Difference between revisions of "Team:OUC-China"

Latest revision as of 03:19, 11 November 2016

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ABSTRACT

Many applications of synthetic biology require the balanced expression of multiple genes. Our team aimed to develop a tightly regulated element to tune the expression of multiple genes within a polycistron. Inspired by the cellulosome of Clostridium cellulolyticum, we concentrate on stem-loops inserted into the intergentic regions. When transcribed as one polycistron , digested and seperated into several independent fragments, cistrons with 3' end stem-loops varing folding free energy will get different stability. Thus it influences the amount of expressed proteins to realize cistrons concerto. As creative regulating method it is, we have measured and standardized a series of native and designed stem-loops, transforming into a toolkit for a broad use. Our modeling and software is the further exploration for this amazing mean.

Design

Safety

Collaborations

Human Practices

Team

Model

Cistrons Concerto

Thanks:

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.GenScript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM logo-one logo-two

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-								<li><a href="https://2016.igem.org/Team:OUC-China/Project">Introduction</a></li>
+								<li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li>
 								<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
 								<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
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-				<p>As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, we can decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p>
+				<p>Many applications of synthetic biology require the balanced expression of multiple genes. Our team aimed to develop a tightly regulated element to tune the expression of multiple genes within a polycistron. Inspired by the cellulosome of <i>Clostridium  cellulolyticum</i>, we concentrate on stem-loops inserted into the intergentic regions. When transcribed as one polycistron , digested and seperated into several independent fragments, cistrons with 3' end stem-loops varing folding free energy will get different stability. Thus it influences the amount of expressed proteins to realize cistrons concerto. As creative regulating method it is, we have measured and standardized a series of native and designed stem-loops, transforming into a toolkit for a broad use. Our modeling and software is the further exploration for this amazing mean.</p>
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-			<img src="https://static.igem.org/mediawiki/2016/9/99/T--OUC-China--movie-1.fw.png" alt="movie-background">
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-				<a href="#"><img src="https://static.igem.org/mediawiki/2016/4/41/T--OUC-China--menu-icon-1.fw.png" class="img-responsive" alt="Purpose"><p class="text-center">Purpose</p></a>
+				<a href="https://2016.igem.org/Team:OUC-China/Design"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--OUC-China--menu-icon-2.fw.png" class="img-responsive" alt="Design"><p class="text-center" style="margin-top:20px;">Design</p></a>
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-				<a href="#"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--OUC-China--menu-icon-2.fw.png" class="img-responsive" alt="Principle"><p class="text-center">Principle</p></a>
+				<a href="https://2016.igem.org/Team:OUC-China/Model"><img src="https://static.igem.org/mediawiki/2016/4/41/T--OUC-China--menu-icon-1.fw.png" class="img-responsive" alt="Safety"><p class="text-center" style="margin-top:20px;">Safety</p></a>
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-				<a href="#"><img src="https://static.igem.org/mediawiki/2016/0/07/T--OUC-China--menu-icon-3.fw.png" class="img-responsive" alt="Potential"><p class="text-center">Potential</p></a>
+				<a href="https://2016.igem.org/Team:OUC-China/Collaborations"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--OUC-China--menu-icon-4.fw.png" class="img-responsive" alt="Collaborations"><p class="text-center" style="margin-top:20px;">Collaborations</p></a>
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-				<a href="https://2016.igem.org/Team:OUC-China/Attributions"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--OUC-China--menu-icon-4.fw.png" class="img-responsive" alt="Attrbution"><p class="text-center">Attrbutions</p></a>
+				<a href="https://2016.igem.org/Team:OUC-China/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/0/04/T--OUC-China--menu-icon-6.fw.png" class="img-responsive" alt="Human Practices"><p class="text-center" style="margin-top:20px;">Human Practices</p></a>
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+				<a href="https://2016.igem.org/Team:OUC-China/Team"><img src="https://static.igem.org/mediawiki/2016/a/ad/T--OUC-China--menu-icon-5.fw.png" class="img-responsive" alt="Team"><p class="text-center" style="margin-top:20px;">Team</p></a>
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-				<a href="https://2016.igem.org/Team:OUC-China/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/0/04/T--OUC-China--menu-icon-6.fw.png" class="img-responsive" alt="Human Practices"><p class="text-center">Human Practices</p></a>
+				<a href="https://2016.igem.org/Team:OUC-China/Software"><img src="https://static.igem.org/mediawiki/2016/0/07/T--OUC-China--menu-icon-3.fw.png" class="img-responsive" alt="Model"><p class="text-center" style="margin-top:20px;">Model</p></a>
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 				<h3>Thanks:</h3>
+				<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
-				<p><b>1.</b>Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
+				<p>2.NEW ENGLAND Biolabs</p>
-				<p><b>2.</b>NEW ENGLAND Biolabs</p>
+				<p>3.GenScript</p>
 			</div>
 			<div class="col-md-5 Contact">
 				<h3>Contact us:</h3>
 				<p><b>E-mail</b>: oucigem@163.com</p>
 				<p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p>