Difference between revisions of "Team:UNebraska-Lincoln"

 
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<h1>Project Desccription</h1>
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<h3>How did we choose our project?</h3>
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<p>We wanted our project to address a problem of significance on both global and local levels. Because Nebraska’s economy relies heavily on agriculture, a project devoted to an aspect of this industry was the obvious choice. Our objective is to reduce the nitrate levels in the waterways around Nebraska. Runoff from land treated with nitrate-containing fertilizers causes the accumulation of these compounds in waterways, where they are carried to the Gulf of Mexico. According to the National Oceanic and Atmospheric Administration, the Gulf of Mexico is the second-largest dead zone in the world, measuring approximately 5,500-6,500 square miles.</p>
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<p>A dead zone is a hypoxic area in a body of water where little marine life can survive. The excess nutrients in the water from fertilizers cause algal blooms at the surface. Because algae is not a good food source for other organisms in the ecosystem, it sinks and decomposes when it dies. The process of decomposition uses oxygen dissolved in the water, making the area uninhabitable for many species.</p>
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<p>We want to fix this problem by reducing nitrate levels in local rivers, addressing the problem at its source. We aim to engineer E. Coli to convert nitrate ions back into elemental nitrogen, a process called denitrification. Engineering E. coli to denitrify waters will involve introducing a nitrate-responsive promoter, corresponding bacterial ‘termination sequences’, and safety steps to ensure that the process can be controlled to the cells.</p>
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<h2> Welcome to the University of Nebraska-Lincoln 2016 iGEM team page! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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    background-image: url("https://static.igem.org/mediawiki/2016/4/42/T--UNebraska-Lincoln--NESkyline.png");
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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<h5> Styling your wiki </h5>
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    background-image: url("https://static.igem.org/mediawiki/2016/b/b7/T--UNebraska-Lincoln--knockoutnitrate.png");
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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html, body, div, span, applet, object, iframe,h1, h2, h3, h4, h5, h6, p, blockquote, pre,a, abbr, acronym, address, big, cite, code,del, dfn, em, font, img, ins, kbd, q, s, samp,small, strike, strong, sub, sup, tt, var,b, u, i, center,dl, dt, dd, ol, ul, li,fieldset, form, label, legend,table, caption, tbody, tfoot, thead, tr, th, td {margin: 0;padding: 0;border: 0;outline: 0;font-size: 100%;vertical-align: baseline;background: transparent;}body {line-height: 1;}ol, ul {list-style: none;}blockquote, q {quotes: none;}blockquote:before, blockquote:after,q:before, q:after {content: '';content: none;} /* remember to define focus styles! */ :focus {outline: 0;} /* remember to highlight inserts somehow! */ins {text-decoration: none;}del {text-decoration: line-through;} /* tables still need 'cellspacing="0"' in the markup */ table {border-collapse: collapse;border-spacing: 0;}
  
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<h5> Editing your wiki </h5>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
  
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln">
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<img src="https://static.igem.org/mediawiki/2016/0/02/T--UNebraska-Lincoln--home2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Home</span>
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<span class="sdt_descr"></span>
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<a  href="https://2016.igem.org/Team:UNebraska-Lincoln/Team">
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<span class="sdt_link"  >Team</span>
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<span class="sdt_descr"></span>
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</span>
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</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">
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<img src="https://static.igem.org/mediawiki/2016/1/11/T--UNebraska-Lincoln--Blueprints2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Blueprints</span>
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<span class="sdt_descr"></span>
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</span>
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</a>
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<div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/background">Background</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/modeling">Modeling</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/design">Design</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">
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<span class="sdt_link" style="">Laboratory</span>
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<span class="sdt_descr"></span>
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                            <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments">Experiments</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/lab_manual">Lab Notebook</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/safety_cases">Safety Cases</a>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">
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<img src="https://static.igem.org/mediawiki/2016/1/1a/T--UNebraska-Lincoln--beyondthebench2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_link">Outreach</span>
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<span class="sdt_descr"></span>
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                            <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
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                                                        <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions">
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<img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/>
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<span class="sdt_link">Thanks!</span>
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<span class="sdt_descr"></span>
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                <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions">Attributions</a>
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<h5>Tips</h5>
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<section id="banner" >
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<div class="inner" >
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<ul>
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<h2 style=" height: 90px; font-size:45px, padding-bottom: 30px">Knockout Nitrate</h2>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<p><font color="Gainsboro">A synthetic biology project by the <a href="http://igem.unl.edu">University of Nebraska-Lincoln iGEM Team</a></font></p>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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</div>
 
</div>
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</section>
  
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<!-- One -->
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<section id="one" class="wrapper spotlight style1">
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<div class="inner" >
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Description" class="image"><img src="https://static.igem.org/mediawiki/2016/9/90/T--UNebraska-Lincoln--iGEMAstock2.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">Overview</h2>
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<p align="center"><font color="Gainsboro">The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.
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<p>As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!
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</font></p></p>
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</div>
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</div>
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</section>
  
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<h5>Inspiration</h5>
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<section id="three" class="wrapper alt spotlight style2">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<div class="inner" style=''width:70%;">
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<ul>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board" class="image"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--UNebraska-Lincoln--iGEMAstock4.jpg" alt="" /></a>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<div class="content">
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<h2 class="major" style="font-size:50px">The Drawing Board</h2>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<p><font color=Gainsboro>The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.</font></p>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">Check out the drawing board!</a></h2>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h5> Uploading pictures and files </h5>
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<section id="three" class="wrapper spotlight style3">
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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<div class="inner"style=''width:60%;">
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory" class="image"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--UNebraska-Lincoln--iGEMAstock3.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">The Laboratory</h2>
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<p><font color=Gainsboro>Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">Check out the Lab Bench!</a></h2>
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</div>
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</div>
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</section>
  
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<!-- Two -->
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<section id="three" class="wrapper alt spotlight style2">
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<div class="inner" style=''width:70%;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench" class="image"><img src="https://static.igem.org/mediawiki/2016/e/e9/T--UNebraska-Lincoln--iGEMAstock5.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">Beyond the Bench</h2>
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<p><font color=Gainsboro>Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">Check out our Meeting Room!</a></h2>
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</div>
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</div>
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</section>
  
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<div class="button_click" onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">
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UPLOAD FILES
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<!-- Four -->
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<section id="four" class="wrapper alt style1">
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<div class="inner" style=''width:70%;">
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<h2 class="major" style="font-size:50px">Important:</h2>
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<section class="features">
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="image"><img src="https://static.igem.org/mediawiki/2016/b/bd/T--UNebraska-Lincoln--sponsors.png" alt="" /></a>
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<h3 class="major">Our Sponsors</h3>
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<p><font color="silver">Our project and iGEM experience would not have been possible without our sponsors!</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="special">Learn more</a>
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</article>
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="image"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--UNebraska-Lincoln--thanks3.png" alt="" /></a>
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<h3 class="major">Attributions</h3>
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<p><font color="silver">Attribution of all external assistance, art, and web design.</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="special">Learn more</a>
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</article>
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="image"><img src="https://static.igem.org/mediawiki/2016/4/4b/T--UNebraska-Lincoln--iGEMAstock6.jpg" alt="" /></a>
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<h3 class="major">Meet the Team!</h3>
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<p><font color="silver">Our highly diverse of team of six undergraduates spans six majors and two countries.</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="special">Learn more</a>
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</article>
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="image"><img src="https://static.igem.org/mediawiki/2016/a/a6/T--UNebraska-Lincoln--SafetyStock.jpg" alt="" /></a>
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<h3 class="major">Safety</h3>
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<p><font color="silver">See the steps we took to ensure our own safety and the safety of our project.</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="special">Learn more</a>
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</article>
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</section>
 
</div>
 
</div>
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</section>
  
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<footer class="footer">
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Latest revision as of 02:44, 20 October 2016

Solid State by HTML5 UP

Overview

The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.

As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!

The Drawing Board

The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.

Check out the drawing board!

The Laboratory

Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.

Check out the Lab Bench!

Beyond the Bench

Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!

Check out our Meeting Room!

Important:

Our Sponsors

Our project and iGEM experience would not have been possible without our sponsors!

Learn more

Attributions

Attribution of all external assistance, art, and web design.

Learn more

Meet the Team!

Our highly diverse of team of six undergraduates spans six majors and two countries.

Learn more

Safety

See the steps we took to ensure our own safety and the safety of our project.

Learn more