Difference between revisions of "Team:USP UNIFESP-Brazil/Notebook"

 
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<p class=black>The lab notebook is the window to our soul. Here we describe all those weeks of experiments, with proper attributions to the wetlab iGEMers! We have separated it in two parts. A (more) organized notebook with the experiments curated by topic and the full lab notebook, with everything we documented from the wet lab!</p>
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                <img style="max-width:107%!important;margin-left:-30px;" src="https://static.igem.org/mediawiki/2016/c/c5/T--USP_UNIFESP-Brazil--attributions_students.png">
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<!--O texto do parágrafo viria aqui.-->
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<h2 class=black><center>Full notebook </center></h2>
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        <p class=black> Here you can find our full notebook, with troubleshooting, annotations, preparation of reagents, optimization of processes and, of course, experiments. If you are brave enough, you can download right below or (depending on your browser) read the text online! You can also find a compilation of all the protocols we used <u><a href=https://static.igem.org/mediawiki/2016/3/30/T--USP_UNIFESP-Brazil--Protocols2.pdf>right here!<a></u></p>
  
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<p><strong> TIP: Use the PDF bookmarks to navigate among different weeks of experiments!</strong></p>
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<u><a href=https://static.igem.org/mediawiki/2016/0/00/T--USP_UNIFESP-Brazil--labnotebook_fullnotebook_withoutlastweek.pdf>Click here to download it<a></u>
  
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<object data="https://static.igem.org/mediawiki/2016/0/00/T--USP_UNIFESP-Brazil--labnotebook_fullnotebook_withoutlastweek.pdf" type="application/pdf" width="700px" height="700px">
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    <embed src="https://static.igem.org/mediawiki/2016/0/00/T--USP_UNIFESP-Brazil--labnotebook_fullnotebook_withoutlastweek.pdf">
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        This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2016/0/00/T--USP_UNIFESP-Brazil--labnotebook_fullnotebook_withoutlastweek.pdf">Download PDF</a>.</p>
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              <h2 class=black><center> Divided notebook </center</h2>
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<p class=black> If you get lost by browsing through our complete notebook <strike>(we get lost too!)</strike>, we are providing our divided version by research fronts that were addressed in our project!</p>
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PIs and instructors
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<u><a href=https://static.igem.org/mediawiki/2016/9/9a/T--USP_UNIFESP-Brazil--Gathering_Expression.pdf>Gathering of expression vector units here!<a></u>
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<p class=black><strong>Primary Principal Investigator (Primary PI) - Professor João Carlos Monteiro de Carvalho, Ph.D. </strong> Our team really appreciate Professor João’s contribution to the team with his expertise about algae cultivation. He also, in a position of authority, assisted with official aspects in the University. We thank him for believing in us and providing laboratory space, some reagents and equipment.</p>
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<object data="https://static.igem.org/mediawiki/2016/9/9a/T--USP_UNIFESP-Brazil--Gathering_Expression.pdf" type="application/pdf" width="700px" height="700px">
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    <embed src="https://static.igem.org/mediawiki/2016/9/9a/T--USP_UNIFESP-Brazil--Gathering_Expression.pdf">
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        This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2016/9/9a/T--USP_UNIFESP-Brazil--Gathering_Expression.pdf">Download PDF</a>.</p>
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<p class=black><strong>Secondary Principal Investigator (Secondary PI) - Livia Seno Ferreira Camargo, Ph.D. </strong>Our team would like to thank Livia who contributed with her expertise in algae molecular biology and cultivation. She provided great help with team registration, fund raising, giving support throughout the season and helping on the contact with IGEM for updates, and companies for laboratory supplies and donations.</p>
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<u><a href=https://static.igem.org/mediawiki/2016/3/36/T--USP_UNIFESP-Brazil--General_assembling.pdf>Download General sequences assembly here!<a></u>
  
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<p  class=black><strong>Instructor - João Vitor Dutra Molino. </strong>Our team would like to say thank you to João, who contributed with his expertise in algae molecular biology, cultivation and protein purification. He motivated the team by planning experiments, gathering team members and giving a day to day support. He also worked hard on tasks as lab duties, fund raising, laboratory training and hardware development together.</p>
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<object data="https://static.igem.org/mediawiki/2016/3/36/T--USP_UNIFESP-Brazil--General_assembling.pdf" type="application/pdf" width="700px" height="700px">
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    <embed src="https://static.igem.org/mediawiki/2016/3/36/T--USP_UNIFESP-Brazil--General_assembling.pdf">
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        This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2016/3/36/T--USP_UNIFESP-Brazil--General_assembling.pdf">Download PDF</a>.</p>
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<p class=black><strong>Instructor - Thiago Mosqueiro, Ph.D. </strong>We would like to thank Thiago who contributed with his expertise in data analysis and scientific programming by helping in the analysis of the screening experiment. He also helped us write pieces of code to analyse our fluorescence data.</p>
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<u><a href=https://static.igem.org/mediawiki/2016/2/2c/T--USP_UNIFESP-Brazil--USER.pdf>Curious about USER multimerization? here!<a></u>
  
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    <embed src="https://static.igem.org/mediawiki/2016/2/2c/T--USP_UNIFESP-Brazil--USER.pdf">
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        This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2016/2/2c/T--USP_UNIFESP-Brazil--USER.pdf">Download PDF</a>.</p>
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<u><a href=https://static.igem.org/mediawiki/2016/5/5a/T--USP_UNIFESP-Brazil--Expression_Lysos.pdf>Wanna view Lysostaphin expression?<a></u>
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        This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2016/5/5a/T--USP_UNIFESP-Brazil--Expression_Lysos.pdf">Download PDF</a>.</p>
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Sponsors
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<u><a href=https://static.igem.org/mediawiki/2016/9/9b/T--USP_UNIFESP-Brazil--Assembly_by_restriction_enzyme.pdf>Assembly by restriction enzyme of Lh Masp1 Type2<a></u>
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Special Thanks
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        This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2016/9/9b/T--USP_UNIFESP-Brazil--Assembly_by_restriction_enzyme.pdf">Download PDF</a>.</p>
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<p class=black>USP_UNIFESP-BRAZIL team really appreciate the support given by Professors, who allowed us to use their laboratory space, reagents and equipment, and by their knowledge contribution; Company's employees, who were reached easily and gave their best to make the donation process faster, and Institutions and Companies that helped us by publishing our ideas and project.</p>
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<li class=black>Lukasz Kowalik (Merck KGaA)</li>
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<li class=black>Marlene Lunardi (Thermo Fisher Scientific)</li>
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<li class=black>Otto Heringer and Danilo Zampronio (Lotan)</li>
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<li class=black>Research Ideas and Outcomes (Journal RIO)</li>
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<li class=black>S&atilde;o Paulo Research Foundation (FAPESP)</li>
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<p class=black>University of S&atilde;o Paulo/Pharmacy School:</p>
 
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<li class=black>Professor Ricardo Pinheiro de Souza Oliveira, Ph.D. </li>
 
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<li class=black>Professor Gisele Monteiro, Ph.D. </li>
 
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<li class=black>Professor Mario Hiroyuki Hirata, Ph.D.</li>
 
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<p class=black>University of S&atilde;o Paulo/Biomedical Sciences Institute:</p>
 
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<li class=black>Professor Luiziana Ferreira da Silva, Ph.D. </li>
 
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<li class=black>Professor Jos&eacute; Greg&oacute;rio Cabrera Gomez, Ph.D.</li>
 
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<li class=black>Professor Marcio Bertacine Dias, Ph.D.</li>
 
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<p class=black>University of S&atilde;o Paulo/Institute of Chemistry</p>
 
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<li class=black>Professor Alexandre Bruni-Cardoso, Ph.D.</li>
 
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<li class=black>William Cenens, Ph.D.</li>
 
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<p class=black>University of S&atilde;o Paulo/Polytechnic School:</p>
 
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<p class=black>University of California, San Diego/Biological Sciences Division:</p>
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<p class=black>S&atilde;o Paulo State University, Assis/Dep. of Biotechnology:</p>
 
  
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<li class=black>Professor Edislane B. de Souza, Ph.D.</li>
 
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<li class=black>Professor Dario A. Palmieri, Ph.D.</li>
 
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<li class=black>Professor Pedro O. Neto, Ph.D.</li>
 
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<p class=black>S&atilde;o Paulo State University, Presidente Prudente/Dep. of Chemistry and Biochemistry:</p>
 
  
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<li class=black>Professor Ana Maria Pires, Ph.D.</li>
 
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<p class=black>Brazilian Agricultural Research Corporation (EMBRAPA - Genetic Resources &amp; Biotechnology)</p>
 
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<li class=black>Elibio Leopoldo Rech Filho, Ph.D.</li>
 
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<p class=black><strong>What if we could spread our project idea in media and press?</strong></p>
 
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<p class=black>That was one of the first questions that our team asked, and the answer was: yes! The first report was launched by the brazilian website TechMundo (World Technology) in May 2016 and named &ldquo;Algaranha: Brazilian create webs from algae to heal burns&rdquo;. The report highlighted the project objectives and explained the iGEM competition.</p>
 
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<p class=black>The paper &ldquo;Chimeric spider silk production in microalgae: a modular bionanomaterial&rdquo; was released on June 23rd 2016 by the innovative, open access journal RIO (Research Ideas and Outcomes). This paper explains the project idea, emphasizing the possibility of exploring the modular characteristic of spider silk proteins and describes the methods to achieve it.</p>
 
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<p class=black>The report entitled &ldquo;Ready to Compete&rdquo; was publicly released in September 2016 by &lsquo;Pesquisa FAPESP&rsquo;, a magazine published by S&atilde;o Paulo Research Foundation with a current circulation of 44,000. The report describes the iGEM competition, our project and the lack of funds that was faced by our team. It was also discussed the devices created and made by our team, as the microcentrifuge, made from MDF panel, and a DNA electrophoresis buffer tank, made of acrylic. This report was published in print edition and online, shared in academia through a workshop, reaching brazilian researchers and students from the Univesity.</p>
 
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<p class=black>All these press coverage have significantly contributed to the crowdfunding (you can read more in the crowdfunding link) and increased group visibility. The reports were also shared in our social media, and the received positive comments encouraged us to keep our focus on the project and face the obstacles.</p>
 
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<p class=black><strong>References:</strong></p>
 
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<p class=black>Chimeric spider silk production in microalgae: a modular bionanomaterial. Available in: &lt;<a>http://rio.pensoft.net/articles.php?id=9342</a>&gt;. Accessed: Oct 9th 2016.</p>
 
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<p class=black>Algaranha: Brazilian create webs from algae to heal burns. Available in: &lt;<a>http://www.tecmundo.com.br/ciencia/104362-algaranha-brasileiros-criam-teias-partir-algas-curar-queimaduras.htm</a>&gt;. Accessed: Oct 9th 2016.</p>
 
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<p class=black>Ready to Compete. Available in: &lt;<a>http://revistapesquisa.fapesp.br/2016/09/23/prontos-para-competir/</a>&gt;. Accessed: Oct 9th 2016.</p>
 
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Latest revision as of 01:48, 3 December 2016

AlgAranha Team USP_UNIFESP-Brazil

iGEM 2016

Notebook

The lab notebook is the window to our soul. Here we describe all those weeks of experiments, with proper attributions to the wetlab iGEMers! We have separated it in two parts. A (more) organized notebook with the experiments curated by topic and the full lab notebook, with everything we documented from the wet lab!

Full notebook

Here you can find our full notebook, with troubleshooting, annotations, preparation of reagents, optimization of processes and, of course, experiments. If you are brave enough, you can download right below or (depending on your browser) read the text online! You can also find a compilation of all the protocols we used right here!

TIP: Use the PDF bookmarks to navigate among different weeks of experiments!

Click here to download it This browser does not support PDFs. Please download the PDF to view it: Download PDF.

Divided notebook

If you get lost by browsing through our complete notebook (we get lost too!), we are providing our divided version by research fronts that were addressed in our project!

Gathering of expression vector units here! This browser does not support PDFs. Please download the PDF to view it: Download PDF.

 Download General sequences assembly here! This browser does not support PDFs. Please download the PDF to view it: Download PDF.

 Curious about USER multimerization? here! This browser does not support PDFs. Please download the PDF to view it: Download PDF.

 Wanna view Lysostaphin expression? This browser does not support PDFs. Please download the PDF to view it: Download PDF.

 Assembly by restriction enzyme of Lh Masp1 Type2 This browser does not support PDFs. Please download the PDF to view it: Download PDF.