![](https://static.igem.org/mediawiki/2016/b/bd/T--UNebraska-Lincoln--sponsors.png)
Our Sponsors
Our project and iGEM experience would not have been possible without our sponsors!
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a> | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a> | ||
+ | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a> | ||
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a> | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a> | ||
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a> | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a> | ||
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− | <a href="https://2016.igem.org/Team:UNebraska-Lincoln"> | + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions"> |
<img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/> | <img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/> | ||
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<section id="banner" > | <section id="banner" > | ||
<div class="inner" > | <div class="inner" > | ||
− | <h2 style="font-size: | + | <h2 style=" height: 90px; font-size:45px, padding-bottom: 30px">Knockout Nitrate</h2> |
<p><font color="Gainsboro">A synthetic biology project by the <a href="http://igem.unl.edu">University of Nebraska-Lincoln iGEM Team</a></font></p> | <p><font color="Gainsboro">A synthetic biology project by the <a href="http://igem.unl.edu">University of Nebraska-Lincoln iGEM Team</a></font></p> | ||
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<section id="one" class="wrapper spotlight style1"> | <section id="one" class="wrapper spotlight style1"> | ||
<div class="inner" > | <div class="inner" > | ||
− | <a class="image"><img src="https://static.igem.org/mediawiki/2016/9/90/T--UNebraska-Lincoln--iGEMAstock2.jpg" alt="" /></a> | + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Description" class="image"><img src="https://static.igem.org/mediawiki/2016/9/90/T--UNebraska-Lincoln--iGEMAstock2.jpg" alt="" /></a> |
<div class="content"> | <div class="content"> | ||
<h2 class="major" style="font-size:50px">Overview</h2> | <h2 class="major" style="font-size:50px">Overview</h2> | ||
− | <p align="center"><font color="Gainsboro">The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system. | + | <p align="center"><font color="Gainsboro">The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system. |
<p>As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy! | <p>As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy! | ||
</font></p></p> | </font></p></p> | ||
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<section id="three" class="wrapper alt spotlight style2"> | <section id="three" class="wrapper alt spotlight style2"> | ||
<div class="inner" style=''width:70%;"> | <div class="inner" style=''width:70%;"> | ||
− | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board" class="image"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--UNebraska-Lincoln--iGEMAstock4.jpg" alt="" /> | + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board" class="image"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--UNebraska-Lincoln--iGEMAstock4.jpg" alt="" /></a> |
<div class="content"> | <div class="content"> | ||
<h2 class="major" style="font-size:50px">The Drawing Board</h2> | <h2 class="major" style="font-size:50px">The Drawing Board</h2> | ||
<p><font color=Gainsboro>The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.</font></p> | <p><font color=Gainsboro>The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.</font></p> | ||
− | <a href | + | <h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">Check out the drawing board!</a></h2> |
− | </ | + | </div> |
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</section> | </section> | ||
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<h2 class="major" style="font-size:50px">The Laboratory</h2> | <h2 class="major" style="font-size:50px">The Laboratory</h2> | ||
<p><font color=Gainsboro>Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.</font></p> | <p><font color=Gainsboro>Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.</font></p> | ||
+ | <h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">Check out the Lab Bench!</a></h2> | ||
</div> | </div> | ||
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<h2 class="major" style="font-size:50px">Beyond the Bench</h2> | <h2 class="major" style="font-size:50px">Beyond the Bench</h2> | ||
<p><font color=Gainsboro>Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!</font></p> | <p><font color=Gainsboro>Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!</font></p> | ||
− | + | <h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">Check out our Meeting Room!</a></h2> | |
</div> | </div> | ||
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<div class="inner" style=''width:70%;"> | <div class="inner" style=''width:70%;"> | ||
<h2 class="major" style="font-size:50px">Important:</h2> | <h2 class="major" style="font-size:50px">Important:</h2> | ||
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− | <a href=" | + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="image"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--UNebraska-Lincoln--thanks3.png" alt="" /></a> |
<h3 class="major">Attributions</h3> | <h3 class="major">Attributions</h3> | ||
<p><font color="silver">Attribution of all external assistance, art, and web design.</font></p> | <p><font color="silver">Attribution of all external assistance, art, and web design.</font></p> | ||
− | <a href=" | + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="special">Learn more</a> |
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− | <a href=" | + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="image"><img src="https://static.igem.org/mediawiki/2016/a/a6/T--UNebraska-Lincoln--SafetyStock.jpg" alt="" /></a> |
<h3 class="major">Safety</h3> | <h3 class="major">Safety</h3> | ||
− | <p><font color="silver"> | + | <p><font color="silver">See the steps we took to ensure our own safety and the safety of our project.</font></p> |
− | <a href=" | + | <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="special">Learn more</a> |
</article> | </article> | ||
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The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.
As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!
The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.
Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.
Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!
Our project and iGEM experience would not have been possible without our sponsors!
Learn moreAttribution of all external assistance, art, and web design.
Learn moreOur highly diverse of team of six undergraduates spans six majors and two countries.
Learn moreSee the steps we took to ensure our own safety and the safety of our project.
Learn more