Difference between revisions of "Team:Alverno CA/Basic Parts"
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<h2><center>Basic & Composite Parts</center></h2> | <h2><center>Basic & Composite Parts</center></h2> | ||
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<li><img src= | <li><img src= | ||
| − | "https://static.igem.org/mediawiki/2016/ | + | "https://static.igem.org/mediawiki/2016/1/15/T--Alverno_CA--asiasamsquare.jpg" |
style="height:215px;width:215px;" title= | style="height:215px;width:215px;" title= | ||
"Designing parts."/> | "Designing parts."/> | ||
</li> | </li> | ||
<li><img src= | <li><img src= | ||
| − | " | + | "https://static.igem.org/mediawiki/2016/7/78/T--Alverno_CA--samsquare.jpg" |
style="height:215px;width:215px;" title= | style="height:215px;width:215px;" title= | ||
| − | + | "That's a good idea!"> | |
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<li><img src= | <li><img src= | ||
| − | " | + | "https://static.igem.org/mediawiki/2016/a/a4/T--Alverno_CA--asiasquareboard.jpg" |
style="height:215px;width:215px;" title= | style="height:215px;width:215px;" title= | ||
| − | + | "Asia is thinking about parts. "> | |
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<left><h3><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000(GG 95):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000(GG 95):</a></h3></left> | ||
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Forward, toward the spacer</h5> | ||
| + | <h5>Direction of RFP: Forward, away from the spacer</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145001">BBa_K2145001(GG 96):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145001">BBa_K2145001(GG 96):</a></h3></left> | ||
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Reverse, away from the spacer</h5> | ||
| + | <h5>Direction of RFP: Forward, away from the spacer</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145104">BBa_K2145104(GG 97):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145104">BBa_K2145104(GG 97):</a></h3></left> | ||
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Forward, toward the spacer</h5> | ||
| + | <h5>Direction of RFP: Reverse, toward the spacer</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145105">BBa_K2145105(GG 98):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145105">BBa_K2145105(GG 98):</a></h3></left> | ||
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Reverse, away from the spacer</h5> | ||
| + | <h5>Direction of RFP: Reverse, toward the spacer</h5> | ||
| − | <left><h3><a href="http://parts.igem.org/Part: | + | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145124">BBa_K2145124(GG 99):</a></h3></left> |
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Forward, away from the spacer</h5> | ||
| + | <h5>Direction of RFP: Reverse, away from the spacer</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145107">BBa_K2145107(GG 100):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145107">BBa_K2145107(GG 100):</a></h3></left> | ||
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Reverse, toward the spacer</h5> | ||
| + | <h5>Direction of RFP: Forward, toward the spacer</h5> | ||
| − | <left><h3><a href="http://parts.igem.org/Part: | + | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145126">BBa_K2145126(GG 101):</a></h3></left> |
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Forward, away from the spacer</h5> | ||
| + | <h5>Direction of RFP: Reverse, away from the spacer</h5> | ||
| − | <left><h3><a href="http://parts.igem.org/Part: | + | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145127">BBa_K2145127(GG 102):</a></h3></left> |
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | <h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5> | ||
| + | <h5>Direction of GFP: Reverse, toward the spacer</h5> | ||
| + | <h5>Direction of RFP: Reverse, away from the spacer</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145100">BBa_K2145100(GG 105):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145100">BBa_K2145100(GG 105):</a></h3></left> | ||
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | <h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | ||
| + | <h5>Direction of GFP: Forward, toward the clamps</h5> | ||
| + | <h5>Direction of RFP: Forward, away from the clamps</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145101">BBa_K2145101(GG 106):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145101">BBa_K2145101(GG 106):</a></h3></left> | ||
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | <h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | ||
| + | <h5>Direction of GFP: Reverse, away from the clamps</h5> | ||
| + | <h5>Direction of RFP: Forward, away from the clamps</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145102">BBa_K2145102(GG 107):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145102">BBa_K2145102(GG 107):</a></h3></left> | ||
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | <h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | ||
| + | <h5>Direction of GFP: Forward, toward the clamps</h5> | ||
| + | <h5>Direction of RFP: Reverse, toward the clamps</h5> | ||
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145103">BBa_K2145103(GG 108):</a></h3></left> | <left><h3><a href="http://parts.igem.org/Part:BBa_K2145103">BBa_K2145103(GG 108):</a></h3></left> | ||
<h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | <h5>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</h5> | ||
| − | + | <h5>Direction of GFP: Reverse, away from the clamps</h5> | |
| + | <h5>Direction of RFP: Reverse, toward the clamps</h5> | ||
Latest revision as of 21:20, 2 December 2016
