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− | <div class="wcontent-box"> | + | |
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | |||
+ | <script type="text/javascript" src="https://2016.igem.org/Template:Waterloo/angular?action=raw&ctype=text/javascript"></script> | ||
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+ | <!-- this last link is actually loading fontawesome even though it's just called CSS --> | ||
+ | <link rel="stylesheet" href="https://2016.igem.org/Team:Waterloo/CSS?action=raw&ctype=text/css" type="text/css" /> | ||
+ | |||
+ | </head> | ||
+ | <div ng-controller="team" ng-app="waterloo"> | ||
+ | |||
+ | <header> | ||
+ | |||
+ | <div class="cd-auto-hide-header" ng-mouseleave="hider()" ng-controller="nav" ng-cloak> | ||
+ | <a href="/Team:Waterloo"><img style="height: 100%; padding-left: 7vw;" src="https://static.igem.org/mediawiki/2016/7/7e/T--Waterloo--WATERLOgo.jpg"> </a> | ||
+ | <nav class="cd-primary-nav"> | ||
+ | <a href="#cd-navigation" class="nav-trigger"> | ||
+ | <span> | ||
+ | <em aria-hidden="true"></em> | ||
+ | Menu | ||
+ | </span> | ||
+ | </a> | ||
+ | <!-- .nav-trigger --> | ||
+ | |||
+ | <ul id="cd-navigation" class="mobile-menu"> | ||
+ | <li onclick="$(this).toggleClass('show-secondary-nav');"><a href="#" ng-click="clickety=true" ng-mouseover="subnav='project'; ">PROJECT</a> | ||
+ | <ul class="secondary-nav"> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Description">DESCRIPTION</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Design">DESIGN</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Requirements">REQUIREMENTS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Model">MODELLING</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li onclick="$(this).toggleClass('show-secondary-nav');"><a href="#" ng-click="clickety=true" ng-mouseover="subnav='design';">BLUEPRINT</a> | ||
+ | <ul class="secondary-nav"> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/HowItWorks">HOW IT WORKS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Software">PREMATURE STOP CODON</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/PromoterSelection">PROMOTER SELECTION</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/PlasmidLossModel">PLASMID LOSS</a></li> | ||
+ | <li> <a class="paragraph-medium" href="/Team:Waterloo/NetworkModelling">NETWORK MODELLING</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Aggregation">AGGREGATION MODEL</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/Crispr">CRISPR</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li onclick="$(this).toggleClass('show-secondary-nav');"><a href="#" ng-click="clickety=true" ng-mouseover="subnav='results';">RESULTS</a> | ||
+ | <ul class="secondary-nav"> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Proof">PROOF OF CONCEPT</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/PlasmidLossModel">PLASMID LOSS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Results/PromoterAnalysis">PROMOTER ANALYSIS</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li onclick="$(this).toggleClass('show-secondary-nav');"><a href="#" ng-click="clickety=true" ng-mouseover="subnav='lab'">LAB</a> | ||
+ | <ul class="secondary-nav"> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Parts">PARTS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Experiments">PROTOCOLS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Notebook">NOTEBOOK</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Safety">SAFETY</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li onclick="$(this).toggleClass('show-secondary-nav');"><a href="#" ng-click="clickety=true" ng-mouseover="subnav='practices'">POLICY</a> | ||
+ | <ul class="secondary-nav"> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/HP/Silver">SILVER</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/HP/Gold">GOLD</a></li> | ||
+ | <li><a class="paragraph-medium" style="font-weight: bold; color: #cd5f94" href="/Team:Waterloo/Engagement">ENGAGEMENT</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #cd5f94" href="/Team:Waterloo/Engagement/GenderEquity">GENDER EQUITY</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #cd5f94" href="/Team:Waterloo/Engagement/Ethics">ETHICS</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #cd5f94" href="/Team:Waterloo/Engagement/Access">ACCESS</a></li> | ||
+ | <li><a class="paragraph-medium" style="font-weight:bold; color: #5f94cd;" href="/Team:Waterloo/Integrated_Practices">INTEGRATED PRACTICES</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #5f94cd;" href="/Team:Waterloo/Integrated_Practices/OpenScience">OPEN SCIENCE</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #5f94cd;" href="/Team:Waterloo/Integrated_Practices/Networks">NETWORKS</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li onclick="$(this).toggleClass('show-secondary-nav');"><a href="#" ng-click="clickety=true" ng-mouseover="subnav='team';">TEAM</a> | ||
+ | <ul class="secondary-nav"> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Team">THE TEAM</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Attributions">ATTRIBUTIONS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Sponsors">SPONSORS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Collaborations">COLLABORATION</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- .cd-primary-nav --> | ||
+ | |||
+ | <nav class="cd-secondary-nav" id="projecttab" ng-show="subnav=='project'"> | ||
+ | <ul> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Description">DESCRIPTION</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Design">DESIGN</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Requirements">REQUIREMENTS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Model">MODELLING</a></li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- .cd-secondary-nav --> | ||
+ | <nav class="cd-secondary-nav" id="designtab" ng-show="subnav == 'design'"> | ||
+ | <ul> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/HowItWorks">HOW IT WORKS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Software">PREMATURE STOP CODON</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/PromoterSelection">PROMOTER SELECTION</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/PlasmidLossModel">PLASMID LOSS MODEL</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Aggregation">AGGREGATION MODEL</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/InDepthDesign/Crispr">CRISPR</a></li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- .cd-secondary-nav --> | ||
+ | <nav class="cd-secondary-nav" id="resultstab" ng-show="subnav == 'results'"> | ||
+ | <ul> | ||
+ | <li><a class="paragraph-medium" href="https://2016.igem.org/Team:Waterloo/Proof">PROOF OF CONCEPT</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Results/PlasmidLoss">PLASMID LOSS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Results/PromoterAnalysis">PROMOTER ANALYSIS</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- .cd-secondary-nav --> | ||
+ | <nav class="cd-secondary-nav" id="labtab" ng-show="subnav == 'lab'"> | ||
+ | <ul> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Parts">PARTS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Experiments">PROTOCOLS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Notebook">NOTEBOOK</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Safety">SAFETY</a></li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- .cd-secondary-nav --> | ||
+ | <nav class="cd-secondary-nav" id="practices" ng-show="subnav == 'practices'"> | ||
+ | <ul> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/HP/Silver">SILVER</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/HP/Gold">GOLD</a></li> | ||
+ | <li><a class="paragraph-medium" style="font-weight: bold; color: #cd5f94" href="/Team:Waterloo/Engagement">ENGAGEMENT</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #cd5f94" href="/Team:Waterloo/Engagement/GenderEquity">GENDER EQUITY</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #cd5f94" href="/Team:Waterloo/Engagement/Ethics">ETHICS</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #cd5f94" href="/Team:Waterloo/Engagement/Access">ACCESS</a></li> | ||
+ | |||
+ | |||
+ | <li><a class="paragraph-medium" style="font-weight:bold; color: #5f94cd;" href="/Team:Waterloo/Integrated_Practices">INTEGRATED PRACTICES</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #5f94cd;" href="/Team:Waterloo/Integrated_Practices/OpenScience">OPEN SCIENCE</a></li> | ||
+ | <li><a class="paragraph-medium" style="color: #5f94cd;" href="/Team:Waterloo/Integrated_Practices/Networks">NETWORKS</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- .cd-secondary-nav --> | ||
+ | <nav class="cd-secondary-nav" id="teamtab" ng-show="subnav == 'team'"> | ||
+ | <ul> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Team">THE TEAM</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Attributions">ATTRIBUTIONS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Sponsors">SPONSORS</a></li> | ||
+ | <li><a class="paragraph-medium" href="/Team:Waterloo/Collaborations">COLLABORATION</a></li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- .cd-secondary-nav --> | ||
+ | |||
+ | </div> | ||
+ | <!-- .cd-auto-hide-header --> | ||
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+ | |||
+ | |||
+ | |||
+ | <div class="container-fluid"> | ||
+ | |||
+ | <div class="idkmakeityellow"> | ||
+ | <div class="header-image" style="background-image: url(https://static.igem.org/mediawiki/2016/1/19/T--Waterloo--awards.jpg);"> | ||
+ | <h3 class="header-text"><span style="background-color: #FFFFFF;opacity: 0.8;">Requirements</span></h3> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div ng-controller="medals"> | ||
+ | |||
+ | <div class="clickmepls"> | ||
+ | |||
+ | <div class="medal"> | ||
+ | <a href="" ng-click="active='bronze'"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/0/0b/T--Waterloo--Bronze_medal.png"> | ||
+ | </a> | ||
+ | </div> | ||
+ | |||
+ | <div class="medal"> | ||
+ | <a href="" ng-click="active='silver'"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/a/a1/T--Waterloo--Silver_medal.png"> | ||
+ | </a> | ||
+ | </div> | ||
+ | |||
+ | <div class="medal"> | ||
+ | <a href="" ng-click="active='gold'"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/c/c7/T--Waterloo--Gold_medal.png"> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="wcontent-box" ng-show="active == 'bronze'"> | ||
<div class="wcontent-title"> | <div class="wcontent-title"> | ||
Bronze Requirements | Bronze Requirements | ||
</div> | </div> | ||
− | <div class="wcontent"> | + | <div class="wcontent" id="bronze"> |
<ol class="requirement-text"> | <ol class="requirement-text"> | ||
<li>Register and Attend | <li>Register and Attend | ||
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</div> | </div> | ||
− | <div class="wcontent-box"> | + | <div class="wcontent-box" ng-show=" active == 'silver'"> |
− | <div class="wcontent-title"> | + | <div class="wcontent-title" id="silver"> |
Silver Requirements | Silver Requirements | ||
</div> | </div> | ||
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<p class="paragraph-medium">Our collaborations with three different Ontario universities in Canada is outlined on our <a href="https://2016.igem.org/Team:Waterloo/Collaboration">Collaboration</a> page.</p></li> | <p class="paragraph-medium">Our collaborations with three different Ontario universities in Canada is outlined on our <a href="https://2016.igem.org/Team:Waterloo/Collaboration">Collaboration</a> page.</p></li> | ||
<li>Human Practices | <li>Human Practices | ||
− | <p class="paragraph-medium">Our Policy and Practices team has delved into multiple areas beyond the lab like the ethics, social justice, and intellectual property rights of our project and the overarching synthetic biology used in it. In addition, our special project looking at the 2015 iGEM collaboration network has revealed some issues. Please see our <a href ="">Practices Overview</a> page for details.</p></li> | + | <p class="paragraph-medium">Our Policy and Practices team has delved into multiple areas beyond the lab like the ethics, social justice, and intellectual property rights of our project and the overarching synthetic biology used in it. In addition, our special project looking at the 2015 iGEM collaboration network has revealed some issues. Please see our <a href ="https://2016.igem.org/Team:Waterloo/Engagement">Practices Overview</a> page for details.</p></li> |
</ol> | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <div class="wcontent-box"> | + | <div class="wcontent-box" ng-show=" active == 'gold' "> |
− | <div class="wcontent-title"> | + | <div class="wcontent-title" id="gold"> |
Gold Requirements | Gold Requirements | ||
</div> | </div> | ||
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<ol class="requirements-text"> | <ol class="requirements-text"> | ||
<li>Integrated Human Practices | <li>Integrated Human Practices | ||
− | <p class="paragraph-medium">We integrated our Human Practices activities into our project where details can be found on the <a href="">Practices Overview</a> page. Moreover, our special investigation on the 2015 iGEM collaborations network issues yielded important results we highly recommend for other iGEM teams where details can also be found on the <a href="">Practices Overview</a> page.</p></li> | + | <p class="paragraph-medium">We integrated our Human Practices activities into our project where details can be found on the <a href="https://2016.igem.org/Team:Waterloo/Engagement">Practices Overview</a> page. Moreover, our special investigation on the 2015 iGEM collaborations network issues yielded important results we highly recommend for other iGEM teams where details can also be found on the <a href="https://2016.igem.org/Team:Waterloo/Engagement">Practices Overview</a> page.</p></li> |
<li>Improve a Previous Part or Project | <li>Improve a Previous Part or Project | ||
− | <p class="paragraph-medium">Building on last year’s BBa_K1645998 BioBrick, we improved its characterization with more robust flow cytometry experiments demonstrating the modified sgRNA’s ability to repress the LacI promoter upstream of an RFP. The related documented information can be found <a href="">here</a>.</p></li> | + | <p class="paragraph-medium">Building on last year’s BBa_K1645998 BioBrick, we improved its characterization with more robust flow cytometry experiments demonstrating the modified sgRNA’s ability to repress the LacI promoter upstream of an RFP. The related documented information can be found <a href="https://2016.igem.org/Team:Waterloo/Parts">here</a>.</p></li> |
<li>Proof of Concept | <li>Proof of Concept | ||
<p class="paragraph-medium">We demonstrate our proof-of-concept, novel regulatory element functions on our <a href="https://2016.igem.org/Team:Waterloo/Results">Results</a> page.</p></li> | <p class="paragraph-medium">We demonstrate our proof-of-concept, novel regulatory element functions on our <a href="https://2016.igem.org/Team:Waterloo/Results">Results</a> page.</p></li> | ||
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Latest revision as of 01:46, 20 October 2016
Requirements
- Register and Attend
We had a really great summer! We are registered and will be attending the Giant Jamboree Oct. 27-31, 2016.
- Deliverables
Follow this link to our completed Judging Form. Our project is described in our Project Description and our parts can be found on the registry through our Parts page. We will present our project at this year’s Giant Jamboree on Saturday Oct. 29, 2016 in Room 311 at 4:00 PM.
- Attributions
The work done by the students on the Waterloo iGEM team and the assistance we received from our advisors, iGEM teams, graduate students, and many other is displayed on our Attributions page.
- Parts/Contribution
We have added our two BioBricks with documentation on the iGEM Parts Registry: BBa_K2137001 and BBa_K2137002. How these parts are used is discusssed on the Parts page.
- Validated Part
We have validated both of our Biobricks (BBa_K2137001 and BBa_K2137002) and documented its characterization in the iGEM Parts Registry found on our Parts page with an explanation of its utility.
- Collaboration
Our collaborations with three different Ontario universities in Canada is outlined on our Collaboration page.
- Human Practices
Our Policy and Practices team has delved into multiple areas beyond the lab like the ethics, social justice, and intellectual property rights of our project and the overarching synthetic biology used in it. In addition, our special project looking at the 2015 iGEM collaboration network has revealed some issues. Please see our Practices Overview page for details.
- Integrated Human Practices
We integrated our Human Practices activities into our project where details can be found on the Practices Overview page. Moreover, our special investigation on the 2015 iGEM collaborations network issues yielded important results we highly recommend for other iGEM teams where details can also be found on the Practices Overview page.
- Improve a Previous Part or Project
Building on last year’s BBa_K1645998 BioBrick, we improved its characterization with more robust flow cytometry experiments demonstrating the modified sgRNA’s ability to repress the LacI promoter upstream of an RFP. The related documented information can be found here.
- Proof of Concept
We demonstrate our proof-of-concept, novel regulatory element functions on our Results page.