Difference between revisions of "Team:UPMC-Paris/Proof"

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<h1>Proof of Concept</h1>
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<p><br>
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The first part of our project was to assemble our operator and pigments. We succed in creating the five part below.
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<br>BBa_K2180002:Fructose Operator
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<br>BBa_K2180001:Zinc Operator
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<br>BBa_K2180011:Arsenic Operator
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<br>BBa_K2180003:Sucrose Operator
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<br>BBa_K2180010:Cadmium Operator
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<br>We succeed to assemble those operators described above with <a href="http://parts.igem.org/Part:BBa_K2180006">the LacO promotor</a>, <a href="http://parts.igem.org/Part:BBa_K2180005">the blue pigment</a>, <a href="http://parts.igem.org/Part:BBa_K2180008">a terminator</a> and amplify them into <i>E. coli</i> (PSB1C3) to finally integrate those biobricks into <i>B. subtilis</i> (PBS1C)<br>
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Each of these part, are parts of a system of biosensing.
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You can find more information about these parts in the link below.
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<br><a href="http://parts.igem.org/Part:BBa_K2180002">Fructose Biobrick</a>
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<br><a href="http://parts.igem.org/Part:BBa_K2180001">Zinc Biobrick</a>
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<br><a href="http://parts.igem.org/Part:BBa_K2180011">Arsenic Biobrick</a>
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<br><a href="http://parts.igem.org/Part:BBa_K2180003">Sucrose Biobrick</a>
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<br><a href="http://parts.igem.org/Part:BBa_K2180010">Cadmium Biobrick</a>
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<br>
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Here are the pictures of the integrated blue pigment after putting the different factors (from the Reverse-API Gallery)
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<div class="image" id="Proof1">
  
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Latest revision as of 03:20, 20 October 2016