Difference between revisions of "Team:UNebraska-Lincoln/Description"

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{{UNebraska-Lincoln}}
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<!DOCTYPE HTML>
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<!--
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Solid State by HTML5 UP
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html5up.net | @ajlkn
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Free for personal and commercial use under the CCA 3.0 license (html5up.net/license)
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<!--[if lte IE 9]><link rel="stylesheet" href="assets/css/ie9.css" /><![endif]-->
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</head>
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<body >
  
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<style>
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/* styling for the menu items */
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span.reference{
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position:fixed;
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left:10px;
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bottom:10px;
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font-size:12px;
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}
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span.reference a{
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color:#aaa;
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text-transform:uppercase;
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text-decoration:none;
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text-shadow:1px 1px 1px #000;
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margin-right:30px;
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span.reference a:hover{
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color:#ddd;
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ul.sdt_menu{
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margin-top:300px;
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                      padding-bottom: 10px;
  
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<div class="column full_size judges-will-not-evaluate">
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}
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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</div>
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/* when hovering on a menu item */
  
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<div class="column full_size">
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#NavBar{
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margin: 0;
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width:auto;
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size: 75%;
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background-image: url("https://static.igem.org/mediawiki/2016/4/4a/T--UNebraska-Lincoln--blue.png");
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padding-left: 200px;
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padding-top: 85px;
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height: 160px;}
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</style>
  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<!-- Page Wrapper -->
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<div id="page-wrapper">
  
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<link rel="shortcut icon" href="../favicon.ico" type="image/x-icon"/>
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<ul id="sdt_menu" class="sdt_menu">
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln">
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<img src="https://static.igem.org/mediawiki/2016/0/02/T--UNebraska-Lincoln--home2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Home</span>
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<span class="sdt_descr"></span>
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</span>
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</a>
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</li>
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<li style="background-color:#4c5c96;">
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<a  href="https://2016.igem.org/Team:UNebraska-Lincoln/Team">
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<img src="https://static.igem.org/mediawiki/2016/c/cb/T--UNebraska-Lincoln--TeamStock2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link"  >Team</span>
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<span class="sdt_descr"></span>
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</span>
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</a>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">
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<img src="https://static.igem.org/mediawiki/2016/1/11/T--UNebraska-Lincoln--Blueprints2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Blueprints</span>
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<span class="sdt_descr"></span>
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</span>
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</a>
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<div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/background">Background</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/modeling">Modeling</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/design">Design</a>
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<h5>What should this page contain?</h5>
 
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<ul>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<li>References and sources to document your research.</li>
 
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<li>Use illustrations and other visual resources to explain your project.</li>
 
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</ul>
 
  
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</div>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">
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<img src="https://static.igem.org/mediawiki/2016/a/a3/T--UNebraska-Lincoln--Laboratory2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link" style="">Laboratory</span>
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<span class="sdt_descr"></span>
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                            <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments">Experiments</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/lab_manual">Lab Notebook</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/safety_cases">Safety Cases</a>
  
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</div>
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</div>
  
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<div class="column full_size" >
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</span>
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</a>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">
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<img src="https://static.igem.org/mediawiki/2016/1/1a/T--UNebraska-Lincoln--beyondthebench2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Outreach</span>
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<span class="sdt_descr"></span>
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                            <div class="sdt_box">
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                                                        <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a>
  
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<h5>Advice on writing your Project Description</h5>
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</div>
  
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<p>
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</span>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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</a>
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</p>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln">
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<img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Thanks!</span>
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<span class="sdt_descr"></span>
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                <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/attributions">Attributions</a>
  
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<p>
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</div>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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</span>
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</p>
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</a>
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</li>
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</ul>
  
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</div>
 
</div>
 
</div>
  
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<div class="drawing board" style=" background-color: #2e3141; padding-top:50px;">
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                      <center><h2 style="height:100px; padding-top:5px"><font size="+10">Description</font></h2></center>
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                      <center><font color="silver">Our project, "Knockout Nitrate", is a developing project with the aim to use synthetic biology to balance the nitrogen cycle. </font></center>
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<p><center><font color="silver"><h2>Background</h2></font></center></p>
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<p><center><font color="silver">Nitrogen is a highly abundant element in the Earth’s atmosphere. Nitrogen is also essential for life. However, atmospheric nitrogen (N2) is not useful in living processes. Humans have developed processes to convert unreactive atmospheric nitrogen into a usable form of nitrogen such as Ammonia or Nitrate for plants and animals (reactive nitrogen). To maximize crop yield, many farmers add nitrogen based fertilizer to their crops. Oftentimes excess nitrogen in the soil is washed away by rain into other natural water systems. This nitrogen runoff can and does have devastating effects on the biodiversity in waterways.</font></center></p>
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<p><center><font color="silver">To read more background information about the nitrate problem, click the image below.</font></center></p>
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/background"><img src="https://static.igem.org/mediawiki/2016/6/67/T--UNebraska-Lincoln--background.png" style="width:40%; height:auto;" alt="image"/></a></center>
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<br><br>
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<p><center><font color="silver"><h2>Project Design</h2></font></center></p>
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<p><center><font color="silver">In order to combat the nitrate problem, we aimed to design a strain of <i>E. coli</i> capable of reducing these excess nitrate ions. By isolating a gene that codes of periplasmic nitrate reductases, we began the process of designing our synthetic organism.</font></center></p>
 +
<p><center><font color="silver">To read more information about the design of our system, click the image below.</font></center></p>
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/design"><img src="https://static.igem.org/mediawiki/2016/0/0f/T--UNebraska-Lincoln--design.png" style="width:40%; height:auto;" alt="image"/></a></center>
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<p><center><font color="silver"><h2>Experiments for Our System</h2></font></center></p>
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<p><center><font color="silver">Throughout the summer and into the fall, we implemented our design into the laboratory. We isolated and synthesized our periplasmic nitrate reductase genes, as well as developed a kill switch and safety-assurance cases</font></center></p>
 +
<p><center><font color="silver">To read more information on the application of our design, click the image below.</font></center></p>
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments"><img src="https://static.igem.org/mediawiki/2016/1/16/T--UNebraska-Lincoln--experiments.png" style="width:40%; height:auto;" alt="image"/></a></center>
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<p><center><font color="silver"><h2>Recharacterization of K381001</h2></font></center></p>
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<p><center><font color="silver">In the design of our project, we implemented a nitrate-sensitive kill switch. This took advantage of the fact that the yeaR promoter, originally submitted by the Edinburgh iGEM Team in 2009 (<a href="http://parts.igem.org/Part:BBa_K216005">K216005</a>) was active only in the presence of nitrate ion. We decided to begin our project by determining sensitivity of this promoter the concentrations of nitrate that would be present in waterways. To do this, we recharacterized the composite part <a href="http://parts.igem.org/Part:BBa_K381001">K381001</a>, a PyeaR-GFP nitrate sensor.  You can read more about our rechracterization of the PyeaR-GFP Biobrick <a href="http://parts.igem.org/Part:BBa_K381001:Experience#UNebraska-Lincoln_2016">Here</a>.</font></center></p>
  
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<div class="column half_size" >
 
  
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<h5>References</h5>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
  
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</div>
 
  
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<p></p>
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<style>.image{
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  position: relative;
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}
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.image a{
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  display: block;     
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  position: absolute;
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}</style>
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<section>
  
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<div class="column half_size" >
 
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<h5>Inspiration</h5>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
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<ul>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<!-- Footer -->
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<section id="footer">
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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<div class="inner">
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</ul>
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<h2 class="major">See the blueprints in action!</h2>
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</div>
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory"><img src="https://static.igem.org/mediawiki/2016/9/9c/T--UNebraska-Lincoln--tothelab.png"  style="width:60%;height:auto;" transform:scale(0.2)></a></center>
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</div>
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</section>
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</div>
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<!-- Scripts -->
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/skelmin?action=raw&amp;ctype=text/javascript"></script>
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/jqueryscrollex?action=raw&amp;ctype=text/javascript"></script>
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/util?action=raw&amp;ctype=text/javascript"></script>
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/main?action=raw&amp;ctype=text/javascript"></script>
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        <script type="text/javascript">
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Latest revision as of 02:55, 20 October 2016

<!DOCTYPE HTML> Elements - Solid State by HTML5 UP

Description

Our project, "Knockout Nitrate", is a developing project with the aim to use synthetic biology to balance the nitrogen cycle.

Background

Nitrogen is a highly abundant element in the Earth’s atmosphere. Nitrogen is also essential for life. However, atmospheric nitrogen (N2) is not useful in living processes. Humans have developed processes to convert unreactive atmospheric nitrogen into a usable form of nitrogen such as Ammonia or Nitrate for plants and animals (reactive nitrogen). To maximize crop yield, many farmers add nitrogen based fertilizer to their crops. Oftentimes excess nitrogen in the soil is washed away by rain into other natural water systems. This nitrogen runoff can and does have devastating effects on the biodiversity in waterways.

To read more background information about the nitrate problem, click the image below.

image


Project Design

In order to combat the nitrate problem, we aimed to design a strain of E. coli capable of reducing these excess nitrate ions. By isolating a gene that codes of periplasmic nitrate reductases, we began the process of designing our synthetic organism.

To read more information about the design of our system, click the image below.

image

Experiments for Our System

Throughout the summer and into the fall, we implemented our design into the laboratory. We isolated and synthesized our periplasmic nitrate reductase genes, as well as developed a kill switch and safety-assurance cases

To read more information on the application of our design, click the image below.

image

Recharacterization of K381001

In the design of our project, we implemented a nitrate-sensitive kill switch. This took advantage of the fact that the yeaR promoter, originally submitted by the Edinburgh iGEM Team in 2009 (K216005) was active only in the presence of nitrate ion. We decided to begin our project by determining sensitivity of this promoter the concentrations of nitrate that would be present in waterways. To do this, we recharacterized the composite part K381001, a PyeaR-GFP nitrate sensor. You can read more about our rechracterization of the PyeaR-GFP Biobrick Here.