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| + | {{UPMC-Paris}} |
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− | <style> | + | <div class="column full_size"> |
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− | /********************************* DEFAULT WIKI SETTINGS ********************************/
| |
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− | #sideMenu, #top_title {display:none;}
| + | <p>Describe the experiments, research and protocols you used in your iGEM project.</p> |
− | #content { padding:0px; width:1000px; margin-top:-7px; margin-left:0px;}
| + | |
− | body {background-color:white; }
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− | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
| + | |
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− | /********************************* MENU ********************************/ | + | </div> |
− | /* Wrapper for the menu */
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− | .menu_wrapper {
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− | width:100%;
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− | height:100vh;
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− | position:fixed;
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− | padding:0px;
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− | float:left;
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− | background-color:#f2f2f2;
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− | text-align:left;
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− | }
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− | /* styling for the menu items */
| + | <div class="column half_size"> |
− | .menu_item {
| + | <h5>What should this page contain?</h5> |
− | width:100%;
| + | <ul> |
− | margin:-2px 0px 0px -20px;
| + | <li> Protocols </li> |
− | padding: 10px 10px;
| + | <li> Experiments </li> |
− | border-bottom: 1px solid #d3d3d3;
| + | <li>Documentation of the development of your project </li> |
− | font-weight:bold;
| + | </ul> |
− | color:#000000;
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− | cursor: pointer;
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− | }
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− | | + | |
− | /* when hovering on a menu item */
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− | .menu_item:hover {
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− | color:#000000;
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− | background-color: #72c9b6;
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− | }
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− | | + | |
− | /* decoration icon for the menu buttons*/
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− | .icon {
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− | float:right;
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− | font-size:16px;
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− | font-weight:bold;
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− | }
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− |
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− | /* this is the icon for when the content is collapsed */
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− | .plus::before {
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− | content: "+";
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− | }
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− | /* this is the icon for when the content is expanded */
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− | .less::before {
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− | content: "–";
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− | }
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− | | + | |
− | /* styling for the ul that creates the buttons */
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− | ul.menu_items {
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− | width:100%;
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− | margin-left:0px;
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− | list-style: none;
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− | }
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− | | + | |
− | /* styling for the li that are the menu items */
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− | .menu_items li {
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− | width:90%;
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− | margin-top:-2px;
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− | padding: 15px 0px 15px 15px ;
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− | display:block;
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− | border-bottom: 1px solid #d3d3d3;
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− | font-weight:bold;
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− | cursor:pointer;
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− | -webkit-transition: all 0.4s ease;
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− | -moz-transition: all 0.4s ease;
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− | -ms-transition: all 0.4s ease;
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− | -o-transition: all 0.4s ease; transition: all 0.4s ease;
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− | }
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− | | + | |
− | .menu_item a {
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− | width: 100%;
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− | margin-left: -20px;
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− | padding: 11px 90px 12px 20px;
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− | text-decoration: none;
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− | color:black;
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− | }
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− | | + | |
− | /* When hovering on a menu item */
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− | .menu_items li:hover {
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− | background-color:#72c9b6;
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− | color: #000000;
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− | }
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− | | + | |
− | /* styling for the submenus */
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− | .submenu {
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− | width:100%;
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− | display: none;
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− | font-weight:bold;
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− | cursor:pointer;
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− | | + | |
− | }
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− | | + | |
− | /* moving the margin for the submenu ul list */
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− | ul.submenu {
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− | width: 100%;
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− | margin: 10px 0px -11px 0px;
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− | list-style: none;
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− | }
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− | | + | |
− | /*styling for the submenu buttons */
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− | .submenu li {
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− | width: 100%;
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− | margin-left: 10px;
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− | margin-bottom: 0px;
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− | }
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− | | + | |
− | | + | |
− | /* hover state for the submenu buttons */
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− | .submenu li a {
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− | width: 100%;
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− | padding: 5px 10px;
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− | display: inline-block;
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− | border-bottom: 1px solid #d3d3d3;
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− | background-color:white;
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− | text-decoration:none;
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− | color:#000000;
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− | }
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− | .submenu li a:hover {
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− | background-color:#000000;
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− | color: #72c9b6;
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− | }
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− | | + | |
− | | + | |
− | /* When the screen is smaller than 680px, the menu has the option to hide/show - this button controls that */
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− | .collapsable_menu_control {
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− | width:100%;
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− | padding: 15px 0px;
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− | display:none;
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− | background-color:#000000;
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− | cursor:pointer;
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− | -webkit-transition: all 0.4s ease;
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− | -moz-transition: all 0.4s ease;
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− | -ms-transition: all 0.4s ease;
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− | -o-transition: all 0.4s ease;
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− | transition: all 0.4s ease;
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− | }
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− | | + | |
− | /* when hovering on that button */
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− | .collapsable_menu_control:hover {
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− | background-color: #72c9b6;
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− | color:#000000;
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− | }
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− | | + | |
− | /********************************* CONTENT OF THE PAGE ********************************/
| + | |
− | | + | |
− | /* Wrapper for the content */
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− | .content_wrapper {
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− | width: 85%;
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− | margin-left:150px;
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− | padding:10px 0px;
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− | float:left;
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− | background-color:white;
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− | }
| + | |
− | | + | |
− | /*LAYOUT */
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− | .column {
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− | padding: 10px 0px;
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− | float:left;
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− | background-color:white;
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− | }
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− | .full_size {
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− | width:100%;
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− | }
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− | .full_size img {
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− | padding: 10px 15px;
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− | width:96.5%;
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− | }
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− | .half_size {
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− | width: 50%;
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− | }
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− | .half_size img {
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− | padding: 10px 15px;
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− | width: 93%;
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− | }
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− | .clear {
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− | clear:both;
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− | }
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− | .highlight {
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− | width: 90%;
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− | margin: auto;
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− | padding: 15px 5px;
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− | background-color: #f2f2f2;
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− | }
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− | | + | |
− | .judges-will-not-evaluate {
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− | border: 4px solid #72c9b6;
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− | display: block;
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− | margin: 5px 15px;
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− | width: 95%;
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− | font-weight:bold;
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− | }
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− | | + | |
− | | + | |
− | /*STYLING */
| + | |
− | | + | |
− | /* styling for the titles */
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− | .content_wrapper h1, .content_wrapper h2 {
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− | padding:5px 15px;
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− | border-bottom:0px;
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− | color:#72c9b6;
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− | | + | |
− | }
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− | .content_wrapper h3, .content_wrapper h4, .content_wrapper h5, .content_wrapper h6 {
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− | padding:5px 15px;
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− | border-bottom:0px;
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− | color: #000000;
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− | }
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− | | + | |
− | | + | |
− | /* font and text */
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− | .content_wrapper p {
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− | padding:0px 15px;
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− | font-size: 13px;
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− | font-family:Tahoma, Geneva, sans-serif;
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− | }
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− | .content_wrapper p2 {
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− | padding:0px 15px;
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− | font-size: 13px;
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− | font-family:Tahoma, Geneva, sans-serif;
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− | color: #FFFFFF
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− | }
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− | | + | |
− | /* Links */
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− | .content_wrapper a {
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− | font-weight: bold;
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− | text-decoration: underline;
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− | text-decoration-color:#72c9b6;
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− | color: #72c9b6;
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− | -webkit-transition: all 0.4s ease;
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− | -moz-transition: all 0.4s ease;
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− | -ms-transition: all 0.4s ease;
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− | -o-transition: all 0.4s ease;
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− | transition: all 0.4s ease;
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− | }
| + | |
− | | + | |
− | /* hover for the links */
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− | .content_wrapper a:hover {
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− | text-decoration:none;
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− | color:#000000;
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− | }
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− | | + | |
− | /* non numbered lists */
| + | |
− | .content_wrapper ul {
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− | padding:0px 20px;
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− | font-size: 13px;
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− | font-family:Tahoma, Geneva, sans-serif;
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− | }
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− | | + | |
− | /* numbered lists */
| + | |
− | .content_wrapper ol {
| + | |
− | padding:0px;
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− | font-size: 13px;
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− | font-family:Tahoma, Geneva, sans-serif;
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− | }
| + | |
− | | + | |
− | /* Table */
| + | |
− | .content_wrapper table {
| + | |
− | width: 97%;
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− | margin:15px 10px;
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− | border: 1px solid #d3d3d3;
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− | border-collapse: collapse;
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− | }
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− | | + | |
− | /* table cells */
| + | |
− | .content_wrapper td {
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− | padding: 10px;
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− | vertical-align: text-top;
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− | border: 1px solid #d3d3d3;
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− | border-collapse: collapse;
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− | }
| + | |
− | | + | |
− | /* table headers */
| + | |
− | .content_wrapper th {
| + | |
− | padding: 10px;
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− | vertical-align: text-top;
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− | border: 1px solid #d3d3d3;
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− | border-collapse: collapse;
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− | background-color:#f2f2f2;
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− | }
| + | |
− | | + | |
− | /* Button class */
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− | .button_click {
| + | |
− | margin: 10px auto;
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− | padding: 15px; width:12%;
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− | text-align:center;
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− | font-weight:bold;
| + | |
− | background-color: #72c9b6;
| + | |
− | cursor:pointer;
| + | |
− | -webkit-transition: all 0.4s ease;
| + | |
− | -moz-transition: all 0.4s ease;
| + | |
− | -ms-transition: all 0.4s ease;
| + | |
− | -o-transition: all 0.4s ease;
| + | |
− | transition: all 0.4s ease;
| + | |
− | }
| + | |
− | | + | |
− | /* button class on hover */
| + | |
− | .button_click:hover {
| + | |
− | background-color:#000000;
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− | color:#72c9b6;
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− | }
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− | | + | |
− | /********************************* RESPONSIVE STYLING ********************************/
| + | |
− | | + | |
− | /* IF THE SCREEN IS LESS THAN 1000PX */
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− | | + | |
− | @media only screen and (max-width: 1000px) {
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− | | + | |
− | #content {width:100%; }
| + | |
− | .menu_wrapper {width:15%;}
| + | |
− | .content_wrapper {margin-left: 15%;}
| + | |
− | .menu_item {display:block;}
| + | |
− | .icon {display:none;}
| + | |
− | .highlight {padding:10px 0px;}
| + | |
− | }
| + | |
− | | + | |
− | | + | |
− | /* IF THE SCREEN IS LESS THAN 680PX */
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− | | + | |
− | @media only screen and (max-width: 680px) {
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− | .collapsable_menu_control { display:block;}
| + | |
− | .menu_item {display:none;}
| + | |
− | .menu_wrapper { width:100%; height: 15%; position:relative;}
| + | |
− | .content_wrapper {width:100%; margin-left:0px;}
| + | |
− | .column.half_size {width:100%; }
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− | .column img { width: 100%; padding: 5px 0px;}
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− | .icon {display:block;}
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− | .highlight {padding:15px 5px;}
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− | }
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− | | + | |
− | </style>
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− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | <!--- THIS IS WHERE THE HTML BEGINS --->
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− | | + | |
− | | + | |
− | <!-- This tells the browser that your page is responsive -->
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− | | + | |
− | <head>
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− | <meta name="viewport" content="width=device-width, initial-scale=1">
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− | </head>
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− | | + | |
− | | + | |
− | <div>
| + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | <div class="collapsable_menu_control"> MENU ▤ </div>
| + | |
− | | + | |
− | <ul id="accordion" class="accordion">
| + | |
− | | + | |
− | | + | |
− | <li class="menu_item"> <div class="icon plus"></div> Competent Cells
| + | |
− | <ul class="submenu"> | + | |
− | <li> | + | |
− | <a href="">
| + | |
− | <div>
| + | |
− | <h>Preparation of Bacillus subtilis competent cells</h>
| + | |
− | | + | |
− | <ol><li>Streak out the strain to be made competent on an LB agar plate as a large patch and incubate overnight at 30°C</li>
| + | |
− | | + | |
− | <li>The following morning scrape the cell growth off the plate and use to inoculate fresh, pre-warmed, SpC medium (20 ml) to give an OD600 reading of about 0.5. </li> | + | |
− | | + | |
− | <li>Incubate the culture at 37°C with vigorous aeration and take periodic OD readings (OD600) to assess cell growth.</li> | + | |
− |
| + | |
− | <li>When the rate of cell growth is seen to depart from exponential (i.e. no significant change in cell density over 20-30 min) inoculate 200 ml of pre-warmed, SpII medium with 2 ml of stationary-phase culture and continue incubation at 37°C with slower aeration </li>
| + | |
− | | + | |
− | <li>After 90 min incubation, pellet the cells by centrifugation (8,000 g, 5min) at room temperature.</li>
| + | |
− | | + | |
− | <li>Carefully decant the supernatant into a sterile container and save.</li>
| + | |
− | | + | |
− | <li>Gently resuspended the cell pellet in 18 ml of the saved supernatant and add 2 ml of sterile glycerol; mix gently </li>
| + | |
− | | + | |
− | <li>Aliquot the competent cell (0.5 ml) in sterile tubes, freeze rapidly in liquid nitrogen or a dry-iced/ethanol bath or ice/isopropanol bath and store -70.</li></ol>
| + | |
− | | + | |
− | </div></a></li></ul></li>
| + | |
− | | + | |
− | | + | |
− | <li class="menu_item"> <div class="icon plus"></div> Transformation
| + | |
− | <ul class="submenu">
| + | |
− | <li>
| + | |
− | <a href="">
| + | |
− | <div>
| + | |
− | <h>Cells Preparation :</h>
| + | |
− | | + | |
− | <ol><li>Thaw competent cells rapidly by immersing frozen tubes in a 37°C water bath</li>
| + | |
− | | + | |
− | <li>Immediately, add one volume of SpII + EGTA to the Thawed cells; mix gently </li>
| + | |
− | | + | |
− | <li>In a sterile test tube add competent cell (0.2~0.5 ml) to the DNA solution (<0.1 ml) and incubate in a roller drum at 37.</li>
| + | |
− | | + | |
− | <li>Dilute the transformed cells as appropriate in T base containing 0.5% glucose and plate immediately onto selective media. </li></ol>
| + | |
− | | + | |
− | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Transfo_pic_1.png" width="400px"/></p>
| + | |
− | </div></a>
| + | |
− | <a href=""><div>
| + | |
− | <h>Digestion :</h>
| + | |
− | <ol><li>Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. </li>
| + | |
− | <p>In a 1.5mL tube combine the following:</p>
| + | |
− | <ul><li>DNA </li>
| + | |
− | <li>Restriction Enzyme(s)</li>
| + | |
− | <li>Buffer </li>
| + | |
− | <li>dH2O up to total volume</li> </ul>
| + | |
− | <p>Mix gently by pipetting. </p>
| + | |
− | | + | |
− | <li>Incubate tube at appropriate temperature (usually 37°C) for 1 hour. </li>
| + | |
− | | + | |
− | <li>Always follow the manufacturer’s instructions. </li>
| + | |
− | | + | |
− | <li>To visualize the results of your digest, conduct gel electrophoresis</li></ol>
| + | |
− | </div></a>
| + | |
− | <a href=""><div>
| + | |
− | <h>Vector Preparation :</h>
| + | |
− | <p>Combine the following in a PCR or Eppendorf tube:</p>
| + | |
− | <ul><li>25ng Vector DNA</li>
| + | |
− | <li>75ng Insert DNA</li>
| + | |
− | <li>Ligase Buffer (1μL/10μL reaction for 10X buffer, and 2μL/10μL reaction for 5X buffer)</li>
| + | |
− | <li>0.5-1μL T4 DNA Ligase</li>
| + | |
− | <li>H20 to a total of 10μL</li></ul>
| + | |
− | <p>Incubate at room temperature for 2hr, or at 16°C overnight (following the manufacturer’s instructions).</p>
| + | |
− | | + | |
− | </div></a></li></ul></li>
| + | |
− | | + | |
− | | + | |
− | | + | |
− | <li class="menu_item"> <div class="icon plus"></div> Deletion
| + | |
− | <ul class="submenu">
| + | |
− | <li>
| + | |
− | <a href="">
| + | |
− | <div>
| + | |
− | <p>Deletion Step 1 :</p>
| + | |
− | <img src="aaa" width="500px"/>
| + | |
− | <p>Deletion Step 2 :</p>
| + | |
− | <img src="aaa" width="500px"/>
| + | |
− | <p>Deletion Step 3 :</p>
| + | |
− | <img src="aaa" width="500px"/></div></a></li></ul></li>
| + | |
− | | + | |
− | | + | |
− | <li class="menu_item"> <div class="icon plus"></div> Other
| + | |
− | <ul class="submenu">
| + | |
− | <li>
| + | |
− | <a href="">
| + | |
− | <div>
| + | |
− | <p>Whatever Step 1 :</p>
| + | |
− | <img src="aaa" width="50px"/>
| + | |
− | <p>Whatever Step 2 :</p>
| + | |
− | <img src="aaa" width="50px"/></div></a>
| + | |
− | <a href="">
| + | |
− | <div>
| + | |
− | <p>Un autre truc Step 1 :</p>
| + | |
− | <img src="aaa" width="50px"/>
| + | |
− | <p>Un autre truc Step 2 :</p>
| + | |
− | <img src="aaa" width="50px"/></div></a></li></ul></li>
| + | |
| | | |
| + | </div> |
| | | |
| + | <div class="column half_size"> |
| + | <h5>Inspiration</h5> |
| + | <ul> |
| + | <li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li> |
| + | <li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li> |
| + | <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li> |
| </ul> | | </ul> |
− |
| |
| </div> | | </div> |
| | | |
| | | |
| + | <div class="clear"></div> |
| | | |
| | | |
| + | <div class="column half_size"> |
| | | |
− | <div class="content_wrapper">
| |
| | | |
| | | |
− | | + | </div> |
− | <h1 id="team_name"> </h1>
| + | |
− | <h4 id="page_name"> </h4>
| + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | <script>
| + | |
− | | + | |
− | // This is the jquery part of your template. Try not modify any of this code since it makes your menu work.
| + | |
− | | + | |
− | $(document).ready(function() {
| + | |
− | | + | |
− | $("#HQ_page").attr('id','');
| + | |
− |
| + | |
− | | + | |
− | if ( wgPageName.substring( 0, 8) == "Template") { // if the page is a template it displays the full name in a single line
| + | |
− | $("#team_name").html( wgPageName );
| + | |
− | }
| + | |
− | | + | |
− | else if ( ( (wgPageName.match(/\//g) || []).length ) == 0 ) { // if it is the home page , just print the team's name
| + | |
− | $("#team_name").html( wgPageName.substring( 5, wgPageName.length ) );
| + | |
− | }
| + | |
− | | + | |
− | else {
| + | |
− | // this adds the team's name as an h1
| + | |
− | $("#team_name").html( wgPageName.substring( 5 , wgPageName.indexOf("/") ) );
| + | |
− | | + | |
− | // this adds the page's title as an h4
| + | |
− | $("#page_name").html ( ( wgPageName.substring( wgPageName.indexOf("/") + 1, wgPageName.length ) ).replace( /\/|_/g , " ") );
| + | |
− | }
| + | |
− | | + | |
− | | + | |
− | if( ($(location).attr('href')).indexOf("submit") >= 0 ) {
| + | |
− | $(".menu_wrapper").css( { "position": "absolute", "height": "auto"});
| + | |
− | $("#wpTextbox1").css( 'margin-top', '440px');
| + | |
− | }
| + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | $('#accordion').find('.menu_item').click(function(){
| + | |
− | | + | |
− | //Expand or collapse this panel
| + | |
− | submenu = $(this).find('.submenu');
| + | |
− | submenu.toggle();
| + | |
− | | + | |
− | icon = $(this).find('.icon');
| + | |
− | | + | |
− | if ( !$( submenu ).is(':visible') ) {
| + | |
− | icon.removeClass("less").addClass("plus");
| + | |
− | }
| + | |
− | else {
| + | |
− | icon.removeClass("plus").addClass("less");
| + | |
− | }
| + | |
− | | + | |
− | //Hide the other panels
| + | |
− | $(".submenu").not(submenu).hide();
| + | |
− | $(".icon").not(icon).removeClass("less").addClass("plus");
| + | |
− | });
| + | |
− | | + | |
− | | + | |
− | $(".collapsable_menu_control").click(function() {
| + | |
− | $(".menu_item").toggle();
| + | |
− | });
| + | |
− | | + | |
− | $( window ).resize(function() {
| + | |
− | $(".menu_item").show();
| + | |
− | });
| + | |
− | | + | |
− | | + | |
− | });
| + | |
− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | </script> | + | |
− | | + | |
| | | |
| </html> | | </html> |