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| + | <ul id="sdt_menu" class="sdt_menu"> | ||
| + | <li style="background-color:#4c5c96;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/0/02/T--UNebraska-Lincoln--home2.png" alt=""/> | ||
| + | <span class="sdt_active"></span> | ||
| + | <span class="sdt_wrap"> | ||
| + | <span class="sdt_link">Home</span> | ||
| + | <span class="sdt_descr"></span> | ||
| + | </span> | ||
| + | </a> | ||
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| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/c/cb/T--UNebraska-Lincoln--TeamStock2.png" alt=""/> | ||
| + | <span class="sdt_active"></span> | ||
| + | <span class="sdt_wrap"> | ||
| + | <span class="sdt_link" >Team</span> | ||
| + | <span class="sdt_descr"></span> | ||
| + | </span> | ||
| + | </a> | ||
| + | </li> | ||
| + | <li style="background-color:#4c5c96;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/1/11/T--UNebraska-Lincoln--Blueprints2.png" alt=""/> | ||
| + | <span class="sdt_active"></span> | ||
| + | <span class="sdt_wrap"> | ||
| + | <span class="sdt_link">Blueprints</span> | ||
| + | <span class="sdt_descr"></span> | ||
| + | </span> | ||
| + | </a> | ||
| + | <div class="sdt_box"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/background">Background</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/modeling">Modeling</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/design">Design</a> | ||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | </li> | ||
| + | <li style="background-color:#4c5c96;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/a/a3/T--UNebraska-Lincoln--Laboratory2.png" alt=""/> | ||
| + | <span class="sdt_active"></span> | ||
| + | <span class="sdt_wrap"> | ||
| + | <span class="sdt_link" style="">Laboratory</span> | ||
| + | <span class="sdt_descr"></span> | ||
| + | <div class="sdt_box"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments">Experiments</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/lab_manual">Lab Notebook</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/safety_cases">Safety Cases</a> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </span> | ||
| + | </a> | ||
| + | </li> | ||
| + | <li style="background-color:#4c5c96;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/1/1a/T--UNebraska-Lincoln--beyondthebench2.png" alt=""/> | ||
| + | <span class="sdt_active"></span> | ||
| + | <span class="sdt_wrap"> | ||
| + | <span class="sdt_link">Outreach</span> | ||
| + | <span class="sdt_descr"></span> | ||
| + | <div class="sdt_box"> | ||
| + | |||
| + | |||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </span> | ||
| + | </a> | ||
| + | </li> | ||
| + | <li style="background-color:#4c5c96;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/> | ||
| + | <span class="sdt_active"></span> | ||
| + | <span class="sdt_wrap"> | ||
| + | <span class="sdt_link">Thanks!</span> | ||
| + | <span class="sdt_descr"></span> | ||
| + | <div class="sdt_box"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions">Attributions</a> | ||
| + | |||
| + | </div> | ||
| + | </span> | ||
| + | </a> | ||
| + | </li> | ||
| + | |||
| + | </ul> | ||
| + | |||
| + | </div> | ||
</div> | </div> | ||
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| − | |||
| − | + | <!-- Banner --> | |
| − | </p> | + | <section id="banner" > |
| + | <div class="inner" > | ||
| + | <h2 style=" height: 90px; font-size:45px, padding-bottom: 30px">Knockout Nitrate</h2> | ||
| + | <p><font color="Gainsboro">A synthetic biology project by the <a href="http://igem.unl.edu">University of Nebraska-Lincoln iGEM Team</a></font></p> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | <!-- One --> | ||
| + | <section id="one" class="wrapper spotlight style1"> | ||
| + | <div class="inner" > | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Description" class="image"><img src="https://static.igem.org/mediawiki/2016/9/90/T--UNebraska-Lincoln--iGEMAstock2.jpg" alt="" /></a> | ||
| + | <div class="content"> | ||
| + | <h2 class="major" style="font-size:50px">Overview</h2> | ||
| + | <p align="center"><font color="Gainsboro">The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system. | ||
| + | <p>As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy! | ||
| + | </font></p></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | <!-- Two --> | ||
| + | <section id="three" class="wrapper alt spotlight style2"> | ||
| + | <div class="inner" style=''width:70%;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board" class="image"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--UNebraska-Lincoln--iGEMAstock4.jpg" alt="" /></a> | ||
| + | <div class="content"> | ||
| + | <h2 class="major" style="font-size:50px">The Drawing Board</h2> | ||
| + | <p><font color=Gainsboro>The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.</font></p> | ||
| + | <h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">Check out the drawing board!</a></h2> | ||
| + | </div> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | <!-- Three --> | ||
| + | <section id="three" class="wrapper spotlight style3"> | ||
| + | <div class="inner"style=''width:60%;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory" class="image"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--UNebraska-Lincoln--iGEMAstock3.jpg" alt="" /></a> | ||
| + | <div class="content"> | ||
| + | <h2 class="major" style="font-size:50px">The Laboratory</h2> | ||
| + | <p><font color=Gainsboro>Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.</font></p> | ||
| + | <h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">Check out the Lab Bench!</a></h2> | ||
| + | </div> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | <!-- Two --> | ||
| + | <section id="three" class="wrapper alt spotlight style2"> | ||
| + | <div class="inner" style=''width:70%;"> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench" class="image"><img src="https://static.igem.org/mediawiki/2016/e/e9/T--UNebraska-Lincoln--iGEMAstock5.jpg" alt="" /></a> | ||
| + | <div class="content"> | ||
| + | <h2 class="major" style="font-size:50px">Beyond the Bench</h2> | ||
| + | <p><font color=Gainsboro>Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!</font></p> | ||
| + | <h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">Check out our Meeting Room!</a></h2> | ||
| + | </div> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | |||
| + | <!-- Four --> | ||
| + | <section id="four" class="wrapper alt style1"> | ||
| + | <div class="inner" style=''width:70%;"> | ||
| + | <h2 class="major" style="font-size:50px">Important:</h2> | ||
| + | <section class="features"> | ||
| + | <article> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="image"><img src="https://static.igem.org/mediawiki/2016/b/bd/T--UNebraska-Lincoln--sponsors.png" alt="" /></a> | ||
| + | <h3 class="major">Our Sponsors</h3> | ||
| + | <p><font color="silver">Our project and iGEM experience would not have been possible without our sponsors!</font></p> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="special">Learn more</a> | ||
| + | </article> | ||
| + | <article> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="image"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--UNebraska-Lincoln--thanks3.png" alt="" /></a> | ||
| + | <h3 class="major">Attributions</h3> | ||
| + | <p><font color="silver">Attribution of all external assistance, art, and web design.</font></p> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="special">Learn more</a> | ||
| + | </article> | ||
| + | <article> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="image"><img src="https://static.igem.org/mediawiki/2016/4/4b/T--UNebraska-Lincoln--iGEMAstock6.jpg" alt="" /></a> | ||
| + | <h3 class="major">Meet the Team!</h3> | ||
| + | <p><font color="silver">Our highly diverse of team of six undergraduates spans six majors and two countries.</font></p> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="special">Learn more</a> | ||
| + | </article> | ||
| + | <article> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="image"><img src="https://static.igem.org/mediawiki/2016/a/a6/T--UNebraska-Lincoln--SafetyStock.jpg" alt="" /></a> | ||
| + | <h3 class="major">Safety</h3> | ||
| + | <p><font color="silver">See the steps we took to ensure our own safety and the safety of our project.</font></p> | ||
| + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="special">Learn more</a> | ||
| + | </article> | ||
| + | </section> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | </section> | ||
| + | |||
| + | <!-- Footer --> | ||
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| + | </section> | ||
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Latest revision as of 02:44, 20 October 2016
Overview
The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.
As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!
The Drawing Board
The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.
Check out the drawing board!
The Laboratory
Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.
Check out the Lab Bench!
Beyond the Bench
Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!
Check out our Meeting Room!
Important:
Attributions
Attribution of all external assistance, art, and web design.
Learn more
Meet the Team!
Our highly diverse of team of six undergraduates spans six majors and two countries.
Learn more
Safety
See the steps we took to ensure our own safety and the safety of our project.
Learn more