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</nav> | </nav> | ||
<div class="clearfix"></div> | <div class="clearfix"></div> | ||
− | + | <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/0/0d/T--OUC-China--Part_Banner.jpg" class="img-responsive" alt="parts-banner"></div> | |
− | + | <h3 class="text-center">Parts list</h3> | |
− | </ | + | <br> |
− | <div class=" | + | <p style="width:80%;margin:0 auto;" >This year, we devoted ourselves to the exploitation of new regulatory parts and the exploration of a kind of novel regulatory method. As a results, we constructed a series of stem loops with different folding free energy as a basic regulatory part, also a series of composite parts of fluorescent reporter system to test the the regulatory effect of various stem-loops.<br>Here is the summary of the parts that we constructed, some of which we have submitted to the registry.Characterization of an existing BioBrick Part or Device(<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>)</p> |
− | < | + | <br> |
− | <a href="http://parts.igem.org/Part:BBa_K2047001">BBa_K2047001</a>< | + | <div class="table-responsive" style="width:80%;margin:0 auto;" > |
− | <a href="http://parts.igem.org/Part:BBa_K2047002">BBa_K2047002</a>< | + | <table class="table table-bordered"> |
− | <a href="http://parts.igem.org/Part:BBa_K2047003">BBa_K2047003</a>< | + | <tr> |
− | <a href="http://parts.igem.org/Part:BBa_K2047004">BBa_K2047004</a>< | + | <td>Part Number</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047005">BBa_K2047005</a>< | + | <td>Type</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047006">BBa_K2047006</a>< | + | <td>Description</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047007">BBa_K2047007</a>< | + | <td>Designer</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047008">BBa_K2047008</a>< | + | <td>Length(bp)</td> |
− | + | </tr> | |
− | + | <tr> | |
− | <a href="http://parts.igem.org/Part:BBa_K2047101">BBa_K2047101</a>< | + | <td><a href="http://parts.igem.org/Part:BBa_K2047001">BBa_K2047001</a></td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047102">BBa_K2047102</a>< | + | <td>Composite</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047103">BBa_K2047103</a>< | + | <td>GFP_Stem-loop with free energy of -25.6 measured by Mfold</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a>< | + | <td>Yu Jiang</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047105">BBa_K2047105</a>< | + | <td>801</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a>< | + | </tr> |
− | <a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a>< | + | <tr> |
− | <a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a>< | + | <td><a href="http://parts.igem.org/Part:BBa_K2047002">BBa_K2047002</a></td> |
− | + | <td>Basic</td> | |
− | + | <td>Stem-loop with free energy of -14.9 measured by Mfold</td> | |
− | <a href="http://parts.igem.org/Part:BBa_K2047111">BBa_K2047111</a>< | + | <td>Yu Jiang</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047222">BBa_K2047222</a>< | + | <td>80</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047333">BBa_K2047333</a>< | + | </tr> |
− | <a href="http://parts.igem.org/Part:BBa_K2047666">BBa_K2047666</a>< | + | <tr> |
− | </ | + | <td><a href="http://parts.igem.org/Part:BBa_K2047003">BBa_K2047003</a></td> |
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -38.7measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>809</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047004">BBa_K2047004</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -38.7measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>89</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047005">BBa_K2047005</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -30.1 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>94</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047006">BBa_K2047006</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -38.5 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>102</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047007">BBa_K2047007</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -34.4 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>98</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047008">BBa_K2047008</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -38.8 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>104</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047009">BBa_K2047009</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -25.6 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>81</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047010">BBa_K2047010</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -38.8 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>101</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047011">BBa_K2047011</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -51.4 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>107</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047013">BBa_K2047013</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>Stem-loop with free energy of -44.9 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>78</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047101">BBa_K2047101</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -30.1 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>814</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047102">BBa_K2047102</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -14.9 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>800</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047103">BBa_K2047103</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>821</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -34.4 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>818</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047105">BBa_K2047105</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -51.4measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>827</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -38.5 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>822</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -44.9 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>798</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>824</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047111">BBa_K2047111</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>K206000+I13504</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>1013</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047222">BBa_K2047222</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>I13453+I13504</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>1013</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047333">BBa_K2047333</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>K206001+I13504</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>1013</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047666">BBa_K2047666</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td>RNase E site</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>12</td> | ||
+ | </tr> | ||
+ | </table> | ||
</div> | </div> | ||
<br /> | <br /> | ||
Line 150: | Line 303: | ||
<h3>Thanks:</h3> | <h3>Thanks:</h3> | ||
<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p> | <p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p> | ||
− | <p>2.NEW ENGLAND Biolabs</p> | + | <p>2.NEW ENGLAND Biolabs</p> |
+ | <p>3.Genscript</p> | ||
</div> | </div> | ||
<div class="col-md-5 Contact"> | <div class="col-md-5 Contact"> |
Latest revision as of 01:05, 12 November 2016
Parts list
This year, we devoted ourselves to the exploitation of new regulatory parts and the exploration of a kind of novel regulatory method. As a results, we constructed a series of stem loops with different folding free energy as a basic regulatory part, also a series of composite parts of fluorescent reporter system to test the the regulatory effect of various stem-loops.
Here is the summary of the parts that we constructed, some of which we have submitted to the registry.Characterization of an existing BioBrick Part or Device(BBa_I0500)
Part Number | Type | Description | Designer | Length(bp) |
BBa_K2047001 | Composite | GFP_Stem-loop with free energy of -25.6 measured by Mfold | Yu Jiang | 801 |
BBa_K2047002 | Basic | Stem-loop with free energy of -14.9 measured by Mfold | Yu Jiang | 80 |
BBa_K2047003 | Composite | GFP_Stem-loop with free energy of -38.7measured by Mfold | Yu Jiang | 809 |
BBa_K2047004 | Basic | Stem-loop with free energy of -38.7measured by Mfold | Yu Jiang | 89 |
BBa_K2047005 | Basic | Stem-loop with free energy of -30.1 measured by Mfold | Yu Jiang | 94 |
BBa_K2047006 | Basic | Stem-loop with free energy of -38.5 measured by Mfold | Yu Jiang | 102 |
BBa_K2047007 | Basic | Stem-loop with free energy of -34.4 measured by Mfold | Yu Jiang | 98 |
BBa_K2047008 | Basic | Stem-loop with free energy of -38.8 measured by Mfold | Yu Jiang | 104 |
BBa_K2047009 | Basic | Stem-loop with free energy of -25.6 measured by Mfold | Yu Jiang | 81 |
BBa_K2047010 | Basic | Stem-loop with free energy of -38.8 measured by Mfold | Yu Jiang | 101 |
BBa_K2047011 | Basic | Stem-loop with free energy of -51.4 measured by Mfold | Yu Jiang | 107 |
BBa_K2047013 | Basic | Stem-loop with free energy of -44.9 measured by Mfold | Yu Jiang | 78 |
BBa_K2047101 | Composite | GFP_Stem-loop with free energy of -30.1 measured by Mfold | Yu Jiang | 814 |
BBa_K2047102 | Composite | GFP_Stem-loop with free energy of -14.9 measured by Mfold | Yu Jiang | 800 |
BBa_K2047103 | Composite | GFP_Stem-loop with free energy of -38.8 measured by Mfold | Yu Jiang | 821 |
BBa_K2047104 | Composite | GFP_Stem-loop with free energy of -34.4 measured by Mfold | Yu Jiang | 818 |
BBa_K2047105 | Composite | GFP_Stem-loop with free energy of -51.4measured by Mfold | Yu Jiang | 827 |
BBa_K2047106 | Composite | GFP_Stem-loop with free energy of -38.5 measured by Mfold | Yu Jiang | 822 |
BBa_K2047107 | Composite | GFP_Stem-loop with free energy of -44.9 measured by Mfold | Yu Jiang | 798 |
BBa_K2047108 | Composite | GFP_Stem-loop with free energy of -38.8 measured by Mfold | Yu Jiang | 824 |
BBa_K2047111 | Composite | K206000+I13504 | Yu Jiang | 1013 |
BBa_K2047222 | Composite | I13453+I13504 | Yu Jiang | 1013 |
BBa_K2047333 | Composite | K206001+I13504 | Yu Jiang | 1013 |
BBa_K2047666 | Basic | RNase E site | Yu Jiang | 12 |
Cistrons Concerto
Thanks:
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
3.Genscript
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM