Difference between revisions of "Team:UofC Calgary"

 
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{{Team:UofC_Calgary/assets/plugins/cubeportfolio/css/cubeportfolio.min.css}}
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{{Team:UofC_Calgary/assets/plugins/owl-carousel/owl.transitions.css}}
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{{Team:UofC_Calgary/assets/plugins/fancybox/jquery.fancybox.css}}
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{{Team:UofC_Calgary/assets/base/css/themes/default.css}}
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{{Team:UofC_Calgary/assets/base/css/custom.css}}
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{{Team:UofC_Calgary/assets/google-fonts.css}}
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{{Team:UofC_Calgary/assets/plugins/jquery.min.js}}
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{{Team:UofC_Calgary/assets/plugins/jquery-migrate.min.js}}
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{{Team:UofC_Calgary/assets/plugins/bootstrap/js/bootstrap.min.js}}
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{{Team:UofC_Calgary/assets/plugins/cubeportfolio/js/jquery.cubeportfolio.min.js}}
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{{Team:UofC_Calgary/assets/plugins/owl-carousel/owl.carousel.min.js}}
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{{Team:UofC_Calgary/assets/plugins/counterup/jquery.waypoints.min.js}}
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</div>
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<!-- BEGIN HEAD -->
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<head>
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<meta charset="utf-8"/>
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<title>iGEM Calgary 2016</title>
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<meta http-equiv="X-UA-Compatible" content="IE=edge">
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<meta content="width=device-width, initial-scale=1.0" name="viewport"/>
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<meta http-equiv="Content-type" content="text/html; charset=utf-8">
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<meta content="" name="description"/>
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<meta content="" name="author"/>
  
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<div class="column full_size" >
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<link rel="shortcut icon" href="favicon.ico"/>
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<h2> Welcome to iGEM 2016! </h2>
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</head>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<body class="c-layout-header-fixed">
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<!-- BEGIN: LAYOUT/HEADERS/HEADER-1 -->
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<!-- BEGIN: HEADER -->
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<header class="c-layout-header c-layout-header-3 c-layout-header-default-mobile c-header-seethrough" style="padding-top: 10px;
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    z-index: 9995;
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    width: 100%;
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">
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<div class="c-navbar">
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<div class="container-fluid" style="padding-left: 20px; padding-right: 20px;">
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<!-- BEGIN: BRAND -->
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<div class="c-navbar-wrapper clearfix">
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<div class="c-brand c-pull-left">
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<a href="https://2016.igem.org/Team:UofC_Calgary" class="c-logo">                                         
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<img src="https://static.igem.org/mediawiki/2016/9/9a/Logo-1.png" alt="iGEM" class="c-desktop-logo">
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<img src="https://static.igem.org/mediawiki/2016/9/9a/Logo-1.png" alt="iGEM" class="c-desktop-logo-inverse">
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<img src="https://static.igem.org/mediawiki/2016/9/9a/Logo-1.png" alt="iGEM" class="c-mobile-logo">
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</a>
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<button class="c-hor-nav-toggler" type="button" data-target=".c-mega-menu">
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<span class="c-line"></span>
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</button>
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<!-- BEGIN: HOR NAV -->
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<!-- BEGIN: MEGA MENU -->
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<nav class="c-mega-menu c-pull-right c-mega-menu-dark c-mega-menu-dark-mobile c-fonts-uppercase c-fonts-bold">
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<ul class="nav navbar-nav c-theme-nav">
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<li class="c-active c-menu-type-classic">
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<a href="https://2016.igem.org/Team:UofC_Calgary" class="c-link dropdown-toggle">Home</a>
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</li>
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<li class="c-menu-type-classic">
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<a href="https://2016.igem.org/Team:UofC_Calgary/Description" class="c-link dropdown-toggle">Project</a>
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<ul class="dropdown-menu c-menu-type-inline c-pull-left">
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Description">Problem</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Experiments">Our Experiments</a>
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</li>
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                                                                <li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Results">Our Results</a>
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</li>
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                                                                <li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Model">Model</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Design">Applied Design</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Parts">Parts Registry</a>
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                                                                <li>
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                                                                <a href="https://2016.igem.org/Team:UofC_Calgary/Composite_Part">Composite Parts</a>
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</li>
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</li>
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</ul>
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</li>
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<li class="c-menu-type-classic">
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<a href="https://2016.igem.org/Team:UofC_Calgary/Judging" class="c-link">Judging</a>
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</li>
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<li class="c-menu-type-classic">
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<a href="https://2016.igem.org/Team:UofC_Calgary/Human_Practices" class="c-link dropdown-toggle">Human Practices</a>
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<ul class="dropdown-menu c-menu-type-inline c-pull-left">
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Integrated_Practices">Integrated Practices</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Collaborations">Collaborations</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Engagement">Engagement</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Safety">Safety</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/HP/Silver">Silver</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/HP/Gold">Gold</a>
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</li>
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<li>
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                                    <a href="https://2016.igem.org/Team:UofC_Calgary/Policy"> Policy Brief </a>
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                                    </li>
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</ul>
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</li>
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                                <li class="c-menu-type-classic">
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<a href="https://2016.igem.org/Team:UofC_Calgary/Notebook" class="c-link">Notebook</a>
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</li>
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<li class="c-menu-type-classic">
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<a href="https://2016.igem.org/Team:UofC_Calgary/Team" class="c-link dropdown-toggle">Team</a>
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<ul class="dropdown-menu c-menu-type-inline c-pull-left">
 +
                                                                <li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Team">Meet Our Team</a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Attributions">Attributions</a>
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</li>
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 +
</ul>
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</li>
  
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</div>  
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<li>
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<a href="https://2016.igem.org/Main_Page" data-toggle="modal" data-target="#login-form" class="c-btn-border-opacity-04 c-btn btn-no-focus c-btn-header btn btn-sm c-btn-border-1x c-btn-white c-btn-circle c-btn-sbold"><i class="icon-chemistry"></i> iGEM </a>
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<div class="column half_size" >
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</li>
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<h5>Before you start: </h5>
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<li class="c-quick-sidebar-toggler-wrapper">
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<p> Please read the following pages:</p>
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<a href="#" class="c-quick-sidebar-toggler">
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<ul>
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<span class="c-line"></span>
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<li> <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<span class="c-line"></span>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<span class="c-line"></span>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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</a>
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</ul>
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</li>
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</ul>
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<!-- END MEGA MENU -->
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</nav>
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<!-- END: MEGA MENU -->
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<!-- END: LAYOUT/HEADERS/MEGA-MENU -->
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<!-- END: HOR NAV -->
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</div>
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</div>
 
</div>
 
</div>
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</header>
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<div class="column half_size" >
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<!-- END: HEADER -->
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<div class="highlight">
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<!-- END: LAYOUT/HEADERS/HEADER-1 -->
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<h5> Styling your wiki </h5>
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<!-- BEGIN: LAYOUT/SIDEBARS/QUICK-SIDEBAR -->
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<nav class="c-layout-quick-sidebar">
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<div class="c-header">
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<button type="button" class="c-link c-close">
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<i class="icon-login"></i>
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</button>
 
</div>
 
</div>
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<div class="c-content">
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<div class="c-section">
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<h3>Theme Colors</h3>
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<div class="c-settings">
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<span class="c-color c-default c-active" data-color="default"></span>
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<span class="c-color c-green1" data-color="green1"></span>
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<span class="c-color c-green2" data-color="green2"></span>
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<span class="c-color c-green3" data-color="green3"></span>
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<span class="c-color c-yellow1" data-color="yellow1"></span>
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<span class="c-color c-yellow2" data-color="yellow2"></span>
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<span class="c-color c-yellow3" data-color="yellow3"></span>
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<span class="c-color c-red1" data-color="red1"></span>
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<span class="c-color c-red2 " data-color="red2"></span>
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<span class="c-color c-red3" data-color="red3"></span>
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<span class="c-color c-purple1" data-color="purple1"></span>
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<span class="c-color c-purple2" data-color="purple2"></span>
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<span class="c-color c-purple3" data-color="purple3"></span>
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<span class="c-color c-blue1" data-color="blue1"></span>
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<span class="c-color c-blue2" data-color="blue2"></span>
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<span class="c-color c-blue3" data-color="blue3"></span>
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<span class="c-color c-brown1" data-color="brown1"></span>
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<span class="c-color c-brown2" data-color="brown2"></span>
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<span class="c-color c-brown3" data-color="brown3"></span>
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<span class="c-color c-dark1" data-color="dark1"></span>
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<span class="c-color c-dark2" data-color="dark2"></span>
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<span class="c-color c-dark3" data-color="dark3"></span>
 +
</div>
 +
</div>
 +
<div class="c-section">
 +
<h3>Header Type</h3>
 +
<div class="c-settings">
 +
<input type="button" class="c-setting_header-type btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase active" data-value="boxed" value="boxed"/>
 +
<input type="button" class="c-setting_header-type btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase" data-value="fluid" value="fluid"/>
 +
</div>
 +
</div>
 +
<div class="c-section">
 +
<h3>Header Mode</h3>
 +
<div class="c-settings">
 +
<input type="button" class="c-setting_header-mode btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase active" data-value="fixed" value="fixed"/>
 +
<input type="button" class="c-setting_header-mode btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase" data-value="static" value="static"/>
 +
</div>
 +
</div>
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<div class="c-section">
 +
<h3>Mega Menu Style</h3>
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<div class="c-settings">
 +
<input type="button" class="c-setting_megamenu-style btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase active" data-value="dark" value="dark"/>
 +
<input type="button" class="c-setting_megamenu-style btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase" data-value="light" value="light"/>
 +
</div>
 +
</div>
 +
<div class="c-section">
 +
<h3>Font Style</h3>
 +
<div class="c-settings">
 +
<input type="button" class="c-setting_font-style btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase active" data-value="default" value="default"/>
 +
<input type="button" class="c-setting_font-style btn btn-sm c-btn-square c-btn-border-1x c-btn-white c-btn-sbold c-btn-uppercase" data-value="light" value="light"/>
 +
</div>
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</div>
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</div>
 +
</nav>
 +
<!-- END: LAYOUT/SIDEBARS/QUICK-SIDEBAR -->
 +
<!-- BEGIN: PAGE CONTAINER -->
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<div class="c-layout-page" style="padding-top: 88px;">
 +
<!-- BEGIN: PAGE CONTENT -->
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<!-- BEGIN: LAYOUT/SLIDERS/REVO-SLIDER-1 -->
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<!--BEGIN: SLIDE #1 -->
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            <img alt="" src="https://static.igem.org/mediawiki/2016/7/78/T--UofC_Calgary--bgmin.jpg" data-bgposition="center center" data-bgfit="cover" data-bgrepeat="no-repeat" style="width: 100%; height: 100%;">
 +
<!-- END: LAYOUT/SLIDERS/REVO-SLIDER-1 -->
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<div class="c-content-box c-size-md" style="background-color: #243338; padding-bottom:20px;">
 +
  <div class="container">
 +
<div class="c-content-title-1">
 +
<h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Giant Jamboree Results</h3>
 +
<div class="c-line-center c-theme-bg">
 +
</div>
 +
</div>
 +
                <div class="container" style="margin-right: 0%">
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                        <div class="row">
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                            <div class="col-md-12 c-no-bottom-padding">
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 +
                                            <p style="color: #FFFFFF">We are honoured to announce that the UofC_Calgary 2016 iGEM was incredibly successful at this year's iGEM Giant Jamboree. We received a <u>gold medal</u>, a nomination for <u>Best Applied Design</u>, and the award for <u>Best Integrated Human Practices</u>! We are beyond thrilled to continue the University of Calgary's tradition of excellence at iGEM, and extend our deepest gratitude to those who aided us in any form during this journey.
 +
</p><div class="col-md-4"><center><img src="https://static.igem.org/mediawiki/2016/a/a3/T--UofC_Calgary--Gold%28%2B%29.png" style="width:20vw;" alt="Gold Medal didn't load. Please Refresh Page."></center></div><div class="col-md-8" style="padding-top: 50px;"><center> <img width="500px" height="375px" src="https://static.igem.org/mediawiki/2016/5/5a/T--UofC_Calgary--boston_photo.jpg"></center>
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</div>
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                                </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 
</div>
 
</div>
  
−
<div class="column full_size" >
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<div class="c-content-box c-size-md c-bg-darkblue c-no-bottom-padding homepage1">
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<h5> Wiki template information </h5>
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                        <div class="c-content-title-1">
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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                            <h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Our Project</h3>
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                            <div class="c-line-center c-theme-bg"></div>
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                        </div>
 +
                <div class="container" style="margin-right: 0%">
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                    <div class="c-content-product-1 c-opt-1">
 +
 +
                        <div class="row">
 +
                            <div class="col-md-10 pull-right c-no-bottom-padding">
 +
                                <div class="c-body realdiv" style="
 +
    padding: 0px 20px 20px;">
 +
                                            <p style="color: #FFFFFF">Mars and the cosmos are a tantalizingly close goal for Mankind to reach in the next century; however, there are several glaring problems that we must overcome to reach such an objective. Radiation is chief among them. On Earth, individuals annually receive 2.4 mSv of ionizing radiation (IR), which is easily tolerated by the human body. Unfortunately, without the protection of the magnetosphere, astronauts in space are exposed to high levels of IR in the range of 50 to 2000 mSv. This level of radiation causes the accumulation of deleterious double strand breaks in DNA. Despite current research into methods of IR protection, many solutions such as radiation shield coating are quite expensive, especially to transport to space. Even existing biological solutions are insufficient, such as the use of ingestible or injectable radioprotectors, which are subject to sinusuoidal pharmacokinetics. Our project is based on the administration of the naturally occuring peptide Bowman-Birk Protease Inhibitor (BBI), which has been shown to confer protection against DNA damage following radiation exposure. (Dittmann <i>et al.</i>, 2003). </p>
  
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</div>
 
  
 +
<p style="color: #FFFFFF">References</p>
  
 +
<p style="color: #FFFFFF"><font size="2">Dittmann, K.H., Mayer, C., and Rodemann, H.P. (2003). Radioprotection of normal tissue to improve radiotherapy: the effect of the Bowman Birk protease inhibitor. <i>Current Medicinal Chemistry - Anticancer Agents, 3</i>(5), 360-363.</font </p>
  
  
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<div class="column half_size" >
 
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<h5> Editing your wiki </h5>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
−
<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
 
  
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                                </div>
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 
</div>
 
</div>
 +
<!-- BEGIN: CHASSIS -->
 +
<div class="c-content-box c-size-md yellowdiv c-no-bottom-padding homepage2">
 +
                        <div class="c-content-title-1">
 +
                            <h3 class="c-center c-font-uppercase c-font-bold" style="color:#273236 !important">OUR CHASSIS</h3>
 +
                            <div class="c-line-center c-theme-bg"></div>
 +
                        </div>
 +
<div class="container" style="margin-left: 0%">
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                                            <p style="color: #273236">To express mBBI, we had to choose a biological system that was not only capable of high expression but was robust, adaptable, and easily genetically manipulated for ease of transport and use in remote and extreme environments. After creating a list of different types of cells which could be used for the expression of our peptide, we found that <i>Bacillus subtilis</i> was especially suited for our patch design. This is due to many reasons. <i>B. subtilis</i> is able to express high amounts of recombinant protein which can be secreted into surrounding media by its own well-characterized secretion system, making it ideal for producing and delivering the correct dosage of mBBI into the user. <i>B. subtilis</i> is capable of forming spores—which have been found in arctic permafrost dating tens of thousands of years and yet are still viable—making it easy to store in a desiccated or frozen manner. Additionally, <i>B. subtilis</i> expresses high amounts of recombinase A, a protein which allows genes to be stably integrated into the chromosome.</p>
  
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<div class="column half_size" >
+
<p style="color: #273236">Our strain of <i>B. subtilis</i> (WB800) is particularly suited for high production because it is deficient in eight extracellular proteases which could lead to the premature degradation our radioprotective peptide. Our strain will allow the peptide to be stable in the media within the patch. We conducted tests on the growth patterns of <i>B. subtilis</i> in the novel environment of a patch and optimized patch media to simulate an ideal growth conditions. We have also designed a genetic construct which provides inducible competency to the <i>B. subtilis</i> strain upon the addition of xylose, making this bacterium much easier to work with for future teams. </p>  
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<h5>Tips</h5>
+
                                </div>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
                            </div>
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<ul>
+
                        </div>
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
                    </div>
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
                </div>
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
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</ul>  
+
 
</div>
 
</div>
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<!-- BEGIN: DEVICE -->
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<div class="c-content-box c-size-md c-bg-darkblue c-no-bottom-padding homepage3">
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                        <div class="c-content-title-1">
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<h5>Inspiration</h5>
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                            <h3 class="c-center c-font-uppercase c-font-bold" style="color:#FFFFFF">Design Overview</h3>
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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                            <div class="c-line-center c-theme-bg"></div>
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<ul>
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                        </div>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<div class="container" style="margin-right: 0%">
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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                    <div class="c-content-product-1 c-opt-1">
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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                        <div class="row">
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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                                <div class="c-body realdiv" style="
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    padding: 0px 20px 20px;">
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                                        <br> <br> <br> <br> <br><p style="color: #FFFFFF">We designed our project to overcome common issues in existing technologies such as cost, portability, and durability. Our patch is portable, lightweight, safe to use, can be stored long-term, and produces and delivers peptides at a steady rate to maintain a constant concentration in the body. We worked with professionals within NASA and the CSA to integrate our solution with current space travel infrastructure, and with companies specializing in transdermal delivery to optimize the patch system. We also consulted with professionals and researchers in biomedical fields to better understand the unknown effects our delivery system would have when in use. Using this system, our project has future applications here on Earth for other occupations and individuals exposed to high levels of ionizing radiation, such as pilots, flight attendants, and miners. Our project can also be used to deliver hormones and other small molecules for use in space, serving as a "biological pharmacy." </p> <br> <br> <br> <br> <br> <br> <br> <br> <br>  
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                                </div>
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                            </div>
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                        </div>
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                    </div>
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                </div>
 
</div>
 
</div>
  
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<div class="column half_size" >
 
−
<h5> Uploading pictures and files </h5>
 
−
<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
−
When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
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<!-- BEGIN: CONTENT/FEATURES/FEATURES-1 -->
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    </div>
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<!-- BEGIN: FEATURES 1 -->
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<div style="background-color="#172724">
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        <a href="https://2016.igem.org/Team:UofC_Calgary/Policy">
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        <img style="width:100%;" src="https://static.igem.org/mediawiki/2016/5/5a/PolicyBriefCalgs.jpeg">
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        </a>
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        </div>
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<!-- END: FEATURES 1 -->
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<!-- BEGIN: CONTENT/PORTFOLIO/LATEST-WORKS-1 -->
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<div class="c-content-box c-size-md c-bg-darkblue" style="padding-bottom: 20px;">
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<div class="container">
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<div class="c-content-title-1">
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<h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Our Project</h3>
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<div class="c-line-center c-theme-bg">
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                            <div class="col-sm-3" style="padding-left:40px">
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                                <a href="https://2016.igem.org/Team:UofC_Calgary/Description">  <img class="box-img" src="https://static.igem.org/mediawiki/2016/9/9c/T--UofC_Calgary--biotarget.jpg"> </img>  </a> 
 +
                            <p style="color:#FFFFFF; text-align: center; font-size: 16px;"> Project Description </p>     
 +
                            </div>
 +
                            <div class="col-sm-3" style="padding-left:28px; padding-right:28px">
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                                  <a href="https://2016.igem.org/Team:UofC_Calgary/Experiments"><img class="box-img" src="https://static.igem.org/mediawiki/2016/4/4b/T--UofC_Calgary--chassis.jpg"> </a></img> <p style="color:#FFFFFF; text-align: center; font-size: 16px;"> Our Experiments </p>     
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                            </div>
 +
                            <div class="col-sm-3" style="padding-right:40px">   
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                                  <a href="https://2016.igem.org/Team:UofC_Calgary/Model"> <img class="box-img" src="https://static.igem.org/mediawiki/2016/4/4c/T--UofC_Calgary--device.jpg"> </img>  </a>      <p style="color:#FFFFFF; text-align: center; font-size: 16px;"> Modelling </p> 
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                            </div>
 +
                            <div class="col-sm-3" style="padding-right:40px">   
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                                  <a href="https://2016.igem.org/Team:UofC_Calgary/Human_Practices"><img class="box-img" src="https://static.igem.org/mediawiki/2016/c/c2/T--UofC_Calgary--pandp.jpg"> </img> </a>        <p style="color:#FFFFFF; text-align: center; font-size: 16px;"> Human Practices </p>
 +
                            </div>
 +
                </div>
 +
                <div class="human-practices-div-4" style="padding-top: 15px; color: #EBDEBE;"> Click to learn more about each part of our project</div>
 +
</div>
 +
</div>
 +
<!-- END: CONTENT/PORTFOLIO/LATEST-WORKS-1 -->
  
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<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">
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UPLOAD FILES
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<div class="c-content-box c-size-lg c-bg-parallax" style="background-image: url(assets/base/img/content/backgrounds/bg-29.jpg)">
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<div class="container">
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<div class="c-content-title-1">
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<h3 class="c-center c-font-white c-font-uppercase c-font-bold">Our Device</h3>
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<div class="c-line-center c-theme-bg">
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</div>
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</div>
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<div class="row c-margin-t-60">
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<div class="col-md-6">
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<div class="c-content-testimonial-2-slider" data-slider="owl" data-single-item="true" data-auto-play="6000" style="width:900px">
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<div class="c-title c-font-uppercase c-font-bold c-theme-bg">
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Microneedle Patch
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</div>
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<div class="owl-carousel owl-theme c-theme owl-single">
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<div class="item">
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<div class="c-content-testimonial-2" style="min-height: 320px;">
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<div class="c-testimonial c-font-uppercase c-font-bold">
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Transdermal Microneedle Drug Delivery Patch
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</div>
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<div class="c-author">
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<div class="c-portrait" style="background-image: url(assets/base/img/content/team/team8.jpg)">
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<div class="c-name c-font-uppercase">
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NOSHIN
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</div>
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<p class="c-position c-theme c-font-uppercase">
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3M Microneedles
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</p>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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<div class="col-md-6">
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<div class="c-content-v-center c-bg-red" style="height: 320px;width:320px;">
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<img src="https://s3.amazonaws.com/igem2016/assets/base/img/content/stock/device1.JPG" alt="" style="   
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display: block;
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<!-- BEGIN: CONTENT/MISC/ABOUT-1 -->
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<div class="container">
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<!-- Begin: Title 1 component -->
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<div class="c-content-title-1">
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<h3 class="c-font-uppercase c-font-bold">About us</h3>
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<div class="c-line-left c-theme-bg">
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</div>
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</div>
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<!-- End-->
 +
<p>
 +
The 2016 U of C Calgary iGEM team is a multidisciplinary team based out of the University of Calgary. Our team is made up of undergraduate students from all years of study, hailing from the broad backgrounds of biology, microbiology, biomedical sciences, bioinformatics, and engineering. We are based out of the O'Brien Centre Labs within the U of C's Health Science Centre.
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<!-- Begin: Title 1 component -->
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<div class="c-content-title-1">
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<h3 class="c-font-uppercase c-font-bold">Our Sponsors</h3>
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<div class="c-line-left c-theme-bg">
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</div>
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</div>
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<!-- End-->
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<div class="c-logos">
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<div class="row">
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<a href="http://www.ucalgary.ca/"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/3/35/T--UofC_Calgary--uofc.png" alt="" style="width: 180px; height:140px;"></a>
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</div>
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<div class="col-md-4 col-xs-6 c-logo c-logo-2">
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<a href="http://cumming.ucalgary.ca/bhsc/program/o%E2%80%99brien-centre-facility"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/7/74/T--UofC_Calgary--O'BrienLogo.png" alt="" style="width: 180px; height:140px;"></a>
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</div>
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<div class="col-md-4 col-xs-6 c-logo c-logo-3">
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<a href="http://schulich.ucalgary.ca/"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/c/c7/T--UofC_Calgary--uofcschulich.png" alt="" style="width: 180px; height:140px;"></a>
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</div>
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<div class="col-md-4 col-xs-6 c-logo c-logo-4">
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<a href="https://www.mindfuel.ca/?gclid=CJ_GjpW0384CFZSIfgodOW4F0A"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/1/1f/T--UofC_Calgary--mindfuel.png" alt="" style="width: 180px; height:140px;"></a>
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</div>
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<div class="col-md-4 col-xs-6 c-logo c-logo-5">
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<a href="http://www.albertatechfutures.ca/Research/BasicResearch/geekStarter.aspx"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/7/7d/T--UofC_Calgary--geekstarter.png" alt="" style="width: 180px; height:140px;"></a>
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</div>
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<div class="col-md-4 col-xs-6 c-logo c-logo-6">
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<a href="http://store.biobasic.com/"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/a/a6/T--UofC_Calgary--biobasic.png" alt="" style="width: 180px; height:140px;"></a>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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<!-- END: PAGE CONTENT -->
 
</div>
 
</div>
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+
<!-- END: PAGE CONTAINER -->
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<!-- BEGIN: LAYOUT/FOOTERS/FOOTER-5 -->
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<a name="footer"></a>
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<footer class="c-layout-footer c-layout-footer-3 c-bg-dark">
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<div class="c-prefooter">
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<div class="container">
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<div class="row">
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<div class="col-md-3">
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<div class="c-container c-first">
 +
<div class="c-content-title-1">
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<h3 class="c-font-bold c-font-white">i<span class="c-theme-font">GEM</span></h3>
 +
<div class="c-line-left hide">
 +
</div>
 +
<p class="c-text">
 +
iGEM is an international competition promoting synthetic biology as a means to solve social, economic and humanitarian problems around the globe. The iGEM Jamboree is held in Boston annually. In 2016, over 300 teams are competing against each other.
 +
</p>
 +
</div>
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<ul class="c-links">
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</ul>
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</div>
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</div>
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<div class="col-md-3">
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<div class="c-container">
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<div class="c-content-title-1">
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<h3 class="c-font-uppercase c-font-bold c-font-white">Latest Entries</h3>
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<div class="c-line-left hide">
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</div>
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</div>
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<div class="c-blog">
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<div class="c-post">
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</div>
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<div class="c-post c-last">
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<div class="c-post-img">
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<img src="https://static.igem.org/mediawiki/2016/d/dc/T--UofC_Calgary--WHMIS.jpg" alt="" class="img-responsive"/>
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</div>
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<div class="c-post-content">
 +
<h4 class="c-post-title"><a href="#">Fully Trained!</a></h4>
 +
<p class="c-text">
 +
Our entire team received a full BioSafety education from the University of Calgary! This entailed going to classes to prepare for a final quiz that tested our ability to be safe in the lab. Several of our members also had radiation training and clearance to ensure that work done with radiation was safe!
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</p>
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</div>
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<a href="#" class="btn btn-md c-btn-border-1x c-theme-btn c-btn-uppercase c-btn-square c-btn-bold c-read-more hide">Read More</a>
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<h3 class="c-font-uppercase c-font-bold c-font-white">Latest Pictures</h3>
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--UofC_Calgary--wikiinsat8-1.jpg"" class="img-responsive"/></a>
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</li>
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<li>
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/b/b8/T--UofC_Calgary--wikiinsat9.jpg" class="img-responsive"/></a>
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</li>
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<li class="c-last">
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/c/c5/T--UofC_Calgary--wikiinsat7.jpg"" class="img-responsive"/></a>
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</li>
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<ul class="c-works">
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<li class="c-first">
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/a/a5/T--UofC_Calgary--wikiinsat6.jpg" class="img-responsive" /></a>
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/9/94/T--UofC_Calgary--wikiinsat5.jpg" class="img-responsive" /></a>
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<a href="https://www.instagram.com/igemcalgary"><img src="https://static.igem.org/mediawiki/2016/f/f3/T--UofC_Calgary--wikiinsat4.jpg" class="img-responsive" /></a>
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</li>
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</ul>
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<ul class="c-works">
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<li class="c-first">
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/5/5e/T--UofC_Calgary--wikiinsat3.jpg" class="img-responsive" alt=""/></a>
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</li>
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<li>
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/2/2a/T--UofC_Calgary--wikiinsat2.jpg" class="img-responsive" alt=""/></a>
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</li>
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<li class="c-last">
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/b/b1/T--UofC_Calgary--wikiinsat1.jpg" class="img-responsive" alt=""/></a>
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</li>
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</ul>
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<a href="#" class="btn btn-md c-btn-border-1x c-theme-btn c-btn-uppercase c-btn-square c-btn-bold c-read-more hide">View More</a>
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<div class="c-container c-last">
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<h3 class="c-font-uppercase c-font-bold c-font-white">Find us</h3>
 +
<div class="c-line-left hide">
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</div>
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<p>
 +
Located in Calgary, Alberta, Canada.
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</p>
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Latest revision as of 20:38, 2 December 2016

iGEM Calgary 2016

Giant Jamboree Results

We are honoured to announce that the UofC_Calgary 2016 iGEM was incredibly successful at this year's iGEM Giant Jamboree. We received a gold medal, a nomination for Best Applied Design, and the award for Best Integrated Human Practices! We are beyond thrilled to continue the University of Calgary's tradition of excellence at iGEM, and extend our deepest gratitude to those who aided us in any form during this journey.

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Our Project

Mars and the cosmos are a tantalizingly close goal for Mankind to reach in the next century; however, there are several glaring problems that we must overcome to reach such an objective. Radiation is chief among them. On Earth, individuals annually receive 2.4 mSv of ionizing radiation (IR), which is easily tolerated by the human body. Unfortunately, without the protection of the magnetosphere, astronauts in space are exposed to high levels of IR in the range of 50 to 2000 mSv. This level of radiation causes the accumulation of deleterious double strand breaks in DNA. Despite current research into methods of IR protection, many solutions such as radiation shield coating are quite expensive, especially to transport to space. Even existing biological solutions are insufficient, such as the use of ingestible or injectable radioprotectors, which are subject to sinusuoidal pharmacokinetics. Our project is based on the administration of the naturally occuring peptide Bowman-Birk Protease Inhibitor (BBI), which has been shown to confer protection against DNA damage following radiation exposure. (Dittmann et al., 2003).

References

Dittmann, K.H., Mayer, C., and Rodemann, H.P. (2003). Radioprotection of normal tissue to improve radiotherapy: the effect of the Bowman Birk protease inhibitor. Current Medicinal Chemistry - Anticancer Agents, 3(5), 360-363.

OUR CHASSIS

To express mBBI, we had to choose a biological system that was not only capable of high expression but was robust, adaptable, and easily genetically manipulated for ease of transport and use in remote and extreme environments. After creating a list of different types of cells which could be used for the expression of our peptide, we found that Bacillus subtilis was especially suited for our patch design. This is due to many reasons. B. subtilis is able to express high amounts of recombinant protein which can be secreted into surrounding media by its own well-characterized secretion system, making it ideal for producing and delivering the correct dosage of mBBI into the user. B. subtilis is capable of forming spores—which have been found in arctic permafrost dating tens of thousands of years and yet are still viable—making it easy to store in a desiccated or frozen manner. Additionally, B. subtilis expresses high amounts of recombinase A, a protein which allows genes to be stably integrated into the chromosome.

Our strain of B. subtilis (WB800) is particularly suited for high production because it is deficient in eight extracellular proteases which could lead to the premature degradation our radioprotective peptide. Our strain will allow the peptide to be stable in the media within the patch. We conducted tests on the growth patterns of B. subtilis in the novel environment of a patch and optimized patch media to simulate an ideal growth conditions. We have also designed a genetic construct which provides inducible competency to the B. subtilis strain upon the addition of xylose, making this bacterium much easier to work with for future teams.

Design Overview






We designed our project to overcome common issues in existing technologies such as cost, portability, and durability. Our patch is portable, lightweight, safe to use, can be stored long-term, and produces and delivers peptides at a steady rate to maintain a constant concentration in the body. We worked with professionals within NASA and the CSA to integrate our solution with current space travel infrastructure, and with companies specializing in transdermal delivery to optimize the patch system. We also consulted with professionals and researchers in biomedical fields to better understand the unknown effects our delivery system would have when in use. Using this system, our project has future applications here on Earth for other occupations and individuals exposed to high levels of ionizing radiation, such as pilots, flight attendants, and miners. Our project can also be used to deliver hormones and other small molecules for use in space, serving as a "biological pharmacy."










Our Project

Project Description

Our Experiments

Modelling

Human Practices

Click to learn more about each part of our project

About us

The 2016 U of C Calgary iGEM team is a multidisciplinary team based out of the University of Calgary. Our team is made up of undergraduate students from all years of study, hailing from the broad backgrounds of biology, microbiology, biomedical sciences, bioinformatics, and engineering. We are based out of the O'Brien Centre Labs within the U of C's Health Science Centre.

Our Sponsors

iGEM

iGEM is an international competition promoting synthetic biology as a means to solve social, economic and humanitarian problems around the globe. The iGEM Jamboree is held in Boston annually. In 2016, over 300 teams are competing against each other.

Latest Entries

Fully Trained!

Our entire team received a full BioSafety education from the University of Calgary! This entailed going to classes to prepare for a final quiz that tested our ability to be safe in the lab. Several of our members also had radiation training and clearance to ensure that work done with radiation was safe!

Read More

Latest Pictures

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Find us

Located in Calgary, Alberta, Canada.

  • University of Calgary
  • igem.calgary@gmail.com

Copyright © University of Calgary 2016.