Difference between revisions of "Team:NUS Singapore/Parts"

 
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<span><img src="https://static.igem.org/mediawiki/2016/1/1e/T--NUS_Singapore--practice.png" style="width: 30px; height: auto; top: 30px; left: 35px; position: absolute;"><h4 style=" left: 75px; top: 20px; position: absolute; font-size: 17px;">NUS_SINGAPORE</h4></span>
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<span><img src="https://static.igem.org/mediawiki/2016/1/1e/T--NUS_Singapore--practice.png" style="width: 30px; height: auto; top: 30px; left: 35px; position: absolute;"><h4 style=" left: 75px; top: 20px; position: absolute; font-size: 17px; color: white;">NUS_SINGAPORE</h4></span>
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Project/Wet_Lab" class="list-group-item" style="color: rgb(179, 179, 179);">Overview</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Project/Wet_Lab" class="list-group-item" style="color: rgb(179, 179, 179);">Overview</a>
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<a href="https://2016.igem.org/Team:NUS_Singapore/Model" class="list-group-item" style="color: rgb(179, 179, 179);">Modelling</a>
 
<a href="https://2016.igem.org/Team:NUS_Singapore/Model" class="list-group-item" style="color: rgb(179, 179, 179);">Modelling</a>
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<li><a class="icon icon-notebook"  id="menu-item-9" onclick="growDiv('9')"  href="https://2016.igem.org/Team:NUS_Singapore/Notebook">RIOTSensor
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Description" class="list-group-item" style="color: rgb(179, 179, 179);">Description</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Description" class="list-group-item" style="color: rgb(179, 179, 179);">Description</a>
 
<a href="https://2016.igem.org/Team:NUS_Singapore/Proof" class="list-group-item" style="color: rgb(179, 179, 179);">Proof of Concept</a>
 
<a href="https://2016.igem.org/Team:NUS_Singapore/Proof" class="list-group-item" style="color: rgb(179, 179, 179);">Proof of Concept</a>
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Demonstrate" class="list-group-item" style="color: rgb(179, 179, 179);">Demonstration</a>
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<li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTResponder">RIOTResponder</a></li>
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<li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTInvader">RIOTInvader</a></li>
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<li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTInvader">RIOT Invader</a></li>
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<li><a class="icon icon-practice" onclick="growDiv('3')" id="menu-item-3" href="#">Human Practices
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<span><img id="sidebar-arrow-3" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"><span></a>
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                                                                           <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Silver" class="list-group-item" style="color: rgb(179, 179, 179);">Survey</a>
 
                                                                           <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Silver" class="list-group-item" style="color: rgb(179, 179, 179);">Survey</a>
 
                                                                           <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Gold" class="list-group-item" style="color: rgb(179, 179, 179);">Business Plan</a>
 
                                                                           <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Gold" class="list-group-item" style="color: rgb(179, 179, 179);">Business Plan</a>
 
<a href="https://2016.igem.org/Team:NUS_Singapore/Integrated_Practices" class="list-group-item" style="color: rgb(179, 179, 179);">Integration</a>
 
<a href="https://2016.igem.org/Team:NUS_Singapore/Integrated_Practices" class="list-group-item" style="color: rgb(179, 179, 179);">Integration</a>
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<li><a class="icon icon-collaboration" onclick="growDiv('4')" id="menu-item-4" href="#">Collaborations
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations" class="list-group-item" style="color: rgb(179, 179, 179);">Melbourne</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#MELBOURNE" class="list-group-item" style="color: rgb(179, 179, 179);">MELBOURNE</a>
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations" class="list-group-item" style="color: rgb(179, 179, 179);">Hong_Kong_HKU</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKUST" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKUST</a>
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations" class="list-group-item" style="color: rgb(179, 179, 179);">ETH_Zurich</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKU" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKU</a>
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                                                         <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Parts">Parts Registry</a></li>
 
                                                         <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Parts">Parts Registry</a></li>
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<li><a class="icon icon-safety" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Safety">Safety</a></li>
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                            <li><a class="icon icon-safety" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Safety">Safety</a></li>
  
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<li><a class="icon icon-team" id="menu-item-6" href="https://2016.igem.org/Team:NUS_Singapore/Team">Team</a></li>
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<li><a class="icon icon-notebook"  id="menu-item-7" onclick="growDiv('7')"  href="https://2016.igem.org/Team:NUS_Singapore/Notebook">Notebook
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                            <li><a class="icon icon-notebook"  id="menu-item-7" onclick="growDiv('7')"  href="https://2016.igem.org/Team:NUS_Singapore/Notebook">Notebook
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<span><img id="sidebar-arrow-7" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a>
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                                <span><img id="sidebar-arrow-7" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a>
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group1" class="list-group-item" style="color: rgb(179, 179, 179);">I. RIOT Sensor</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group1" class="list-group-item" style="color: rgb(179, 179, 179);">I. RIOT Sensor</a>
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group2" class="list-group-item" style="color: rgb(179, 179, 179);">II. RIOT Responder</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group2" class="list-group-item" style="color: rgb(179, 179, 179);">II. RIOT Responder</a>
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  <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group3" class="list-group-item" style="color: rgb(179, 179, 179);">III. RIOT Invader</a>
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                                      <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group3" class="list-group-item" style="color: rgb(179, 179, 179);">III. RIOT Invader</a>
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<li><a class="icon icon-acknowledgement" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Attributions">Attributions</a></li>
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                            <li><a class="icon icon-acknowledgement" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Attributions">Attributions</a></li>
 
                             <li><a class="icon icon-cup" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Medals">Medals</a></li>
 
                             <li><a class="icon icon-cup" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Medals">Medals</a></li>
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<header class="major special">
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<h2>Parts Registry</h2>
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                        <h2>Parts Registry</h2>
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                            <header>
 
                             <a name="gold"></a>
 
                             <a name="gold"></a>
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    <p class="medium">RIOT Sensor</p>
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                                <p class="medium">RIOT Sensor</p>
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<p><table>
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                            <p><table>
 
<thead><tr class="tableizer-firstrow"><th>Part Number</th><th>Part Description</th><th>Characterised?</th><th>Link</th></tr></thead><tbody>
 
<thead><tr class="tableizer-firstrow"><th>Part Number</th><th>Part Description</th><th>Characterised?</th><th>Link</th></tr></thead><tbody>
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  <tr><td>BBa_K1897019</td><td>p70-34-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897019</td></tr>
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  <tr><td>BBa_K1897019</td><td>p70-34-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897019">http://parts.igem.org/Part:BBa_K1897019</a></td></tr>
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  <tr><td>BBa_K1897020</td><td>p70-32-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897020</td></tr>
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  <tr><td>BBa_K1897020</td><td>p70-32-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897020">http://parts.igem.org/Part:BBa_K1897020</a></td></tr>
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  <tr><td>BBa_K1897021</td><td>p70-33-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897021</td></tr>
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  <tr><td>BBa_K1897021</td><td>p70-33-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897021">http://parts.igem.org/Part:BBa_K1897021</a></td></tr>
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  <tr><td>BBa_K1897022</td><td>p62-34-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897022</td></tr>
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  <tr><td>BBa_K1897022</td><td>p62-34-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897022">http://parts.igem.org/Part:BBa_K1897022</a></td></tr>
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  <tr><td>BBa_K1897023</td><td>p62-32-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897023</td></tr>
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  <tr><td>BBa_K1897023</td><td>p62-32-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897023">http://parts.igem.org/Part:BBa_K1897023</a></td></tr>
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  <tr><td>BBa_K1897024</td><td>p62-33-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897024</td></tr>
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  <tr><td>BBa_K1897024</td><td>p62-33-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897024">http://parts.igem.org/Part:BBa_K1897024</a></td></tr>
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  <tr><td>BBa_K1897025</td><td>J23100-34-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897025</td></tr>
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  <tr><td>BBa_K1897025</td><td>J23100-34-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897025">http://parts.igem.org/Part:BBa_K1897025</a></td></tr>
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  <tr><td>BBa_K1897026</td><td>J23100-32-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897026</td></tr>
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  <tr><td>BBa_K1897026</td><td>J23100-32-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897026">http://parts.igem.org/Part:BBa_K1897026</a></td></tr>
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  <tr><td>BBa_K1897027</td><td>J23100-33-ALR-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897027</td></tr>
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  <tr><td>BBa_K1897027</td><td>J23100-33-ALR-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897027">http://parts.igem.org/Part:BBa_K1897027</a></td></tr>
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  <tr><td>BBa_K1897028</td><td>p70-33-sfGFP-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897028</td></tr>
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  <tr><td>BBa_K1897028</td><td>p70-33-sfGFP-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897028">http://parts.igem.org/Part:BBa_K1897028</a></td></tr>
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  <tr><td>BBa_K1897029</td><td>p62-33-sfGFP-Terminator </td><td>Yes</td><td>http://parts.igem.org/Part:BBa_K1897029</td></tr>
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  <tr><td>BBa_K1897029</td><td>p62-33-sfGFP-Terminator </td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_K1897029">http://parts.igem.org/Part:BBa_K1897029</a></td></tr>
 
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<p class="medium">RIOT RESPONDER</p>
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<p align=justify>NUS_Singapore is a start-up company formed by a group of undergraduates from the National University of Singapore (NUS), interested in developing new diagnostic tools for cancer using synthetic biology technology. Our start-up uses synthetic biological technology to create the Regulated Invasive Organism Targeting System (RIOT System) which consists of three components: The RIOT Sensor, RIOT Responder and the RIOT Invader. Taken together, this system employs a modular concept of integrating different genetic circuits that serve individual functions with crosstalk capability. For the full and detailed business plan of our start-up, please <a href="https://static.igem.org/mediawiki/2016/7/70/T--NUS_Singapore--BusinessPlanFinal.pdf" style="font-weight: bold;">CLICK HERE.</a></p><br/>
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<thead><tr class="tableizer-firstrow"><th>Part Number</th><th>Part Description</th><th>Characterised?</th><th>Link</th></tr></thead><tbody>
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<tr><td>BBa_K1897000</td><td>HasA coding sequence</td><td>Yes, in BBa_K1897001</td><td><a href="http://parts.igem.org/Part:BBa_ K1897000">http://parts.igem.org/Part:BBa_ K1897000</a></td></tr>
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<tr><td>BBa_K1897001</td><td>HasA expression unit</td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_ K1897001">http://parts.igem.org/Part:BBa_ K1897001</a></td></tr>
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<p class="medium">RIOT Invader</p>
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<tr><td>BBa_K1897003</td><td>HasS coding sequence</td><td>Yes, in BBa_K1897007</td><td><a href="http://parts.igem.org/Part:BBa_ K1897003">http://parts.igem.org/Part:BBa_ K1897003</a></td></tr>
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<tr><td>BBa_K1897005</td><td>HasI coding sequence </td><td>Yes, in BBa_K1897007</td><td><a href="http://parts.igem.org/Part:BBa_ K1897005">http://parts.igem.org/Part:BBa_ K1897005</a></td></tr>
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<p>In cancer treatment, specificity, sensitivity and reliability of diagnosis is the gold criteria of any diagnostic method. The RIOT System employs a novel method of cancer detection, relying on spatial markers that are intrinsic to the tumour microenvironment. Two types of spatial markers are used to increase specificity and ensure that only cancerous cells are targeted. The first spatial marker would be the elevated concentration of lactate usually found around tumours due to the Warburg effect. The second marker would be the detection of CD44v6, a cell surface marker that is upregulated on a variety of cancers. These spatial markers were specially chosen to circumvent a common problem in cancer research, where heterogeneity in different types of cancers results in the lack of a specific diagnostic for a variety of cancers. As elevated lactate concentration is ubiquitous among solid tumours and CD44v6 is present in different types of cancers, our RIOT System has the potential to be a diagnostic that can be applied to multiple cancers, which is advantageous in the current market where broad spectrum diagnostics for cancer are currently lacking. Furthermore, the utility of RIOT System can extend beyond just being a diagnostic and can be modified to deliver a drug payload, potentially acting as both a diagnostic and therapeutic. </p></br>
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<tr><td>BBa_K1897007</td><td>Complete Has operon (controlling expression of LuxR)</td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_ K1897007">http://parts.igem.org/Part:BBa_ K1897007</a></td></tr>
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                            <p><table class="tableizer-table">
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<thead><tr class="tableizer-firstrow"><th>Part Number</th><th>Part Description</th><th>Characterised?</th><th>Link</th></tr></thead><tbody>
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<tr><td>BBa_K1897008</td><td>Constitutively expressed LuxR</td><td>Yes, in BBa_K1897016</td><td><a href="http://parts.igem.org/Part:BBa_ K1897008">http://parts.igem.org/Part:BBa_ K1897008</a></td></tr>
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<tr><td>BBa_K1897009</td><td>pLuxR-controlled LuxI</td><td>Yes, by Western blot of BBa_K1897015</td><td><a href="http://parts.igem.org/Part:BBa_ K1897009">http://parts.igem.org/Part:BBa_ K1897009</a></td></tr>
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<tr><td>BBa_K1897011</td><td>pLuxR-controlled Invasin</td><td>No</td><td></td></tr>
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<tr><td>BBa_K1897013</td><td>pLuxR-controlled Listeriolysin O</td><td>Yes, by Western blot via cotransformation with BBa_K1897008 in pSB4A5</a></td><td><a href="http://parts.igem.org/Part:BBa_ K1897013">http://parts.igem.org/Part:BBa_ K1897013</a></td></tr>
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<tr><td>BBa_K1897014</td><td>pLuxR-controlled GFP</td><td>Yes, in BBa_K1897016</td><td><a href="http://parts.igem.org/Part:BBa_ K1897014">http://parts.igem.org/Part:BBa_ K1897014</a></td></tr>
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<tr><td>BBa_K1897015</td><td>Constitutively expressed LuxR and pLuxR-controlled LuxI</td><td>Yes, by Western blot in pSB1K3</td><td><a href="http://parts.igem.org/Part:BBa_ K1897015">http://parts.igem.org/Part:BBa_ K1897015</a></td></tr>
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<tr><td>BBa_K1897016</td><td>Constitutively expressed LuxR and pLuxR-controlled GFP</td><td>Yes</td><td><a href="http://parts.igem.org/Part:BBa_ K1897016">http://parts.igem.org/Part:BBa_ K1897016</a></td></tr>
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<tr><td>BBa_K1897017</td><td>pLuxR-controlled Invasin and pLuxR-controlled GFP</td><td>No</td><td></td></tr>
  
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<p>Based on our preliminary market analysis of the diagnostics industry, our proposed RIOT System stands out as one of the few in vivo options available for cancer detection. Current cancer diagnostics rely mainly on in vitro laboratory tests for detection of cancer biomarkers (e.g. blood, urine), imaging scans (e.g. CT scans, MRI) or physical examinations to detect the tumour. Thus, our RIOT System offers a radically different diagnostic option that sets itself apart from the current diagnostics. </p></br>
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Latest revision as of 02:03, 20 October 2016


Interactive Points | Codrops

iGEM 2016         NUS SINGAPORE

Parts Registry

RIOT Sensor

Part NumberPart DescriptionCharacterised?Link
BBa_K1897019p70-34-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897019
BBa_K1897020p70-32-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897020
BBa_K1897021p70-33-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897021
BBa_K1897022p62-34-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897022
BBa_K1897023p62-32-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897023
BBa_K1897024p62-33-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897024
BBa_K1897025J23100-34-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897025
BBa_K1897026J23100-32-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897026
BBa_K1897027J23100-33-ALR-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897027
BBa_K1897028p70-33-sfGFP-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897028
BBa_K1897029p62-33-sfGFP-Terminator Yeshttp://parts.igem.org/Part:BBa_K1897029

RIOT RESPONDER

Part NumberPart DescriptionCharacterised?Link
BBa_K1897000HasA coding sequenceYes, in BBa_K1897001http://parts.igem.org/Part:BBa_ K1897000
BBa_K1897001HasA expression unitYeshttp://parts.igem.org/Part:BBa_ K1897001
BBa_K1897003HasS coding sequenceYes, in BBa_K1897007http://parts.igem.org/Part:BBa_ K1897003
BBa_K1897005HasI coding sequence Yes, in BBa_K1897007http://parts.igem.org/Part:BBa_ K1897005
BBa_K1897007Complete Has operon (controlling expression of LuxR)Yeshttp://parts.igem.org/Part:BBa_ K1897007


RIOT Invader

Part NumberPart DescriptionCharacterised?Link
BBa_K1897008Constitutively expressed LuxRYes, in BBa_K1897016http://parts.igem.org/Part:BBa_ K1897008
BBa_K1897009pLuxR-controlled LuxIYes, by Western blot of BBa_K1897015http://parts.igem.org/Part:BBa_ K1897009
BBa_K1897011pLuxR-controlled InvasinNo
BBa_K1897013pLuxR-controlled Listeriolysin OYes, by Western blot via cotransformation with BBa_K1897008 in pSB4A5http://parts.igem.org/Part:BBa_ K1897013
BBa_K1897014pLuxR-controlled GFPYes, in BBa_K1897016http://parts.igem.org/Part:BBa_ K1897014
BBa_K1897015Constitutively expressed LuxR and pLuxR-controlled LuxIYes, by Western blot in pSB1K3http://parts.igem.org/Part:BBa_ K1897015
BBa_K1897016Constitutively expressed LuxR and pLuxR-controlled GFPYeshttp://parts.igem.org/Part:BBa_ K1897016
BBa_K1897017pLuxR-controlled Invasin and pLuxR-controlled GFPNo


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