Difference between revisions of "Team:Northeastern/Basic Part"

m
 
(2 intermediate revisions by 2 users not shown)
Line 42: Line 42:
 
                     <p style="text-align:center;">4 NADH + O<sub>2</sub> → 4 NAD<sup>+</sup> + 2 H<sub>2</sub>O + 4 e<sup>-</sup></p>
 
                     <p style="text-align:center;">4 NADH + O<sub>2</sub> → 4 NAD<sup>+</sup> + 2 H<sub>2</sub>O + 4 e<sup>-</sup></p>
  
−
                     <p>We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and modified RBS sequences based on the one submitted with the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1155008">fnr coding sequence by Paris Saclay 2013</a> optimized to reduce homodimer formation during PCR (<a href="http://parts.igem.org/Part:BBa_K2182001">BBa_K2182004</a> and <a  href="/">BBa_K2182005</a>).</p>
+
                     <p>We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and a modified RBS sequence based on the <a href="http://parts.igem.org/Part:BBa_K1123010"> BBa_K1123010 RBS </a> used by TU Eindhoven 2013 under the FNR promoter. Our version has been optimized to reduce homodimer formation during PCR (<a href="http://parts.igem.org/Part:BBa_K2182001">BBa_K2182004</a>).</p>
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
Line 58: Line 58:
 
                 <div class="content">
 
                 <div class="content">
 
                     <div id="aboutbuttons">
 
                     <div id="aboutbuttons">
−
                         <a href="/">Summary</a>
+
                         <a href="/Team:Northeastern/Parts">Summary</a>
−
                         <a href="/">Composite</a>
+
                         <a href="/Team:Northeastern/Composite_Part">Composite</a>
−
                         <a href="/">Collection</a>
+
                         <a href="/Team:Northeastern/Part_Collection">Collection</a>
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>

Latest revision as of 22:39, 6 November 2016

basic parts

NADH Oxidase

This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:

4 NADH + O2 → 4 NAD+ + 2 H2O + 4 e-

We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and a modified RBS sequence based on the BBa_K1123010 RBS used by TU Eindhoven 2013 under the FNR promoter. Our version has been optimized to reduce homodimer formation during PCR (BBa_K2182004).