Difference between revisions of "Team:Uppsala"

 
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          <h3> Project overview <small>What we are aiming for</small> </h3>
 
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<img class="img-responsive img-rounded" style="clip: rect(0px,500px,0px,0px); float:none; margin:auto;" src="https://static.igem.org/mediawiki/2016/2/25/CHIPGINEERING.png" />
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We are developing a method to make CRISPR and microfluidics more available to iGEM teams and researchers. The technique will be used to fuse a fluorescent protein called UnaG to a genomic protein in both prokaryotes and eukaryotes. We are including state of the art research involving the CRISPR associated protein CPF1 and microfluidic methods. </p>
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<p> UnaG is a fluorescent protein that needs bilirubin as a co-factor in order to fluoresce. Bilirubin binds non-covalently, which facilitates the possibility of creating inducible fluorescent switches from UnaG. Bilirubin occurs naturally in higher vertebrate cells, making it suitable as a biosensor for research on vertebrates. It is therefore convenient to use UnaG together with CRISPR systems, such as CRISPR/CPF1. </p>
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<p> CPF1 cuts downstream of the PAM sites and leaves 5’ overhangs. By providing UnaG with the complementary overhangs, we could insert this fluorescent protein in the genome of our host. In order to increase the chances of correct insertion, we aim to engineer UnaG with homology arms, which enables cells to insert UnaG by means of homologous recombination in the exact position where the genome has been cut. </p>
 
  
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<p> To facilitate the insertion of the genomic material we will design a microfluidic chip capable
 
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of transformation. This will be done through soft lithography by 3D printing a mold and
 
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baking a PDMS chip on it. A microfluidic chip will reduce the amount of reagents needed to perform a transformation, which could potentially reduce the cost and workload of a conventional transformation. The chip methods are not size-dependent, therefore it will be possible to do any given plasmid insertion with the same device. </p>
 
  
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<p> By using this chip, cell transformation becomes simpler and cheaper to do for other iGEM teams and small laboratories. In our project we will use it along with CRISPR to fuse UnaG with a genomic protein in yeast, but a microfluidic chip could potentially be used for any transformation technique. </p>
 
  
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                                <video width="920" height="680" controls poster="https://static.igem.org/mediawiki/2016/5/59/T--Uppsala--startpage_chip_closeup.png">
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                                    <source src="https://static.igem.org/mediawiki/2016/a/a3/T--Uppsala--Movie_Trailer.mp4" type="video/mp4" > Your browser does not support the video tag.
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<p>Here is a trailer for our guide to making microfluidic chips in a simple way! Check out the microfluidics page, or click the corresponding button below, for the full movie and an in depth manual on how to make your own chips!
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</p>
 
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<a href="https://2016.igem.org/Team:Uppsala/Project/Microfluidics">
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                            <div class="panel-body"> <h3 style="color: ghostwhite;">See more over at the microfluidics page </h3> </div>
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                         <h4> CRISPR/CPF1 </h4>
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                         <h4 style="color: ghostwhite;"> Design </h4>
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                        <h4 style="color: ghostwhite;"> Modeling </h4>
 
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                         <h4> UnaG </h4>
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                        <h4 style="color: ghostwhite;"> Project description </h4>
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                         <h4 style="color: ghostwhite;"> Instruction movie </h4>
 
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                         <h3> Microfluidics </h3>
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                         <h4 style="color: ghostwhite;"> The manual </h4>
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                        <h4 style="color: ghostwhite;"> Results </h4>
 
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<a  href="https://2016.igem.org/Team:Uppsala/Project/CRISPR">
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                            <div class="panel-body"> <h4 style="color: ghostwhite;">Read about our work with CRISPR & CPF1 </h4> </div>
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<a  href="https://2016.igem.org/Team:Uppsala/Project/UnaG">
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                            <div class="panel-body"> <h4 style="color: ghostwhite;">Bask in the glow of UnaG </h4> </div>
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Latest revision as of 07:06, 11 November 2016

Playing with Bootstrap

Here is a trailer for our guide to making microfluidic chips in a simple way! Check out the microfluidics page, or click the corresponding button below, for the full movie and an in depth manual on how to make your own chips!

See more over at the microfluidics page

Design

Modeling

Project description

Instruction movie

The manual

Results

Read about our work with CRISPR & CPF1

Bask in the glow of UnaG