Difference between revisions of "Team:Alverno CA/Notebook"

 
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<head>
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<head>
 
<title>Alverno iGEM 2016</title>
 
<title>Alverno iGEM 2016</title>
 
  <link rel="icon" href="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png">
 
  <link rel="icon" href="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png">
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<br>
 
<br>
 
<br>
 
<br>
−
<center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"</center>
 
 
<h2><center>Notebook</center></h2>
 
<h2><center>Notebook</center></h2>
  
−
<left><h2>Week of June 6th (Limited amount of notes taken)</h2></left>
+
<center>
−
<p>- Lots of Designing Parts and Primers</p>
+
<img class="icons"src="https://static.igem.org/mediawiki/2016/a/ac/T--Alverno_CA--labnotbook.jpg" style= width:500px>
−
<p>- Wiki Work</p>
+
<center>
  
−
<left><h2>Week of June 13th:</h2></left>
+
<h3>Week of June 6th (Limited amount of notes taken)</h3>
−
<p>- Cleaning Restriction Digest and Ligation Done</p>
+
<h5>- Lots of Designing Parts and Primers</h5>
−
<p>- Ampicillin plates made</p>
+
<h5>- Wiki Work</h5>
−
<p>- Ampicillin stock made</p>
+
−
<p>- Transform RFP plasmid</p>
+
−
<p>- Design Parts and Primers</p>
+
−
<p>- Wiki Work</p>
+
  
−
<left><h2>Week of June 20th</h2></left>
+
<h3>Week of June 13th:</h3>
−
<p>- Transformation Done (probably GFP)</p>
+
<h5>- Cleaning Restriction Digest and Ligation Done</h5>
−
<p>- RFP and GRP colonies</p>
+
<h5>- Ampicillin plates made</h5>
−
<p>- PCR for RFP Coding Gene</p>
+
<h5>- Ampicillin stock made</h5>
−
<p>- Design Parts and Primers</p>
+
<h5>- Transform RFP plasmid</h5>
−
<p>- Wiki Work</p>
+
<h5>- Design Parts and Primers</h5>
 +
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of June 27th</h2></left>
+
<h3>Week of June 20th</h3>
−
<p>- PCR Done for promoters, terminators, some UC parts</p>
+
<h5>- Transformation Done (probably GFP)</h5>
−
<p>- Primer Dilutions Done</p>
+
<h5>- RFP and GRP colonies</h5>
−
<p>- Gel Run with PCR product from Week of June 20th (RFP)</p>
+
<h5>- PCR for RFP Coding Gene</h5>
−
<p>- Miniprep kit for RFP Bba</p>
+
<h5>- Design Parts and Primers</h5>
−
<p>- TBE Buffer 10x and 1x made</p>
+
<h5>- Wiki Work</h5>
−
<p>- PCR Purification done on Promoters and Terminators</p>
+
−
<p>- Wiki Work</p>
+
  
−
<left><h2>Week of July 4th</h2></left>
+
<h3>Week of June 27th</h3>
−
<p>- PCR of Vectors, some UC parts</p>
+
<h5>- PCR Done for promoters, terminators, some UC parts</h5>
−
<p>- Primer Dilutions</p>
+
<h5>- Primer Dilutions Done</h5>
−
<p>- Gel Run UC16-18 and UC2 (UC18 ran twice)</p>
+
<h5>- Gel Run with PCR product from Week of June 20th (RFP)</h5>
−
<p>- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)</p>
+
<h5>- Miniprep kit for RFP Bba</h5>
−
<p>- Plate Reader</p>
+
<h5>- TBE Buffer 10x and 1x made</h5>
−
<p>- Wiki Work</p>
+
<h5>- PCR Purification done on Promoters and Terminators</h5>
 +
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of July 11th</h2></left>
+
<h3>Week of July 4th</h3>
 +
<h5>- PCR of Vectors, some UC parts</h5>
 +
<h5>- Primer Dilutions</h5>
 +
<h5>- Gel Run UC16-18 and UC2 (UC18 ran twice)</h5>
 +
<h5>- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)</h5>
 +
<h5>- Plate Reader</h5>
 +
<h5>- Wiki Work</h5>
 +
 
 +
<h3>Week of July 11th</h3>
 
<img src="https://static.igem.org/mediawiki/2016/4/44/T--Alverno_CA--Screenshot_2016-10-15_09.24.58.png" alt="iGEM" style="max-width:100%; max-height:100%">
 
<img src="https://static.igem.org/mediawiki/2016/4/44/T--Alverno_CA--Screenshot_2016-10-15_09.24.58.png" alt="iGEM" style="max-width:100%; max-height:100%">
−
<p>- Learn how to use Benchling</p>
+
<h5>- Learn how to use Benchling</h5>
−
<p>- Primers Diluted</p>
+
<h5>- Primers Diluted</h5>
 
<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.05.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.05.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Parts Diluted </p>
+
<h5>- Parts Diluted</h5>
−
<p>- PCR done on P1ab, P2ab, T3ab, T4ab</p>
+
<h5>- PCR done on P1ab, P2ab, T3ab, T4ab</h5>
−
<p>- Gel Run on P1ab, P2ab, T3ab, T4ab</p>
+
<h5>- Gel Run on P1ab, P2ab, T3ab, T4ab</h5>
−
<p>- PCR Clean up done on P1ab, P2ab, T3ab, T4ab</p>
+
<h5>- PCR Clean up done on P1ab, P2ab, T3ab, T4ab</h5>
−
<p>- Chloramphenicol plates made</p>
+
<h5>- Chloramphenicol plates made</h5>
−
<p>- Wiki Work
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of July 18th</h2></left>
+
<h3>Week of July 18th</h3>
−
<p>- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)</p>
+
<h5>- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)</h5>
 
<img src="https://static.igem.org/mediawiki/2016/d/d5/T--Alverno_CA--Screenshot_2016-10-15_09.05.53.png" alt="iGEM" style="width:30%; height:30%">
 
<img src="https://static.igem.org/mediawiki/2016/d/d5/T--Alverno_CA--Screenshot_2016-10-15_09.05.53.png" alt="iGEM" style="width:30%; height:30%">
−
<p>- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d</p>
+
<h5>- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d</h5>
 
<img src="https://static.igem.org/mediawiki/2016/5/5d/T--Alverno_CA--Screenshot_2016-10-15_09.06.03.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/5/5d/T--Alverno_CA--Screenshot_2016-10-15_09.06.03.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Transformation Done</p>
+
<h5>- Transformation Done</h5>
−
<p>- Chloramphenicol plates made</p>
+
<h5>- Chloramphenicol plates made</h5>
−
<p>- Parts dilutions Done</p>
+
<h5>- Parts dilutions Done</h5>
−
<p>- PCR of GG Assemblies</p>
+
<h5>- PCR of GG Assemblies</h5>
−
<p>- PCR of UC3a-UC9a</p>
+
<h5>- PCR of UC3a-UC9a</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of July 25th</h2></left>
+
<h3>Week of July 25th</h3>
 
<img src="https://static.igem.org/mediawiki/2016/4/4f/T--Alverno_CA--Screenshot_2016-10-15_09.06.12.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/4/4f/T--Alverno_CA--Screenshot_2016-10-15_09.06.12.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Set up Plate reader</p>
+
<h5>- Set up Plate reader</h5>
−
<p>- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts</p>
+
<h5>- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts</h5>
−
<p>- PCR Purification of UC3-9, V1a & V2a</p>
+
<h5>- PCR Purification of UC3-9, V1a & V2a</h5>
 
<img src="https://static.igem.org/mediawiki/2016/d/d9/T--Alverno_CA--Screenshot_2016-10-15_09.06.21.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/d/d9/T--Alverno_CA--Screenshot_2016-10-15_09.06.21.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Picked Colonies</p>
+
<h5>- Picked Colonies</h5>
−
<p>- Transformation Protocol for GG1-8</p>
+
<h5>- Transformation Protocol for GG1-8</h5>
−
<p>- Gel made & Gel made w/ SYBR safe</p>
+
<h5>- Gel made & Gel made w/ SYBR safe</h5>
−
<p>- PCR of GG Assemblies</p>
+
<h5>- PCR of GG Assemblies</h5>
−
<p>- PCR for V1a and V2a, UC7-9</p>
+
<h5>- PCR for V1a and V2a, UC7-9</h5>
−
<p>- Dilutions Done</p>
+
<h5>- Dilutions Done</h5>
−
<p>- Gel Run for UC7-9</p>
+
<h5>- Gel Run for UC7-9</h5>
−
<p>- Golden Gate Assembly GG9-16</p>
+
<h5>- Golden Gate Assembly GG9-16</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of August 1st</h2></left>
+
<h3>Week of August 1st</h3>
−
<p>- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23</p>
+
<h5>- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23</h5>
−
<p>- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12</p>
+
<h5>- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12</h5>
−
<p>- PCR Purification for UC7-8</p>
+
<h5>- PCR Purification for UC7-8</h5>
−
<p>- Parts Dilutions done UC2 & UC3-6 & UC7-8</p>
+
<h5>- Parts Dilutions done UC2 & UC3-6 & UC7-8</h5>
−
<p>- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23</p>
+
<h5>- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23</h5>
 
<img src="https://static.igem.org/mediawiki/2016/d/d6/T--Alverno_CA--Screenshot_2016-10-15_09.06.31.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/d/d6/T--Alverno_CA--Screenshot_2016-10-15_09.06.31.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Fixed  Thermocycler</p>
+
<h5>- Fixed  Thermocycler</h5>
−
<p>- Gels Made</p>
+
<h5>- Gels Made</h5>
−
<p>- Agar Plates made w/ Kan</p>
+
<h5>- Agar Plates made w/ Kan</h5>
−
<p>- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26</p>
+
<h5>- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26</h5>
 
<img src="https://static.igem.org/mediawiki/2016/c/c2/T--Alverno_CA--Screenshot_2016-10-15_09.06.45.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/c/c2/T--Alverno_CA--Screenshot_2016-10-15_09.06.45.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Transformation with GG12</p>
+
<h5>- Transformation with GG12</h5>
−
<p>- Ludox Calibration</p>
+
<h5>- Ludox Calibration</h5>
−
<p>- Test Fluorescence of Plasmids</p>
+
<h5>- Test Fluorescence of Plasmids</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of August 8th</h2></left>
+
<h3>Week of August 8th</h3>
 
<img src="https://static.igem.org/mediawiki/2016/5/53/T--Alverno_CA--Screenshot_2016-10-15_09.06.52.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/5/53/T--Alverno_CA--Screenshot_2016-10-15_09.06.52.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Cell Measurement</p>
+
<h5>- Cell Measurement</h5>
 
<img src="https://static.igem.org/mediawiki/2016/0/0e/T--Alverno_CA--Screenshot_2016-10-15_09.07.03.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/0/0e/T--Alverno_CA--Screenshot_2016-10-15_09.07.03.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR</p>
+
<h5>- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR</h5>
−
<p>- PCR Check for GG24-26 & GG27-28</p>
+
<h5>- PCR Check for GG24-26 & GG27-28</h5>
−
<p>- Made Gel</p>
+
<h5>- Made Gel</h5>
−
<p>- Transformation with GG24-26 & GG27-28</p>
+
<h5>- Transformation with GG24-26 & GG27-28</h5>
−
<p>- Golden Gate Assembly GG27-28</p>
+
<h5>- Golden Gate Assembly GG27-28</h5>
−
<p>- Colony PCR Check of GG24-26</p>
+
<h5>- Colony PCR Check of GG24-26</h5>
−
<p>- Primer Dilutions</p>
+
<h5>- Primer Dilutions</h5>
−
<p>- Plasmid DNA Purification</p>
+
<h5>- Plasmid DNA Purification</h5>
−
<p>- LB Kan Broth</p>
+
<h5>- LB Kan Broth</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of August 15th</h2></left>
+
<h3>Week of August 15th</h3>
 
<img src="https://static.igem.org/mediawiki/2016/5/5c/T--Alverno_CA--Screenshot_2016-10-15_09.07.17.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/5/5c/T--Alverno_CA--Screenshot_2016-10-15_09.07.17.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Lab Meet-up with UCL/LC/CV</p>
+
<h5>- Lab Meet-up with UCL/LC/CV</h5>
−
<p>- Colony PCR Check for GG libraries 27-28</p>
+
<h5>- Colony PCR Check for GG libraries 27-28</h5>
−
<p>- Gels Made</p>
+
<h5>- Gels Made</h5>
−
<p>- Gel Run for GG Library 28</p>
+
<h5>- Gel Run for GG Library 28</h5>
−
<p>- Grow Cultures GG27</p>
+
<h5>- Grow Cultures GG27</h5>
 
<img src="https://static.igem.org/mediawiki/2016/0/0c/T--Alverno_CA--Screenshot_2016-10-15_09.07.26.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/0/0c/T--Alverno_CA--Screenshot_2016-10-15_09.07.26.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Learn Python</p>
+
<h5>- Learn Python</h5>
−
<p>- Check Fluorescence for GG28 & GG27</p>
+
<h5>- Check Fluorescence for GG28 & GG27</h5>
−
<p>- PCR UC14a</p>
+
<h5>- PCR UC14a</h5>
−
<p>- Purification of Plasmid DNA</p>
+
<h5>- Purification of Plasmid DNA</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of August 22nd</h2></left>
+
<h3>Week of August 22nd</h3>
 
<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.07.34.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.07.34.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36</p>
+
<h5>- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36</h5>
−
<p>- Gels Made</p>
+
<h5>- Gels Made</h5>
−
<p>- PCR UC13a and UC15a (Done twice) & UC1</p>
+
<h5>- PCR UC13a and UC15a (Done twice) & UC1</h5>
−
<p>- PCR Purification of UC14 & UC13/15 & UC1</p>
+
<h5>- PCR Purification of UC14 & UC13/15 & UC1</h5>
−
<p>- Golden Gate Assembly for GG29-36</p>
+
<h5>- Golden Gate Assembly for GG29-36</h5>
−
<p>- PCR Check for GG29-36</p>
+
<h5>- PCR Check for GG29-36</h5>
−
<p>- LB Kan Broth Made</p>
+
<h5>- LB Kan Broth Made</h5>
−
<p>- Dilution of UC1</p>
+
<h5>- Dilution of UC1</h5>
−
<p>- Control run in Plate Reader</p>
+
<h5>- Control run in Plate Reader</h5>
 
<img src="https://static.igem.org/mediawiki/2016/6/69/T--Alverno_CA--Screenshot_2016-10-15_09.07.46.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/6/69/T--Alverno_CA--Screenshot_2016-10-15_09.07.46.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of August 29th</h2></left>
+
<h3>Week of August 29th</h3>
 
<img src="https://static.igem.org/mediawiki/2016/9/9b/T--Alverno_CA--Screenshot_2016-10-15_09.07.54.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/9/9b/T--Alverno_CA--Screenshot_2016-10-15_09.07.54.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Make Gels</p>
+
<h5>- Make Gels</h5>
−
<p>- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a</p>
+
<h5>- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a</h5>
−
<p>- Golden Gate Assembly GG37-52 & GG53-60</p>
+
<h5>- Golden Gate Assembly GG37-52 & GG53-60</h5>
−
<p>- PCR Check GG37-52 & GG53-60</p>
+
<h5>- PCR Check GG37-52 & GG53-60</h5>
−
<p>- PCR Check Gel run for GG37-52</p>
+
<h5>- PCR Check Gel run for GG37-52</h5>
−
<p>- PCR CDS1a & CDS2a</p>
+
<h5>- PCR CDS1a & CDS2a</h5>
−
<p>- PCR Purification of CDS1a & CDS2a</p>
+
<h5>- PCR Purification of CDS1a & CDS2a</h5>
 
<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Alverno_CA--Screenshot_2016-10-15_09.08.02.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Alverno_CA--Screenshot_2016-10-15_09.08.02.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Talk with iGEM Peshawar</p>
+
<h5>- Talk with iGEM Peshawar</h5>
−
<p>- LB Kan Plates Made</p>
+
<h5>- LB Kan Plates Made</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of September 5th</h2></left>
+
<h3>Week of September 5th</h3>
 
<img src="https://static.igem.org/mediawiki/2016/a/ae/T--Alverno_CA--Screenshot_2016-10-15_09.08.11.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/a/ae/T--Alverno_CA--Screenshot_2016-10-15_09.08.11.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- iGEM Team Meeting</p>
+
<h5>- iGEM Team Meeting</h5>
−
<p>- PCR Purification of CDS1a & CDS2a and UC1</p>
+
<h5>- PCR Purification of CDS1a & CDS2a and UC1</h5>
−
<p>- <p>- Transformation was done with GG53-60</p>
+
<h5>- Transformation was done with GG53-60</h5>
−
<p>- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64</p>
+
<h5>- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64</h5>
−
<p>- Gels made</p>
+
<h5>- Gels made</h5>
−
<p>- Dilution of CDS1a & CDS2a</p>
+
<h5>- Dilution of CDS1a & CDS2a</h5>
−
<p>- Golden Gate Assembly GG61-64</p>
+
<h5>- Golden Gate Assembly GG61-64</h5>
−
<p>- PCR of UC1</p>
+
<h5>- PCR of UC1</h5>
−
<p>- LB Kan Plates Made</p>
+
<h5>- LB Kan Plates Made</h5>
−
<p>- PCR Check for GG61-64</p>
+
<h5>- PCR Check for GG61-64</h5>
 
<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.20.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.20.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- TBE 10x Buffer</p>
+
<h5>- TBE 10x Buffer</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of September 12th</h2></left>
+
<h3>Week of September 12th</h3>
 
<img src="https://static.igem.org/mediawiki/2016/f/f2/T--Alverno_CA--Screenshot_2016-10-15_09.08.27.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/f/f2/T--Alverno_CA--Screenshot_2016-10-15_09.08.27.png" alt="iGEM" style="width:20%; height:20%">
−
 
+
<h5>- Talked with UCL iGEM Team</h5>
−
<p>- Talked with UCL iGEM Team</p>
+
<h5>- LV-LC-CV meet-up</h5>
−
<p>- LV-LC-CV meet-up</p>
+
<h5>- Google Hangouts with Lambert Georgia iGEM team</h5>
−
<p>- Google Hangouts with Lambert Georgia iGEM team</p>
+
 
<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.35.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.35.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Dilution of UC1a</p>
+
<h5>- Dilution of UC1a</h5>
−
<p>- Transformation of GG29-36</p>
+
<h5>- Transformation of GG29-36</h5>
−
<p>- Golden Gate Assembly GG65-72</p>
+
<h5>- Golden Gate Assembly GG65-72</h5>
−
<p>- PCR GG61-64</p>
+
<h5>- PCR GG61-64</h5>
−
<p>- PCR Check GG61-64</p>
+
<h5>- PCR Check GG61-64</h5>
−
<p>- TX-TL of GFP (+ control)</p>
+
<h5>- TX-TL of GFP (+ control)</h5>
 
<img src="https://static.igem.org/mediawiki/2016/0/04/T--Alverno_CA--Screenshot_2016-10-15_09.08.42.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/0/04/T--Alverno_CA--Screenshot_2016-10-15_09.08.42.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Outreach workshop with Middle school</p>
+
<h5>- Outreach workshop with Middle school</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of September 19th</h2></left>
+
<h3>Week of September 19th</h3>
−
<p>- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE</p>
+
<h5>- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE</h5>
 
<img src="https://static.igem.org/mediawiki/2016/4/4a/T--Alverno_CA--Screenshot_2016-10-15_09.08.50.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/4/4a/T--Alverno_CA--Screenshot_2016-10-15_09.08.50.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Run Gel for GG61-64 (done twice) and GG65-72</p>
+
<h5>- Run Gel for GG61-64 (done twice) and GG65-72</h5>
−
<p>- LB Kan Plates Made</p>
+
<h5>- LB Kan Plates Made</h5>
−
<p>- Gels Made</p>
+
<h5>- Gels Made</h5>
−
<p>- TX-TL GFP Re-run</p>
+
<h5>- TX-TL GFP Re-run</h5>
−
<p>- Gel Run for UC1 and GG61-64</p>
+
<h5>- Gel Run for UC1 and GG61-64</h5>
−
<p>- Control Test ran using only the ladder for Gels</p>
+
<h5>- Control Test ran using only the ladder for Gels</h5>
−
<p>- TX-TL W & M plasmids</p>
+
<h5>- TX-TL W & M plasmids</h5>
−
<p>- PCR U3a</p>
+
<h5>- PCR U3a</h5>
−
<p>- Dilution of Primers</p>
+
<h5>- Dilution of Primers</h5>
 
<img src="https://static.igem.org/mediawiki/2016/3/3f/T--Alverno_CA--Screenshot_2016-10-15_09.09.01.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/3/3f/T--Alverno_CA--Screenshot_2016-10-15_09.09.01.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of September 26th</h2></left>
+
<h3>Week of September 26th</h3>
−
<p>- PCR Purification of V3a</p>
+
<h5>- PCR Purification of V3a</h5>
−
<p>- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94</p>
+
<h5>- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94</h5>
−
<p>- PCR Check GG73-74 and GG75-82</p>
+
<h5>- PCR Check GG73-74 and GG75-82</h5>
−
<p>- Dilution V3a</p>
+
<h5>- Dilution V3a</h5>
−
<p>- Run Gel V3a</p>
+
<h5>- Run Gel V3a</h5>
−
<p>- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74</p>
+
<h5>- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74</h5>
−
<p>- TX-TL re-run for W & M plasmids</p>
+
<h5>- TX-TL re-run for W & M plasmids</h5>
−
<p>- Colony PCR GG61-64 and GG65-72</p>
+
<h5>- Colony PCR GG61-64 and GG65-72</h5>
 
<img src="https://static.igem.org/mediawiki/2016/c/c6/T--Alverno_CA--Screenshot_2016-10-15_09.09.08.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/c/c6/T--Alverno_CA--Screenshot_2016-10-15_09.09.08.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- LB Chloramphenicol plates & LB Kanamycin plates</p>
+
<h5>- LB Chloramphenicol plates & LB Kanamycin plates</h5>
 
<img src="https://static.igem.org/mediawiki/2016/6/65/T--Alverno_CA--Screenshot_2016-10-15_09.09.17.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/6/65/T--Alverno_CA--Screenshot_2016-10-15_09.09.17.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Wiki Work
+
<h5>- Wiki Work</h5>
  
−
<left><h2>Week of October 3rd</h2></left>
+
<h3>Week of October 3rd</h3>
 
<img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--Screenshot_2016-10-15_09.09.24.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--Screenshot_2016-10-15_09.09.24.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Middle School Visit</p>
+
<h5>- Middle School Visit</h5>
−
<p>- LB Broth made</p>
+
<h5>- LB Broth made</h5>
−
<p>- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</p>
+
<h5>- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</h5>
−
<p>- Dilution V3a</p>
+
<h5>- Dilution V3a</h5>
−
<p>- PCR Check GG83-90</p>
+
<h5>- PCR Check GG83-90</h5>
−
<p>- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</p>
+
<h5>- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</h5>
−
<p>- TX-TL re-run for W & M plasmids w/ Replicates</p>
+
<h5>- TX-TL re-run for W & M plasmids w/ Replicates</h5>
−
<p>- Gels Made</p>
+
<h5>- Gels Made</h5>
−
<p>- Mini prep GG28 1-5</p>
+
<h5>- Mini prep GG28 1-5</h5>
−
<p>- Make TBE 1x</p>
+
<h5>- Make TBE 1x</h5>
 
<img src="https://static.igem.org/mediawiki/2016/9/99/T--Alverno_CA--Screenshot_2016-10-15_09.09.30.png" alt="iGEM" style="width:20%; height:20%">
 
<img src="https://static.igem.org/mediawiki/2016/9/99/T--Alverno_CA--Screenshot_2016-10-15_09.09.30.png" alt="iGEM" style="width:20%; height:20%">
−
<p>- Gel Run P1a-P4a, T1a-T4a, P1ab, P2ab, T3ab, T4ab & GG95-102</p>
+
<h5>- Gel Run P1a-P4a, T1a-T4a, P1ab, P2ab, T3ab, T4ab & GG95-102</h5>
−
<p>- Primers Diluted</p>
+
<h5>- Primers Diluted</h5>
−
<p>- Golden Gate GG103 & GG104 & GG85-102 & GG105-112</p>
+
<h5>- Golden Gate GG103 & GG104 & GG85-102 & GG105-112</h5>
−
<p>- Transformation GG95-102 (done twice)</p>
+
<h5>- Transformation GG95-102 (done twice)</h5>
−
<p>- Testing Fluorescence on GG28 1-5 w/ Plate Reader</p>
+
<h5>- Testing Fluorescence on GG28 1-5 w/ Plate Reader</h5>
−
<p>- Wiki Work</p>
+
<h5>- Wiki Work</h5>
−
<left><h2>Week of October 10th</h2></left>
+
−
<p>- Golden Gate GG113-114</p>
+
−
<p>- Transformation GG103 & 104, GG105-112</p>
+
−
<p>- LB Chloramphenicol plates made</p>
+
−
<p>- PCR Purification of UC13a-UC15a</p>
+
−
<p>- Transformation done GG113-114</p>
+
−
<p>- LB Broth Made</p>
+
−
<p>- Mini Prep GG95 (1)-GG102 (3)</p>
+
−
<p>- Dilute Primers</p>
+
−
<p>- Test Fluorescence of GG95-102_1-3 & GG105-108_1-4</p>
+
−
<p>- Purification of Plasmid DNA GG95-102_1-3 & GG105-108_1-4</p>
+
  
 +
<h3>Week of October 10th</h3>
 +
<h5>- Golden Gate GG113-114</h5>
 +
<h5>- Transformation GG103 & 104, GG105-112</h5>
 +
<h5>- LB Chloramphenicol plates made</h5>
 +
<h5>- PCR Purification of UC13a-UC15a</h5>
 +
<h5>- Transformation done GG113-114</h5>
 +
<h5>- LB Broth Made</h5>
 +
<h5>- Mini Prep GG95 (1)-GG102 (3)</h5>
 +
<h5>- Dilute Primers</h5>
 +
<h5>- Test Fluorescence of GG95-102_1-3 & GG105-108_1-4 (by TX-TL)</h5>
 +
<h5>- Purification of Plasmid DNA GG95-102_1-3 & GG105-108_1-4</h5>
  
 +
<h3>Week of October 17th</h3>
 +
<h5>- WIKI WORK!!!</h5>
 
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Latest revision as of 03:49, 20 October 2016

Alverno iGEM 2016

Notebook

Week of June 6th (Limited amount of notes taken)

- Lots of Designing Parts and Primers
- Wiki Work

Week of June 13th:

- Cleaning Restriction Digest and Ligation Done
- Ampicillin plates made
- Ampicillin stock made
- Transform RFP plasmid
- Design Parts and Primers
- Wiki Work

Week of June 20th

- Transformation Done (probably GFP)
- RFP and GRP colonies
- PCR for RFP Coding Gene
- Design Parts and Primers
- Wiki Work

Week of June 27th

- PCR Done for promoters, terminators, some UC parts
- Primer Dilutions Done
- Gel Run with PCR product from Week of June 20th (RFP)
- Miniprep kit for RFP Bba
- TBE Buffer 10x and 1x made
- PCR Purification done on Promoters and Terminators
- Wiki Work

Week of July 4th

- PCR of Vectors, some UC parts
- Primer Dilutions
- Gel Run UC16-18 and UC2 (UC18 ran twice)
- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)
- Plate Reader
- Wiki Work

Week of July 11th

iGEM
- Learn how to use Benchling
- Primers Diluted
iGEM
- Parts Diluted
- PCR done on P1ab, P2ab, T3ab, T4ab
- Gel Run on P1ab, P2ab, T3ab, T4ab
- PCR Clean up done on P1ab, P2ab, T3ab, T4ab
- Chloramphenicol plates made
- Wiki Work

Week of July 18th

- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)
iGEM
- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d
iGEM
- Transformation Done
- Chloramphenicol plates made
- Parts dilutions Done
- PCR of GG Assemblies
- PCR of UC3a-UC9a
- Wiki Work

Week of July 25th

iGEM
- Set up Plate reader
- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts
- PCR Purification of UC3-9, V1a & V2a
iGEM
- Picked Colonies
- Transformation Protocol for GG1-8
- Gel made & Gel made w/ SYBR safe
- PCR of GG Assemblies
- PCR for V1a and V2a, UC7-9
- Dilutions Done
- Gel Run for UC7-9
- Golden Gate Assembly GG9-16
- Wiki Work

Week of August 1st

- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23
- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12
- PCR Purification for UC7-8
- Parts Dilutions done UC2 & UC3-6 & UC7-8
- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23
iGEM
- Fixed Thermocycler
- Gels Made
- Agar Plates made w/ Kan
- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26
iGEM
- Transformation with GG12
- Ludox Calibration
- Test Fluorescence of Plasmids
- Wiki Work

Week of August 8th

iGEM
- Cell Measurement
iGEM
- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR
- PCR Check for GG24-26 & GG27-28
- Made Gel
- Transformation with GG24-26 & GG27-28
- Golden Gate Assembly GG27-28
- Colony PCR Check of GG24-26
- Primer Dilutions
- Plasmid DNA Purification
- LB Kan Broth
- Wiki Work

Week of August 15th

iGEM
- Lab Meet-up with UCL/LC/CV
- Colony PCR Check for GG libraries 27-28
- Gels Made
- Gel Run for GG Library 28
- Grow Cultures GG27
iGEM
- Learn Python
- Check Fluorescence for GG28 & GG27
- PCR UC14a
- Purification of Plasmid DNA
- Wiki Work

Week of August 22nd

iGEM
- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36
- Gels Made
- PCR UC13a and UC15a (Done twice) & UC1
- PCR Purification of UC14 & UC13/15 & UC1
- Golden Gate Assembly for GG29-36
- PCR Check for GG29-36
- LB Kan Broth Made
- Dilution of UC1
- Control run in Plate Reader
iGEM
- Wiki Work

Week of August 29th

iGEM
- Make Gels
- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a
- Golden Gate Assembly GG37-52 & GG53-60
- PCR Check GG37-52 & GG53-60
- PCR Check Gel run for GG37-52
- PCR CDS1a & CDS2a
- PCR Purification of CDS1a & CDS2a
iGEM
- Talk with iGEM Peshawar
- LB Kan Plates Made
- Wiki Work

Week of September 5th

iGEM
- iGEM Team Meeting
- PCR Purification of CDS1a & CDS2a and UC1
- Transformation was done with GG53-60
- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64
- Gels made
- Dilution of CDS1a & CDS2a
- Golden Gate Assembly GG61-64
- PCR of UC1
- LB Kan Plates Made
- PCR Check for GG61-64
iGEM
- TBE 10x Buffer
- Wiki Work

Week of September 12th

iGEM
- Talked with UCL iGEM Team
- LV-LC-CV meet-up
- Google Hangouts with Lambert Georgia iGEM team
iGEM
- Dilution of UC1a
- Transformation of GG29-36
- Golden Gate Assembly GG65-72
- PCR GG61-64
- PCR Check GG61-64
- TX-TL of GFP (+ control)
iGEM
- Outreach workshop with Middle school
- Wiki Work

Week of September 19th

- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE
iGEM
- Run Gel for GG61-64 (done twice) and GG65-72
- LB Kan Plates Made
- Gels Made
- TX-TL GFP Re-run
- Gel Run for UC1 and GG61-64
- Control Test ran using only the ladder for Gels
- TX-TL W & M plasmids
- PCR U3a
- Dilution of Primers
iGEM
- Wiki Work

Week of September 26th

- PCR Purification of V3a
- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94
- PCR Check GG73-74 and GG75-82
- Dilution V3a
- Run Gel V3a
- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74
- TX-TL re-run for W & M plasmids
- Colony PCR GG61-64 and GG65-72
iGEM
- LB Chloramphenicol plates & LB Kanamycin plates
iGEM
- Wiki Work

Week of October 3rd

iGEM
- Middle School Visit
- LB Broth made
- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab
- Dilution V3a
- PCR Check GG83-90
- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab
- TX-TL re-run for W & M plasmids w/ Replicates
- Gels Made
- Mini prep GG28 1-5
- Make TBE 1x
iGEM
- Gel Run P1a-P4a, T1a-T4a, P1ab, P2ab, T3ab, T4ab & GG95-102
- Primers Diluted
- Golden Gate GG103 & GG104 & GG85-102 & GG105-112
- Transformation GG95-102 (done twice)
- Testing Fluorescence on GG28 1-5 w/ Plate Reader
- Wiki Work

Week of October 10th

- Golden Gate GG113-114
- Transformation GG103 & 104, GG105-112
- LB Chloramphenicol plates made
- PCR Purification of UC13a-UC15a
- Transformation done GG113-114
- LB Broth Made
- Mini Prep GG95 (1)-GG102 (3)
- Dilute Primers
- Test Fluorescence of GG95-102_1-3 & GG105-108_1-4 (by TX-TL)
- Purification of Plasmid DNA GG95-102_1-3 & GG105-108_1-4

Week of October 17th

- WIKI WORK!!!