Difference between revisions of "Team:OUC-China/Parts"

 
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        <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/0/0d/T--OUC-China--Part_Banner.jpg" class="img-responsive" alt="parts-banner"></div>
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<h1>Parts</h1>
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<h3 class="text-center">Parts list</h3>
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<br>
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<p style="width:80%;margin:0 auto;" >This year, we devoted ourselves to the exploitation of new regulatory parts and the exploration of a kind of novel regulatory method. As a results, we constructed a series of stem loops with different folding free energy as a basic regulatory part, also a series of composite parts of fluorescent reporter system to test the the regulatory effect of various stem-loops.<br>Here is the summary of the parts that we constructed, some of which we have submitted to the registry.Characterization of an existing BioBrick Part or Device(<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>)</p>
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<div class="col-md-4 text-center">
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<br>
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<a href="http://parts.igem.org/Part:BBa_K2047001">BBa_K2047001</a><br />
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<div class="table-responsive" style="width:80%;margin:0 auto;" >
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<a href="http://parts.igem.org/Part:BBa_K2047002">BBa_K2047002</a><br />
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<table class="table table-bordered">
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<a href="http://parts.igem.org/Part:BBa_K2047003">BBa_K2047003</a><br />
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  <tr>
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<a href="http://parts.igem.org/Part:BBa_K2047004">BBa_K2047004</a><br />
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<td>Part Number</td>
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<a href="http://parts.igem.org/Part:BBa_K2047005">BBa_K2047005</a><br />
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<td>Type</td>
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<a href="http://parts.igem.org/Part:BBa_K2047006">BBa_K2047006</a><br />
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<td>Description</td>
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<a href="http://parts.igem.org/Part:BBa_K2047007">BBa_K2047007</a><br />
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<td>Designer</td>
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<a href="http://parts.igem.org/Part:BBa_K2047008">BBa_K2047008</a><br />
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<td>Length(bp)</td>
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</div>
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  </tr>
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<div class="col-md-4 text-center">
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  <tr>
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<a href="http://parts.igem.org/Part:BBa_K2047101">BBa_K2047101</a><br />
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<td><a href="http://parts.igem.org/Part:BBa_K2047001">BBa_K2047001</a></td>
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<a href="http://parts.igem.org/Part:BBa_K2047102">BBa_K2047102</a><br />
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<td>Composite</td>
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<a href="http://parts.igem.org/Part:BBa_K2047103">BBa_K2047103</a><br />
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<td>GFP_Stem-loop with free energy of -25.6 measured by Mfold</td>
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<a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a><br />
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<td>Yu Jiang</td>
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<a href="http://parts.igem.org/Part:BBa_K2047105">BBa_K2047105</a><br />
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<td>801</td>
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<a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a><br />
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  </tr>
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<a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a><br />
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  <tr>
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<a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a><br />
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<td><a href="http://parts.igem.org/Part:BBa_K2047002">BBa_K2047002</a></td>
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</div>
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<td>Basic</td>
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<div class="col-md-4 text-center">
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<td>Stem-loop with free energy of -14.9 measured by Mfold</td>
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<a href="http://parts.igem.org/Part:BBa_K2047111">BBa_K2047111</a><br />
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<td>Yu Jiang</td>
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<a href="http://parts.igem.org/Part:BBa_K2047222">BBa_K2047222</a><br />
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<td>80</td>
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<a href="http://parts.igem.org/Part:BBa_K2047333">BBa_K2047333</a><br />
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  </tr>
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<a href="http://parts.igem.org/Part:BBa_K2047666">BBa_K2047666</a><br />
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  <tr>
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</div>
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<td><a href="http://parts.igem.org/Part:BBa_K2047003">BBa_K2047003</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -38.7measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>809</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047004">BBa_K2047004</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -38.7measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>89</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047005">BBa_K2047005</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -30.1 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>94</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047006">BBa_K2047006</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -38.5 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>102</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047007">BBa_K2047007</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -34.4 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>98</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047008">BBa_K2047008</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -38.8 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>104</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047009">BBa_K2047009</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -25.6 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>81</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047010">BBa_K2047010</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -38.8 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>101</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047011">BBa_K2047011</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -51.4 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>107</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047013">BBa_K2047013</a></td>
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<td>Basic</td>
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<td>Stem-loop with free energy of -44.9 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>78</td>
 +
  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047101">BBa_K2047101</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -30.1 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>814</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047102">BBa_K2047102</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -14.9 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>800</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047103">BBa_K2047103</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>821</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a></td>
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<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -34.4 measured by Mfold</td>
 +
<td>Yu Jiang</td>
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<td>818</td>
 +
  </tr>
 +
  <tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2047105">BBa_K2047105</a></td>
 +
<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -51.4measured by Mfold</td>
 +
<td>Yu Jiang</td>
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<td>827</td>
 +
  </tr>
 +
  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a></td>
 +
<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -38.5 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>822</td>
 +
  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a></td>
 +
<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -44.9 measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>798</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td>
 +
<td>Yu Jiang</td>
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<td>824</td>
 +
  </tr>
 +
  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047111">BBa_K2047111</a></td>
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<td>Composite</td>
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<td>K206000+I13504</td>
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<td>Yu Jiang</td>
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<td>1013</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047222">BBa_K2047222</a></td>
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<td>Composite</td>
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<td>I13453+I13504</td>
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<td>Yu Jiang</td>
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<td>1013</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047333">BBa_K2047333</a></td>
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<td>Composite</td>
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<td>K206001+I13504</td>
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<td>Yu Jiang</td>
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<td>1013</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047666">BBa_K2047666</a></td>
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<td>Basic</td>
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<td>RNase E site</td>
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<td>Yu Jiang</td>
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<td>12</td>
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  </tr>
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</table>
 
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<h3>Thanks:</h3>
 
<h3>Thanks:</h3>
 
<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
 
<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
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<p>2.NEW ENGLAND Biolabs</p>
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<p>2.NEW ENGLAND Biolabs</p>
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<p>3.Genscript</p>
 
</div>
 
</div>
 
<div class="col-md-5 Contact">
 
<div class="col-md-5 Contact">

Latest revision as of 01:05, 12 November 2016

Parts

parts-banner

Parts list


This year, we devoted ourselves to the exploitation of new regulatory parts and the exploration of a kind of novel regulatory method. As a results, we constructed a series of stem loops with different folding free energy as a basic regulatory part, also a series of composite parts of fluorescent reporter system to test the the regulatory effect of various stem-loops.
Here is the summary of the parts that we constructed, some of which we have submitted to the registry.Characterization of an existing BioBrick Part or Device(BBa_I0500)


Part Number Type Description Designer Length(bp)
BBa_K2047001 Composite GFP_Stem-loop with free energy of -25.6 measured by Mfold Yu Jiang 801
BBa_K2047002 Basic Stem-loop with free energy of -14.9 measured by Mfold Yu Jiang 80
BBa_K2047003 Composite GFP_Stem-loop with free energy of -38.7measured by Mfold Yu Jiang 809
BBa_K2047004 Basic Stem-loop with free energy of -38.7measured by Mfold Yu Jiang 89
BBa_K2047005 Basic Stem-loop with free energy of -30.1 measured by Mfold Yu Jiang 94
BBa_K2047006 Basic Stem-loop with free energy of -38.5 measured by Mfold Yu Jiang 102
BBa_K2047007 Basic Stem-loop with free energy of -34.4 measured by Mfold Yu Jiang 98
BBa_K2047008 Basic Stem-loop with free energy of -38.8 measured by Mfold Yu Jiang 104
BBa_K2047009 Basic Stem-loop with free energy of -25.6 measured by Mfold Yu Jiang 81
BBa_K2047010 Basic Stem-loop with free energy of -38.8 measured by Mfold Yu Jiang 101
BBa_K2047011 Basic Stem-loop with free energy of -51.4 measured by Mfold Yu Jiang 107
BBa_K2047013 Basic Stem-loop with free energy of -44.9 measured by Mfold Yu Jiang 78
BBa_K2047101 Composite GFP_Stem-loop with free energy of -30.1 measured by Mfold Yu Jiang 814
BBa_K2047102 Composite GFP_Stem-loop with free energy of -14.9 measured by Mfold Yu Jiang 800
BBa_K2047103 Composite GFP_Stem-loop with free energy of -38.8 measured by Mfold Yu Jiang 821
BBa_K2047104 Composite GFP_Stem-loop with free energy of -34.4 measured by Mfold Yu Jiang 818
BBa_K2047105 Composite GFP_Stem-loop with free energy of -51.4measured by Mfold Yu Jiang 827
BBa_K2047106 Composite GFP_Stem-loop with free energy of -38.5 measured by Mfold Yu Jiang 822
BBa_K2047107 Composite GFP_Stem-loop with free energy of -44.9 measured by Mfold Yu Jiang 798
BBa_K2047108 Composite GFP_Stem-loop with free energy of -38.8 measured by Mfold Yu Jiang 824
BBa_K2047111 Composite K206000+I13504 Yu Jiang 1013
BBa_K2047222 Composite I13453+I13504 Yu Jiang 1013
BBa_K2047333 Composite K206001+I13504 Yu Jiang 1013
BBa_K2047666 Basic RNase E site Yu Jiang 12



Cistrons Concerto

Thanks:

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.Genscript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM logo-one logo-two