Difference between revisions of "Team:Alverno CA/Basic Parts"

 
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<br>
 
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<center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
 
 
<h2><center>Basic & Composite Parts</center></h2>
 
<h2><center>Basic & Composite Parts</center></h2>
  
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                 <li><img src=
 
                 <li><img src=
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                 "https://static.igem.org/mediawiki/2016/f/f8/T--Alverno_CA--Asia_n_Sam_parts.jpg"
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                 "https://static.igem.org/mediawiki/2016/1/15/T--Alverno_CA--asiasamsquare.jpg"
 
                 style="height:215px;width:215px;" title=
 
                 style="height:215px;width:215px;" title=
 
                 "Designing parts."/>
 
                 "Designing parts."/>
 
                 </li>
 
                 </li>
 
       <li><img src=
 
       <li><img src=
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                 "https://static.igem.org/mediawiki/2016/f/ff/T--Alverno_CA--Sam.jpg"
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                 "https://static.igem.org/mediawiki/2016/7/78/T--Alverno_CA--samsquare.jpg"
 
                 style="height:215px;width:215px;" title=
 
                 style="height:215px;width:215px;" title=
 
                 "That's a good idea!">
 
                 "That's a good idea!">
 
                 </li>
 
                 </li>
 
                 <li><img src=
 
                 <li><img src=
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                 "https://static.igem.org/mediawiki/2016/a/aa/T--Alverno_CA--Asia%26Parts.jpg"
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                 "https://static.igem.org/mediawiki/2016/a/a4/T--Alverno_CA--asiasquareboard.jpg"
 
                 style="height:215px;width:215px;" title=
 
                 style="height:215px;width:215px;" title=
 
                 "Asia is thinking about parts. ">
 
                 "Asia is thinking about parts. ">
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<h5>Direction of RFP: Reverse, toward the spacer</h5>
 
<h5>Direction of RFP: Reverse, toward the spacer</h5>
  
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<left><h3><a href="http://parts.igem.org/Part:BBa_K2145104">BBa_K2145124(GG 99):</a></h3></left>
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<left><h3><a href="http://parts.igem.org/Part:BBa_K2145124">BBa_K2145124(GG 99):</a></h3></left>
 
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 
<h5>Direction of GFP: Forward, away from the spacer</h5>
 
<h5>Direction of GFP: Forward, away from the spacer</h5>
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<h5>Direction of RFP: Forward, toward the spacer</h5>
 
<h5>Direction of RFP: Forward, toward the spacer</h5>
  
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<left><h3><a href="http://parts.igem.org/Part:BBa_K2145104">BBa_K2145126(GG 101):</a></h3></left>
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<left><h3><a href="http://parts.igem.org/Part:BBa_K2145126">BBa_K2145126(GG 101):</a></h3></left>
 
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 
<h5>Direction of GFP: Forward, away from the spacer</h5>
 
<h5>Direction of GFP: Forward, away from the spacer</h5>
 
<h5>Direction of RFP: Reverse, away from the spacer</h5>
 
<h5>Direction of RFP: Reverse, away from the spacer</h5>
  
−
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145104">BBa_K2145127(GG 102):</a></h3></left>
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<left><h3><a href="http://parts.igem.org/Part:BBa_K2145127">BBa_K2145127(GG 102):</a></h3></left>
 
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 
<h5>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</h5>
 
<h5>Direction of GFP: Reverse, toward the spacer</h5>
 
<h5>Direction of GFP: Reverse, toward the spacer</h5>

Latest revision as of 21:20, 2 December 2016

Alverno iGEM 2016

Basic & Composite Parts













BBa_K2145000(GG 95):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, toward the spacer
Direction of RFP: Forward, away from the spacer

BBa_K2145001(GG 96):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, away from the spacer
Direction of RFP: Forward, away from the spacer

BBa_K2145104(GG 97):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, toward the spacer
Direction of RFP: Reverse, toward the spacer

BBa_K2145105(GG 98):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, away from the spacer
Direction of RFP: Reverse, toward the spacer

BBa_K2145124(GG 99):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, away from the spacer
Direction of RFP: Reverse, away from the spacer

BBa_K2145107(GG 100):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, toward the spacer
Direction of RFP: Forward, toward the spacer

BBa_K2145126(GG 101):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Forward, away from the spacer
Direction of RFP: Reverse, away from the spacer

BBa_K2145127(GG 102):
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
Direction of GFP: Reverse, toward the spacer
Direction of RFP: Reverse, away from the spacer

BBa_K2145100(GG 105):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Forward, toward the clamps
Direction of RFP: Forward, away from the clamps

BBa_K2145101(GG 106):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Reverse, away from the clamps
Direction of RFP: Forward, away from the clamps

BBa_K2145102(GG 107):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Forward, toward the clamps
Direction of RFP: Reverse, toward the clamps

BBa_K2145103(GG 108):
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
Direction of GFP: Reverse, away from the clamps
Direction of RFP: Reverse, toward the clamps