Difference between revisions of "Team:CU-Boulder/Composite Part"

 
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{{CU-Boulder}}
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{{CU-Boulder/CSS}}
  
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<html lang="en">
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<div class= "row splashala">
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        <div class ="col-md-12 transp scroller">
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<div class="column full_size" >
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                <img src="https://static.igem.org/mediawiki/2016/a/a0/T--CU-Boulder--banner3.png" class="img-responsive center-block transp nuoli">
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            </a>
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        </div>
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    </div>
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</html>
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{{CU-Boulder/Navbar}}
 
<html>
 
<html>
  
  
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<head>
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<style>
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h2 {
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    text-align: center;
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}
  
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h3 {
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    text-align: center;
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}
  
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p.date {
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    text-align: right;
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}
  
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p.mainR{
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    margin-top: 10px;
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    margin-bottom: 10px;
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    margin-right: 80px;
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    margin-left: 480px;
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}
  
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p.mainL{
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    margin-top: 10px;
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    margin-bottom: 10px;
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    margin-right: 450px;
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    margin-left: 80px;
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}
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p.main{
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    margin-top: 10px;
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    margin-bottom: 10px;
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    margin-right: 80px;
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    margin-left: 80px;
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}
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figure{
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  padding: 10px 10px 10px;
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}
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figcaption {
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    caption-side: bottom;
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    text-align: left;
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    font-size:15px
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}
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</style>
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</head>
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<h3> Composite Part </h3>
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<p class = "main"> Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.</p>
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<head>
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<style>
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div.img {
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    margin: 5px;
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    border: 2px solid #ccc;
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    float: left;
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    width: 700px;
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  margin-left: 530px;
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}
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div.img2 {
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    margin: 5px;
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    border: 2px solid #ccc;
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    float: left;
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    width: 600px;
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    margin-left: 190px;
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}
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div.img:hover {
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    border: 2px solid #777;
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}
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div.img img {
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    width: 95%;
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    height: auto;
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}
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div.desc {
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    padding: 15px;
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    text-align: center;
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}
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</style>
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</head>
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<body>
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<div class="img2">
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    <img src="https://static.igem.org/mediawiki/2016/c/c1/TCU-Boulder_Comp1.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px">
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  </a>
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  <div class="desc">Mutant EutS with no available AzoPhe</div>
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</div>
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<div class="img2">
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    <img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px">
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  </a>
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  <div class="desc"> Mutant EutS with .1% AzoPhe</div>
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</div>
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<center><div class="img">
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    <img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px">
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  </a>
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  <div class="desc"> 1C3 Plasmid map for Mutant EutS</div>
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</div>
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</center>
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</body>
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Latest revision as of 23:13, 3 November 2016

Composite Part

Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.

Mutant EutS with no available AzoPhe
Mutant EutS with .1% AzoPhe
1C3 Plasmid map for Mutant EutS