on Synthetic Biology and iGEM which we intend to run on a loop on two iPads for the duration of the fair.
 
Also, we completed two presentations on Synthetic Biology and iGEM which we intend to run on a loop on two iPads for the duration of the fair.
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<p id="pp">Leanne and Emily met with Dr Nicky King again to discuss what we have so far for the synthetic biology module we are writing. It was incredibly useful, as Dr King gave us advice on several different module structures we could look at and several other academics we could talk to. She seems very enthusiastic about our idea, offering to propose it to the Natural Sciences and the Biosciences committee at the start of first term to find out if it’s something we can implement.</p>
 
<p id="pp">Leanne and Emily met with Dr Nicky King again to discuss what we have so far for the synthetic biology module we are writing. It was incredibly useful, as Dr King gave us advice on several different module structures we could look at and several other academics we could talk to. She seems very enthusiastic about our idea, offering to propose it to the Natural Sciences and the Biosciences committee at the start of first term to find out if it’s something we can implement.</p>
 
<p id="pp">We also redid headshots and our team photo, as everyone was present today and the previous image sizes were not appropriate for the wiki. We definitely like these ones, and it’s great to have the whole student side of the team together!</p>
 
<p id="pp">We also redid headshots and our team photo, as everyone was present today and the previous image sizes were not appropriate for the wiki. We definitely like these ones, and it’s great to have the whole student side of the team together!</p>
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<p id="pp">We mailed the Killer Orange spread plate to the Glasgow iGEM team for the collaboration, and they’ll be posting the Z1 lac-repressor E. coli strains to us tomorrow morning. Dr Tom Ellis responded to an email from Jack, giving his definition of a kill switch and bringing up potential ways to build upon the project if more time was available (like constructing an almost 100% efficient operon system).</p>
 
<p id="pp">We mailed the Killer Orange spread plate to the Glasgow iGEM team for the collaboration, and they’ll be posting the Z1 lac-repressor E. coli strains to us tomorrow morning. Dr Tom Ellis responded to an email from Jack, giving his definition of a kill switch and bringing up potential ways to build upon the project if more time was available (like constructing an almost 100% efficient operon system).</p>
 
<p id="pp">Joel designed a title banner for the wiki over the weekend which incorporated our colour scheme, logo and title (pictured just below).</p>
 
<p id="pp">Joel designed a title banner for the wiki over the weekend which incorporated our colour scheme, logo and title (pictured just below).</p>
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<p id="pp">Today’s lab team consisted of Dan, Emily, Pablo and Joel. They prepared cultures of Killer Red and Killer Orange for an upcoming experiment, and cleaned the mini stat chambers so they can be autoclaved and then used again.</p>
 
<p id="pp">Today’s lab team consisted of Dan, Emily, Pablo and Joel. They prepared cultures of Killer Red and Killer Orange for an upcoming experiment, and cleaned the mini stat chambers so they can be autoclaved and then used again.</p>
 
<p id="pp">They transformed DNAse1 and sent samples of the miniprep off for sequencing. They also prepared overnights of Killer Red and Killer Orange for repeat experiments, and performed overnights for the Interlab Study.</p>
 
<p id="pp">They transformed DNAse1 and sent samples of the miniprep off for sequencing. They also prepared overnights of Killer Red and Killer Orange for repeat experiments, and performed overnights for the Interlab Study.</p>

Latest revision as of 01:45, 20 October 2016