Difference between revisions of "Team:UNebraska-Lincoln/Safety"

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{{UNebraska-Lincoln}}
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<!DOCTYPE HTML>
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<!--
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Solid State by HTML5 UP
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span.reference{
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<div class="column full_size">
 
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<h5>Safe Project Design</h5>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<ul>
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</style>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<link rel="shortcut icon" href="../favicon.ico" type="image/x-icon"/>
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<h5>Safe Lab Work</h5>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/background">Background</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/modeling">Modeling</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/design">Design</a>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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</div>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">
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<span class="sdt_link" style="">Laboratory</span>
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<span class="sdt_descr"></span>
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                            <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments">Experiments</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/lab_manual">Lab Notebook</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/safety_cases">Safety Cases</a>
  
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<div class="column half_size">
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</div>
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<h5>Safe Shipment</h5>
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</span>
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</a>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">
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<img src="https://static.igem.org/mediawiki/2016/1/1a/T--UNebraska-Lincoln--beyondthebench2.png" alt=""/>
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<span class="sdt_link">Outreach</span>
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                            <div class="sdt_box">
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                                                        <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a>
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</div>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln">
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<img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/>
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<span class="sdt_link">Thanks!</span>
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<span class="sdt_descr"></span>
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                <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/attributions">Attributions</a>
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</div>
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</span>
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</a>
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</li>
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</ul>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
</div>
 
</div>
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</div>
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<section>
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<h2 class="major">Safety</h2>
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<font color="silver"><h1>Project environmental safety</h1></font><p><font color="silver">The design of our project required careful consideration as to the impact it might have on the environment. Our project would ideally be able to be released into a water system that was contaminated by high levels of nitrates. In its completed form it would be able to fully reduce the nitrates to nitrogen gas. With this in mind we needed to ensure that we didn’t transform a nitrate problem into an E. coli problem by introducing a strain of E. coli that would grow rampant.</font></p>
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<p><font color="silver">In order to prevent the E. coli from growing excessively, design and incorporation of a kill switch was a large part of our project. We wanted to create a kill switch that would allow our strain to grow successfully when there are high levels of nitrate ions for it to reduce, but that prevents it from growing when nitrate levels are low and the strain is no longer necessary. After consideration of several methods to achieve this desired effect, we settled on complementing an auxotrophic strain of E. coli with a gene that would only be expressed under high nitrate conditions. To accomplish this we looked to previous iGEM teams for inspiration and quickly discovered the Edinburugh 2009 team’s <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005 ">PyeaR BioBrick</a> that was shown to be a nitrate sensitive promoter for E. coli. We believed this promoter could be used with a serA gene to complement a ΔserA strain of E. coli that one of our <a href=https://2016.igem.org/Team:UNebraska-Lincoln/Attributions>advisers</a> had previously worked with.</font></p>
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<p><font color="silver">This work led to us designing and constructing the <a href="http://parts.igem.org/Part:BBa_K2086002">BioBrick BBa_K2086002</a>. Preliminary testing done by one of our <a href=https://2016.igem.org/Team:UNebraska-Lincoln/Attributions>advisors</a> showed that it is possible for this BioBrick to act as a successful kill-switch. </p>
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<font color="white"><h1>Lab Safety</h1></font>
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<p><font color="silver">In order to participate in this research project, all six of us completed university-wide lab safety training. During the course of our project we followed general lab safety procedures for working with materials that might potentially be biohazardous. While working in the lab we always wore lab coats, gloves, and safety glasses. Any time that cells were used or interacted with they were used in areas that were sanitized using a 70% ethanol solution and under a flame. Once finished, any material that came into contact with cells was placed in a bio-hazardous container to be autoclaved before being disposed of, or were rinsed in a bleach solution in order to be sanitized.</font></p>
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<h2 class="major">Want to see our project?</h2>
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board"><img src="https://static.igem.org/mediawiki/2016/a/ac/T--UNebraska-Lincoln--backtothedrawingboard.png"  style="width:60%;height:auto;" transform:scale(0.2)></a></center>
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Latest revision as of 02:49, 20 October 2016

<!DOCTYPE HTML> Elements - Solid State by HTML5 UP

Safety

Project environmental safety

The design of our project required careful consideration as to the impact it might have on the environment. Our project would ideally be able to be released into a water system that was contaminated by high levels of nitrates. In its completed form it would be able to fully reduce the nitrates to nitrogen gas. With this in mind we needed to ensure that we didn’t transform a nitrate problem into an E. coli problem by introducing a strain of E. coli that would grow rampant.

In order to prevent the E. coli from growing excessively, design and incorporation of a kill switch was a large part of our project. We wanted to create a kill switch that would allow our strain to grow successfully when there are high levels of nitrate ions for it to reduce, but that prevents it from growing when nitrate levels are low and the strain is no longer necessary. After consideration of several methods to achieve this desired effect, we settled on complementing an auxotrophic strain of E. coli with a gene that would only be expressed under high nitrate conditions. To accomplish this we looked to previous iGEM teams for inspiration and quickly discovered the Edinburugh 2009 team’s PyeaR BioBrick that was shown to be a nitrate sensitive promoter for E. coli. We believed this promoter could be used with a serA gene to complement a ΔserA strain of E. coli that one of our advisers had previously worked with.

This work led to us designing and constructing the BioBrick BBa_K2086002. Preliminary testing done by one of our advisors showed that it is possible for this BioBrick to act as a successful kill-switch.

Lab Safety

In order to participate in this research project, all six of us completed university-wide lab safety training. During the course of our project we followed general lab safety procedures for working with materials that might potentially be biohazardous. While working in the lab we always wore lab coats, gloves, and safety glasses. Any time that cells were used or interacted with they were used in areas that were sanitized using a 70% ethanol solution and under a flame. Once finished, any material that came into contact with cells was placed in a bio-hazardous container to be autoclaved before being disposed of, or were rinsed in a bleach solution in order to be sanitized.