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<div class="container"> | <div class="container"> | ||
− | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | + | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> |
<div class="container"> | <div class="container"> | ||
<div class="navbar-header"> | <div class="navbar-header"> | ||
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<span class="icon-bar"></span> | <span class="icon-bar"></span> | ||
</button> | </button> | ||
− | <a class="navbar-brand oucBrand" href="#"><img src="https://static.igem.org/mediawiki/2016/f/fc/T--OUC-China--nav-logo-1.fw.png" alt="Brand"></a> | + | <a class="navbar-brand oucBrand" href="#"><img src="https://static.igem.org/mediawiki/2016/f/fc/T--OUC-China--nav-logo-1.fw.png" alt="Brand"></a> |
</div> | </div> | ||
<div class="collapse navbar-collapse" id="example-navbar-collapse"> | <div class="collapse navbar-collapse" id="example-navbar-collapse"> | ||
<ul class="nav navbar-nav" id="oucNav"> | <ul class="nav navbar-nav" id="oucNav"> | ||
<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | ||
− | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a> |
− | + | <ul class="dropdown-menu"> | |
− | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="">Parts<span class="caret"></span></a> | + | <li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li> |
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Parts">Parts</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Parts">Parts</a></li> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Basic_Part">Basic part</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Basic_Part">Basic part</a></li> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Composite_Part">Composite parts</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Composite_Part">Composite parts</a></li> | ||
− | <li class="active"><a href=" | + | <li class="active"><a href="https://2016.igem.org/Team:OUC-China/Part_Collection">Part collection</a></li> |
</ul> | </ul> | ||
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− | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#"> | + | |
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− | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li> |
− | + | <li><a href="https://2016.igem.org/Team:OUC-China/Communicating_&_improving">Communicating & improving</a></li> | |
− | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Investigating_&_promoting">Investigating & promoting</a></li> |
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | ||
</ul> | </ul> | ||
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− | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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</nav> | </nav> | ||
<div class="clearfix"></div> | <div class="clearfix"></div> | ||
− | + | <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/0/0d/T--OUC-China--Part_Banner.jpg" class="img-responsive" alt="parts-banner"></div> | |
− | + | <h3 class="text-center">Part Collection</h3> | |
− | </ | + | <br> |
− | <div class=" | + | <p style="width:80%;margin:0 auto;">We devoted to constructing a library of stem-loops to regulate the expression of protein and exploring a toolkit.<br>We tested both native stem-loops and designed stem-loops and we found some parts can coordinate the expression well. Therefore, we submitted these parts to iGEM parts registry with detailed characterization. Then, with the demands of bigger library and more detailed data, we collected these measured stem-loops with various folding free energy to work on constructing a toolkit. </p> |
− | < | + | <br> |
− | < | + | <div class="table-responsive" style="width:80%;margin:0 auto;" > |
− | <a href="http://parts.igem.org/Part: | + | <table class="table table-bordered"> |
− | < | + | <tr> |
− | <a href="http://parts.igem.org/Part: | + | <td>Part Number</td> |
− | < | + | <td>Type</td> |
− | <a href="http://parts.igem.org/Part: | + | <td>Description</td> |
− | < | + | <td>Designer</td> |
− | <a href="http://parts.igem.org/Part: | + | <td>Length(bp)</td> |
− | + | </tr> | |
− | + | <tr> | |
− | < | + | <td><a href="http://parts.igem.org/Part:BBa_K2047001">BBa_K2047001</a></td> |
− | <a href="http://parts.igem.org/Part: | + | <td>Composite</td> |
− | < | + | <td>GFP_Stem-loop with free energy of -25.6 measured by Mfold</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a>< | + | <td>Yu Jiang</td> |
− | < | + | <td>801</td> |
− | <a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a>< | + | </tr> |
− | <a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a>< | + | <tr> |
− | <a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a>< | + | <td><a href="http://parts.igem.org/Part:BBa_K2047003">BBa_K2047003</a></td> |
− | </ | + | <td>Composite</td> |
+ | <td>GFP_Stem-loop with free energy of -38.7measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>809</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047101">BBa_K2047101</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -30.1 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>814</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047102">BBa_K2047102</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -14.9 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>800</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047103">BBa_K2047103</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>821</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -34.4 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>818</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -38.5 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>822</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -44.9 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>798</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td> | ||
+ | <td>Yu Jiang</td> | ||
+ | <td>824</td> | ||
+ | </tr> | ||
+ | </table> | ||
</div> | </div> | ||
− | |||
<br /> | <br /> | ||
<br /> | <br /> | ||
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<div class="col-md-1"></div> | <div class="col-md-1"></div> | ||
<div class="col-md-5"> | <div class="col-md-5"> | ||
− | <h3> | + | <h3>Thanks</h3> |
− | + | <p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p> | |
− | <p> | + | <p>2.NEW ENGLAND Biolabs</p> |
− | <p> | + | <p>3.Genscript</p> |
− | <p> | + | |
</div> | </div> | ||
− | + | <div class="col-md-5 Contact"> | |
− | <div class="col-md-5"> | + | |
<h3>Contact us:</h3> | <h3>Contact us:</h3> | ||
− | < | + | <p><b>E-mail</b>: oucigem@163.com</p> |
− | + | <p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> | |
− | <p> | + | <p>We are OUC-iGEM |
− | + | <img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" width="70" height="70" alt="logo-one"> | |
− | <p>We are OUC-iGEM<img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" alt="logo-one"><img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" alt="logo-two"></p> | + | <img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" width="70" height="70" alt="logo-two"> |
+ | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <div | + | <div class="oucBottom"> |
− | </div> | + | </div> |
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 01:09, 12 November 2016
Part Collection
We devoted to constructing a library of stem-loops to regulate the expression of protein and exploring a toolkit.
We tested both native stem-loops and designed stem-loops and we found some parts can coordinate the expression well. Therefore, we submitted these parts to iGEM parts registry with detailed characterization. Then, with the demands of bigger library and more detailed data, we collected these measured stem-loops with various folding free energy to work on constructing a toolkit.
Part Number | Type | Description | Designer | Length(bp) |
BBa_K2047001 | Composite | GFP_Stem-loop with free energy of -25.6 measured by Mfold | Yu Jiang | 801 |
BBa_K2047003 | Composite | GFP_Stem-loop with free energy of -38.7measured by Mfold | Yu Jiang | 809 |
BBa_K2047101 | Composite | GFP_Stem-loop with free energy of -30.1 measured by Mfold | Yu Jiang | 814 |
BBa_K2047102 | Composite | GFP_Stem-loop with free energy of -14.9 measured by Mfold | Yu Jiang | 800 |
BBa_K2047103 | Composite | GFP_Stem-loop with free energy of -38.8 measured by Mfold | Yu Jiang | 821 |
BBa_K2047104 | Composite | GFP_Stem-loop with free energy of -34.4 measured by Mfold | Yu Jiang | 818 |
BBa_K2047106 | Composite | GFP_Stem-loop with free energy of -38.5 measured by Mfold | Yu Jiang | 822 |
BBa_K2047107 | Composite | GFP_Stem-loop with free energy of -44.9 measured by Mfold | Yu Jiang | 798 |
BBa_K2047108 | Composite | GFP_Stem-loop with free energy of -38.8 measured by Mfold | Yu Jiang | 824 |
Cistrons Concerto
Thanks
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
3.Genscript
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM