Difference between revisions of "Team:CU-Boulder/Composite Part"
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| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/a/a0/T--CU-Boulder--banner3.png" class="img-responsive center-block transp nuoli"> |
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<h3> Composite Part </h3> | <h3> Composite Part </h3> | ||
| − | <p class = "main"> | + | <p class = "main"> Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.</p> |
| + | <head> | ||
| + | <style> | ||
| + | div.img { | ||
| + | margin: 5px; | ||
| + | border: 2px solid #ccc; | ||
| + | float: left; | ||
| + | width: 700px; | ||
| + | margin-left: 530px; | ||
| + | |||
| + | } | ||
| + | |||
| + | div.img2 { | ||
| + | margin: 5px; | ||
| + | border: 2px solid #ccc; | ||
| + | float: left; | ||
| + | width: 600px; | ||
| + | margin-left: 190px; | ||
| + | } | ||
| + | |||
| + | div.img:hover { | ||
| + | border: 2px solid #777; | ||
| + | } | ||
| + | |||
| + | div.img img { | ||
| + | width: 95%; | ||
| + | height: auto; | ||
| + | } | ||
| + | |||
| + | div.desc { | ||
| + | padding: 15px; | ||
| + | text-align: center; | ||
| + | } | ||
| + | </style> | ||
| + | </head> | ||
| + | <body> | ||
| + | |||
| + | <div class="img2"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/c/c1/TCU-Boulder_Comp1.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px"> | ||
| + | </a> | ||
| + | <div class="desc">Mutant EutS with no available AzoPhe</div> | ||
| + | </div> | ||
| + | |||
| + | <div class="img2"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px"> | ||
| + | </a> | ||
| + | <div class="desc"> Mutant EutS with .1% AzoPhe</div> | ||
| + | </div> | ||
| + | |||
| + | <center><div class="img"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px"> | ||
| + | </a> | ||
| + | <div class="desc"> 1C3 Plasmid map for Mutant EutS</div> | ||
| + | </div> | ||
| + | </center> | ||
| + | </body> | ||
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Latest revision as of 23:13, 3 November 2016
Composite Part
Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.
Mutant EutS with no available AzoPhe
Mutant EutS with .1% AzoPhe
1C3 Plasmid map for Mutant EutS
