Difference between revisions of "Team:UFAM-UEA Brazil/Design"

 
(3 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
{{UFAM-UEA_Brazil}}
+
{{UFAM-UEA_Brazil/CSS}}
 +
{{UFAM-UEA_Brazil/menu}}
 +
 
 +
 
 
<html>
 
<html>
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
 +
</head>
 +
<body>
 +
<div class="main-container">
 +
 +
<div class="main-content">
 +
 +
<h1 class="h-sectionT">Design</h1>
 +
<p>Greed is destroying natural resources in a fast track! Mercury is one of the major contaminants in Amazonian rivers. To enhance microbial performance in mercury bioremediation, we developed a very original system on both  molecular and physical design: a new set of strong promoters regulated by MerR, sensible to mercury, and one of them improved and increase the mer operon expression, check this promoters at:
  
 +
<a href="https://2016.igem.org/Team:UFAM-UEA_Brazil/Project/NewPromoters" target="blank" style="color:#40FF00" > new promoter</a>
  
−
<div class="Design">
+
with this we could increase the bioremediation removing 97% of the mercury in media when linked with the Operon Mer. The library is also important to offer promoter to several industrial processes and as guide to a great efficiency and could be suitable for other heavy metal operators, as a genetic referential.
 +
As second part of our design we built the first (real) bioreactor to treat mercury contaminated water and using our chassis carrying our synthetic genetic circuits! We had the awesome experience working with fermentation experts and see our system working. The interesting was to build it in a DIY way we had from 6L of media, with 10ug/mL, 69% of bioremediation in less than one day. Making it happen gave the ultimate proof of concept and was the first step to have this synbio system working out there, in the real world
  
−
<h1>To be continued</h1>
 
  
−
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aliquam lorem elit, molestie in nunc id, viverra iaculis augue. Praesent sed gravida lorem, vel sollicitudin turpis. Mauris efficitur ligula blandit lorem auctor lacinia. Etiam vitae justo nibh. Curabitur vehicula purus auctor lacus vestibulum feugiat. Pellentesque ex dui, pretium nec odio vitae, efficitur volutpat risus. Curabitur mollis tortor et hendrerit sodales. Nam id leo sit amet libero varius luctus. Nullam mollis mauris nec magna iaculis cursus. Nunc convallis fringilla nisl, ut scelerisque urna laoreet in. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed vitae ante viverra, luctus dolor et, rutrum tellus. Etiam in mollis turpis. Ut eu tellus id nunc congue cursus. Phasellus non leo ut ante ultricies sodales. Mauris congue lectus dui.</p>
+
<a href="https://2016.igem.org/Team:UFAM-UEA_Brazil/Project/ScaleUpBioreactor" target="blank" style="color:#40FF00" > Bioreactor</a>.</p>
  
 +
</div>
 +
 +
</div><!-- main-container -->
  
 +
</body>
  
 
</html>
 
</html>

Latest revision as of 03:42, 20 October 2016


Design

Greed is destroying natural resources in a fast track! Mercury is one of the major contaminants in Amazonian rivers. To enhance microbial performance in mercury bioremediation, we developed a very original system on both molecular and physical design: a new set of strong promoters regulated by MerR, sensible to mercury, and one of them improved and increase the mer operon expression, check this promoters at: new promoter with this we could increase the bioremediation removing 97% of the mercury in media when linked with the Operon Mer. The library is also important to offer promoter to several industrial processes and as guide to a great efficiency and could be suitable for other heavy metal operators, as a genetic referential. As second part of our design we built the first (real) bioreactor to treat mercury contaminated water and using our chassis carrying our synthetic genetic circuits! We had the awesome experience working with fermentation experts and see our system working. The interesting was to build it in a DIY way we had from 6L of media, with 10ug/mL, 69% of bioremediation in less than one day. Making it happen gave the ultimate proof of concept and was the first step to have this synbio system working out there, in the real world Bioreactor.