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<p><center><font color="silver">In order to combat the nitrate problem, we aimed to design a strain of <i>E. coli</i> capable of reducing these excess nitrate ions. By isolating a gene that codes of periplasmic nitrate reductases, we began the process of designing our synthetic organism.</font></center></p> | <p><center><font color="silver">In order to combat the nitrate problem, we aimed to design a strain of <i>E. coli</i> capable of reducing these excess nitrate ions. By isolating a gene that codes of periplasmic nitrate reductases, we began the process of designing our synthetic organism.</font></center></p> | ||
<p><center><font color="silver">To read more information about the design of our system, click the image below.</font></center></p> | <p><center><font color="silver">To read more information about the design of our system, click the image below.</font></center></p> | ||
− | <center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/design"><img src="https://static.igem.org/mediawiki/2016/ | + | <center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/design"><img src="https://static.igem.org/mediawiki/2016/0/0f/T--UNebraska-Lincoln--design.png" style="width:40%; height:auto;" alt="image"/></a></center> |
+ | <p><center><font color="silver"><h2>Experiments for Our System</h2></font></center></p> | ||
+ | <p><center><font color="silver">Throughout the summer and into the fall, we implemented our design into the laboratory. We isolated and synthesized our periplasmic nitrate reductase genes, as well as developed a kill switch and safety-assurance cases</font></center></p> | ||
+ | <p><center><font color="silver">To read more information on the application of our design, click the image below.</font></center></p> | ||
+ | <center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments"><img src="https://static.igem.org/mediawiki/2016/1/16/T--UNebraska-Lincoln--experiments.png" style="width:40%; height:auto;" alt="image"/></a></center> | ||
+ | <p><center><font color="silver"><h2>Recharacterization of K381001</h2></font></center></p> | ||
+ | <p><center><font color="silver">In the design of our project, we implemented a nitrate-sensitive kill switch. This took advantage of the fact that the yeaR promoter, originally submitted by the Edinburgh iGEM Team in 2009 (<a href="http://parts.igem.org/Part:BBa_K216005">K216005</a>) was active only in the presence of nitrate ion. We decided to begin our project by determining sensitivity of this promoter the concentrations of nitrate that would be present in waterways. To do this, we recharacterized the composite part <a href="http://parts.igem.org/Part:BBa_K381001">K381001</a>, a PyeaR-GFP nitrate sensor. You can read more about our rechracterization of the PyeaR-GFP Biobrick <a href="http://parts.igem.org/Part:BBa_K381001:Experience#UNebraska-Lincoln_2016">Here</a>.</font></center></p> | ||
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Latest revision as of 02:55, 20 October 2016
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