Difference between revisions of "Team:UNebraska-Lincoln"

 
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/background">Background</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/modeling">Modeling</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments">Experiments</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
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                                                        <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a>
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<span class="sdt_link">Thanks!</span>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions">Attributions</a>
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<h2 style=" height: 90px; font-size:45px, padding-bottom: 30px">Knockout Nitrate</h2>
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<p><font color="Gainsboro">A synthetic biology project by the <a href="http://igem.unl.edu">University of Nebraska-Lincoln iGEM Team</a></font></p>
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</div>
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</section>
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<!-- One -->
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<section id="one" class="wrapper spotlight style1">
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<div class="inner" >
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Description" class="image"><img src="https://static.igem.org/mediawiki/2016/9/90/T--UNebraska-Lincoln--iGEMAstock2.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">Overview</h2>
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<p align="center"><font color="Gainsboro">The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.
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<p>As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!
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</font></p></p>
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</div>
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</div>
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</section>
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<div class="inner" style=''width:70%;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board" class="image"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--UNebraska-Lincoln--iGEMAstock4.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">The Drawing Board</h2>
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<p><font color=Gainsboro>The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">Check out the drawing board!</a></h2>
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</div>
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</section>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory" class="image"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--UNebraska-Lincoln--iGEMAstock3.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">The Laboratory</h2>
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<p><font color=Gainsboro>Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">Check out the Lab Bench!</a></h2>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench" class="image"><img src="https://static.igem.org/mediawiki/2016/e/e9/T--UNebraska-Lincoln--iGEMAstock5.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">Beyond the Bench</h2>
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<p><font color=Gainsboro>Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">Check out our Meeting Room!</a></h2>
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</div>
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<h2 class="major" style="font-size:50px">Important:</h2>
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<section class="features">
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="image"><img src="https://static.igem.org/mediawiki/2016/b/bd/T--UNebraska-Lincoln--sponsors.png" alt="" /></a>
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<h3 class="major">Our Sponsors</h3>
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<p><font color="silver">Our project and iGEM experience would not have been possible without our sponsors!</font></p>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="special">Learn more</a>
 +
</article>
 +
<article>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="image"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--UNebraska-Lincoln--thanks3.png" alt="" /></a>
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<h3 class="major">Attributions</h3>
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<p><font color="silver">Attribution of all external assistance, art, and web design.</font></p>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="special">Learn more</a>
 +
</article>
 +
<article>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="image"><img src="https://static.igem.org/mediawiki/2016/4/4b/T--UNebraska-Lincoln--iGEMAstock6.jpg" alt="" /></a>
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<h3 class="major">Meet the Team!</h3>
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<p><font color="silver">Our highly diverse of team of six undergraduates spans six majors and two countries.</font></p>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="special">Learn more</a>
 +
</article>
 +
<article>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="image"><img src="https://static.igem.org/mediawiki/2016/a/a6/T--UNebraska-Lincoln--SafetyStock.jpg" alt="" /></a>
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<h3 class="major">Safety</h3>
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<p><font color="silver">See the steps we took to ensure our own safety and the safety of our project.</font></p>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="special">Learn more</a>
 +
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<body class="home page page-id-701 page-template page-template-template-home page-template-template-home-php has-sidebar-right">
 
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<div class="container-fluid">
 
−
  <div id="page" class="site">
 
−
  <a class="skip-link screen-reader-text" href="#content">Skip to content</a>
 
−
   
 
−
  <header id="masthead" class="site-header" role="banner">
 
−
    <div class="nav-container">
 
−
      <nav id="site-navigation" class="main-navigation" role="navigation">
 
−
        <div class="container nav-bar">
 
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          <div class="row">
 
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−
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−
              <div class="module widget-handle search-widget-handle left">
 
−
                <div class="search">
 
−
                  <i class="fa fa-search"></i>
 
−
                  <span class="title">Site Search</span>
 
−
                  </div>
 
−
                <div class="function"><form role="search" method="get" id="searchform" class="search-form" action="https://colorlib.com/shapely/" >
 
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−
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−
                   
 
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−
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−
        </nav><!-- #site-navigation -->
 
−
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−
    </header><!-- #masthead -->
 
−
   
 
−
  <div id="content" class="main-container">
 
−
    <section class="content-area ">
 
−
      <div id="main" class="" role="main">
 
−
        <div class="row">
 
−
           
 
−
          <div class="col-md-12"><div id="flexible_home_parallax-3" class="flexible_home_parallax">        <section class="cover fullscreen image-bg" >            <div class="parallax-window fullscreen" data-parallax="scroll" data-image-src="Images/SupplementalLogo.png" >
 
−
              </div>          </section>
 
−
             
 
−
             
 
−
              </div><div id="flexible_home_parallax-7" class="flexible_home_parallax">        <section class="" >                <div class="container">
 
−
                <div class="row align-children">
 
−
                 
 
−
                  <div class="col-md-7 col-sm-6 text-center mb-xs-24">
 
−
                    <img class="cast-shadow" alt="SEO Friendly" src="https://colorlib.com/flexible/wp-content/uploads/sites/12/2016/03/macbook-preview-flexible.png">
 
−
                    </div>
 
−
                  <div class="col-md-4 col-md-offset-1 col-sm-5 col-sm-offset-1">
 
−
                    <div class=""><h3>SEO Friendly</h3><p class="mb32">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam pulvinar luctus sem, eget porta orci. Maecenas molestie dui id diam feugiat, eu tincidunt mauris aliquam. Duis commodo vitae ligula et interdum. Maecenas faucibus mattis imperdiet. In rhoncus ac ligula id ultricies. </p><a class="btn btn-lg btn-filled" href="#">Read More</a>                          </div>
 
−
                    </div>
 
−
                  <!--end of row-->
 
−
                  </div>
 
−
                </div>
 
−
                </section>
 
−
               
 
−
               
 
−
                </div><div id="flexible_home_parallax-8" class="flexible_home_parallax">        <section class="bg-secondary" >                <div class="container">
 
−
                  <div class="row align-children">
 
−
                   
 
−
                   
 
−
                    <div class="col-md-4 col-sm-5 mb-xs-24">
 
−
                      <div class=""><h3>Portfolio Section</h3><p class="mb32">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam pulvinar luctus sem, eget porta orci. Maecenas molestie dui id diam feugiat, eu tincidunt mauris aliquam. Duis commodo vitae ligula et interdum. </p><a class="btn btn-lg btn-filled" href="https://colorlib.com/flexible/portfolio">See It In Action</a>                          </div>
 
−
                    </div>
 
−
                    <!--end of row-->
 
−
                    <div class="col-md-7 col-md-offset-1 col-sm-6 col-sm-offset-1 text-center">
 
−
                      <img class="cast-shadow" alt="Portfolio Section" src="https://colorlib.com/flexible/wp-content/uploads/sites/12/2016/03/flexible-portfolio.png">
 
−
                  </div>                </div>
 
−
                  </div>
 
−
                  </section>
 
−
                 
 
−
                 
 
−
                  </div><div id="flexible_home_parallax-5" class="flexible_home_parallax">        <section class="small-screen image-bg p0" >            <div class="parallax-window " data-parallax="scroll" data-image-src="https://colorlib.com/flexible/wp-content/uploads/sites/12/2016/03/photo-1452723312111-3a7d0db0e024.jpg">
 
−
                    <div class="">
 
−
                      <div class="row">
 
−
                       
 
−
                       
 
−
                        <div class="top-parallax-section">
 
−
                          <div class="col-md-8 col-md-offset-2 col-sm-10 col-sm-offset-1 text-center"><h1>Small Parallax Section</h1><p class="mb32">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus imperdiet rhoncus porta. Ut quis sem quis purus lobortis dictum. Aliquam nec dignissim nisl. Vivamus cursus feugiat sapien, eget tincidunt leo ornare quis.</p><a class="btn btn-lg btn-filled" href="#">More Info</a>                          </div>
 
−
                        </div>
 
−
                        <!--end of row-->
 
−
                      </div>
 
−
                    </div>
 
−
                    </div>          </section>
 
−
                   
 
−
                   
 
−
                    </div><div id="flexible_home_parallax-9" class="flexible_home_parallax">        <section class="bg-secondary pb0" >                <div class="container">
 
−
                      <div class="row">
 
−
                       
 
−
                       
 
−
                        <div class="col-sm-10 col-sm-offset-1 text-center">
 
−
                          <div class="">
 
−
                            <h3>Let's Meet Our Team Member !!!!</h3>
 
−
                          </div>
 
−
                        </div>
 
−
                        <!--end of row-->
 
−
                        <div id="wrapper">
 
−
                          <ul id="index_cards">
 
−
                            <li id="card-1">
 
−
                              <h3>Madison Bierman</h3>
 
−
                              <img src="Images/madison.jpg" height="1200" width="1200" alt="Toucan" />
 
−
                              <p>Name: Toucan<br />
 
−
                                Age: 5<br />
 
−
                                Size: 50 cm<br />
 
−
                                Weight: 600 g<br />
 
−
                                Food: Fruit<br />
 
−
                              Toucan lives in southern and central America and loves to fly around</p>
 
−
                            </li>
 
−
                           
 
−
                            <li id="card-2">
 
−
                              <h3>Colton Harper</h3>
 
−
                              <img src="Images/colton.jpg" height="1200" width="1200" alt="Fox" />
 
−
                              <p>Name: Fox<br />
 
−
                                Age: 3<br />
 
−
                                Size: 70 cm<br />
 
−
                                Weight: 5.5 kg<br />
 
−
                                Food: Meat<br />
 
−
                              Fox lives in the northern hemisphere and loves to play seek and hide</p>
 
−
                            </li>
 
−
                            <li id="card-3">
 
−
                              <h3>Joshua Mueller</h3>
 
−
                              <img src="Images/josh.jpg" height="1080" width="1080" alt="Dog" />
 
−
                              <p>Name: Dog<br />
 
−
                                Age: 8<br />
 
−
                                Size: 120 cm<br />
 
−
                                Weight: 10 kg<br />
 
−
                                Food: Bones<br />
 
−
                              Dog lives in a dog hut and loves to chew shoes, bark and go for walks</p>
 
−
                            </li>
 
−
                            <li id="card-4">
 
−
                              <h3>Phuong Ninh</h3>
 
−
                              <img src="Images/phuong.jpg" height="960" width="960" alt="Penguin" />
 
−
                              <p>Name: Penguin<br />
 
−
                                Age: 20<br />
 
−
                                Size: 110 cm<br />
 
−
                                Weight: 35 kg<br />
 
−
                                Food: Fish<br />
 
−
                              Penguin lives where it's cold and icy and loves to swim and dive</p>
 
−
                            </li>
 
−
                            <li id="card-5">
 
−
                              <h3>Brynne Schwabauer</h3>
 
−
                              <img src="Images/brynne.jpg" height="960" width="960" alt="Lion" />
 
−
                              <p>Name: Lion<br />
 
−
                                Age: 12<br />
 
−
                                Size: 190 cm<br />
 
−
                                Weight: 180 kg<br />
 
−
                                Food: Meat<br />
 
−
                              Lions lives in Africa and loves to sleep most of the day and hunt sometimes</p>
 
−
                            </li>
 
−
                            <li id="card-6">
 
−
                              <h3>Danny Dooling</h3>
 
−
                              <img src="Images/danny.jpg" height="751" width="781" alt="Toucan" />
 
−
                              <p>Name: Toucan<br />
 
−
                                Age: 5<br />
 
−
                                Size: 50 cm<br />
 
−
                                Weight: 600 g<br />
 
−
                                Food: Fruit<br />
 
−
                              Toucan lives in southern and central America and loves to fly around</p>
 
−
                            </li>
 
−
                           
 
−
                          </ul>
 
−
                      </div>                </div>
 
−
                      </div>
 
−
                      </section>
 
−
                     
 
−
                     
 
−
                      </div><div id="flexible_home_portfolio-3" class="flexible_home_portfolio">        <section class="projects bg-dark pb0">
 
−
                        <div class="container">
 
−
                          <div class="col-sm-12 text-center">
 
−
                            <h3 class="mb32">Our Latest Projects</h3>
 
−
                            <p class="mb40">Here is our latest projects. You&#039;ll love them! </p>
 
−
                          </div>
 
−
                        </div>
 
−
                        <div class="row masonry-loader fixed-center fadeOut">
 
−
                          <div class="col-sm-12 text-center">
 
−
                            <div class="spinner"></div>
 
−
                          </div>
 
−
                        </div>
 
−
                        <div class="row masonry masonryFlyIn fadeIn">                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                          <div class="image-tile inner-title hover-reveal text-center">
 
−
                            <a href="Images/1.jpg" title="Project #15">
 
−
                            <img width="2448" height="3264" src="Images/1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1443890484047-5eaa67d1d630" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1443890484047-5eaa67d1d630-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1443890484047-5eaa67d1d630-1-300x209.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1443890484047-5eaa67d1d630-1-768x536.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1443890484047-5eaa67d1d630-1-1024x714.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #15</h5>                            </div>
 
−
                            </a>
 
−
                          </div>
 
−
                          </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                            <div class="image-tile inner-title hover-reveal text-center">
 
−
                              <a href="https://colorlib.com/shapely/portfolio/project-14/" title="Project #14">
 
−
                              <img width="750" height="1334" src="Images/2.jpg" class="attachment-full size-full wp-post-image" alt="photo-1422568374078-27d3842ba676" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1422568374078-27d3842ba676-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1422568374078-27d3842ba676-1-300x209.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1422568374078-27d3842ba676-1-768x536.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1422568374078-27d3842ba676-1-1024x714.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #14</h5>                            </div>
 
−
                              </a>
 
−
                            </div>
 
−
                            </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                              <div class="image-tile inner-title hover-reveal text-center">
 
−
                                <a href="https://colorlib.com/shapely/portfolio/project-13/" title="Project #13">
 
−
                                  <img width="1200" height="853" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447834353189-91c48abf20e1-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1447834353189-91c48abf20e1" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447834353189-91c48abf20e1-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447834353189-91c48abf20e1-1-300x213.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447834353189-91c48abf20e1-1-768x546.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447834353189-91c48abf20e1-1-1024x728.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #13</h5>                            </div>
 
−
                                </a>
 
−
                                </div>
 
−
                              </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                                <div class="image-tile inner-title hover-reveal text-center">
 
−
                                  <a href="https://colorlib.com/shapely/portfolio/project-12-2/" title="Project #12">
 
−
                                    <img width="1200" height="853" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447877085163-3cce903855cd-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1447877085163-3cce903855cd" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447877085163-3cce903855cd-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447877085163-3cce903855cd-1-300x213.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447877085163-3cce903855cd-1-768x546.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447877085163-3cce903855cd-1-1024x728.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #12</h5>                            </div>
 
−
                                  </a>
 
−
                                  </div>
 
−
                                </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                                  <div class="image-tile inner-title hover-reveal text-center">
 
−
                                    <a href="https://colorlib.com/shapely/portfolio/project-12/" title="Project #12">
 
−
                                      <img width="1200" height="853" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447958374760-1ce70cf11ee3-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1447958374760-1ce70cf11ee3" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447958374760-1ce70cf11ee3-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447958374760-1ce70cf11ee3-1-300x213.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447958374760-1ce70cf11ee3-1-768x546.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1447958374760-1ce70cf11ee3-1-1024x728.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #12</h5>                            </div>
 
−
                                    </a>
 
−
                                    </div>
 
−
                                  </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                                    <div class="image-tile inner-title hover-reveal text-center">
 
−
                                      <a href="https://colorlib.com/shapely/portfolio/project-11/" title="Project #11">
 
−
                                        <img width="1200" height="837" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1430329429612-babb42f88673-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1430329429612-babb42f88673" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1430329429612-babb42f88673-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1430329429612-babb42f88673-1-300x209.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1430329429612-babb42f88673-1-768x536.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1430329429612-babb42f88673-1-1024x714.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #11</h5>                            </div>
 
−
                                      </a>
 
−
                                      </div>
 
−
                                    </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                                      <div class="image-tile inner-title hover-reveal text-center">
 
−
                                        <a href="https://colorlib.com/shapely/portfolio/project-10/" title="Project #10">
 
−
                                          <img width="1200" height="853" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449057528837-7ca097b3520c-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1449057528837-7ca097b3520c" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449057528837-7ca097b3520c-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449057528837-7ca097b3520c-1-300x213.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449057528837-7ca097b3520c-1-768x546.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449057528837-7ca097b3520c-1-1024x728.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #10</h5>                            </div>
 
−
                                        </a>
 
−
                                        </div>
 
−
                                      </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                                        <div class="image-tile inner-title hover-reveal text-center">
 
−
                                          <a href="https://colorlib.com/shapely/portfolio/project-9/" title="Project #9">
 
−
                                            <img width="1200" height="837" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449168013943-3a15804bb41c-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1449168013943-3a15804bb41c" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449168013943-3a15804bb41c-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449168013943-3a15804bb41c-1-300x209.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449168013943-3a15804bb41c-1-768x536.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1449168013943-3a15804bb41c-1-1024x714.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #9</h5>                            </div>
 
−
                                          </a>
 
−
                                          </div>
 
−
                                        </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                                          <div class="image-tile inner-title hover-reveal text-center">
 
−
                                            <a href="https://colorlib.com/shapely/portfolio/project-8/" title="Project #8">
 
−
                                              <img width="1200" height="837" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1448518184296-a22facb4446f-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1448518184296-a22facb4446f" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1448518184296-a22facb4446f-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1448518184296-a22facb4446f-1-300x209.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1448518184296-a22facb4446f-1-768x536.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1448518184296-a22facb4446f-1-1024x714.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #8</h5>                            </div>
 
−
                                            </a>
 
−
                                            </div>
 
−
                                          </div>                    <div class="col-md-3 col-sm-6 masonry-item project fadeIn">
 
−
                                            <div class="image-tile inner-title hover-reveal text-center">
 
−
                                              <a href="https://colorlib.com/shapely/portfolio/project-7/" title="Project #7">
 
−
                                                <img width="1200" height="837" src="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1451186859696-371d9477be93-1.jpg" class="attachment-full size-full wp-post-image" alt="photo-1451186859696-371d9477be93" srcset="https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1451186859696-371d9477be93-1.jpg 1200w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1451186859696-371d9477be93-1-300x209.jpg 300w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1451186859696-371d9477be93-1-768x536.jpg 768w, https://colorlib.com/shapely/wp-content/uploads/sites/12/2016/03/photo-1451186859696-371d9477be93-1-1024x714.jpg 1024w" sizes="(max-width: 1200px) 100vw, 1200px" />                            <div class="title"><h5 class="mb0">Project #7</h5>                            </div>
 
−
                                              </a>
 
−
                                              </div>
 
−
                              </div>                </div>      </section>
 
−
                       
 
−
                       
 
−
                        </div><div id="flexible_home_testimonial-3" class="flexible_home_testimonial">
 
−
                          <section class="parallax-section testimonial-section">
 
−
                            <div class="parallax-window" data-parallax="scroll" data-image-src="https://colorlib.com/flexible/wp-content/uploads/sites/12/2016/03/photo-1451417379553-15d8e8f49cde.jpg" style="height: 500px;">
 
−
                              <div class="container align-transform">
 
−
                                <div class="parallax-text image-bg testimonial">
 
−
                                  <div class="row">
 
−
                                    <div class="col-sm-12 text-center">
 
−
                                      <h3>Let meet our Team member</h3>
 
−
                                      </div>
 
−
                                    </div>
 
−
                                  <!--end of row-->
 
−
                                  <div class="row">
 
−
                                    <div class="col-md-8 col-md-offset-2 col-sm-10 col-sm-offset-1">
 
−
                                      <div class="text-slider slider-arrow-controls text-center relative">
 
−
                                        <ul class="slides" style="overflow: hidden;">                                                                                    <li>
 
−
                                          <p><p>Integer ut rutrum nulla, sit amet fringilla nisi. Nam nisl velit, vehicula at nibh sed, porttitor sollicitudin ante. Sed justo augue, vestibulum ut efficitur vitae, euismod et justo. Mauris sed mattis ante. Ut ligula lectus, consequat vitae neque ac, gravida hendrerit ante.</p>
 
−
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Latest revision as of 02:44, 20 October 2016

Solid State by HTML5 UP

Overview

The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.

As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!

The Drawing Board

The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.

Check out the drawing board!

The Laboratory

Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.

Check out the Lab Bench!

Beyond the Bench

Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!

Check out our Meeting Room!

Important:

Our Sponsors

Our project and iGEM experience would not have been possible without our sponsors!

Learn more

Attributions

Attribution of all external assistance, art, and web design.

Learn more

Meet the Team!

Our highly diverse of team of six undergraduates spans six majors and two countries.

Learn more

Safety

See the steps we took to ensure our own safety and the safety of our project.

Learn more