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| − | == | + | {{LMU-TUM_Munich|navClass=materials}} |
| − | + | =Materials= | |
| − | + | ==Enzymes and Proteins== | |
| − | + | ===Enzymes=== | |
| − | + | <div class="white-box"> | |
| − | + | <ul> | |
| − | + | <li>Lysozym (22770 U/mg) (AppliChem GmbH, Darmstadt) | |
| − | + | <li>Pfu-Ultra II DNA Polymerase (Promega GmbH, Mannheim) | |
| − | + | <li>T4-DNA-Ligase (200 CEU/μl) (Fermentas GmbH, St. Leon-Rot) | |
| + | <li>Alkalische Phosphatase aus der arktischen Garnele (1 U/μl)(Thermo Fisher Scientific Inc., St. Leon-Rot) | ||
| + | <li>FastAP thermosensitive alkalische Phosphatase (1 U/μl) (Thermo Fisher Scientific Inc., St. Leon-Rot) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ===Restriction enzymes=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>AfeI / Eco47III (Thermo Fisher Scientific Inc., St. Leon-Rot) | ||
| + | <li>HindIII (Thermo Fisher Scientific Inc., St. Leon-Rot) | ||
| + | <li>DpnI (Thermo Fisher Scientific Inc., St. Leon-Rot) | ||
| + | <li>MlyI (New England Biolabs GmbH, Frankfurt am Main) | ||
| + | <li>NcoI (Fermentas GmbH, St. Leon-Rot) | ||
| + | <li>NheI (Fermentas GmbH, St. Leon-Rot) | ||
| + | <li>SapI (New England Biolabs GmbH, Frankfurt am Main) | ||
| + | <li>StuI / Eco147I (Fermentas GmbH, St. Leon-Rot) | ||
| + | <li>NgoMIV (New England Biolabs GmbH, Frankfurt am Main) | ||
| + | <li>XbaI (Thermo Fisher Scientific Inc., St. Leon-Rot) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ===Proteins=== | ||
| + | <div class="white-box"> | ||
| + | Albumin Fraktion V (BSA) (AppliChem GmbH, Darmstadt) | ||
| + | </div> | ||
| + | |||
| + | ==Chemicals== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>2,2'-Dithiodipyridin (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>2-Mercaptoethanol (AppliChem GmbH, Darmstadt) | ||
| + | <li>Acetonitril (LC-MS grade) (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>30% Acrylamid mit 0,8% Bisacrylamid (Serva Electrophoresis GmbH, Heidelberg) | ||
| + | <li>Agarose (peqlab Biotechnologie GmbH, Erlangen) | ||
| + | <li>Agarose (low melting point) (InvitrogenAG, Basel, Schweiz) | ||
| + | <li>Ameisensäure (LC-MS grade) (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Ammoniak, 25 %ig (Honeywell Deutschland AG, Neunkirchen) | ||
| + | <li>Ammoniumacetat (AppliChem GmbH, Darmstadt) | ||
| + | <li>Ammoniumchloris (NH4Cl) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Ammoniumperoxodisulfat (APS) (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Ampicillin, Natriumsalz (Formedium, Hunstanton, UK) | ||
| + | <li>Anhydrotetrazyklin (aTc) (Acros Oranics, Geel, Belgien) | ||
| + | <li>Antifoam A, 30 %ige Stammlösung (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Bacto Agar Becton (DIFCO Laboratories GmbH, Augsburg) | ||
| + | <li>Bacto Tryptone Becton (BD, DIFCO Laboratories GmbH, Augsburg) | ||
| + | <li>Bacto Yeast Extract Becton (BD, DIFCO Laboratories GmbH, Augsburg) | ||
| + | <li>Betain, Monohydrat (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Borsäure (AppliChem GmbH, Darmstadt) | ||
| + | <li>Bromphenolblau (AppliChem GmbH, Darmstadt) | ||
| + | <li>Coomassie Brilliantblau R-250 (AppliChem GmbH, Darmstadt) | ||
| + | <li>Chloramphenicol (AppliChem GmbH, Darmstadt) | ||
| + | <li>D(+)-Glukose, Monohydrat (AppliChem GmbH, Darmstadt) | ||
| + | <li>D(+)-Saccharose (AppliChem GmbH, Darmstadt | ||
| + | <li>Dimethylformamid (DMF) (Merck KG, Darmstadt) | ||
| + | <li>Dithiodiethanol (Acros Oranics, Geel, Belgien) | ||
| + | <li>dNTPs (InvitrogenAG, Basel, Schweiz) | ||
| + | <li>Essigsäure, 99,9 % (Honeywell Deutschland AG, Neunkirchen) | ||
| + | <li>Ethanol p. a., 99,9 % (Honeywell Deutschland AG, Neunkirchen) | ||
| + | <li>Ethidiumbromid (AppliChem GmbH, Darmstadt) | ||
| + | <li>Ethylendinitrilo-tetraessigsäure-Na2 (EDTA) (AppliChem GmbH, Darmstadt) | ||
| + | <li>L-Glycin (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | <li>Glycerin (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | <li>Guanidiniumchlorid (Nigu Chemie GmbH, Waldkraiburg) | ||
| + | <li>Harnstoff (CH4N2O) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Helipur H plus N (B. Braun Melsungen AG, Melsungen) | ||
| + | <li>Imidazol (Merck KG, Darmstadt) | ||
| + | <li>Isopropanol (Honeywell Deutschland AG, Neunkirchen) | ||
| + | <li>Kaliumchlorid (KCl) (Merck KG, Darmstadt) | ||
| + | <li>Kaliumdihydrogenphosphat (KH2PO4) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Kaliumsulfat (K2SO4) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Magnesiumchlorid (MgCl2 · 6 H2O) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Methanol (Honeywell Deutschland AG, Neunkirchen) | ||
| + | <li>N,N,N͛,N͛-Tetramethylethylendiamin (TEMED) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Natriumacetat (AppliChem GmbH, Darmstadt) | ||
| + | <li>Natriumcarbonat (Na2CO3) (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | <li>Natriumchlorid (NaCl) (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | <li>Natriumdihydrogenphosphat (NaH2PO4) (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | <li>Dinatriumhydrogenphosphat (Na2HPO4) (VWR International GmbH, Ismaning) | ||
| + | <li>Natriumdodecylsulfat (SDS) (Serva Electrophoresis GmbH, Heidelberg) | ||
| + | <li>Natriumhydroxid (NaOH) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Nickelsulfat (Ni(II)SO4· 6 H2O) (AppliChem GmbH, Darmstadt) | ||
| + | <li>L-Prolin (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | <li>Puffer-Standardlösungen für pH-Meter (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | <li>Salzsäure, konz. (HCl) (Honeywell Deutschland AG, Neunkirchen) | ||
| + | <li>Tris-(hydroxymethyl)-aminomethan (Tris) (AppliChem GmbH, Darmstadt) | ||
| + | <li>Tween 20 SigmaUltra (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Xylencyanol (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Zinksulfat (ZnSO4· 7 H2O) (Carl Roth GmbH & Co. KG, Karlsruhe) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ==Standards== | ||
| + | ===DNA standars=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>GeneRuler 1 kb DNA Ladder (Fermentas GmbH, St. Leon-Rot) | ||
| + | <li>GeneRuler 100 bp DNA Ladder (Fermentas GmbH, St. Leon-Rot) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ===Proteinstandard for SDS-PAGE=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>Unstained Protein MW Marker (Fermentas GmbH, St. Leon-Rot) | ||
| + | <li>PageRuler Prestained Protein Ladder (Fermentas GmbH, St. Leon-Rot) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ===Proteinstandard for size exclusion chromatography=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>Aprotinin; 6,5 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Cytochrom C; 12,4 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Carboanhydrase; 29 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Rinderserumalbumin; 66 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>β-Amylase; 200 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Apoferritin; 443 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Thyroglobulin 669 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>Dextranblau 2000 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ===Proteinstandard for isoelectric focussing=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>IEF mix 3.6-9.3 (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ==Kits== | ||
| + | ===Kits for molecular biology work=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>QIAprep Spin Miniprep Kit (Qiagen GmbH, Hilden) | ||
| + | <li>QIAquick Gel Extraction Kit (Qiagen GmbH, Hilden) | ||
| + | <li>QIAquick PCR Purification Kit (Qiagen GmbH, Hilden) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ===Kits for protein quantification=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>Dual-Luciferase Reporter Assay (Promega GmbH, Mannheim) | ||
| + | <li>BTS-Tabletten (Roche Deutschland Holding GmbH, Grenzach-Wyhlen) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | ===Kit for DNA sequencing=== | ||
| + | <div class="white-box"> | ||
| + | <ul> | ||
| + | <li>Big Dye Terminator Cycle Sequencing (Life Technologies GmbH, Darmstadt) | ||
| + | <li>TSR-Puffer = Formamid GC-grade (Sigma-Aldrich Chemie GmbH, Taufkirchen) | ||
| + | <li>ZR DNA Sequencing Clean-up Kit (Zymo Research Europe GmbH, Freiburg) | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <references /> | ||
| + | |||
| + | {{LMU-TUM_Munich_html_end}} | ||
Latest revision as of 16:43, 30 November 2016
Materials
Enzymes and Proteins
Enzymes
- Lysozym (22770 U/mg) (AppliChem GmbH, Darmstadt)
- Pfu-Ultra II DNA Polymerase (Promega GmbH, Mannheim)
- T4-DNA-Ligase (200 CEU/μl) (Fermentas GmbH, St. Leon-Rot)
- Alkalische Phosphatase aus der arktischen Garnele (1 U/μl)(Thermo Fisher Scientific Inc., St. Leon-Rot)
- FastAP thermosensitive alkalische Phosphatase (1 U/μl) (Thermo Fisher Scientific Inc., St. Leon-Rot)
Restriction enzymes
- AfeI / Eco47III (Thermo Fisher Scientific Inc., St. Leon-Rot)
- HindIII (Thermo Fisher Scientific Inc., St. Leon-Rot)
- DpnI (Thermo Fisher Scientific Inc., St. Leon-Rot)
- MlyI (New England Biolabs GmbH, Frankfurt am Main)
- NcoI (Fermentas GmbH, St. Leon-Rot)
- NheI (Fermentas GmbH, St. Leon-Rot)
- SapI (New England Biolabs GmbH, Frankfurt am Main)
- StuI / Eco147I (Fermentas GmbH, St. Leon-Rot)
- NgoMIV (New England Biolabs GmbH, Frankfurt am Main)
- XbaI (Thermo Fisher Scientific Inc., St. Leon-Rot)
Proteins
Albumin Fraktion V (BSA) (AppliChem GmbH, Darmstadt)
Chemicals
- 2,2'-Dithiodipyridin (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- 2-Mercaptoethanol (AppliChem GmbH, Darmstadt)
- Acetonitril (LC-MS grade) (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- 30% Acrylamid mit 0,8% Bisacrylamid (Serva Electrophoresis GmbH, Heidelberg)
- Agarose (peqlab Biotechnologie GmbH, Erlangen)
- Agarose (low melting point) (InvitrogenAG, Basel, Schweiz)
- Ameisensäure (LC-MS grade) (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Ammoniak, 25 %ig (Honeywell Deutschland AG, Neunkirchen)
- Ammoniumacetat (AppliChem GmbH, Darmstadt)
- Ammoniumchloris (NH4Cl) (AppliChem GmbH, Darmstadt)
- Ammoniumperoxodisulfat (APS) (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Ampicillin, Natriumsalz (Formedium, Hunstanton, UK)
- Anhydrotetrazyklin (aTc) (Acros Oranics, Geel, Belgien)
- Antifoam A, 30 %ige Stammlösung (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Bacto Agar Becton (DIFCO Laboratories GmbH, Augsburg)
- Bacto Tryptone Becton (BD, DIFCO Laboratories GmbH, Augsburg)
- Bacto Yeast Extract Becton (BD, DIFCO Laboratories GmbH, Augsburg)
- Betain, Monohydrat (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Borsäure (AppliChem GmbH, Darmstadt)
- Bromphenolblau (AppliChem GmbH, Darmstadt)
- Coomassie Brilliantblau R-250 (AppliChem GmbH, Darmstadt)
- Chloramphenicol (AppliChem GmbH, Darmstadt)
- D(+)-Glukose, Monohydrat (AppliChem GmbH, Darmstadt)
- D(+)-Saccharose (AppliChem GmbH, Darmstadt
- Dimethylformamid (DMF) (Merck KG, Darmstadt)
- Dithiodiethanol (Acros Oranics, Geel, Belgien)
- dNTPs (InvitrogenAG, Basel, Schweiz)
- Essigsäure, 99,9 % (Honeywell Deutschland AG, Neunkirchen)
- Ethanol p. a., 99,9 % (Honeywell Deutschland AG, Neunkirchen)
- Ethidiumbromid (AppliChem GmbH, Darmstadt)
- Ethylendinitrilo-tetraessigsäure-Na2 (EDTA) (AppliChem GmbH, Darmstadt)
- L-Glycin (Carl Roth GmbH & Co. KG, Karlsruhe)
- Glycerin (Carl Roth GmbH & Co. KG, Karlsruhe)
- Guanidiniumchlorid (Nigu Chemie GmbH, Waldkraiburg)
- Harnstoff (CH4N2O) (AppliChem GmbH, Darmstadt)
- Helipur H plus N (B. Braun Melsungen AG, Melsungen)
- Imidazol (Merck KG, Darmstadt)
- Isopropanol (Honeywell Deutschland AG, Neunkirchen)
- Kaliumchlorid (KCl) (Merck KG, Darmstadt)
- Kaliumdihydrogenphosphat (KH2PO4) (AppliChem GmbH, Darmstadt)
- Kaliumsulfat (K2SO4) (AppliChem GmbH, Darmstadt)
- Magnesiumchlorid (MgCl2 · 6 H2O) (AppliChem GmbH, Darmstadt)
- Methanol (Honeywell Deutschland AG, Neunkirchen)
- N,N,N͛,N͛-Tetramethylethylendiamin (TEMED) (AppliChem GmbH, Darmstadt)
- Natriumacetat (AppliChem GmbH, Darmstadt)
- Natriumcarbonat (Na2CO3) (Carl Roth GmbH & Co. KG, Karlsruhe)
- Natriumchlorid (NaCl) (Carl Roth GmbH & Co. KG, Karlsruhe)
- Natriumdihydrogenphosphat (NaH2PO4) (Carl Roth GmbH & Co. KG, Karlsruhe)
- Dinatriumhydrogenphosphat (Na2HPO4) (VWR International GmbH, Ismaning)
- Natriumdodecylsulfat (SDS) (Serva Electrophoresis GmbH, Heidelberg)
- Natriumhydroxid (NaOH) (AppliChem GmbH, Darmstadt)
- Nickelsulfat (Ni(II)SO4· 6 H2O) (AppliChem GmbH, Darmstadt)
- L-Prolin (Carl Roth GmbH & Co. KG, Karlsruhe)
- Puffer-Standardlösungen für pH-Meter (Carl Roth GmbH & Co. KG, Karlsruhe)
- Salzsäure, konz. (HCl) (Honeywell Deutschland AG, Neunkirchen)
- Tris-(hydroxymethyl)-aminomethan (Tris) (AppliChem GmbH, Darmstadt)
- Tween 20 SigmaUltra (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Xylencyanol (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Zinksulfat (ZnSO4· 7 H2O) (Carl Roth GmbH & Co. KG, Karlsruhe)
Standards
DNA standars
- GeneRuler 1 kb DNA Ladder (Fermentas GmbH, St. Leon-Rot)
- GeneRuler 100 bp DNA Ladder (Fermentas GmbH, St. Leon-Rot)
Proteinstandard for SDS-PAGE
- Unstained Protein MW Marker (Fermentas GmbH, St. Leon-Rot)
- PageRuler Prestained Protein Ladder (Fermentas GmbH, St. Leon-Rot)
Proteinstandard for size exclusion chromatography
- Aprotinin; 6,5 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Cytochrom C; 12,4 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Carboanhydrase; 29 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Rinderserumalbumin; 66 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- β-Amylase; 200 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Apoferritin; 443 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Thyroglobulin 669 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- Dextranblau 2000 kDa (Sigma-Aldrich Chemie GmbH, Taufkirchen)
Proteinstandard for isoelectric focussing
- IEF mix 3.6-9.3 (Sigma-Aldrich Chemie GmbH, Taufkirchen)
Kits
Kits for molecular biology work
- QIAprep Spin Miniprep Kit (Qiagen GmbH, Hilden)
- QIAquick Gel Extraction Kit (Qiagen GmbH, Hilden)
- QIAquick PCR Purification Kit (Qiagen GmbH, Hilden)
Kits for protein quantification
- Dual-Luciferase Reporter Assay (Promega GmbH, Mannheim)
- BTS-Tabletten (Roche Deutschland Holding GmbH, Grenzach-Wyhlen)
Kit for DNA sequencing
- Big Dye Terminator Cycle Sequencing (Life Technologies GmbH, Darmstadt)
- TSR-Puffer = Formamid GC-grade (Sigma-Aldrich Chemie GmbH, Taufkirchen)
- ZR DNA Sequencing Clean-up Kit (Zymo Research Europe GmbH, Freiburg)
