Difference between revisions of "Team:CU-Boulder/Composite Part"
| (11 intermediate revisions by 2 users not shown) | |||
| Line 6: | Line 6: | ||
<div class ="col-md-12 transp scroller"> | <div class ="col-md-12 transp scroller"> | ||
<div class="column full_size" > | <div class="column full_size" > | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/a/a0/T--CU-Boulder--banner3.png" class="img-responsive center-block transp nuoli"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 35: | Line 35: | ||
margin-bottom: 10px; | margin-bottom: 10px; | ||
margin-right: 80px; | margin-right: 80px; | ||
| − | margin-left: | + | margin-left: 480px; |
} | } | ||
| Line 66: | Line 66: | ||
<h3> Composite Part </h3> | <h3> Composite Part </h3> | ||
| − | <p class = "main"> | + | <p class = "main"> Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.</p> |
<head> | <head> | ||
<style> | <style> | ||
| Line 74: | Line 74: | ||
float: left; | float: left; | ||
width: 700px; | width: 700px; | ||
| − | + | margin-left: 530px; | |
| + | |||
} | } | ||
| Line 105: | Line 106: | ||
<img src="https://static.igem.org/mediawiki/2016/c/c1/TCU-Boulder_Comp1.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px"> | <img src="https://static.igem.org/mediawiki/2016/c/c1/TCU-Boulder_Comp1.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px"> | ||
</a> | </a> | ||
| − | <div class="desc">Mutant | + | <div class="desc">Mutant EutS with no available AzoPhe</div> |
</div> | </div> | ||
| Line 111: | Line 112: | ||
<img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px"> | <img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px"> | ||
</a> | </a> | ||
| − | <div class="desc"> Mutant | + | <div class="desc"> Mutant EutS with .1% AzoPhe</div> |
</div> | </div> | ||
| − | <div class="img | + | <center><div class="img"> |
<img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px"> | <img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px"> | ||
</a> | </a> | ||
| − | <div class="desc"> 1C3 Plasmid map for | + | <div class="desc"> 1C3 Plasmid map for Mutant EutS</div> |
</div> | </div> | ||
| − | + | </center> | |
</body> | </body> | ||
Latest revision as of 23:13, 3 November 2016
Composite Part
Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.
Mutant EutS with no available AzoPhe
Mutant EutS with .1% AzoPhe
1C3 Plasmid map for Mutant EutS
