Difference between revisions of "Team:NYU-AD"
Ekdlsjubilee (Talk | contribs) |
|||
| (48 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
<html lang="en"> | <html lang="en"> | ||
<head> | <head> | ||
| Line 11: | Line 10: | ||
<!-- the above link is for local work --> | <!-- the above link is for local work --> | ||
| − | <link href="https://2016.igem.org/Team:NYU-AD/libraries/ | + | <link href="https://2016.igem.org/Team:NYU-AD/libraries/theCSSFilev9?action=raw&ctype=text/css" rel="stylesheet"> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<script type="text/javascript" src="https://2016.igem.org/Team:NYU-AD/libraries/jquery-1_12_4_min_js?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="https://2016.igem.org/Team:NYU-AD/libraries/jquery-1_12_4_min_js?action=raw&ctype=text/javascript"></script> | ||
<script type="text/javascript" src="https://2016.igem.org/Team:NYU-AD/libraries/Bootstrap-js?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="https://2016.igem.org/Team:NYU-AD/libraries/Bootstrap-js?action=raw&ctype=text/javascript"></script> | ||
| Line 75: | Line 70: | ||
#top_title, #sideMenu { | #top_title, #sideMenu { | ||
display: none; | display: none; | ||
| + | } | ||
| + | a { | ||
| + | color: #000000; | ||
| + | text-decoration: none; | ||
} | } | ||
a:visited { | a:visited { | ||
color: #000000; | color: #000000; | ||
| + | } | ||
| + | |||
| + | #big-container a:visited { | ||
| + | color: #002bb8; | ||
} | } | ||
| Line 84: | Line 87: | ||
/* | /* | ||
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////*/ | ////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////*/ | ||
| − | + | /*///////////////////////the below setting will be used for each page except the HOME PAGE///////////////////////////////////////////////////////////////////////////////*/ | |
</style> | </style> | ||
| − | + | <!-- ////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// --> | |
| − | + | ||
</head> | </head> | ||
| Line 103: | Line 105: | ||
<i class="fa fa-bars"></i> | <i class="fa fa-bars"></i> | ||
</button> | </button> | ||
| − | + | <a href="https://2016.igem.org/Team:NYU-AD"> | |
<img id="nav-logo" src="https://static.igem.org/mediawiki/2016/9/9c/T--NYU-AD--NYUAD_logo.jpg" width="250px" height="80px"/> | <img id="nav-logo" src="https://static.igem.org/mediawiki/2016/9/9c/T--NYU-AD--NYUAD_logo.jpg" width="250px" height="80px"/> | ||
| − | + | </a> | |
</div> | </div> | ||
<div class="collapse navbar-collapse" id="navbar-collapse-1"> | <div class="collapse navbar-collapse" id="navbar-collapse-1"> | ||
| Line 115: | Line 117: | ||
<li><a href="https://2016.igem.org/Team:NYU-AD/Design">Design</a></li> | <li><a href="https://2016.igem.org/Team:NYU-AD/Design">Design</a></li> | ||
<li><a href="https://2016.igem.org/Team:NYU-AD/Experiments">Experiments</a></li> | <li><a href="https://2016.igem.org/Team:NYU-AD/Experiments">Experiments</a></li> | ||
| − | + | ||
| − | + | ||
<li><a href="https://2016.igem.org/Team:NYU-AD/Results">Results</a></li> | <li><a href="https://2016.igem.org/Team:NYU-AD/Results">Results</a></li> | ||
<li><a href="https://2016.igem.org/Team:NYU-AD/Notebook">Notebook</a></li> | <li><a href="https://2016.igem.org/Team:NYU-AD/Notebook">Notebook</a></li> | ||
| Line 132: | Line 134: | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a class="dropdown-toggle" data-toggle="dropdown" href="#"> | + | <a href="https://2016.igem.org/Team:NYU-AD/Parts">PARTS</a> |
| + | |||
| + | |||
| + | |||
| + | |||
| + | </li> | ||
| + | <li class="dropdown"> | ||
| + | <a class="dropdown-toggle" data-toggle="dropdown" href="#">HUMAN PRACTICES<span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a href="https://2016.igem.org/Team:NYU-AD/ | + | <li><a href="https://2016.igem.org/Team:NYU-AD/Human_Practices">Human Practices</a></li> |
| − | <li><a href="https://2016.igem.org/Team:NYU-AD/ | + | <li><a href="https://2016.igem.org/Team:NYU-AD/HP/Silver">Silver Criterion</a></li> |
| − | <li><a href="https://2016.igem.org/Team:NYU-AD/ | + | <li><a href="https://2016.igem.org/Team:NYU-AD/HP/Gold">Gold Criterion</a></li> |
| − | <li><a href="https://2016.igem.org/Team:NYU-AD/ | + | <li><a href="https://2016.igem.org/Team:NYU-AD/Integrated_Practices">Integrated Practices</a></li> |
| − | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | + | ||
<li><a href="https://2016.igem.org/Team:NYU-AD/Safety">SAFETY</a></li> | <li><a href="https://2016.igem.org/Team:NYU-AD/Safety">SAFETY</a></li> | ||
| Line 156: | Line 165: | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 186: | Line 174: | ||
</header> | </header> | ||
| − | + | ||
| + | <main id="main" class="site-main"> | ||
<section class="section section-center section-cta section-top"> | <section class="section section-center section-cta section-top"> | ||
<div class="container"> | <div class="container"> | ||
| − | + | <div class="row"> | |
| − | + | <div class="col-md-12"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2016/1/13/T--NYU-AD--igem-logo.png" width="500" id="home-page-logo" /><br/><br/> | |
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/1/1e/T--NYU-AD--logo-headingv2.png" width="500" id="home-page-logo-heading" /><br/><br/> |
| − | + | ||
<!-- <p style="font-size: 42px; color: #1aa89d; text-align: center; font-family: 'Roboto Slab', Georgia, "Times New Roman", Times, serif; font-weight: 900;"> | <!-- <p style="font-size: 42px; color: #1aa89d; text-align: center; font-family: 'Roboto Slab', Georgia, "Times New Roman", Times, serif; font-weight: 900;"> | ||
| Line 200: | Line 189: | ||
</p> --> | </p> --> | ||
| − | <a href="#main-intro" id="scroll-down"><img src="https://static.igem.org/mediawiki/2016/ | + | <a href="#main-intro" id="scroll-down"><img src="https://static.igem.org/mediawiki/2016/c/c7/T--NYU-AD--arrow.png" width="50px" height="50px"/></a> |
| − | + | </div> | |
| − | + | </div> | |
</div> | </div> | ||
</section> | </section> | ||
| − | + | <section id="main-intro" class="section section-center section-cta"> | |
<div class="container"> | <div class="container"> | ||
| − | + | <div class="row"> | |
| − | + | <div class="col-md-12"> | |
| − | + | ||
| − | + | <h1 id="project-abstract-heading">Project Abstract</h1> | |
| − | + | <p id="abstract-para">In many developing countries, people depend on reasonably priced and conveniently available street food. However, lack of action taken by governments to regulate street food vendors has led to the prevalence of severe street food-related illnesses. One of the primary microbial contaminants in street food is <i>E. coli</i> O157:H7, which acts by secreting the Shiga-like toxin (SLT). Currently, there is no detection method for SLT outside of a lab setting, thus putting the consumers of foods at risk. Our project aims to develop a device that would be used by street vendors and restaurant owners to verify the safety of their products. Through our device, we exploit the binding of Gb3 to subunit B of the Shiga toxin, and compare the migration pattern of the bound Gb3-subunit B complex to a non bound subunit B. A shift in the migration pattern on a PAGE gel will occur when Gb3 is bound, indicating the presence of the toxin in the food sample. If no shift occurs in the SLT migration pattern, this implies the absence of the toxin within the sample, and reflects the safety status of the food.</p> | |
| − | + | ||
| − | + | <br> | |
| − | + | <br> | |
| − | + | </div> | |
| − | + | ||
| + | </div> | ||
</div> | </div> | ||
</section> | </section> | ||
| Line 222: | Line 212: | ||
<section id="features" class="section section-center section-hilite section-features"> | <section id="features" class="section section-center section-hilite section-features"> | ||
<div class="container"> | <div class="container"> | ||
| − | <h2 class="section-title"><span>Interested ? | + | <h2 class="section-title"><span>Interested? Find out more about our...</span></h2> |
| + | <br> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-md-4 col-sm-6"> | + | <div class="col-md-4 col-sm-6 icon000"> |
| − | <div class="icon-wrap"><img src=" | + | <a href="https://2016.igem.org/Team:NYU-AD/Description"><div class="icon-wrap"><img src="https://static.igem.org/mediawiki/2016/e/e6/T--NYU-AD--iconno1.png" alt=""></div> |
| − | <h4> | + | <h4>Project Description</h4></a> |
| − | + | ||
</div> | </div> | ||
| − | <div class="col-md-4 col-sm-6"> | + | <div class="col-md-4 col-sm-6 icon000"> |
| − | <div class="icon-wrap"><img src=" | + | <a href="https://2016.igem.org/Team:NYU-AD/Human_Practices"><div class="icon-wrap"><img src="https://static.igem.org/mediawiki/2016/6/60/T--NYU-AD--iconno2.png" alt=""></div> |
| − | <h4> | + | <h4>Human Practices</h4></a> |
| − | + | ||
</div> | </div> | ||
| − | <div class="col-md-4 col-sm-6"> | + | <div class="col-md-4 col-sm-6 icon000"> |
| − | + | <a href="https://2016.igem.org/Team:NYU-AD/Team"> <div class="icon-wrap"><img src="https://static.igem.org/mediawiki/2016/8/88/T--NYU-AD--iconno3.png" alt=""></div> | |
| − | <h4> | + | <h4>Team</h4></a> |
| − | + | ||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| + | <br><br><br><br> | ||
</div> | </div> | ||
</section> | </section> | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</body> | </body> | ||
</html> | </html> | ||
| + | {{:Team:NYU-AD/footer}} | ||
Latest revision as of 03:36, 3 December 2016
Project Abstract
In many developing countries, people depend on reasonably priced and conveniently available street food. However, lack of action taken by governments to regulate street food vendors has led to the prevalence of severe street food-related illnesses. One of the primary microbial contaminants in street food is E. coli O157:H7, which acts by secreting the Shiga-like toxin (SLT). Currently, there is no detection method for SLT outside of a lab setting, thus putting the consumers of foods at risk. Our project aims to develop a device that would be used by street vendors and restaurant owners to verify the safety of their products. Through our device, we exploit the binding of Gb3 to subunit B of the Shiga toxin, and compare the migration pattern of the bound Gb3-subunit B complex to a non bound subunit B. A shift in the migration pattern on a PAGE gel will occur when Gb3 is bound, indicating the presence of the toxin in the food sample. If no shift occurs in the SLT migration pattern, this implies the absence of the toxin within the sample, and reflects the safety status of the food.
