Difference between revisions of "Team:Aix-Marseille/Composite Part"

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(→‎BBa_K1941009 : FliC Desulfovibrio vulgaris producer)
 
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This DNA sequence codes a [http://metacyc.org/gene?orgid=META&id=SCO2782 Lysine decarboxylase] (''Streptomyces coelicolor'') which is an enzyme from the lyase family that converts lysine into cadaverine. The enzyme releases the carbonyl group of the lysin amino acid. Cadaverine (or 1,5-diaminopentane) is a primary diamine which renders the medium alkaline. The lysine decarboxylase is an enzyme whose synthesis is promoted by anaerobiosis and an acidic pH.  
 
This DNA sequence codes a [http://metacyc.org/gene?orgid=META&id=SCO2782 Lysine decarboxylase] (''Streptomyces coelicolor'') which is an enzyme from the lyase family that converts lysine into cadaverine. The enzyme releases the carbonyl group of the lysin amino acid. Cadaverine (or 1,5-diaminopentane) is a primary diamine which renders the medium alkaline. The lysine decarboxylase is an enzyme whose synthesis is promoted by anaerobiosis and an acidic pH.  
 
This enzyme is also the first step in the production of desferrioxame B which is a siderophore.
 
This enzyme is also the first step in the production of desferrioxame B which is a siderophore.
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We succeed to produce DesA under an Arabinose indcution.
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[[File:T--Aix-Marseille--result9.png|center|350px]]
  
 
We registered the original sequence of this subpart in the iGEM registry of standard parts ([http://parts.igem.org/Part:BBa_K1951000 BBa_K1951000]). We optimized our sequence for ''E. coli'' and ordered the synthesis by addition of an inducible promoter.
 
We registered the original sequence of this subpart in the iGEM registry of standard parts ([http://parts.igem.org/Part:BBa_K1951000 BBa_K1951000]). We optimized our sequence for ''E. coli'' and ordered the synthesis by addition of an inducible promoter.
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Siderophores are small, high-affinity iron chelating compounds secreted by microorganisms such as bacteria, fungi and grasses. Siderophores are amongst the strongest soluble Fe3+ binding agents known.
 
Siderophores are small, high-affinity iron chelating compounds secreted by microorganisms such as bacteria, fungi and grasses. Siderophores are amongst the strongest soluble Fe3+ binding agents known.
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- <i>E. coli</i>  TG1 strain with <i>des</i> operon ([http://parts.igem.org/Part:BBa_K1951011 BBa_K1951011]) before and after induction. (You can observe the production of the 4 proteins on the figure on the right; left : before induction, right : after induction)
 
- <i>E. coli</i>  TG1 strain with <i>des</i> operon ([http://parts.igem.org/Part:BBa_K1951011 BBa_K1951011]) before and after induction. (You can observe the production of the 4 proteins on the figure on the right; left : before induction, right : after induction)
  
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Results showed the production of the 4 proteins DesA, DesB, DesC and DesD, all involved in the desferrioxamine B biosynthesis pathway.
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Results showed the production of the 4 proteins DesA, DesB, DesC and DesD, all involved in the desferrioxamine B biosynthesis pathway. We can notice that a leak of the promoter pBAD, indeed we note the production of Des proteins without arabinose induction.
  
 
==Biosorption parts ==
 
==Biosorption parts ==
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The subparts were assembled using standard BioBrick Assembly.
 
The subparts were assembled using standard BioBrick Assembly.
  
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===[http://parts.igem.org/Part:BBa_K1941009 BBa_K1941009] : FliC <i> ''Desulfovibrio vulgaris'' </i> producer===
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===[http://parts.igem.org/Part:BBa_K1951009 BBa_K1941009] : FliC <i> ''Desulfovibrio vulgaris'' </i> producer===
  
 
====General====
 
====General====
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* There exists a specific innate immune receptor that recognizes flagellin, Toll-like receptor 5 (TLR5). <ref> Kathrani A. & al, 2012 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030117) </ref>
 
* There exists a specific innate immune receptor that recognizes flagellin, Toll-like receptor 5 (TLR5). <ref> Kathrani A. & al, 2012 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030117) </ref>
  
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===[http://parts.igem.org/Part:BBa_K19410010 BBa_K1941010] : CsgA <i> ''Escherichia coli'' </i> producer===
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===[http://parts.igem.org/Part:BBa_K1941010 BBa_K1941010] : CsgA <i> ''Escherichia coli'' </i> producer===
  
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CsgA is the major structural subunit of the curli fimbriae. Curli are coiled surface structures that assemble preferentially at growth temperatures below 37 degrees Celsius. Curli are the major proteinaceous component of a complex extracellular matrix produced by many ''Enterobacteriaceae''. Curli were first discovered in the late 1980s on ''Escherichia coli'' strains that caused bovine mastitis, and have since been implicated in many physiological and pathogenic processes of ''E. coli'' and ''Salmonella'' spp. Curli fibers are involved in adhesion to surfaces, cell aggregation, and biofilm formation. Curli also mediate host cell adhesion and invasion, and they are potent inducers of the host inflammatory response. The biobrick contains a strong promotor.
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CsgA is the major structural subunit of the curli fimbriae. Curli fibers are involved in adhesion to surfaces, cell aggregation, and biofilm formation<ref name="Curli">[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838481/ Michelle M. Barnhart and Matthew R. Chapman 2010, Annual Review of Microbiology]</ref>. Curli also mediate host cell adhesion and invasion, and they are potent inducers of the host inflammatory response. The biobrick contains a strong promotor.
  
 
<references/>
 
<references/>
  
 
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Latest revision as of 01:56, 20 October 2016