Difference between revisions of "Team:CU-Boulder/Composite Part"

 
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                 <img src="https://static.igem.org/mediawiki/2016/a/a0/T--CU-Boulder--banner3.png" class="img-responsive center-block transp nuoli">
 
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<h3> Composite Part </h3>
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<p class = "main"> Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.</p>
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<h3> Composite Parts </h3>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
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    <img src="https://static.igem.org/mediawiki/2016/c/c1/TCU-Boulder_Comp1.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px">
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  <div class="desc">Mutant EutS with no available AzoPhe</div>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<div class="img2">
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    <img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px">
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  <div class="desc"> Mutant EutS with .1% AzoPhe</div>
 
</div>
 
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<center><div class="img">
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    <img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px">
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  <div class="desc"> 1C3 Plasmid map for Mutant EutS</div>
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<h4>Note</h4>
 
<h4>Note</h4>
 
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
 
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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Latest revision as of 23:13, 3 November 2016

Composite Part

Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.

Mutant EutS with no available AzoPhe
Mutant EutS with .1% AzoPhe
1C3 Plasmid map for Mutant EutS