Difference between revisions of "Team:CU-Boulder/Notebook"
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<b> <h3 id="top"> Notebook <h3> </b> | <b> <h3 id="top"> Notebook <h3> </b> | ||
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| − | <h2> Week 1 </h2> | + | <h2> Week 1 5/8/16-5/14/16</h2> |
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| − | <h2> Week 2 </h2> | + | <h2> Week 2 5/15/16-5/21/16</h2> |
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| + | <figure style="float:right"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/f/fa/T--CU-Boulder--19gel.jpeg">Fig.1 - Lane 2: eGFP-Hi 1c3 Lane 3: eGFP-Hi 1c3 Lane 4: ladder Lane 5: eGFP-Lac 1c3 Lane 6: eGFP-Lac 1c3</figcaption> | ||
| + | <!--<p style="margin-left:100px"> | ||
| + | The caption text is a pain | ||
| + | </p> --> | ||
| + | </figure> | ||
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Revision as of 17:46, 9 August 2016
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Notebook
Week 1 5/8/16-5/14/16
Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.
Week 2 5/15/16-5/21/16
Fig.1 - Lane 2: eGFP-Hi 1c3 Lane 3: eGFP-Hi 1c3 Lane 4: ladder Lane 5: eGFP-Lac 1c3 Lane 6: eGFP-Lac 1c3
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Week 3
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Week 4
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Week 5
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Week 6
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Week 7
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Week 8
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Week 9
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Week 10
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Week 11
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Week 12
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Week 13
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Week 14
Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.
Week 15
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Week 16
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