Difference between revisions of "Team:Northeastern/Basic Part"

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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">basic part special prize</a>. </p>
 
  
  
  
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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                <h3>basic parts</h3>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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                <h4>NADH Oxidase</h4>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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                    <p>This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:</p>
  
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                    <p style="text-align:center;">4 NADH + O<sub>2</sub> → 4 NAD<sup>+</sup> + 2 H<sub>2</sub>O + 4 e<sup>-</sup></p>
  
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                    <p>We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and a modified RBS sequence based on the <a href="http://parts.igem.org/Part:BBa_K1123010"> BBa_K1123010 RBS </a> used by TU Eindhoven 2013 under the FNR promoter. Our version has been optimized to reduce homodimer formation during PCR (<a href="http://parts.igem.org/Part:BBa_K2182001">BBa_K2182004</a>).</p>
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                        <img src="https://static.igem.org/mediawiki/2016/d/d1/T--Northeastern--parts_nox_01.PNG">
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                        <a href="/Team:Northeastern/Parts">Summary</a>
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                        <a href="/Team:Northeastern/Composite_Part">Composite</a>
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                        <a href="/Team:Northeastern/Part_Collection">Collection</a>
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<h4>Note</h4>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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            <a href="http://www.northeastern.edu/">Northeastern University, Boston MA</a> &middot;
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            <a href="https://github.com/omwan/iGEM-wiki-neu16">Github</a>
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Latest revision as of 22:39, 6 November 2016

basic parts

NADH Oxidase

This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:

4 NADH + O2 → 4 NAD+ + 2 H2O + 4 e-

We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and a modified RBS sequence based on the BBa_K1123010 RBS used by TU Eindhoven 2013 under the FNR promoter. Our version has been optimized to reduce homodimer formation during PCR (BBa_K2182004).