Difference between revisions of "Team:Alverno CA/Basic Parts"

 
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{{Alverno_CA}}
 
{{Alverno_CA}}
 
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<head>
 
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<title>Alverno iGEM 2016</title>
 
<title>Alverno iGEM 2016</title>
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<br>
 
<br>
 
<br>
 
<br>
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<center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
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<h2><center>Basic & Composite Parts</center></h2>
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<h1><center>Basic Parts</center></h1>
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<left><h2><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000:</a></h2></left>
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  <div class="demo" id="container">
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<p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.</p>
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        <center>
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            <ul>
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                <li style="list-style: none"><br>
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                <br>
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                </li>
  
  
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<left><h2>BBa_K2145000:</h2></left>
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                <li><img src=
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                "https://static.igem.org/mediawiki/2016/1/15/T--Alverno_CA--asiasamsquare.jpg"
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                style="height:215px;width:215px;" title=
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                "Designing parts."/>
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                </li>
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      <li><img src=
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                "https://static.igem.org/mediawiki/2016/7/78/T--Alverno_CA--samsquare.jpg"
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                style="height:215px;width:215px;" title=
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                "That's a good idea!">
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                </li>
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                <li><img src=
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                "https://static.igem.org/mediawiki/2016/a/a4/T--Alverno_CA--asiasquareboard.jpg"
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                style="height:215px;width:215px;" title=
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                "Asia is thinking about parts. ">
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                </li>
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            </ul>
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        </center>
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    </div>
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<br><br><br><br><br><br><br><br><br><br>
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<left><h3><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000(GG 95):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Forward, toward the spacer</h5>
 +
<h5>Direction of RFP: Forward, away from the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145001">BBa_K2145001(GG 96):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Reverse, away from the spacer</h5>
 +
<h5>Direction of RFP: Forward, away from the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145104">BBa_K2145104(GG 97):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Forward, toward the spacer</h5>
 +
<h5>Direction of RFP: Reverse, toward the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145105">BBa_K2145105(GG 98):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Reverse, away from the spacer</h5>
 +
<h5>Direction of RFP: Reverse, toward the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145124">BBa_K2145124(GG 99):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Forward, away from the spacer</h5>
 +
<h5>Direction of RFP: Reverse, away from the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145107">BBa_K2145107(GG 100):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Reverse, toward the spacer</h5>
 +
<h5>Direction of RFP: Forward, toward the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145126">BBa_K2145126(GG 101):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Forward, away from the spacer</h5>
 +
<h5>Direction of RFP: Reverse, away from the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145127">BBa_K2145127(GG 102):</a></h3></left>
 +
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 +
<h5>Direction of GFP: Reverse, toward the spacer</h5>
 +
<h5>Direction of RFP: Reverse, away from the spacer</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145100">BBa_K2145100(GG 105):</a></h3></left>
 +
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5>
 +
<h5>Direction of GFP: Forward, toward the clamps</h5>
 +
<h5>Direction of RFP: Forward, away from the clamps</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145101">BBa_K2145101(GG 106):</a></h3></left>
 +
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5>
 +
<h5>Direction of GFP: Reverse, away from the clamps</h5>
 +
<h5>Direction of RFP: Forward, away from the clamps</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145102">BBa_K2145102(GG 107):</a></h3></left>
 +
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5>
 +
<h5>Direction of GFP: Forward, toward the clamps</h5>
 +
<h5>Direction of RFP: Reverse, toward the clamps</h5>
 +
 
 +
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145103">BBa_K2145103(GG 108):</a></h3></left>
 +
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5>
 +
<h5>Direction of GFP: Reverse, away from the clamps</h5>
 +
<h5>Direction of RFP: Reverse, toward the clamps</h5>
 +
 
  
 
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Latest revision as of 21:20, 2 December 2016

Alverno iGEM 2016

Basic & Composite Parts













BBa_K2145000(GG 95):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, toward the spacer
Direction of RFP: Forward, away from the spacer

BBa_K2145001(GG 96):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, away from the spacer
Direction of RFP: Forward, away from the spacer

BBa_K2145104(GG 97):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, toward the spacer
Direction of RFP: Reverse, toward the spacer

BBa_K2145105(GG 98):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, away from the spacer
Direction of RFP: Reverse, toward the spacer

BBa_K2145124(GG 99):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, away from the spacer
Direction of RFP: Reverse, away from the spacer

BBa_K2145107(GG 100):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, toward the spacer
Direction of RFP: Forward, toward the spacer

BBa_K2145126(GG 101):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, away from the spacer
Direction of RFP: Reverse, away from the spacer

BBa_K2145127(GG 102):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, toward the spacer
Direction of RFP: Reverse, away from the spacer

BBa_K2145100(GG 105):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Forward, toward the clamps
Direction of RFP: Forward, away from the clamps

BBa_K2145101(GG 106):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Reverse, away from the clamps
Direction of RFP: Forward, away from the clamps

BBa_K2145102(GG 107):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Forward, toward the clamps
Direction of RFP: Reverse, toward the clamps

BBa_K2145103(GG 108):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Reverse, away from the clamps
Direction of RFP: Reverse, toward the clamps