Difference between revisions of "Team:Alverno CA/Team"

 
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         <img alt="Alverno iGEM Logo" src=
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         <h2>Our Team</h2>
        "https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png"
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<h5>Alverno Heights Academy (High School) iGEM 2016 team is made up of students who are passionate about science.  The all-girl team ranges in age from 14 - 18 yrs old. Whether they are members of Alverno's Accelerated Honors Academy for Gifted Girls, or HS Seniors ready to go to college, their enthusiasm and curiosity are contagious.<br><br>
  
    <center>
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Alverno girls are no strangers to research, and have participated in Caltech's Solar Army SEAL Team, Summer SEAL Research at Caltech, and individual research projects.  With many awards in academics  and sports, they are a multi-talented group, who hail from Sierra Madre and neighboring cities, such as Pasadena,  Monrovia, San Gabriel, Altadena, Glendora, Rowland Heights, Temple City, and Arcadia.</h5>
        <h2>Interlab Study</h2>
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        <h3>Quantifying different sources of variation in gene expression<br> using
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        fluorescent transformed E. coli bacteria</h3>
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               <li>
 
               <li>
                 <img style="width:925px;margin-top:146px;" src="https://static.igem.org/mediawiki/2016/4/48/T--Alverno_CA--TeamGroup.jpg"  title="We're the Alverno_CA iGEM Team!"/>
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                 <img style="width:925px;margin-top:146px;" src="https://static.igem.org/mediawiki/2016/4/48/T--Alverno_CA--TeamGroup.jpg"  title="<font>We're the Alverno_CA iGEM Team!"/>
 
</li>
 
</li>
 
                 <li>
 
                 <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/8/8c/T--Alverno_CA--sam.png" title="<b>Sam C.</b><br>Head Coach; Graduate Student, Caltech, Bioengineering (Prof. Richard Murray); Former Co-Captain of William and Mary's iGEM 2014 Team"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/8/8c/T--Alverno_CA--sam.png" title="<b>Sam Clamons</b><br>Head Coach; Graduate Student, Caltech, Bioengineering (Prof. Richard Murray); Former Co-Captain of William and Mary's iGEM 2014 Team"/>
 
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  <li>
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    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/4/4e/T--Alverno_CA--cassowary.jpg" title="<b>Monica B.</b><br>"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/4/4e/T--Alverno_CA--cassowary.jpg" title="<b>Monica Barsever</b><br><br>PI"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/d/dd/T--Alverno_CA--teammarisa.png" title="<b>Marisa D.</b><br>HS Senior; National Honor Society Member (NHS, Math, English); John's Hopkins CTY top scoring student; numerous roles in Drama/Musical Theatre productions; Comedy Sportz (Improv) Team; classically trained ballerina"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/f/ff/T--Alverno_CA--squaremarisa.jpg" title="<b>Marisa D.</b><br>I have a repertoire of interpretive dances for the cell cycle, the unit circle, and photosynthesis.<br><br>HS Senior; National Honor Society Member (Science, NHS, Math, English); John's Hopkins CTY top scoring student; numerous roles in Drama/Musical Theatre productions; Comedy Sportz (Improv) Team; classically trained ballerina"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/3/36/T--Alverno_CA--teamasia.png" title="<b>Asia L.</b><br>HS Senior; Junior Service Project - Creating a STEM-based school program for low-income schools; National Honor Society (NHS)"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/5/57/T--Alverno_CA--asiasquarepipet.jpg" title="<b>Asia L.</b><br>Wikipedia says that I am the largest and most populous continent.<br><br>HS Senior; Junior Service Project - Creating a STEM-based school program for low-income schools; Science National Honor Society; National Honor Society (NHS)"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/8/87/T--Alverno_CA--teamalicia.png" title="<b>Alicia L.</b><br>HS Senior; CSF (California Scholarship Federation); NHS (National Honors Society); NAHS (National Arts Honors Society)"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/8/87/T--Alverno_CA--teamalicia.png" title="<b>Alicia L.</b><br>I like art and the color pink and animals of all kind. And science, of course. <br><br>HS Senior; CSF (California Scholarship Federation); Science National Honor Society; NHS (National Honors Society); NAHS (National Arts Honors Society)"/>
 
    </li>
 
    </li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/5/56/T--Alverno_CA--teamhanaa.png" title="<b>Hanaa Z.</b><br>HS Senior; Science Fair 2013 Honorable Mention; Participation Award at JPL Science Fair 2013; Junior Service Project - Creating a STEM-based school program for low-income schools"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/5/56/T--Alverno_CA--teamhanaa.png" title="<b>Hanaa Z.</b><br>Someday, I plan to create robotic replicas of human beings. I also enjoy the wonders of art and music. <br><br>HS Senior; Science Fair 2013 Honorable Mention; Participation Award at JPL Science Fair 2013; Science National Honor Society; English National Honor Society; California Scholarship Federation; Junior Service Project - Creating a STEM-based school program for low-income schools"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/9/97/T--Alverno_CA--teamlaura.png" title="<b>Laura C.</b><br>HS Junior: 2016 CTY Cogito Research Award Winner; Member of National Honor Society, National Art Society, Mu Alpha Theta, and CSF; Halloween Window Painting Contest winner"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/thumb/d/de/T--Alverno_CA--Laurasquare.jpg/600px-T--Alverno_CA--Laurasquare.jpg" title="<b>Laura C.</b><br>It's fun to solve mysteries. Well, generally speaking. <br><br>HS Junior: 2016 CTY Cogito Research Award Winner; Member of National Honor Society, Science National Honor Society, National Art Society, Mu Alpha Theta, and CSF; Halloween Window Painting Contest winner"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/c/cb/T--Alverno_CA--teamglorious.png" title="<b>Victoria C.</b><br>I am filled with an irresistible urge to do science. <br><br>HS Junior: Member of Alverno's Accelerated Honors Academy for Gifted Girls; 2016 CTY Cogito Research Award Winner; Numerous Math Contests (1st place); CM Advanced Level 10 in piano; Black belt in Taekwodo"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/c/cb/T--Alverno_CA--teamglorious.png" title="<b>Victoria C.</b><br>I am filled with an irresistible urge to do science. <br><br>HS Junior: Member of Alverno's Accelerated Honors Academy for Gifted Girls; 2016 CTY Cogito Research Award Winner; Numerous Math Contests (1st place); CM Advanced Level 10 in piano; Science National Honor Society; Black belt in Taekwodo"/>
 
    </li>
 
    </li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/1/1b/T--Alverno_CA--teamminjesus.png" title="<b>Minji K.</b><br>HS Junior; California Math Cup (2nd); Mu Alpha Theta (Math Honor Society)"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/1/1b/T--Alverno_CA--teamminjesus.png" title="<b>Minji K.</b><br>HS Junior; California Math Cup (2nd); Science National Honor Society; Mu Alpha Theta (Math Honor Society)"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/7/72/T--Alverno_CA--teamfeier.png" title="<b>Feier M.</b><br>HS Senior; CSF; Principal's Honor Roll, Level B Bel Canto; Lanting Cup calligraphy competition; an amateur writer and subtitles translator, member of track team, basketball team, and concert choir."/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/9/9f/T--Alverno_CA--feiersquare.jpg" title="<b>Feier M.</b><br>HS Senior; Science National Honor Society; CSF; Principal's Honor Roll, Level B Bel Canto; Lanting Cup calligraphy competition; an amateur writer and subtitles translator, member of track team, basketball team, and concert choir."/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/b/b9/T--Alverno_CA--teamevina.png" title="<b>Evina W.</b><br>HS Junior: 2016 CTY Cogito Research Award Winner; Alverno Math Team; Mu Alpha Theta (Math Honor Society), CSF, Principal's Honor Roll"/>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/1/17/T--Alverno_CA--evinasquare.jpg" title="<b>Evina W.</b><br>HS Junior: 2016 CTY Cogito Research Award Winner; Alverno Math Team; Science National Honor Society; Mu Alpha Theta (Math Honor Society), CSF, Principal's Honor Roll"/>
 
    </li>
 
    </li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/b/ba/T--Alverno_CA--teammelody.png" title="<b>Melody W.</b><br>
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    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/b/bf/T--Alverno_CA--melodysquare.jpg" title="<b>Melody W.</b><br>
I am a scientist of many passions, from music to art. I believe everything is connected in some way.<br><br>HS Junior: Member of Alverno's Accelerated Honors Academy for Gifted Girls; Youth Science Center STEM Essay Contest Winner; You Be the Chemist Regional Finalist; Pasadena Youth Orchestra: Wind Ensemble Award; Mu Alpha Theta"/>
+
I am a scientist of many passions, from music to art. I believe everything is connected in some way.<br><br>HS Junior: Member of Alverno's Accelerated Honors Academy for Gifted Girls; Science National Honor Society; Youth Science Center STEM Essay Contest Winner; You Be the Chemist Regional Finalist; Pasadena Youth Orchestra: Wind Ensemble Award; Mu Alpha Theta"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/9/9b/T--Alverno_CA--teambrianna.png" title="<b>Brianna B.</b><br>HS Sophomore; Science Best in Class (8th grade); Girl Scouts Bronze Award; Honor Roll"/>
+
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/9/9b/T--Alverno_CA--teambrianna.png" title="<b>Brianna B.</b><br> Hey! I love netfix, coffee, and need more sleep! <br> <br> HS Sophomore; Science Best in Class (8th grade); Girl Scouts Bronze Award; Principal's Honor Roll, CSF"/>
 
    </li>
 
    </li>
 
  <li>
 
  <li>
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  <li>
 
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/2/23/T--Alverno_CA--teamkatherine.png" title="<b>Katherine F.</b><br>
 
    <img style="height:302px;width:302px;" src="https://static.igem.org/mediawiki/2016/2/23/T--Alverno_CA--teamkatherine.png" title="<b>Katherine F.</b><br>
I spend my life laughing about band memes and and watching YouTube.<br><br>HS Freshman; Member of Alverno's Accelerated Honors Academy for Gifted Girls; Science Best in Class (8th grade)"/>
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I spend my life laughing about band memes and watching YouTube.<br><br>HS Freshman; Member of Alverno's Accelerated Honors Academy for Gifted Girls; Science Best in Class (8th grade)"/>
 
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    <h4>Summary:</h4>
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    <h5></h5>
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    <h5>As part of the 2016 iGEM Interlab Study, we tested 3 different
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    fluorescent protein expression plasmids, J23101, J23106, and J23117 with
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    positive and negative controls. We transformed the parts with iGEM's
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    transformation protocol. Our bacteria were cultured on a LB agar plate and
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    cultured in LB broth. To measure the differences in fluorescence expressed
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    by different strength plasmids, we used a plate reader with an OD600
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    reference point and FITC standard curve.</h5>
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<br><br>
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    <h4>Project Description:</h4>
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    <h5></h5>
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    <h5>The 2016 iGEM Interlab Study aims to compare results obtained by various
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    teams in order to quantify the expression of 5 different constructs using
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    fluorescence, which provides a useful insight into expression levels that
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    can be monitored without disrupting cells. Each device used in the study is
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    in the pSB1C3 plasmid backbone and are fluorescent protein expression
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    plasmids of different strength promoters. The devices we tested were
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    J23101, J23106, J23117 (Test Devices 1, 2, and 3). We used positive control
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    I20270 and negative control R0040. J23101, with a strong promoter, showed
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    the highest amount of fluorescence. J23117, with a weak promoter, showed
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    the lowest amount of fluorescence, and J23106, with the medium strength
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    promoter, showed fluorescence amounts between the other two. Our test
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    devices were inserted into DH5alpha E. coli, which were transformed
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    according to the iGEM transformation protocol. Our transformed cells were
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    plated on LB plates with chloramphenicol and incubated overnight at 37° C.
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    Two colonies were picked from each plate (with the exception of Test Device
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    3, with which there was only one) and were inoculated in 15mL test tubes.
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    These devices were diluted, and then measured in a plate reader with an
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    excitation wavelength of 35nm, according to iGEM’s measurement
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    protocol.</h5>
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<br><br>
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    <h4>Protocol:</h4>
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    <h5></h5>
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    <h5><a href=
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    "https://static.igem.org/mediawiki/2016/c/c5/InterLab_iGEM2016_Plate_Reader_Protocol_Updated_July.pdf">
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    Plate Reader Protocol</a>
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    </h5>
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    <h5><a href=
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    "https://static.igem.org/mediawiki/parts/6/67/IGEM_Registry_-_Transformation_Protocol.pdf">
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    Transformation Protocol</a>
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<br><br>
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    <h4>Results:</h4>
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    <h5></h5>
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        <center>
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            <table class="image">
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                    <td><img alt="Placeholder Image" src=
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                    "http://i.imgur.com/FcVrzL0.png" style="width:800px;">
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                <tr>
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                    <td class="caption">Graph of Absorbance (600) over a 6 hour
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                    time period</td>
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            <table class="image">
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                <tr>
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                    <td><img alt="Placeholder Image" src=
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                    "http://i.imgur.com/eKgjQDe.png" style="width:800px;">
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                    </td>
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                </tr>
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                <tr>
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                    <td class="caption">Graph of Fluorescence in AU over a 6
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                    hour time period</td>
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                </tr>
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            </table>
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        </center>
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        <center>
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            <table class="image">
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                <tr>
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                    <td><img alt="Placeholder Image" src=
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                    "http://i.imgur.com/oyXnJRE.png" style="width:800px;">
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                    </td>
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                </tr>
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                <tr>
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                    <td class="caption">Graph of Fluorescence/Absorbance in AU
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                    over a 6 hour time period</td>
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                </tr>
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            </table>
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        </center>
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        <table class="image">
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            <tr>
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                <td><img alt="Placeholder Image" src=
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                "http://i.imgur.com/lWwA42t.png" style="width:800px;">
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                <td class="caption">FITC Fluorescence standard curve. Row A had
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                pipetting errors and so is different from the other
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                curves.</td>
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            </tr>
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Latest revision as of 03:24, 20 October 2016

Alverno iGEM 2016

Our Team

Alverno Heights Academy (High School) iGEM 2016 team is made up of students who are passionate about science. The all-girl team ranges in age from 14 - 18 yrs old. Whether they are members of Alverno's Accelerated Honors Academy for Gifted Girls, or HS Seniors ready to go to college, their enthusiasm and curiosity are contagious.

Alverno girls are no strangers to research, and have participated in Caltech's Solar Army SEAL Team, Summer SEAL Research at Caltech, and individual research projects. With many awards in academics and sports, they are a multi-talented group, who hail from Sierra Madre and neighboring cities, such as Pasadena, Monrovia, San Gabriel, Altadena, Glendora, Rowland Heights, Temple City, and Arcadia.