Difference between revisions of "Team:Paris Bettencourt/Description"

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{{Paris_Bettencourt}}
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{{Paris_Bettencourt/Menu}}
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{{Paris_Bettencourt/Wiki}}
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                                            <!-- Code by the 2016 Paris Bettencourt -->
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<h3>★  ALERT! </h3>
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position : relative;
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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margin-top : -120px;
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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background-size : cover;
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<div class="column full_size">
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line-height : normal;
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text-align : center;
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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.layout {
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<ul>
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width : 100%;
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<li> A clear and concise description of your project.</li>
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height : 100%;
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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z-index : 90;
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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</div>
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<div class="column full_size" >
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<h5>Advice on writing your Project Description</h5>
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<p>
 
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
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</p>
 
  
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<p>
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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</p>
 
  
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</div>
 
  
  
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<div class="column half_size" >
 
  
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<h5>References</h5>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
  
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</style>
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<head>
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</head>
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<body>
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<div id=topheader>
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<div id=video >
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<video width="800" height="450" controls>
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  <source src="https://static.igem.org/mediawiki/2016/9/98/Paris_Bettencourt-video.mp4" type="video/mp4">
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</video>
 
</div>
 
</div>
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</div>
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<div id=subheader>
  
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<div style="margin-top:20px; margin-bottom:20px">
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<div class="panel" >
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<a href="https://2016.igem.org/Team:Paris_Bettencourt/Project/Assay" title="Assay">
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  <img class="narrowimg" src="https://static.igem.org/mediawiki/2016/b/b9/Paris_Bettencourt-Assay_Button2.png" width="150px" height="250px"/>
 +
  <div class="titlebox">
 +
    <center><img src="https://static.igem.org/mediawiki/2016/e/e8/Paris_Bettencourt-Logo_Assay.png" style="height:60px;"/></center>
 +
    <div style="width:60%;margin-left:20%;margin-bottom:20px;"><hr></div>
 +
    Assay
 +
  </div>
 +
</div>
 +
      </a>
 +
      <a href="https://2016.igem.org/Team:Paris_Bettencourt/Project/Microbiology" title="Microbiology">
 +
<div id="dspanel" class="subpanel2">
 +
  <img class="narrowimg" src="https://static.igem.org/mediawiki/2016/e/e0/Paris_Bettencourt-Microbiology_Button2.png" width="150px" height="250px"/>
 +
  <div class="titlebox">
 +
    <center><img src="https://static.igem.org/mediawiki/2016/f/f8/Paris_Bettencourt-Logo_Microbiology.png" style="height:60px;"/></center>
 +
    <div style="width:60%;margin-left:20%;margin-bottom:20px;"><hr></div>
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    Microbiology
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  </div>
 +
</div>
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      </a>
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      <a href="https://2016.igem.org/Team:Paris_Bettencourt/Project/Enzyme" title="Enzyme">
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<div id="tcpanel" class="subpanel2">
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  <img class="narrowimg" src="https://static.igem.org/mediawiki/2016/d/d0/Paris_Bettencourt-Enzyme_Button2.png" width="150px" height="250px"/>
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  <div class="titlebox">
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    <center><img src="https://static.igem.org/mediawiki/2016/9/9d/Paris_Bettencourt-Logo_Enzyme.png" style="height:60px;"/></center>
 +
    <div style="width:60%;margin-left:20%;margin-bottom:20px;"><hr></div>
 +
  Enzyme
 +
  </div>
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</div>
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      </a>
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      <a href="https://2016.igem.org/Team:Paris_Bettencourt/Project/Binding" title="Binding domains">
 +
<div id="thpanel" class="subpanel2">     
 +
  <img class="narrowimg" src="https://static.igem.org/mediawiki/2016/6/68/Paris_Bettencourt-Binding_Button2.png" width="150px" height="250px"/>
 +
  <div class="titlebox">
 +
    <center><img src="https://static.igem.org/mediawiki/2016/4/44/Paris_Bettencourt-Logo_Binding.png" style="height:60px;"/></center>
 +
    <div style="width:60%;margin-left:20%;margin-bottom:20px;"><hr></div>
 +
    Binding
 +
  </div>
 +
</div>
 +
      </a>
 +
    <a href="https://2016.igem.org/Team:Paris_Bettencourt/Project/Indigo" title="Indigo">
 +
<div id="thpanel" class="subpanel2">     
 +
  <img class="narrowimg" src="https://static.igem.org/mediawiki/2016/7/75/Paris_Bettencourt-Indigo_Button2.png" width="150px" height="250px"/>
 +
  <div class="titlebox">
 +
    <center><img src="https://static.igem.org/mediawiki/2016/f/f0/Paris_Bettencourt-Logo_Indigo.png" style="height:60px;"/></center>
 +
    <div style="width:60%;margin-left:20%;margin-bottom:10px;"><hr></div>
 +
    Indigo
 +
  </div>
 +
</div>
 +
      </a>
 +
<a href="https://2016.igem.org/Team:Paris_Bettencourt/Model" title="Model">
 +
<div id="thpanel" class="subpanel2">     
 +
  <img class="narrowimg" src="https://static.igem.org/mediawiki/2016/9/96/Paris_Bettencourt-Model_Button2.png" width="150px" height="250px"/>
 +
  <div class="titlebox">
 +
    <center><img src="https://static.igem.org/mediawiki/2016/2/21/Paris_Bettencourt-Model_Icon.png" style="height:60px;"/></center>
 +
    <div style="width:60%;margin-left:20%;margin-bottom:10px;"><hr></div>
 +
    Model
 +
  </div>
 +
</div>
 +
      </a>
 +
</div>
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</div>
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<div id=definition>
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<h2 class="red">Short Summary: What We Did</h2>
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<p class=text style="font-size: 17px;">
  
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<div class="column half_size" >
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<br>In this project we <a href="https://2016.igem.org/Team:Paris_Bettencourt/Project/Enzyme">produced synthetic enzymes</a> to remove red wine stains from fabric. These enzymes are designed to replace perchloroethylene (PERC), a toxic solvent used in dry cleaning that will soon be banned in France.<br>
−
<h5>Inspiration</h5>
+
 
−
<p>See how other teams have described and presented their projects: </p>
+
<br><a href="https://2016.igem.org/Team:Paris_Bettencourt/Integrated_Practices">We listened to people</a> who will use our product, conducting face-to-face interviews with every single dry cleaner in Paris. From this came a plan for a realistic product, a stain fighting enzymatic pretreatment compatible with existing cleaning technologies and workflows.<br>
 +
 
 +
<br><a href="https://2016.igem.org/Team:Paris_Bettencourt/Project/Microbiology">We isolated microbes</a> capable of degrading red wine from a library of 186 strains taken from vineyard soil samples collected around the world with the help of our fellow iGEMers. The most effective microbes were submitted for whole genome resequencing, then analyzed to produce a short list of candidate stain-fighting enzymes.<br>
 +
 
 +
<br><a href = "https://2016.igem.org/Team:Paris_Bettencourt/Model">We modeled enzymatic activity at the fabric surface</a> and determined that activity could be substantially improved if the enzymes had a moderate binding affinity for the fabric itself. This effectively increases the enzyme concentration at the fabric surface and reduces the quantity of enzymes lost through diffusion into the medium.<br>
 +
 
 +
<br><a href = "https://2016.igem.org/Team:Paris_Bettencourt/Project/Binding">We identified short peptides</a> with affinity to cotton, linen, wool, polyester and silk using the method of phage display. The resulting Fabric Binding Domains (FBDs) were quantitatively characterized using ELISA, to determine peptides with optimal affinity as determined by our model.<br>
 +
 
 +
<br><a href = "https://2016.igem.org/Team:Paris_Bettencourt/Project/Assay">We developed a high-throughput assay</a> to quantify stain removal on real cloth. Laser-cut fabric samples are sealed to 96-well microplates and imaged on flat-bed scanners. We coded custom image analysis software to identify each circular fabric sample and measure the stain intensity. <br>
 +
 
 +
<br><a href = "https://2016.igem.org/Team:Paris_Bettencourt/Parts">We constructed new BioBricks</a>, fusions of our most promising fabric binding domains to our favorite wine-degrading enzymes. The resulting proteins were expressed, purified and characterized both in vitro (in solution) and in situ (on real stained fabric).<br>
 +
 
 +
<br> We hope these pages convince you that our project is mature, thoroughly documented and effective. We believe that product is ready solve one small problem that real people have every day. <br>
 +
 
 +
 
 +
</p>
  
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<ul>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
−
<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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</ul>
 
 
</div>
 
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{{Paris_Bettencourt/Footer}}

Latest revision as of 10:59, 1 December 2016


Short Summary: What We Did


In this project we produced synthetic enzymes to remove red wine stains from fabric. These enzymes are designed to replace perchloroethylene (PERC), a toxic solvent used in dry cleaning that will soon be banned in France.

We listened to people who will use our product, conducting face-to-face interviews with every single dry cleaner in Paris. From this came a plan for a realistic product, a stain fighting enzymatic pretreatment compatible with existing cleaning technologies and workflows.

We isolated microbes capable of degrading red wine from a library of 186 strains taken from vineyard soil samples collected around the world with the help of our fellow iGEMers. The most effective microbes were submitted for whole genome resequencing, then analyzed to produce a short list of candidate stain-fighting enzymes.

We modeled enzymatic activity at the fabric surface and determined that activity could be substantially improved if the enzymes had a moderate binding affinity for the fabric itself. This effectively increases the enzyme concentration at the fabric surface and reduces the quantity of enzymes lost through diffusion into the medium.

We identified short peptides with affinity to cotton, linen, wool, polyester and silk using the method of phage display. The resulting Fabric Binding Domains (FBDs) were quantitatively characterized using ELISA, to determine peptides with optimal affinity as determined by our model.

We developed a high-throughput assay to quantify stain removal on real cloth. Laser-cut fabric samples are sealed to 96-well microplates and imaged on flat-bed scanners. We coded custom image analysis software to identify each circular fabric sample and measure the stain intensity.

We constructed new BioBricks, fusions of our most promising fabric binding domains to our favorite wine-degrading enzymes. The resulting proteins were expressed, purified and characterized both in vitro (in solution) and in situ (on real stained fabric).

We hope these pages convince you that our project is mature, thoroughly documented and effective. We believe that product is ready solve one small problem that real people have every day.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
igem2016parisbettencourt@gmail.com
2016.igem.org