Difference between revisions of "Team:USNA-Annapolis/Parts"

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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<img src="https://static.igem.org/mediawiki/2016/8/85/T--USNA-Annapolis--USNA_parts.png">
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h2 style="text-align:center;"><b><u> Some questions we asked ourselves were:</b></u></h2>
  
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<h3 style="text-align:center;"><b><u> What is your chassis organism?</b></u></h3>
  
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<p><i> E. Coli: </i></p>
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<li>UQ950</li>
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<li>WM3064</li>
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<li>Top10</li>
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</ul>
  
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<p> For our first year in the iGEM competition we stuck to cloning in E. coli. Future USNA teams may use the WM3064 strain to perform conjugation in more environmentally relevant chassis.</p>
  
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<p>Other:</p>
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<ul><li>Biocathode MCL (mixture of micro-organisms) </li></ul>
  
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<h3 style="text-align:center;"><b><u>Do you plan to experiment with any other organisms, besides your chassis?</b></u></h3>
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<ul><li>Tenderia</li>
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<li>Marinobacter</li>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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<h3 style="text-align:center;"><b><u>How will your project work?</b></u></h3>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<p>Describe the goal of your project: what is your engineered organism supposed to do?</p>
  
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<p>The idea behind our project is to create a working two part system that can: 1)detect the presence of conotoxins through the change in cell potential, and 2). to counter act this effect, (but first by designing it to illuminate before). This will be accomplished through the use of the ArcA and ArcB system, which is commonly used for respiratory regulation in many different organisms.</p>
  
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<h3 style="text-align:center;"><b><u>How would your project be used in the real world?</b></u></h3>
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<p> The primary purpose of our project is to protect the American Warfighter from biological harm on the battlefield, but also preventing the use of conotoxins as biological weapons of mass destruction.</p>
  
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<h3 style="text-align:center;"><b><u>What risks does your project pose at the laboratory stage? What actions are you taking to reduce those risks?</b></u></h3>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<div class="highlight">
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<groupparts>iGEM2016 Example</groupparts>
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<p>We use a few chemicals such as Ethidium Bromide which is extremely toxic, as well as some non-harmful strains of organisms, which can all pose some threat to people if not used/handled properly. Also, there are some risks with using potentially harmful lab equipment and tools, such as glassware, hot plates, and open flames, which can all potentially harm us.</p>
  
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<p>To minimize risk from our project we would include safety level 1 procedures: wearing gloves, sterilizing waste, being cautious of harmful chemicals and bio-hazards, and the use of lab coats as well as goggles.</p>
  
  
 
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Latest revision as of 03:05, 20 October 2016

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Some questions we asked ourselves were:

What is your chassis organism?

E. Coli:

  • UQ950
  • WM3064
  • Top10

For our first year in the iGEM competition we stuck to cloning in E. coli. Future USNA teams may use the WM3064 strain to perform conjugation in more environmentally relevant chassis.

Other:

  • Biocathode MCL (mixture of micro-organisms)

Do you plan to experiment with any other organisms, besides your chassis?

  • Tenderia
  • Marinobacter

How will your project work?

Describe the goal of your project: what is your engineered organism supposed to do?

The idea behind our project is to create a working two part system that can: 1)detect the presence of conotoxins through the change in cell potential, and 2). to counter act this effect, (but first by designing it to illuminate before). This will be accomplished through the use of the ArcA and ArcB system, which is commonly used for respiratory regulation in many different organisms.

How would your project be used in the real world?

The primary purpose of our project is to protect the American Warfighter from biological harm on the battlefield, but also preventing the use of conotoxins as biological weapons of mass destruction.

What risks does your project pose at the laboratory stage? What actions are you taking to reduce those risks?

We use a few chemicals such as Ethidium Bromide which is extremely toxic, as well as some non-harmful strains of organisms, which can all pose some threat to people if not used/handled properly. Also, there are some risks with using potentially harmful lab equipment and tools, such as glassware, hot plates, and open flames, which can all potentially harm us.

To minimize risk from our project we would include safety level 1 procedures: wearing gloves, sterilizing waste, being cautious of harmful chemicals and bio-hazards, and the use of lab coats as well as goggles.