Difference between revisions of "Team:CU-Boulder/Composite Part"

Latest revision as of 23:13, 3 November 2016

Composite Part

Mutant Amber-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon placed mid sequence. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.

Mutant EutS with no available AzoPhe

Mutant EutS with .1% AzoPhe

1C3 Plasmid map for Mutant EutS

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      <img src="https://static.igem.org/mediawiki/2016/c/c1/TCU-Boulder_Comp1.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px">
    </a>
-   <div class="desc">Mutant G26-Amber EutS with no available AzoPhe</div>
+   <div class="desc">Mutant EutS with no available AzoPhe</div>
 </div>
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      <img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="560" height="560" style = "margin-left: 15px; margin-top: 15px">
    </a>
-   <div class="desc"> Mutant G26-Amber EutS with .1% AzoPhe</div>
+   <div class="desc"> Mutant EutS with .1% AzoPhe</div>
 </div>
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      <img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px">
    </a>
-   <div class="desc"> 1C3 Plasmid map for G26-Amber EutS</div>
+   <div class="desc"> 1C3 Plasmid map for Mutant EutS</div>
 </div>
 </center>